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                    <h3>Integrated HP</h3>

                    <ul>

                        <li><a class="fa fa-plug" href="#inspiration">&nbsp;Content</a>

                                <li><a href="#inspiration"><b>· Inspiration & Investigation</b></a></li>

                                     <!--                                        <li><a href="#overview_2">&emsp;- Overview</a></li>-->

                                    <li><a href="#point_1">&emsp;- I. Interview with Prof.Zhang</a></li>

                                    <li><a href="#point_2">&emsp;- II. Interview with Dr.Chen</a></li>

                                <li><a href="#implementation"><b>· Implementation</b></a></li>

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                <h1 class="mt-5">Content</h1>

                <a class="anchor" id="inspiration"></a>

                <h2 class="mt-5">Inspiration and background investigation</h2>

                <a class="anchor" id="overview_2"></a>

                <p><b>Overview:</b> In the preparation stage of the project, we carried out a wide range

                    of background investigations to confirm the feasibility of using tryptophan to release the pain

                    of

                    insomnia. We interviewed with Prof.Zhang Yali, in order to find out the possible ways of using

                    tryptophan to cure insomnia. After the deep interaction with Prof.Zhang, we decided to explore

                    the possibility of using milk as medium to help people absorb extra tryptophan. And to make the

                    step further, considering the background of epidemic, the idea of constructing a domestic milk

                    machine to fulfill our goal attracted our attention successfully. Meanwhile, we were honored to

                    have a deep communication with Dr. Chen Xiaopeng, who had much experience in curing patients

                    with

                    mental health problems like sleep disorder. The talk covered a lot of useful contents: from the

                    current situation and therapies of insomnia, to the feasibility of using tryptophan to cure

                    insomnia. Dr. Chen even inspired us to do some popularization of science to introduce the

                    insomnia

                    and its solutions towards public.</p>

                <a class="anchor" id="point_1"></a>

                <h3 class="mt-5">I. Interview with Prof.Zhang Yali</h3>

                <div align="center"><img src="https://static.igem.org/mediawiki/2021/6/64/T--XJTU-China--2-ZYL-Photo.png"

                                        alt="Pohto of Prof. Zhang" width="20%"></div>

                <p>

                    In order to understand the anxiety problems and sleep conditions in the context of the epidemic

                    and

                    to learn to choose the suitable medium to carry our tryptophan, we interviewed Prof. Zhang Yali

                    from

                    the School of Life Science of Xi'an Jiaotong University.</p>

                <p>Prof. Zhang introduced us the interdisciplinary intersection of microbiology and food nutrition

                    with

                    the fermentation industry as the core, affirming the broad prospects of microbiology and

                    synthetic

                    biology.She mentioned that under the social background of epidemic, making difference to

                    people’s

                    diet is more safer and convenient. Pro. Zhang alos discussed with us the selection of

                    engineering

                    bacteria and explore the value of tryptophan in various applications.</p>

                <p>At the same time, we were also glad to know that tryptophan has much wider application in

                    diverse area than we used to think, which can be used to help people release their feeling of

                    anxiety and depression. And further investigations proved this idea.</p>

                <p>With the guidance and suggestions, we chose to use food medium to carry our tryptophan, and would

                    focus on the issues of product acceptance and safety came up with idea to use milk reserved in a

                    domestic.</p>

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                        src="https://static.igem.org/mediawiki/2021/7/7f/T--XJTU-China--3-ZYL-Interview.png"

                        alt="Interview with Prof. Zhang" width="90%"></div>

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                    <p>Under the circumstance of recurring epidemic, mental health problems caused by reduced

                        socialization,

                        like increased anxiety and widespread sleep problems receive much attention from the public.

                        And due

                        to our goal of helping people solve these problems by making difference to their diet, we

                        actually

                        need the interaction with expert in the area of nutrition and food health. Luckily , our

                        team had a

                        chance to interview Pro. Zhang Yali from the School of Life Sciences at Xi'an Jiaotong

                        University,

                        who has profound knowledge of nutriology. We realized that we can meet human health needs

                        with the

                        help of food nutrition and microbiology, while gaining a deeper understanding of our

                        tryptophan

                        project.</p>

                    <p>Prof. Zhang has long been engaged in microbiology and food nutrition research, so she began

                        by

                        introducing us the most central part of the intersection of the two fields: the fermentation

                        industry supported by the microbiology discipline. For the purpose of producing natural

                        products

                        using genetic engineering techniques, there are both advantages and disadvantages compared

                        to the

                        direct use of chemical synthesis. At present, there are more than 80 polypeptide drugs

                        available

                        worldwide, which are obtained by three methods: chemical synthesis, genetic recombination

                        and plant

                        or animal extraction. There are still extreme challenges in the process of developing new

                        polypeptide drugs. Meanwhile, the strict regulations to their application are supposed to be

                        considered.</p>

                    <p>In the post-epidemic era, individuals are paying more attention to their own health

                        conditions.

