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                    <h3>Integrated HP</h3>

                    <ul>

                        <li><a class="fa fa-plug" href="#inspiration">&nbsp;Content</a>

                            <ul>

                                <li><a href="#inspiration"><b>· Inspiration & Investigation</b></a></li>

<!--                                        <li><a href="#overview_2">&emsp;- Overview</a></li>-->

                                <li><a href="#implementation"><b>· Implementation</b></a></li>

<!--                                        <li><a href="#overview_3">&emsp;- Overview</a></li>-->

                                <li><a href="#application"><b>· Application</b></a></li>

<!--                                        <li><a href="#overview_4">&emsp;- Overview</a></li>-->

                                <li><a href="#2"><b>· Further Publicity</b></a></li>

                                <ul>

                                    <li><a href="#2-1">&emsp;- High School Lecture</a></li>

                                    <li><a href="#2-2">&emsp;- Summer Camp Activities</a></li>

                                    <li><a href="#2-3">&emsp;- Debate Competition</a></li>

                                    <li><a href="#2-4">&emsp;- Primary School Lecture</a></li>

                                    <li><a href="#2-5">&emsp;- Postcards about Nutriology</a></li>

                             </ul>

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                <a class="anchor" id="overview_1"></a>

                <h1 class="mt-5" >Overiew</h1>

                <p>In this year’s human practices, we took diverse kinds of activities to promote the development of our

                    project and make our contribution to the world.</p>

                <p>In the preparation stage, with the talk to Dr. Chen Xiaopeng, we had a general understanding of the

                    current situation of insomnia. The interview with Prof. Zhang Yali guided us to think of the idea of

                    designing a milk machine with the function of producing tryptophan itself. </p>

                <p>For our implementation stage, the interview with Prof.Xu Dan and Dr. Liu Ziqiang provided us with

                    precious suggestions for our wet-lab suggestions, which helped us to solve the problems we came up

                    with during these periods.</p>

                <p>The talk with Dr.Zong Yeqing benefited us a lot in polishing our design of hardware during the

                    application stage. And the investigation we did enabled us to view the application of our projects

                    from the perspective of our proposed users.</p>

                <p>On top of these, we also endeavored to make broader contribution to the public by holding diverse kinds

                of meaningful activities like lecture about synthetic biology, debate competition focused on bio-safety

                    and ethic even delicate postcards providing nutrition knowledge.</p>

                <p>All In all, our various HP activities benefit the whole stages (inspiration, implementation and

                    application stages) of our project. Furthermore, it also contribute to the public and the world</p>

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                <h1 class="mt-5" >Content</h1>

                <a class="anchor" id="inspiration"></a>

                <h2 class="mt-5" >Inspiration and background investigation</h2>

                <a class="anchor" id="overview_2"></a>

                <p ><b>Overview:</b> In the preparation stage of the project, we carried out a wide range

                    of background investigations to confirm the feasibility of using tryptophan to release the pain of

                    insomnia. We interviewed with Prof.Zhang Yali, in order to find out the possible ways of using

                    tryptophan to cure insomnia. After the deep interaction with Prof.Zhang, we decided to explore

                    the possibility of using milk as medium to help people absorb extra tryptophan. And to make the

                    step further, considering the background of epidemic, the idea of constructing a domestic milk

                    machine to fulfill our goal attracted our attention successfully. Meanwhile, we were honored to

                    have a deep communication with Dr. Chen Xiaopeng, who had much experience in curing patients with

                    mental health problems like sleep disorder. The talk covered a lot of useful contents: from the

                    current situation and therapies of insomnia, to the feasibility of using tryptophan to cure

                    insomnia. Dr. Chen even inspired us to do some popularization of science to introduce the insomnia

                    and its solutions towards public.</p>

                <a class="anchor" id="point_1"></a>

                <h3 class="mt-5" >I. Interview with Prof.Zhang Yali</h3>

                <div align="center"><img src="https://static.igem.org/mediawiki/2021/6/64/T--XJTU-China--2-ZYL-Photo.png"

                    alt="Pohto of Prof. Zhang" width="20%" ></div>

                <p>

                    In order to understand the anxiety problems and sleep conditions in the context of the epidemic and

                    to learn to choose the suitable medium to carry our tryptophan, we interviewed Prof. Zhang Yali from

                    the School of Life Science of Xi'an Jiaotong University.</p>

                <p>Prof. Zhang introduced us the interdisciplinary intersection of microbiology and food nutrition with

                    the fermentation industry as the core, affirming the broad prospects of microbiology and synthetic

