Difference between revisions of "Team:Shanghai HS ID"

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{{Shanghai_HS_ID}}
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<h1> Welcome to iGEM 2021! </h1>
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<p>Your team has been approved and you are ready to start the iGEM season! </p>
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<h3>Before you start</h3>
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<p> Please read the following pages:</p>
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<li> <a href="https://2021.igem.org/Competition">Competition Hub</a> </li>
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<li> <a href="https://2021.igem.org/Competition/Deliverables/Wiki">Wiki Requirements page</a></li>
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<li> <a href="https://2021.igem.org/Resources/Template_Documentation">Template documentation</a></li>
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<h3> Styling your wiki </h3>
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<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
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<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>
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<h3> Uploading pictures and files </h3>
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<p> You must upload any pictures and files to the iGEM 2021 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. </p>
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<p>When you upload, set the "Destination Filename" to <b> T--YourOfficialTeamName--NameOfFile.jpg</b>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
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UPLOAD FILES
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<h3> Wiki template information </h3>
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<p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2021.igem.org/Judging/Pages_for_Awards">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
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<h3> Editing your wiki </h3>
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<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
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<p>Use WikiTools - Edit in the black menu bar to edit this page</p>
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<a href="https://2021.igem.org/wiki/index.php?title=Team:Shanghai_HS_ID&action=edit">
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EDIT PAGE
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<h3>Tips</h3>
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<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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<ul>
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<li>State your accomplishments! Tell people what you have achieved from the start. </li>
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<li>Be clear about what you are doing and how you plan to do this.</li>
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<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
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<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
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<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2021.igem.org/Calendar">iGEM 2021 calendar</a> </li>
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<li>Have lots of fun! </li>
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<h3>Inspiration</h3>
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<p> You can also view other team wikis for inspiration! Here are some examples:</p>
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<ul>
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<li> <a href="https://2019.igem.org/Team:Vilnius-Lithuania"> 2019 Vilnius Lithuania</a> </li>
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<li> <a href="https://2019.igem.org/Team:NUS_Singapore">2019 NUS Singapore</a> </li>
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<li> <a href="https://2019.igem.org/Team:Lambert_GA">2019 Lambert GA</a> </li>
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<li> <a href="https://2020.igem.org/Team:Waterloo"> 2020 Waterloo</a></li>
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<li> <a href="https://2020.igem.org/Team:TUDelft">2020 TUDelft</a></li>
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<li> <a href="https://2020.igem.org/Team:XMU-China"> 2020 XMU China </a></li>
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<li> <a href="https://2020.igem.org/Team:TAS_Taipei"> 2020 TAS Taipei </a></li>
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</ul>
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    <meta http-equiv="X-UA-Compatible" content="IE=edge">
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    <meta name="viewport" content="width=device-width, initial-scale=1.0">
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    <title>Shanghai_HS_ID</title>
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    <link rel="stylesheet"
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        href="https://2021.igem.org/wiki/index.php?title=Template:Shanghai_HS_ID/Main_CSS&action=raw&ctype=text/css" />
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    <link rel="stylesheet"
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        href="https://2021.igem.org/wiki/index.php?title=Template:Shanghai_United_HS/Swiper_CSS&action=raw&ctype=text/css">
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</head>
  