                        Prof. Zhang believed that this trend provides a broad prospect for the development of

                        microbiology

                        and synthetic biology, in order to meet the needs of the public. And microorganisms have

                        pivotal

                        impact on human health. First of all , microorganisms have coexisted with humans for a long

                        time in

                        our intestinal, oral and skin flora, which have a long-lasting and stable impact on human

                        homeostasis and immunity. On top of that, microorganisms can also affect human indirectly by

                        influencing our living environment. Therefore, the research in the direction of the human

                        microbiome

                        is also crucial to be further explored.</p>

                    <p>Finally, we and Prof.Zhang focus on the design and idea of our project. In terms of hardware

                        design,

                        Prof. Zhang suggested us to grasp the selection of engineered bacteria to ensure the sensory

                        properties of the product and its curative effect are accepted. At the same time,

                        tryptophan, as a

                        precursor substance ,may not be as effective as melatonin in the short term. But as a

                        component of

                        food and more importantly, the essential amino acids for human life, its longevity and

                        organism

                        stability are much better. Moreover, tryptophan is involved in numerous metabolic processes,

                        and the

                        injection of it is of great clinical value in treating diseases like hypertension and

                        depression.

                        Combined with iron and vitamins, tryptophan can intervene in locomotor anemia; the

                        combination with

                        histidine can treat peptic ulcers. Therefore, the prospect for the application and

                        development of

                        tryptophan is really wide.</p>

                    <p>With the guidance and advice of Prof. Zhang, we learned that tryptophan has a broad

                        application

                        prospect, at the same time,we needed to focus on product acceptance and safety issues in

                        hardware

                        development.</p>

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                <a class="anchor" id="point_2"></a>

                <h3 class="mt-5">II. Interview with Dr.Chen Xiaopeng</h3>

                <p>In order to gain insight into the causes and treatment of insomnia today and explore new ideas

                    for

                    sleeping aid, our team contacted and interviewed Dr. Chen Xiaopeng, Deputy Chief Physician of

                         clinical application of tryptophan is controversial, a recent Meta-analysis by the National

                        University of Singapore on the effect of tryptophan on sleep disorders indicated that

                        adequate

                        supplementation of tryptophan can significantly reduce the time to fall asleep. As a

                        consequence,

                        the direction of our project is worthy of trying and exploring.</p>

                    <p>Finally, Dr.Chen offered some suggestions to prevent insomnia for today’s youth. To start

                        with

                        subjective causes, many surveys have shown that common triggers of insomnia among students

                        include excessive use of electronic products such as cell phones, smoking, drinking alcohol

                        or milk

                        tea, learning pressure and emotional instability. Therefore, Dr. Chen suggested students to

                        pay

                        attention to overcoming these factors in their lives. In addition, scientific research also

                        indicates that melatonin secretion in the human body starts at about 9:00 pm and gradually

                        decreases to stop after about 12:00 pm. The gastrointestinal motility of the digestive

                        system stops

                        at about 10:30 pm, which reminds us that we should arrange the pace of life reasonably.</p>

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                <h2 class="mt-5">Implementation</h2>

                <a class="anchor" id="overview_3"></a>

                <p><b>Overview:</b> When it came to the implementation stage, the talk with Prof.Xu Dan provided us with lots of precious suggestions in designing and carrying out our wet lab experience. During the process of  experience, we came across lots of problems that we were not able to deal with. As a consequence, we consulted our instructors or advisors frequently to find ways to solve these obstacles effectively and pushed the project forward. And it absolutely included Dr. Liu Ziqiang, who has engaged in the area of tryptophan fermentation for almost seven years. He offered us not only the information of cutting-edge progress made in tryptophan fermentation, but also some essential technical suggestions to polish our wet lab design, like the choice of model organism and resistance, the optimization of fermentation conditions and the purification of tryptophan to make it as clean as possible.</p>

                <a class="anchor" id="point_3"></a>

                <h3 class="mt-5">III. Interview with Prof.Xu Dan</h3>

                <div align="center"><img src="https://static.igem.org/mediawiki/2021/8/86/T--XJTU-China--4-XD-Photo.png"