                    biology.She mentioned that under the social background of epidemic, making difference to people’s

                    diet is more safer and convenient. Pro. Zhang alos discussed with us the selection of engineering

                    bacteria and explore the value of tryptophan in various applications.</p>

                <p>At the same time, we were also glad to know that tryptophan has much wider application in

                    diverse area than we used to think, which can be used to help people release their feeling of

                    anxiety and depression. And further investigations proved this idea.</p>

                <p>With the guidance and suggestions, we chose to use food medium to carry our tryptophan, and would

                    focus on the issues of product acceptance and safety came up with idea to use milk reserved in a

                    domestic.</p>

                <div align="center"><img src="https://static.igem.org/mediawiki/2021/7/7f/T--XJTU-China--3-ZYL-Interview.png"

                                        alt="Interview with Prof. Zhang" width="90%" ></div>

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                    <p>Under the circumstance of recurring epidemic, mental health problems caused by reduced socialization,

                        like increased anxiety and widespread sleep problems receive much attention from the public. And due

                        to our goal of helping people solve these problems by making difference to their diet, we actually

                        need the interaction with expert in the area of nutrition and food health. Luckily , our team had a

                        chance to interview Pro. Zhang Yali from the School of Life Sciences at Xi'an Jiaotong University,

                        who has profound knowledge of nutriology. We realized that we can meet human health needs with the

                        help of food nutrition and microbiology, while gaining a deeper understanding of our tryptophan

                        project.</p>

                    <p>Prof. Zhang has long been engaged in microbiology and food nutrition research, so she began by

                        introducing us the most central part of the intersection of the two fields: the fermentation

                        industry supported by the microbiology discipline. For the purpose of producing natural products

                        using genetic engineering techniques, there are both advantages and disadvantages compared to the

                        direct use of chemical synthesis. At present, there are more than 80 polypeptide drugs available

                        worldwide, which are obtained by three methods: chemical synthesis, genetic recombination and plant

                        or animal extraction. There are still extreme challenges in the process of developing new

                        polypeptide drugs. Meanwhile, the strict regulations to their application are supposed to be

                        considered.</p>

                    <p>In the post-epidemic era, individuals are paying more attention to their own health conditions.

                        Prof. Zhang believed that this trend provides a broad prospect for the development of microbiology

                        and synthetic biology, in order to meet the needs of the public. And microorganisms have pivotal

                        impact on human health. First of all , microorganisms have coexisted with humans for a long time in

                        our intestinal, oral and skin flora, which have a long-lasting and stable impact on human

                        homeostasis and immunity. On top of that, microorganisms can also affect human indirectly by

                        influencing our living environment. Therefore, the research in the direction of the human microbiome

                        is also crucial to be further explored.</p>

                    <p>Finally, we and Prof.Zhang focus on the design and idea of our project. In terms of hardware design,

                        Prof. Zhang suggested us to grasp the selection of engineered bacteria to ensure the sensory

                        properties of the product and its curative effect are accepted. At the same time, tryptophan, as a

                        precursor substance ,may not be as effective as melatonin in the short term. But as a component of

                        food and more importantly, the essential amino acids for human life, its longevity and organism

                        stability are much better. Moreover, tryptophan is involved in numerous metabolic processes, and the

                        injection of it is of great clinical value in treating diseases like hypertension and depression.

                        Combined with iron and vitamins, tryptophan can intervene in locomotor anemia; the combination with

                        histidine can treat peptic ulcers. Therefore, the prospect for the application and development of

                        tryptophan is really wide.</p>

                    <p>With the guidance and advice of Prof. Zhang, we learned that tryptophan has a broad application

                        prospect, at the same time,we needed to focus on product acceptance and safety issues in hardware

                        development.</p>

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                <h3 class="mt-5" >II. Interview with Dr.Chen Xiaopeng</h3>

                <p>In order to gain insight into the causes and treatment of insomnia today and explore new ideas for

                    sleeping aid, our team contacted and interviewed Dr. Chen Xiaopeng, Deputy Chief Physician of the

                    Department of Neurology at the People's Hospital of Jiangnan University.</p>

                <p>We consulted Dr. Chen about the main factors that trigger insomnia, the characteristics of patients

                    in clinical work and the treatment methods used at present. He provided us with the related

                    information with his own clinical experience, which helped us have a comprehensive overview of the

                    current situation of insomnia.</p>

                <p>At the same time, Dr. Chen affirmed our team’s idea of using tryptophan as a sleep-aid molecule

                    produced by a household device. And he also gave suggestions for solving the insomnia problem

                    among young people, which inspires us to do more popularization of science related to mental health

                    to the public.</p>

                <p>Through this interview and investigation, we gain a new comprehension of insomnia and determined that

                    the home-based production of tryptophan will be realized through synthetic biology and home-based

                    hardware design.</p>

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                    <p>By distributing and collecting the questionnaires about the degree of insomnia of the public, we come

