Notebook
July | |
4th | Prepare culture mediums. |
5th | Amplify the plasmid fragments by PCR, gel electrophoresis and recycle DNA. |
6th | Construction of the 4 plasmids. |
7th | Colony PCR, gel electrophoresis, and select single clones. |
8th | Plasmids extraction from E. Coli, and sent to the DNA sequencing company. |
9th | Verify the DNA sequencing resuls and carry out the transformation of plasmids into the Saccharomyces cerevisiae by Heat-Shock approach. |
11st | Prepare competent cells of the Saccharomyces cerevisiae, and incubate the yeast single colony in 1ml culture mediums. |
12nd | Day off. Due to the limited time of incubation, our Saccharomyces cerevisiae didn't demonstrate efficient growth. Hence, we were not able to carry out the enzyme acitivety tests. |
13th | Carry out fermentation performance test by testing the concentrations of glucose and ethanol. |
August | |
Repeat the plasmids construction experiments and the yeast culture by AQ yeast. | |
September | |
Carry out enzyme activity tests and fermentation performance tests. |