                                        alt="picture of Prof. Xu" width="20%"></div>

                <p>We conducted a communication in HP with NWU-CHINA-A team, and jointly interviewed Associate

                    <p>Our team had a communication with NWU-CHINA-A team about HP. And we interviewed Associate

                         Xu Dan from Xi'an Jiaotong University. Prof. Xu has specialized in the field of microbiology

                        many years, and during the cooperation, we clarified the promising application of bacterium

                    <p>First of all, Professor Xu introduced us a problem in synthetic biology projects nowadays:

                        exogenous genes can be successfully expressed in host microorganisms. The main reasons exist

                         application of synthetic biology in achieving industrial mass production. Although

                        industrial mass

                        production needs to take factors like gene component assembly, splicing and expression

                        product

                        selection, expression efficiency evaluation, expression product purification, etc into

                        consideration, it is a topic with great potential and investment value. So, it is worthy of

                        time and

                        energy we spared to learn and master. Finally, Prof. Xu stressed that synthetic biology is a

                        field

                        with lots of "down-to-earth" inventions that will benefit our life in a comprehensive way.

                        And in

                        the future, the achievement in synthetic biology will continue to gain popularity and

                        attention in

                        real life for its convenience and wide-spread use.</p>

                    <p>Through the interview with Prof. Xu, we have a better understanding of the field of synthetic

                        biology. In our project, we will continue to focus on the purification of synthetic biology

                        products

                        and the yield issues.</p>

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                <h3 class="mt-5">IV. Interview with Dr.Liu Ziqiang</h3>

                <div align="center"><img

                        src="https://static.igem.org/mediawiki/2021/4/40/T--XJTU-China--17-LZQ-Photo.jpeg"

                        alt="Photo of Dr. Liu" width="25%"></div>

                <p>In order to solve the problems concerning fermentation device design and learn more about

                    tryptophan

                    production, we consulted Dr. Liu. </p>

                <p>Dr. Liu eagerly shared with us the information about the specific experimental process, some

                    notices

                    in fermentation process and the possibility of using periodic bacteria package, with his own

                    experience in the research of fermentation of tryptophan. On the premise of ensuring the high

                    quality and continuous production of tryptophan, Dr. Liu gave us some suggestions on the design

                         separation process. Secondly, in the design of fermentation device, Dr. Liu suggested

                        considering

                        the compatibility of function and volume. However, the current problem is that the material

                        transport between different accessories in the device mainly relies on pressure differential

                        method and pumps, so it’s hard to achieve the goal of “both small and multi-functional”.

                        Apart from that, striking a balance between costs and consumers is quite hard. If high

                        techniques

                        are used, it undoubtedly leads to increasing price, which is hard to accept by customers.

                    </p>

                    <p>Finally, he talked about the present situation of tryptophan production. Amino acid products,

                        as a

                        food additive, have been widely used in the field of food and health care with huge demand

                        and

                        market. Specifically, the maximum annual production of the company with mature tryptophan

                        fermentation technology is about 10,000 tons. However, after purification, there is a 20% to

                        30%

                        loss in tryptophan fermentation liquid, which leads the total conversion rate stay at about

                        10%, far

                        less than that of glutamate (60-70%). The key to improve tryptophan fermentation technology

                        is to

                        improve strains we use. Selecting more appropriate strains can largely improve conversion

                        rates,

                        releasing burden of purifying.</p>

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                <a class="anchor" id="application"></a>

                <h2 class="mt-5">Application</h2>

                <a class="anchor" id="overview_4"></a>

                <p><b>Overview:</b>In the application stage, we tried our best to broaden the prospect

                    of our project. After the talk with Dr. Liu Ziqiang, we decided to try to pay a visit to

                    factory. And finally, we contacted with a alcohol fermentation factory to get some experience of

                    bacteria fermentation. With the original design of our hardware, we made connection with

                    that, he eagerly gave us much advice to help us make our design more reliable and user-friendly.