                        to a conclusion that insomnia is a general and common situation in the present society. Therefore,

                        in order to gain a deeper understanding of the causes and solutions to improve the current insomnia

                        situation, we interviewed Dr. Chen Xiaopeng, Deputy Chief Physician of the Department of Neurology

                        at the People's Hospital of Jiangnan University, and discussed the feasibility of our project using

                        tryptophan as a nutritional supplement. Through this interview and research, we learned a lot about

                        the causes of insomnia symptoms and the ways to treat them.</p>

                    <p>First, he introduced us the factors that cause insomnia in nowadays society. In terms of the

                        objective reasons, individuals’ age, gender, family history and personality may contribute to

                        insomnia. From the point of subjective side, some individuals get into insomnia due to the

                        disruption of their life rhythms, while some others are forced by competitive pressure from their

                        work or life. Meanwhile, Dr. Chen told us that the proportion of adults suffering from insomnia in

                        mainland China is as high as 57%, with the majority of chronic insomnia patients seeking medical

                        attention concentrated in middle-aged and elderly people, while young people are not as willing to

                        seek medical treatment when they develop insomnia symptoms.</p>

                    <p>Dr. Chen has also provided valuable suggestions for our project. He mentioned that the standardized

                        therapies for chronic insomnia at present include psychotherapy, pharmacotherapy, physiotherapy,

                        Chinese medicine, and integrative treatment. And our team's choice of using tryptophan as a

                        nutritional supplement belongs to over-the-counter medication in pharmacotherapy. Although the

                        clinical application of tryptophan is controversial, a recent Meta-analysis by the National

                        University of Singapore on the effect of tryptophan on sleep disorders indicated that adequate

                        supplementation of tryptophan can significantly reduce the time to fall asleep. As a consequence,

                        the direction of our project is worthy of trying and exploring.</p>

                    <p>Finally, Dr.Chen offered some suggestions to prevent insomnia for today’s youth. To start with

                        subjective causes, many surveys have shown that common triggers of insomnia among students

                        include excessive use of electronic products such as cell phones, smoking, drinking alcohol or milk

                        tea, learning pressure and emotional instability. Therefore, Dr. Chen suggested students to pay

                        attention to overcoming these factors in their lives. In addition, scientific research also

                        indicates that melatonin secretion in the human body starts at about 9:00 pm and gradually

                        decreases to stop after about 12:00 pm. The gastrointestinal motility of the digestive system stops

                        at about 10:30 pm, which reminds us that we should arrange the pace of life reasonably.</p>

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                <a class="anchor" id="implementation"></a>

                <h2 class="mt-5" >Implementation</h2>

                <a class="anchor" id="overview_3"></a>

                <p ><b>Overview:</b> In the application stage, we tried our best to broaden the prospect

                    of our project. After the talk with Dr. Liu Ziqiang, we decided to try to pay a visit to fermentation

                    factory. And finally, we contacted with a alcohol fermentation factory to get some experience of

                    bacteria fermentation. With the original design of our hardware, we made connection with

                    Dr. Zong Yeqi. Dr. Zong evaluated our designs and pointed out the potential challenges. On top of

                    that, he eagerly gave us much advice to help us make our design more reliable and user-friendly.

                    He also mentioned that it would be better for us to polish our hardware if we can carry out public

                    survey to collect first-hand data. As a result, we did a public survey to evaluated the current

                    situation of insomnia, as well as the acceptability of our hardware design. The outcome of the

                    survey showed that public awareness of insomnia needs to be strengthen. And surprisingly, nearly 95%

                    of investigated population held an open mind to our hardware and were willing to try it if the

                    technology is mature enough.</p>

                <a class="anchor" id="point_3"></a>

                <h3 class="mt-5" >III. Interview with Prof.Xu Dan</h3>

                <div align="center"><img src="https://static.igem.org/mediawiki/2021/8/86/T--XJTU-China--4-XD-Photo.png"

                                        alt="picture of Prof. Xu" width="20%" ></div>

                <p>We conducted a communication in HP with NWU-CHINA-A team, and jointly interviewed Associate Professor

