Team:Shanghai HS ID/Notebook

Shanghai_HS_ID

NOTEBOOK

27^th June: We had our first team meeting and discussed our topic

28^th June: We made the work distribution and finalized the designs of the team name, logo, and merch. Besides, we conducted the interview with an expert.

Wet team agenda

28^th June:

• Lab safety training

30^th June- 2^nd July:

• Construction of sgRNA, upstream and downstream homologous arms
• Overlap PCR and electrophoresis
• Preparation of mediums
• Bacteria culture

3^rd July:

• pLCNICK enzyme digestion
• Connection of sgRNA and pLCNICK
• Transformation of the recombinant plasmid into E.coli

4^th July:

• Culture L. casei cells
• Preparation of competent L. casei cells

5^th July:

• Picked and shakes L. casei
• L. Casei PCR and electrophoresis

6^th-7^th July :

• Verification of the plasmid
• Electrotransformation of the plasmid into L. casei ATCC 533 August an September
• Repeat the plamid construction and electrotransformation
• L. casei cultivation

Dry team agenda

28^th June:

• Published the first article of our Wechat official account: introducing us and our topic.

30^th June:

• Discussed the script of our promotion video
• Listed survey questions and interview questions

1^st July :

• Organized and analyzed the records of the 1st Expert Interview
• Gathered information for our topic background
• Prepared a question list for the site visit to Bright Dairy & Food company in the next day
• Assigned tasks for the next day's site visit

2^nd July :

• Data analysis on survey results
• Integrated all interview records of the past few days
• Prepared questions for the 2nd Expert Interview
• Drafted a new article of our public Wechat account for the site visit
• Ordered merch: notebooks
• Designed post-it notes
• organize stuff for the article for the merch sale

3^rd July :

• Drafted self-introductions
• Drafted integrated human practices (transcription and translation of interview records, data of our Wechat official account and survey results)
• Booked another interview
• Listed interview questions
• Publish the second article of our Wechat official account, which was focused on our site visit to Bright Dairy & Food company.

4^th July :

• Drafted inspiration
• Discussed to determine the lecture location and target audience
• Promotion video filming
• Roll Screen design
• Visit BFC for merch-sale site pre-check

5^th July :

• Finalized IHP and inspiration
• Finalized details and publicize charity sale
• Started writing implementation and business plan
• Promotion video filming

6^th July :

• Drafted attributions, business plan
• Initially completed the script of our promotion video

7^th July :

• Planed for another charity sale
• Started writing a new article for merch sale to be published in our Wechat official account
• Collaboration activity
• Built our cost structure
• Finalized the script for promotion video
• First merch sale: yielded a profit of 50 RMB around and got 13 more followers

8^th July :

• Drafted future plan, safety, public engagement, and education
• Organized what we have done and drafted the first version of the presentation PowerPoint
• Fundraising

11^th July :

• Published the article about our merch sale in Wechat official account
• Determine the sale day online for our merch