                    He also mentioned that it would be better for us to polish our hardware if we can carry out

                    survey to collect first-hand data. As a result, we did a public survey to evaluated the current

                    situation of insomnia, as well as the acceptability of our hardware design. The outcome of the

                    survey showed that public awareness of insomnia needs to be strengthen. And surprisingly, nearly

                    of investigated population held an open mind to our hardware and were willing to try it if the

                    technology is mature enough.</p>

                <a class="anchor" id="point_5"></a>

                <h3 class="mt-5">V. Interview with Dr.Zong Yeqing</h3>

                <div align="center"><img src="https://static.igem.org/mediawiki/2021/e/ee/T--XJTU-China--6-ZYQ-Photo.png"

                                        alt="Picture of Prof. Zong" width="20%"></div>

                <p>In order to gain an in-depth understanding of the hardware development later in the project, we

                    discussed our hardware design with Dr. Zong from BluePha.</p>

                <p>To start with, we discussed the feasibility of achieving what we had already designed with Mr.

                    Zong,

                    and asked Mr. Zong how to choose the form of final product (hardware). He pointed out some key

                    factors we need to consider during our design process, like whether our hardware is

                    user-friendly

                    and cost-efficient. Some obstacles in technologies are hard for us to overcome, which made us to

                    polish the design of our machine. Also, we gained the suggestions for our Human Practice, to

                    help us

                    gain the attitude of public toward our design.</p>

                <p>Through discussions with Mr. Zong, we have a deeper understanding of the hardware design and

                    development, which is of great importance in home-based hardware design. And we also planned to

                    carry out a survey relayed to our project to collect more ideas and possibilities.</p>

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                    <p>In order to gain an in-depth understanding of the hardware development later in the project,

                        we

                        interviewed Dr. Zong from Bluepha Lab and discussed our design ideas for creating a hardware

                        of

                        tryptophan producing. First of all, Dr. Zong gave his advice on the feasibility of

                        fermenting drinks

                        through portable device and then extracting tryptophan to make drinks. He explained that

                        bio-fermentation is still a main source of producing tryptophan, so what we need to focus

                        now is

                        selecting strains with high productivity, which subsequently leads to the maximum tryptophan

                        yield.</p>

                    <p>In addition, Dr. Zong taught us the reasonable methods of analyzing the feasibility of our

                        hardware.

                        First, we need to know the highest concentration of tryptophan at present, and then design

                        strains

                        and systems based on it. Next, the hardware should be designed from this point of view.And

                        finally,

                        we should analyze the results and causes. Secondly, Dr. Zong talked about the need to design

                        hardware products from the angle of customers, as far as possible to meet the needs of

                        miniaturization and simplicity, that’s what we call user-friendly design. Also, designing

                        the

                        hardware originally, being innovative and making improvement based on the previous version

                        are

                        vital.</p>

                    <p>Surprisingly, Dr. Zong also gave us a few suggestions for doing HP. First, members in Hp

                        group needs

                        to demonstrate the importance of our design for everyday life and industrial production. The

                        second

                        suggestion is adding more field trips into HP activities, which can help us accumulate

                        enough data

                        and increase the credibility of our design. In short, through the conversation with Dr.

                        Zong, we had

                        a deeper understanding of the design and development of hardware, which is of great

                        importance in

                        home-based hardware design.</p>

                </div>

                <div id="morebutton5">

                    <a onclick="readmore5()">

                        <p><b>[CLICK TO READ MORE]</b></p>

                    </a>

                </div>

                <a class="anchor" id="point_6"></a>

                <h3 class="mt-5">VI. Visit to the Shaanxi Qinyang Changsheng Liquor Factory</h3>

                <div align="center"><img src="https://static.igem.org/mediawiki/2021/4/49/T--XJTU-China--8-Visit-1.png"

                                        alt="Visit Photo 1" width="90%"></div>

                <p>In order to address the issues during the fermentation of tryptophan including bacterial

                    contamination, strain passage and product purification, we decided to pay a visit to the related

                    factories. But the restriction of epidemic had a great impact on our plan. After contacting for

                    a

                    long time and considering the possibility, we went on a field trip to tour the Shaanxi Qinyang

                    Changsheng Liquor Co., Ltd, to find the similar methods we may apply in our projects. </p>

                <p>We interviewed Mr. Wang Ping and Mr. Wu Dongping there, and drew on their experience concerning

                    technology used in industrial brewing process.</p>

                <div align="center"><img src="https://static.igem.org/mediawiki/2021/5/5b/T--XJTU-China--9-Visit-2.png"

                                        alt="Visit Photo 2" width="90%"></div>

                <p>First of all, we visited the whole production line with the staff’s guide. Mr. Wang and Mr. Wu

                    pointed out that the qualified personnel, the control of technological skills and equipment

                    sanitization are the requisites for contamination avoidance. In addition, since fermentation is

                    literally a process with complex mixtures of various microbes, it is not realistic to circumvent

                    all

                    the pollutant. We also gained insight into the separation techniques and the way to maintain

                    microbiological stable (by controlling the key strains in different techniques). Furthermore, to

                    ensure the quality of fermented products, it is vital to create a condition (temperature,

                    humidity

                    etc.) suitable for microbes.</p>

                <div align="center"><img

                        src="https://static.igem.org/mediawiki/2021/9/99/T--XJTU-China--10-Visit-Interview.png"