                    Xu Dan of Xi’an JiaoTong University to deeply explore the application of synthetic biology in the

                    project.</p>

                <p>Prof. Xu answered our concerns about the success of exogenous gene expression in host microorganisms

                    in synthetic biology project, and introduced the current challenges regarding the application of

                    synthetic biology methods to achieve industrial mass production. She also convinced us that

                    synthetic biology has broad application prospects and more specifically, she provided us with

                    suggestions during our wet-lab experience.</p>

                <p>In the next step, our project would continue to focus on the purification of synthetic biological

                    products and yield issues.</p>

                <div align="center"><img src="https://static.igem.org/mediawiki/2021/d/dd/T--XJTU-China--NWU-10.png"

                                        alt="Interview with Prof. Xu" width="90%" ></div>

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                    <p>Our team had a communication with NWU-CHINA-A team about HP. And we interviewed Associate Professor

                        Xu Dan from Xi'an Jiaotong University. Prof. Xu has specialized in the field of microbiology for

                        many years, and during the cooperation, we clarified the promising application of bacterium

                        production of tryptophan, and gained further understanding of the key points for bacterium

                        production.</p>

                    <p>First of all, Professor Xu introduced us a problem in synthetic biology projects nowadays: whether

                        exogenous genes can be successfully expressed in host microorganisms. The main reasons exist in the

                        situations like codon preference, toxicity of the exogenous gene expression product, and structural

                        differences in the protein expression product among species. Fortunately, there are some

                        corresponding technologies to these situations. Then, Prof. Xu answered our questions about the

                        application of synthetic biology in achieving industrial mass production. Although industrial mass

                        production needs to take factors like gene component assembly, splicing and expression product

                        selection, expression efficiency evaluation, expression product purification, etc into

                        consideration, it is a topic with great potential and investment value. So, it is worthy of time and

                        energy we spared to learn and master. Finally, Prof. Xu stressed that synthetic biology is a field

                        with lots of "down-to-earth" inventions that will benefit our life in a comprehensive way. And in

                        the future, the achievement in synthetic biology will continue to gain popularity and attention in

                        real life for its convenience and wide-spread use.</p>

                    <p>Through the interview with Prof. Xu, we have a better understanding of the field of synthetic

                        biology. In our project, we will continue to focus on the purification of synthetic biology products

                        and the yield issues.</p>

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                <h3 class="mt-5" >IV. Interview with Dr.Liu Ziqiang</h3>

                <div align="center"><img src="https://static.igem.org/mediawiki/2021/4/40/T--XJTU-China--17-LZQ-Photo.jpeg"

                                        alt="Photo of Dr. Liu" width="25%" ></div>

                <p>In order to solve the problems concerning fermentation device design and learn more about tryptophan

                    production, we consulted Dr. Liu. </p>

                <p>Dr. Liu eagerly shared with us the information about the specific experimental process, some notices

                    in fermentation process and the possibility of using periodic bacteria package, with his own

                    experience in the research of fermentation of tryptophan. On the premise of ensuring the high

                    quality and continuous production of tryptophan, Dr. Liu gave us some suggestions on the design of

                    fermentation devices and evaluated our crafts for machine. We also discussed the current situation,

                    improvement in efficiency of tryptophan large-scale industrial production. </p>

                <p>After conversation with Dr. Liu, we gained an in-depth understanding of the main points in the

                    fermentation experiment and the industrial background of the project. Furthermore, Dr. Liu's

                    guidance on hardware also had great significance.</p>

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                <div id="more4" style="height: 0; overflow: hidden">

                    <p>To gain an in-depth understanding of the key points of the fermentation experiment, we interviewed

                        Dr. Liu Ziqiang, an expert in fermentation from Bluepha Lab. We gained more understanding of the

                        industrial background of our project. In addition, Dr. Liu's guidance on hardware also benefited us

                        significantly. </p>

                    <p>First of all, Dr. Liu reminded us to pay attention to the sterility of the fermentation system,

                        including raw material sterility, process sterility, and the fermentation cycle for thoroughbred

                        culture. Therefore, more stricter regulations should be applied to the control of substrate

                        nutrition, intermediate process, the medium, and the process of conditional parameter control.</p>

                    <p>For the "tea-making mode" we mentioned (to use recyclable replacement of the bacteria package)

                        Dr. Liu thought that the operation is more complex than we expected. Considering the tiny size of

                        E. coli, the aperture of required membrane should meet the requirements of 0.2 to 0.3 microns to

                        prevent E. coli from entering the beverage liquid, which subsequently leads to the challenge of

                        separation process. Secondly, in the design of fermentation device, Dr. Liu suggested considering

                        the compatibility of function and volume. However, the current problem is that the material

                        transport between different accessories in the device mainly relies on pressure differential

                        method and pumps, so it’s hard to achieve the goal of “both small and multi-functional”.