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<body>
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    <nav class="head-nav clearfix">
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        <div class="top-nav-bar">
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            <ul class="clearfix">
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                <li class="active">
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                    <a href="#">Home</a>
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                </li>
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                <li>
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                    <a href="">Project</a>
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                    <div class="sub-nav">
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                        <ul>
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                            <li><a href="https://2021.igem.org/Team:Shanghai_HS_ID/Description"
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                                    class="sub-nav-74">Description</a></li>
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                            <li><a href="https://2021.igem.org/Team:Shanghai_HS_ID/Experiments"
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                                    class="sub-nav-74">Experiments</a></li>
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                            <li><a href="https://2021.igem.org/Team:Shanghai_HS_ID/Results"
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                                    class="sub-nav-74">Results</a></li>
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                            <li><a href="https://2021.igem.org/Team:Shanghai_HS_ID/Proof_Of_Concept"
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                                    class="sub-nav-52">Proof Of
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                                    Concept</a></li>
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                            <li><a href="https://2021.igem.org/Team:Shanghai_HS_ID/Notebook"
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                                    class="sub-nav-52">Notebook</a>
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                            </li>
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                            <li><a href="https://2021.igem.org/Team:Shanghai_HS_ID/Safety">Safety</a></li>
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                        </ul>
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                    </div>
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                </li>
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                <li>
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                    <a href="">Parts</a>
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                    <div class="sub-nav">
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                        <ul>
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                            <li><a href="https://2021.igem.org/Team:Shanghai_HS_ID/Parts" class="sub-nav-74">Parts
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                                    Collection</a></li>
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                            <li><a href="https://2021.igem.org/Team:Shanghai_HS_ID/Engineering"
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                                    class="sub-nav-74">Engineering</a></li>
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                            <li><a href="https://2021.igem.org/Team:Shanghai_HS_ID/Contribution"
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                                    class="sub-nav-74">Contribution</a></li>
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                        </ul>
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                    </div>
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                </li>
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                <li>
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                    <a href="">Human Practices</a>
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                    <div class="sub-nav">
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                        <ul>
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                            <li><a href="https://2021.igem.org/Team:Shanghai_HS_ID/Human_Practices"
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                                    class="sub-nav-74">Integrated
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                                    Human Practice</a></li>
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                            <li><a href="https://2021.igem.org/Team:Shanghai_HS_ID/Communication"
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                                    class="sub-nav-74">Communication</a></li>
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                            <li><a href="https://2021.igem.org/Team:Shanghai_HS_ID/Fundraising"
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                                    class="sub-nav-74">Fundraising</a></li>
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                        </ul>
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                    </div>
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                </li>
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                <li>
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                    <a href="https://2021.igem.org/Team:Shanghai_HS_ID/Implementation">Implementation</a>
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                </li>
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                <li>
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                    <a href="https://2021.igem.org/Team:Shanghai_HS_ID/Entrepreneurship">Entrepreneurship</a>
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                </li>
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                <li>
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                    <a href="https://2021.igem.org/Team:Shanghai_HS_ID/Model">Model</a>
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                </li>
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                <li>
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                    <a href="">Team</a>
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                    <div class="sub-nav">
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                        <ul>
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                            <li><a href="https://2021.igem.org/Team:Shanghai_HS_ID/Members" class="sub-nav-74">Team
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                                    Members</a></li>
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                            <li><a href="https://2021.igem.org/Team:Shanghai_HS_ID/Attributions"
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                                    class="sub-nav-74">Attributions</a></li>
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                            <li><a href="https://2021.igem.org/Team:Shanghai_HS_ID/Collaborations"
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                                    class="sub-nav-74">Collaborations</a></li>
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                            <li><a href="https://2021.igem.org/Team:Shanghai_HS_ID/Partnership"
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                                    class="sub-nav-74">Partnership</a></li>
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                        </ul>
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                    </div>
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                </li>
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            </ul>
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        </div>
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    </nav>
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    <div class="home-content">
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        <div class="home-top-banner" style="margin-top: 25px;">
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            <img src="https://static.igem.org/mediawiki/2021/5/52/T--Shanghai_HS_ID--top.gif" />
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        </div>
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        <div class="swiper-container top-swiper-container group-photo">
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            <div class="swiper-wrapper">
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                <div class="swiper-slide">
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                    <img src="https://static.igem.org/mediawiki/2021/f/f9/T--Shanghai_HS_ID--homepage01.jpg" />
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                </div>
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                <div class="swiper-slide">
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                    <img src="https://static.igem.org/mediawiki/2021/a/ac/T--Shanghai_HS_ID--homepage02.jpg" />
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                </div>
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            </div>
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            <div class="swiper-pagination top-swiper-pagination"></div>
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        </div>
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        <div class="team-tips">
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            <p class="team-tips-title">Abstract</p>
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            <div class="team-tips-content">Lactobacillus casei was found to have the potential to cure and prevent
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                common health issues like high cholesterol, hypertension, and gastrointestinal diseases. Recent studies
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                show that L. casei's capabilities in medicine and food are highly restricted by their low transformation
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                efficiency of foreign DNA, due to its restriction-modification system. Our purpose is to improve the
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                transformation efficiency of foreign DNA in L. casei to maximize their benefits. In the laboratory, we
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                constructed a plasmid containing CRISPR-Cas9 in E.coli, then transformed the plasmid into L.casei so
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                that the target gene LSEI-2094 can be removed and the restriction-modification system can be silenced.
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                Lastly, we confirmed the results through both sequencing and controlled experiments to verify the
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                efficiency before and after the modification. Results show that the transformation efficiency has
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                significantly increased post-modification, it is hopeful that this modified L. casei could be applicable
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                in pharmaceutical and food. </div>
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            <p class="team-tips-title">Promotion video</p>
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            <div class="team-tips-video-wrap">
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                <video width="100%" height="670" controls class="home-video">
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                    <source src="https://static.igem.org/mediawiki/2021/c/cf/T--Shanghai_HS_ID--video.mp4" type="video/mp4">
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                </video>
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            </div>
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        </div>
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    <footer class="footer">
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        <section class="footer-wrap">
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            <div class="footer-contact">Follow us on WeChat!</div>
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            <img class="footer-qrcode" src="https://static.igem.org/mediawiki/2021/c/c3/T--Shanghai_HS_ID--qrcode.png" />
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            <p class="margin-bottom-10"><i>WeChat Official Account: 乳酸LAB</i></p>
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            <p>Contact Email：<i>jhxchen@gmail.com</i></p>
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        </section>
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    </footer>
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</body>
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<script
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    src="https://2021.igem.org/wiki/index.php?title=Template:Shanghai_United_HS/Swiper_JS&action=raw&ctype=text/javascript"> </script>
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<script>
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    let liTags = document.querySelectorAll(".top-nav-bar > ul > li");
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    for (let i = 0; i < len; i++) {
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        liTags[i].onclick = function (e) {
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            //先移除所有的点击样式
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                liTags[j].classList.remove("active");
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        speed: 2000,
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</script>
  
 
</html>
 
</html>

Latest revision as of 17:48, 19 October 2021

Shanghai_HS_ID

Abstract

Lactobacillus casei was found to have the potential to cure and prevent common health issues like high cholesterol, hypertension, and gastrointestinal diseases. Recent studies show that L. casei's capabilities in medicine and food are highly restricted by their low transformation efficiency of foreign DNA, due to its restriction-modification system. Our purpose is to improve the transformation efficiency of foreign DNA in L. casei to maximize their benefits. In the laboratory, we constructed a plasmid containing CRISPR-Cas9 in E.coli, then transformed the plasmid into L.casei so that the target gene LSEI-2094 can be removed and the restriction-modification system can be silenced. Lastly, we confirmed the results through both sequencing and controlled experiments to verify the efficiency before and after the modification. Results show that the transformation efficiency has significantly increased post-modification, it is hopeful that this modified L. casei could be applicable in pharmaceutical and food.

Promotion video

Follow us on WeChat!

WeChat Official Account: 乳酸LAB

Contact Email：jhxchen@gmail.com