                        alt="Visit Interview" width="90%"></div>

                <p>Mr. Wang and Mr. Wu also pointed out that one of the main research directions in this filed is

                    removing the mycotoxin, which is potentially responsible for contamination out of the

                    fermentation

                    system, though Qinyang Changsheng Liquor Co., Ltd doesn’t have a process relating to it.</p>

                <a class="anchor" id="point_7"></a>

                <h3 class="mt-5">VII. A survey about insomnia and public’s attitude toward our design of

                    hardware</h3>

                <p>A survey was carried out to understand the sleep status, problems and people’s attitudes towards

                    microbial fermentation of producing sleep-aid products and home production instruments. The

                    respondents we surveyed were all ages, with a total of 1,020. </p>

                <p>Obviously, the information we collected from the public actively polish our design ideas and

                    reveal

                    the potential obstacles we may face when we try to make our design down to earth. What’s more,

                    the

                    result also helps us to precisely evaluate the market need for our hardware, in order to make

                    our

                    project go further.</p>

                <p><b>The result about sleep quality of people shows two problems</b></p>

                <p>1. More than half investigated individuals are suffered from sleep problems:</p>

                <div class="row mt-5">

                    <div class="col-6">

                        <div class="imgWrapper centerize">

                            <img src="https://static.igem.org/mediawiki/2021/c/c1/T--XJTU-China--11-Chart-1-Newest.png"

                                alt="Fig. 1" width="80%">

                            <span class="description"><strong>Fig.1</strong> Whether people had problems about their

                    <div class="col-6">

                        <div class="imgWrapper centerize">

                            <img src="https://static.igem.org/mediawiki/2021/b/ba/T--XJTU-China--12-Chart-2-New.png"

                                alt="Fig. 2" width="80%">

                            <span class="description"><strong>Fig.2</strong> the sleep loss frequency for people who

                <p>More than 60% of the respondents have insomnia in the past month, more than 60% of them suffered

                    from

                    insomnia over 3 times in a month, and more than 20% of them suffered from insomnia over 9 times

                    in a

                    month. Long time of falling asleep, poor sleep quality and short sleep time are common problems

                    for

                    insomniacs. And according to Pearson-related analysis, sleep status is not related to age or

                    gender,

                    which means that people of all ages suffer from insomnia. (The results of the analysis are

                    detailed

                    in "Relevance between gender, age and sleep status")</p>

                <p>2. The traditional treatment of sleep problems has achieved little success:</p>

                <div class="imgWrapper centerize">

                    <img src="https://static.igem.org/mediawiki/2021/b/be/T--XJTU-China--13-Chart-3-New.png" alt="Fig. 3"

                        width="90%">

                    <span class="description"><strong>Fig.3</strong> Different methods to deal with insomnia and

                </div>

                <p>Although a great deal of people suffer from insomnia, only 17.97% of these people have tried to

                    treat

                    it. And the survey results show that hospital visits, drug sleep aids, physical sleep aids, and

                    popular sleep aids account for only about 15% on average. It can be seen that most insomniacs'

                    sleep

                    problems have not improved after treatment.</p>

                <p><b>The result about microorganism fermentation show two results</b></p>

                <div class="imgWrapper centerize">

                    <img src="https://static.igem.org/mediawiki/2021/6/6c/T--XJTU-China--14-Chart-4-2-Newest.jpeg"

                        alt="Fig. 4" width="90%">

                    <span class="description"><strong>Fig.4</strong> People’s understanding of microorganism and

                </div>

                <p>1. People know little about microbial fermentation of producing drugs</p>

                <p>According to the surveys, only 17.63% of people have a certain understanding of Escherichia

                    coli's

                    ability to produce drugs, and only 9.86% have a certain understanding that tryptophan has

                    sleep-assisting effect. According to the Pearson-related analysis, there is a positive

                    correlation

                    between the acceptance of sleep aid products of microbial fermentation products and the

                    understanding of Escherichia coli and tryptophan (see "Acceptance of microbial fermentation and

                    understanding of bacteria and tryptophan for details of the analysis results).And the acceptance

                    rate is also positively related to age and the attempt to treat insomnia (analysis results are

                    detailed in "Analysis of Microbial Product Acceptance and Age). It shows that more knowledge

                    about

                    microbial fermentation drugs and insomnia treatment needs to be popularized to people to improve

                    acceptance.</p>

                <p>2. There is a broad prospect for Tryptophan produced by fermentation in aiding sleep: </p>