                        Apart from that, striking a balance between costs and consumers is quite hard. If high techniques

                        are used, it undoubtedly leads to increasing price, which is hard to accept by customers.</p>

                    <p>Finally, he talked about the present situation of tryptophan production. Amino acid products, as a

                        food additive, have been widely used in the field of food and health care with huge demand and

                        market. Specifically, the maximum annual production of the company with mature tryptophan

                        fermentation technology is about 10,000 tons. However, after purification, there is a 20% to 30%

                        loss in tryptophan fermentation liquid, which leads the total conversion rate stay at about 10%, far

                        less than that of glutamate (60-70%). The key to improve tryptophan fermentation technology is to

                        improve strains we use. Selecting more appropriate strains can largely improve conversion rates,

                        releasing burden of purifying.</p>

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                    <a onclick="readmore4()"><p><b>[CLICK TO READ MORE]</b></p></a>

                </div>

                <a class="anchor" id="application"></a>

                <h2 class="mt-5" >Application</h2>

                <a class="anchor" id="overview_4"></a>

                <p ><b>Overview:</b> In the application stage, we tried our best to broaden the prospect

                    of our project. After the talk with Dr. Liu Ziqiang, we decided to try to pay a visit to

                    fermentation factory. And finally, we contacted with an alcohol fermentation factory to get some

                    experience of bacteria fermentation. With the original design of our hardware, we made connection

                    with Dr. Zong Yeqi. Dr. Zong evaluated our designs and pointed out the potential challenges. On top

                    of that, he eagerly gave us much advice to help us make our design more reliable and user-friendly.

                    He also mentioned that it would be better for us to polish our hardware if we can carry out public

                    survey to collect first-hand data. As a result, we did a public survey to evaluate the current

                    situation of insomnia, as well as the acceptability of our hardware design. The outcome of the

                    survey showed that public awareness of insomnia needs to be strengthen. And surprisingly, nearly 95%

                    of investigated population held an open mind to our hardware and were willing to try it if the

                    technology is mature enough. </p>

                <a class="anchor" id="point_5"></a>

                <h3 class="mt-5" >V. Interview with Dr.Zong Yeqing</h3>

                <div align="center"><img src="https://static.igem.org/mediawiki/2021/e/ee/T--XJTU-China--6-ZYQ-Photo.png"

                                        alt="Picture of Prof. Zong" width="20%" ></div>

                <div id="more5" style="height: 0; overflow: hidden">

                         interviewed Dr. Zong from Bluepha Lab and discussed our design ideas for creating a hardware of

                        tryptophan producing. First of all, Dr. Zong gave his advice on the feasibility of fermenting drinks

                        through portable device and then extracting tryptophan to make drinks. He explained that

                        bio-fermentation is still a main source of producing tryptophan, so what we need to focus now is

                        selecting strains with high productivity, which subsequently leads to the maximum tryptophan

                        yield.</p>

                    <p>In addition, Dr. Zong taught us the reasonable methods of analyzing the feasibility of our hardware.

                        First, we need to know the highest concentration of tryptophan at present, and then design strains

                        and systems based on it. Next, the hardware should be designed from this point of view.And finally,

                        we should analyze the results and causes. Secondly, Dr. Zong talked about the need to design

                        hardware products from the angle of customers, as far as possible to meet the needs of

                        miniaturization and simplicity, that’s what we call user-friendly design. Also, designing the

                        hardware originally, being innovative and making improvement based on the previous version are

                        vital.</p>

                    <p>Surprisingly, Dr. Zong also gave us a few suggestions for doing HP. First, members in Hp group needs

                        to demonstrate the importance of our design for everyday life and industrial production. The second

                        suggestion is adding more field trips into HP activities, which can help us accumulate enough data

                        and increase the credibility of our design. In short, through the conversation with Dr. Zong, we had

                        a deeper understanding of the design and development of hardware, which is of great importance in

                        home-based hardware design.</p>

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                    <a onclick="readmore5()"><p><b>[CLICK TO READ MORE]</b></p></a>

                </div>

                <a class="anchor" id="point_6"></a>

                <h3 class="mt-5" >VI. Visit to the Shaanxi Qinyang Changsheng Liquor Factory</h3>

                <div align="center"><img src="https://static.igem.org/mediawiki/2021/4/49/T--XJTU-China--8-Visit-1.png"

