Team:XJTU-China/Parts
Parts
In order to increase the yield of tryptophan in E.coli, the gene of key enzyme AroG which catalizes the synthesis of DAHP by condensation of PEP and E4P, is selected to be overexpressed. To maximum the output, a mutant AroG (S211F) without Phe feedback inhibition is picked to construct the induced expression system. And this part is documented under BBa_K3832008. In addition, a toggle switch circuit with GFP and RFP is also constructed. It can achieve bistable status and switch in certain condition, like IPTG induction and 42℃ heat, and the expression of reporter mRFP and sfGFP is upregulated, resulting in toggle of fluorescence. This part is documented under BBa_K3832007. Trp-indece is a composite part that detects the cellular trptophan level. As the concentration of And all other new basic parts included in our project are listed in the table.
| # | Name | Type | Description | Length |
|---|---|---|---|---|
| BBa_K3832000 | aroG (Mutant S211F) | Coding | Phospho-2-dehydro-3-deoxyheptonate aldolase (mutant S211F) | 1053bp |
| BBa_K3832003 | Medium RBS (RBS-B) | RBS | A medium RBS in E.coli | 10bp |
| BBa_K3832004 | Medium RBS (RBS-D) | RBS | A medium RBS in E.coli | 14bp |
| BBa_K3832005 | Weak RBS (RBS-H) | RBS | A weak RBS in E.coli | 11bp |
| BBa_K3832007 | Toggle-switch circuit with GFP and RFP | Composite | A bistable toggle switch with two promoter-repressor systems (lacUV5 promoter-LacI and lambda-CI857) and reporter genes (sfGFP and mRFP) | 3685bp |
| BBa_K3832008 | Induced expression circuit of aroG (Mutant S211F) | Composite | An expression system of aroG-S211F induced by IPTG | 2503bp |
| BBa_K3832009 | Trp-induce | Composite | The detection cirtuit of tryptophan | 2114bp |
| BBa_K3832010 | pykA | CDS | E.coli pyruvate kinase II | 1443bp |
| BBa_K3832011 | trpBA | CDS | Two adjacent genes in trp gene cluster, encoding the beta chain and alpha chain of tryptophan synthase, respectively. | 2000bp |