                <p>According to the survey results, 61.74% of the people have tried to solve insomnia through

                    dietary

                    therapy, and about 90% of people are reserved and acceptable in the face of sleeping-aid food

                    produced by microbial fermentation. It can be seen that most people may accept food containing

                    fermented tryptophan to improve sleep. Producing Tryptophan by fermentation within a domestic

                    machine is worth trying and will have its own audiences</p>

                <p><b>The investigation about hardware show a tendency</b></p>

                <p>1. High acceptance of domestic machine to produce Tryptophan: </p>

                <div class="imgWrapper centerize">

                    <img src="https://static.igem.org/mediawiki/2021/9/92/T--XJTU-China--15-Chart-5-New.png" alt="Fig. 5"

                        width="90%">

                    <span class="description"><strong>Fig.5</strong> People’s attitude toward the domestic

                </div>

                <div class="imgWrapper centerize">

                    <img src="https://static.igem.org/mediawiki/2021/e/e6/T--XJTU-China--16-Chart-6-New.png" alt="Fig. 6"

                        width="90%">

                    <span class="description"><strong>Fig.6</strong> Main concerns for people to consider when

                </div>

                <p>According to the survey, if the technologies are mature enough, nearly 95% of the people choose

                    to

                    accept and show willingness to purchase and try the household production instruments. The three

                    most

                    crucial aspects of instrument design are safety, price and function, accounting for 66.63%,

                    57.78%

                    and 45.33% respectively, which provides us with a direction for designing our instruments in a

                    more

                    custom-oriented way. And after the analysis of Pearson correlation, we can safely conclude that

                    there is a significant positive correlation between accepting household instruments and eating

                    to

                    regulate sleep and receiving microbial fermentation products to help sleep, indicating that

                    using

                    household instruments to ferment tryptophan production for the treatment of insomnia has a wide

                    audience . ( For details of the analysis results, please refer to the "Relationship between Diet

                    to

                    Sleep, Microbial Fermentation Attitude and Receiving Instruments")</p>

                <br>

                <p>It was worth mentioning that we were glad to take an active part in many meetups dyring each of

                    our

                    stage. In the preparation stage, we joined the meetup held by NWU-China-A to share our idea

                    about the

                    program we were going to do. During the implementation stage we took part in the meetup held by

                    JNU and

                    the national meetup: CCIC, both of the meetings enabled us to share our experience and exchange

                    ideas

                    about the problems we met.</p>

                <a class="anchor" id="2"></a>

                <h2 class="mt-5">Further publicity</h2>

                <p>Our effort were absolutely not limited to the project itself because we endeavor to make more positive

                    influence on the nowadays society. This year, we focus on mental problems like anxiety, insomnia

                    and depression and basic knowledge about nutriology. As a consequence, diverse types of

                    activities are planned and carried out to impart healthy lifestyle to people, which we think is

                    more important than just doing the project itself. Lectures towards different age of students,

                    debate competition, brochure, hands-on experience, all of these let us find more possibilities

                    to enlarge the meaning of our project and we are always eager to try these new things. </p>

                <a class="anchor" id="2-1"></a>

                <h3 class="mt-5">1. Lecture for High School Students about Synthetic Biology and iGEM</h3>

                <div class="row">

                    <div class="col-5">

                        <p>It’s our expectation to enhance the public’s comprehension of synthesis biology,

                            therefore,

                            <b>we sought an appreciable opportunity to organize a lecture about synthesis biology

                                and

                                its technologies for high school students.</b> More than demonstrating the basic

                            theories and

                            technologies of bioscience, during the lecture, we’d like to discuss how bioscience had

                            contributed to the development of the society.

                        </p>

                        <br><br>

                        <div class="imgWrapper centerize">

                            <img src="https://static.igem.org/mediawiki/2021/d/da/T--XJTU-China--Education-Fig3.png"

                                alt="Fig. 3" width="90%">

                    <div class="col-7">

                        <div class="imgWrapper centerize">

                            <img src="https://static.igem.org/mediawiki/2021/0/0b/T--XJTU-China--Education-Fig2.png"

                                alt="Fig. 2" width="90%">