                                        alt="Visit Photo 1" width="90%" ></div>

                <p>In order to address the issues during the fermentation of tryptophan including bacterial

                    contamination, strain passage and product purification, we decided to pay a visit to the related

                    factories. But the restriction of epidemic had a great impact on our plan. After contacting for a

                    long time and considering the possibility, we went on a field trip to tour the Shaanxi Qinyang

                    Changsheng Liquor Co., Ltd, to find the similar methods we may apply in our projects. </p>

                <p>We interviewed Mr. Wang Ping and Mr. Wu Dongping there, and drew on their experience concerning

                    technology used in industrial brewing process.</p>

                <div align="center"><img src="https://static.igem.org/mediawiki/2021/5/5b/T--XJTU-China--9-Visit-2.png"

                                        alt="Visit Photo 2" width="90%" ></div>

                <p>First of all, we visited the whole production line with the staff’s guide. Mr. Wang and Mr. Wu

                    pointed out that the qualified personnel, the control of technological skills and equipment

                    sanitization are the requisites for contamination avoidance. In addition, since fermentation is

                    literally a process with complex mixtures of various microbes, it is not realistic to circumvent all

                    the pollutant. We also gained insight into the separation techniques and the way to maintain

                    microbiological stable (by controlling the key strains in different techniques). Furthermore, to

                    ensure the quality of fermented products, it is vital to create a condition (temperature, humidity

                    etc.) suitable for microbes.</p>

                <div align="center"><img src="https://static.igem.org/mediawiki/2021/9/99/T--XJTU-China--10-Visit-Interview.png"

                                        alt="Visit Interview" width="90%" ></div>

                <p>Mr. Wang and Mr. Wu also pointed out that one of the main research directions in this filed is

                    removing the mycotoxin, which is potentially responsible for contamination out of the fermentation

                    system, though Qinyang Changsheng Liquor Co., Ltd doesn’t have a process relating to it.</p>

                <a class="anchor" id="point_7"></a>

                <h3 class="mt-5" >VII. A survey about insomnia and public’s attitude toward our design of

                    hardware</h3>

                <p>A survey was carried out to understand the sleep status, problems and people’s attitudes towards

                    microbial fermentation of producing sleep-aid products and home production instruments. The

                    respondents we surveyed were all ages, with a total of 1,020. </p>

                <p>Obviously, the information we collected from the public actively polish our design ideas and reveal

                    the potential obstacles we may face when we try to make our design down to earth. What’s more, the

                    result also helps us to precisely evaluate the market need for our hardware, in order to make our

                    project go further.</p>

                <p><b>The result about sleep quality of people shows two problems</b></p>

                <p>1. More than half investigated individuals are suffered from sleep problems:</p>

                <div class="row mt-5">

                    <div class="col-6">

                        <div class="imgWrapper centerize">

                            <img src="https://static.igem.org/mediawiki/2021/c/c1/T--XJTU-China--11-Chart-1-Newest.png"

                                alt="Fig. 1" width="90%">

                            <span class="description"><strong>Fig.1</strong> Whether people had problems about their

                                sleep in the past few months</span>

                    <div class="col-6">

                        <div class="imgWrapper centerize">

                            <img src="https://static.igem.org/mediawiki/2021/b/ba/T--XJTU-China--12-Chart-2-New.png"

                                alt="Fig. 2" width="90%">

                            <span class="description"><strong>Fig.2</strong> the sleep loss frequency for people who

                                sufferfrom insomnia</span>

                <p>More than 60% of the respondents have insomnia in the past month, more than 60% of them suffered from

                    insomnia over 3 times in a month, and more than 20% of them suffered from insomnia over 9 times in a

                    month. Long time of falling asleep, poor sleep quality and short sleep time are common problems for

                    insomniacs. And according to Pearson-related analysis, sleep status is not related to age or gender,

                    which means that people of all ages suffer from insomnia. (The results of the analysis are detailed

                    in "Relevance between gender, age and sleep status")</p>

                <p>2. The traditional treatment of sleep problems has achieved little success:</p>

                <div class="imgWrapper centerize">

                    <img src="https://static.igem.org/mediawiki/2021/b/be/T--XJTU-China--13-Chart-3-New.png"

                        alt="Fig. 3" width="90%">

                    <span class="description"><strong>Fig.3</strong> Different methods to deal with insomnia and their

                        effect</span>

                </div>

                <p>Although a great deal of people suffer from insomnia, only 17.97% of these people have tried to treat

                    it. And the survey results show that hospital visits, drug sleep aids, physical sleep aids, and

                    popular sleep aids account for only about 15% on average. It can be seen that most insomniacs' sleep

                    problems have not improved after treatment.</p>

                <p><b>The result about microorganism fermentation show two results</b></p>

                <div class="imgWrapper centerize">

                    <img src="https://static.igem.org/mediawiki/2021/6/6c/T--XJTU-China--14-Chart-4-2-Newest.jpeg"

                        alt="Fig. 4" width="90%">

                    <span class="description"><strong>Fig.4</strong> People’s understanding of microorganism and

                        fermentation and its use in nowadays society</span>

                </div>

                <p>1. People know little about microbial fermentation of producing drugs</p>

                <p>According to the surveys, only 17.63% of people have a certain understanding of Escherichia coli's

                    ability to produce drugs, and only 9.86% have a certain understanding that tryptophan has

                    sleep-assisting effect. According to the Pearson-related analysis, there is a positive correlation

                    between the acceptance of sleep aid products of microbial fermentation products and the

                    understanding of Escherichia coli and tryptophan (see "Acceptance of microbial fermentation and

                    understanding of bacteria and tryptophan for details of the analysis results).And the acceptance

                    rate is also positively related to age and the attempt to treat insomnia (analysis results are

                    detailed in "Analysis of Microbial Product Acceptance and Age). It shows that more knowledge about

                    microbial fermentation drugs and insomnia treatment needs to be popularized to people to improve

                    acceptance.</p>

                <p>2. There is a broad prospect for Tryptophan produced by fermentation in aiding sleep: </p>

                <p>According to the survey results, 61.74% of the people have tried to solve insomnia through dietary

                    therapy, and about 90% of people are reserved and acceptable in the face of sleeping-aid food

                    produced by microbial fermentation. It can be seen that most people may accept food containing

                    fermented tryptophan to improve sleep. Producing Tryptophan by fermentation within a domestic

                    machine is worth trying and will have its own audiences</p>

                <p><b>The investigation about hardware show a tendency</b></p>

                <p>1. High acceptance of domestic machine to produce Tryptophan: </p>

                        <div class="imgWrapper centerize">

                            <img src="https://static.igem.org/mediawiki/2021/9/92/T--XJTU-China--15-Chart-5-New.png"

                                alt="Fig. 5" width="90%">

                            <span class="description"><strong>Fig.5</strong> People’s attitude toward the domestic

                                machine that could be used to produce tryptophan</span>

                        </div>

                        <div class="imgWrapper centerize">

                            <img src="https://static.igem.org/mediawiki/2021/e/e6/T--XJTU-China--16-Chart-6-New.png"

                                alt="Fig. 6" width="90%">

                            <span class="description"><strong>Fig.6</strong> Main concerns for people to consider when

                                they choose these kind of machine</span>

                        </div>

                <p>According to the survey, if the technologies are mature enough, nearly 95% of the people choose to

                    accept and show willingness to purchase and try the household production instruments. The three most

                    crucial aspects of instrument design are safety, price and function, accounting for 66.63%, 57.78%

                    and 45.33% respectively, which provides us with a direction for designing our instruments in a more

                    custom-oriented way. And after the analysis of Pearson correlation, we can safely conclude that

                    there is a significant positive correlation between accepting household instruments and eating to

                    regulate sleep and receiving microbial fermentation products to help sleep, indicating that using

                    household instruments to ferment tryptophan production for the treatment of insomnia has a wide

                    audience . ( For details of the analysis results, please refer to the "Relationship between Diet to

                    Sleep, Microbial Fermentation Attitude and Receiving Instruments")</p>

                <br>

                <p>It was worth mentioning that we were glad to take an active part in many meetups dyring each of our

                    stage. In the preparation stage, we joined the meetup held by NWU-China-A to share our idea about the

                    program we were going to do. During the implementation stage we took part in the meetup held by JNU and

                    the national meetup: CCIC, both of the meetings enabled us to share our experience and exchange ideas

                    about the problems we met.</p>

                <a class="anchor" id="2"></a>

                <h2 class="mt-5" >Further publicity</h2>

                <p>Our effort absolutely not limited to the project itself because we endeavor to make more positive influence on the nowadays society. This year, we focus on mental problems like anxiety, insomnia and depression and basic knowledge about nutriology. As a consequence, diverse types of activities are planned and carried out to impart healthy lifestyle to people, which we think is more important than just doing the project itself. Lectures towards different age of students, debate competition, brochure, hands-on experience, all of these let us find more possibilities to enlarge the meaning of our project and we are always eager to try these new things. </p>

                <a class="anchor" id="2-1"></a>

                <h3 class="mt-5" >1. Lecture for High School Students about Synthetic Biology and iGEM</h3>

                <div class="row">

                    <div class="col-5">

                        <p>It’s our expectation to enhance the public’s comprehension of synthesis biology, therefore,

                            <b>we sought an appreciable opportunity to organize a lecture about synthesis biology and

                                its technologies for high school students.</b> More than demonstrating the basic theories and

                            technologies of bioscience, during the lecture, we’d like to discuss how bioscience had

                            contributed to the development of the society.</p>

                        <br><br>

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                                alt="Fig. 3" width="90%">

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                            <img src="https://static.igem.org/mediawiki/2021/0/0b/T--XJTU-China--Education-Fig2.png"

                                alt="Fig. 2" width="90%">

                <a class="anchor" id="2-2"></a>

                <h3 class="mt-5" >2. Summer Camp Activities——Go to Laboratory</h3>

                <p>We are dedicated to introducing bioscience to kids and cultivating their active attitude towards

                    science and their interest to exploring the nature. Therefore, we decided to organize summer camps

                    activities for high school and elementary school students.</p>

                <div class="row">

                    <div class="col-6">

                        <div class="imgWrapper centerize">

                            <img src="https://static.igem.org/mediawiki/2021/9/9c/T--XJTU-China--Education-Fig4.png"

                                alt="Fig. 4" width="90%">

                         <br><br>

                        <p>At the same time, we also taught high school students to <b>do an experiment of painting

                            with fluorescent E.coli</b> to get them directly fascinated by the charm of experiment,

                            through which they would gain not only technique, but also the comprehension of the

                            beauty of bioscience.</p>

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                            <img src="https://static.igem.org/mediawiki/2021/e/ee/T--XJTU-China--Education-Fig6.png"

                                alt="Fig. 6" width="90%">

                     <div class="col-6">

                        <p>During the summer camp, we guided them into advanced laboratories of synthesis biology in

                            order to illustrate synthesis biology and its real-word applications. While visiting the

                            lab, we introduced them the <b>standards of operation in the lab and its significance</b>, as well

                            explore the joy of ameliorating the world with their own hands.</p>

                         <div class="imgWrapper centerize">

                            <img src="https://static.igem.org/mediawiki/2021/2/2e/T--XJTU-China--Education-Fig5.png"

                                alt="Fig. 5" width="90%">

                         <br>

                        <p>Moreover, we organized a mini-brainstorm to guide them to consider <b>how to make use of

                             biotechnology to bring benefits to the society.</b> They were quite immersed into the activity,

                            coming up with a plenty of brilliant ideas, for instance, utilizing good germs to kill bad

                            germs to cure inflammations and simulating the circumstance inside the gut to produce Dog

                            Coffee, an analog to Cat Coffee. We expect to stimulate their active thought and their

                            interest to synthesis biology, and ultimately, guiding them to be the next generation of

                <a class="anchor" id="2-3"></a>

                <p>It is a good way to attract the general public’s interest by carrying out novel popular science

                    activities about hot issues. Considering the needs of effective knowledge spread, interesting

                    science popularization, thought-provoking discussion topics and so on, we conducted a debate

                    competition with the theme of <b>"Is gene editing a good news or disaster for mankind?"</b> for

                    college students. </p>

                <br>

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                            <img src="https://static.igem.org/mediawiki/2021/a/a1/T--XJTU-China--Education-Fig7.png"

                                alt="Fig. 7" width="90%">

                     <div class="col-6">

                        <p>During the race, debaters showed audiences plenty of details about gene editing technical,

                            laboratory research and commercial conditions, advantages and possible risks over

                            traditional biotechnology, and also ethical issues that cause public concerns. We planned

                            to help the audiences to view gene editing and other techniques in a more scientific and

                            holistic way, at the same time, help them understand the benefits and potential risks of

                            synthetic biology.</p>

                        <br>

                         <div class="imgWrapper centerize">

                            <img src="https://static.igem.org/mediawiki/2021/a/a7/T--XJTU-China--Education-Fig8.png"

                                alt="Fig. 8" width="90%">

                    <div class="col-6">

                        <div class="imgWrapper centerize">

                            <img src="https://static.igem.org/mediawiki/2021/2/28/T--XJTU-China--Education-Fig9.png"

                                alt="Fig. 9" width="90%">

                     <div class="col-6">

                        <div class="imgWrapper centerize">

                            <img src="https://static.igem.org/mediawiki/2021/2/28/T--XJTU-China--Education-Fig10.png"

                                alt="Fig. 10" width="90%">

                         science and bring the idea of healthy life to the public.</b> And according to the review with Pro.Zhang