NOTEBOOK
27th June: We had our first team meeting and discussed our topic
28th June: We made the work distribution and finalized the designs of
the team name, logo, and merch. Besides, we conducted the interview with an expert.
![](https://static.igem.org/mediawiki/2021/5/5f/T--Shanghai_HS_ID--Notebook01.jpg)
Wet team agenda
28th June:
• Lab safety training
![](https://static.igem.org/mediawiki/2021/2/2a/T--Shanghai_HS_ID--Notebook03.jpg)
30th June- 2nd July:
• Construction of sgRNA, upstream and downstream homologous arms
• Overlap PCR and electrophoresis
• Preparation of mediums
• Bacteria culture
• Overlap PCR and electrophoresis
• Preparation of mediums
• Bacteria culture
![](https://static.igem.org/mediawiki/2021/a/a9/T--Shanghai_HS_ID--Notebook04.jpg)
3rd July:
• pLCNICK enzyme digestion
• Connection of sgRNA and pLCNICK
• Transformation of the recombinant plasmid into E.coli
• Connection of sgRNA and pLCNICK
• Transformation of the recombinant plasmid into E.coli
![](https://static.igem.org/mediawiki/2021/6/66/T--Shanghai_HS_ID--Notebook05.jpg)
4th July:
• Culture L. casei cells
• Preparation of competent L. casei cells
• Preparation of competent L. casei cells
![](https://static.igem.org/mediawiki/2021/2/2a/T--Shanghai_HS_ID--Notebook06.jpg)
5th July:
• Picked and shakes L. casei
• L. Casei PCR and electrophoresis
• L. Casei PCR and electrophoresis
![](https://static.igem.org/mediawiki/2021/8/8d/T--Shanghai_HS_ID--Notebook08.jpg)
6th-7th July :
• Verification of the plasmid
• Electrotransformation of the plasmid into L. casei ATCC 533 August an September
• Repeat the plamid construction and electrotransformation
• L. casei cultivation
• Electrotransformation of the plasmid into L. casei ATCC 533 August an September
• Repeat the plamid construction and electrotransformation
• L. casei cultivation
![](https://static.igem.org/mediawiki/2021/7/74/T--Shanghai_HS_ID--Notebook09.jpg)
Dry team agenda
28th June:
• Published the first article of our Wechat official account: introducing us and
our topic.
![](https://static.igem.org/mediawiki/2021/d/d9/T--Shanghai_HS_ID--Notebook10.jpg)
30th June:
• Discussed the script of our promotion video
• Listed survey questions and interview questions
• Listed survey questions and interview questions
![](https://static.igem.org/mediawiki/2021/f/f4/T--Shanghai_HS_ID--Notebook11.jpg)
1st July :
• Organized and analyzed the records of the 1st Expert Interview
• Gathered information for our topic background
• Prepared a question list for the site visit to Bright Dairy & Food company in the next day
• Assigned tasks for the next day's site visit
• Gathered information for our topic background
• Prepared a question list for the site visit to Bright Dairy & Food company in the next day
• Assigned tasks for the next day's site visit
2nd July :
• Data analysis on survey results
• Integrated all interview records of the past few days
• Prepared questions for the 2nd Expert Interview
• Drafted a new article of our public Wechat account for the site visit
• Ordered merch: notebooks
• Designed post-it notes
• organize stuff for the article for the merch sale
• Integrated all interview records of the past few days
• Prepared questions for the 2nd Expert Interview
• Drafted a new article of our public Wechat account for the site visit
• Ordered merch: notebooks
• Designed post-it notes
• organize stuff for the article for the merch sale
![](https://static.igem.org/mediawiki/2021/f/f4/T--Shanghai_HS_ID--Notebook12.jpg)
3rd July :
• Drafted self-introductions
• Drafted integrated human practices (transcription and translation of interview records, data of our Wechat official account and survey results)
• Booked another interview
• Listed interview questions
• Publish the second article of our Wechat official account, which was focused on our site visit to Bright Dairy & Food company.
• Drafted integrated human practices (transcription and translation of interview records, data of our Wechat official account and survey results)
• Booked another interview
• Listed interview questions
• Publish the second article of our Wechat official account, which was focused on our site visit to Bright Dairy & Food company.
4th July :
• Drafted inspiration
• Discussed to determine the lecture location and target audience
• Promotion video filming
• Roll Screen design
• Visit BFC for merch-sale site pre-check
• Discussed to determine the lecture location and target audience
• Promotion video filming
• Roll Screen design
• Visit BFC for merch-sale site pre-check
![](https://static.igem.org/mediawiki/2021/4/4a/T--Shanghai_HS_ID--Notebook13.jpg)
5th July :
• Finalized IHP and inspiration
• Finalized details and publicize charity sale
• Started writing implementation and business plan
• Promotion video filming
• Finalized details and publicize charity sale
• Started writing implementation and business plan
• Promotion video filming
![](https://static.igem.org/mediawiki/2021/f/f0/T--Shanghai_HS_ID--Notebook14.jpg)
6th July :
• Drafted attributions, business plan
• Initially completed the script of our promotion video
• Initially completed the script of our promotion video
![](https://static.igem.org/mediawiki/2021/5/5b/T--Shanghai_HS_ID--Notebook15.jpg)
7th July :
• Planed for another charity sale
• Started writing a new article for merch sale to be published in our Wechat official account
• Collaboration activity
• Built our cost structure
• Finalized the script for promotion video
• First merch sale: yielded a profit of 50 RMB around and got 13 more followers
• Started writing a new article for merch sale to be published in our Wechat official account
• Collaboration activity
• Built our cost structure
• Finalized the script for promotion video
• First merch sale: yielded a profit of 50 RMB around and got 13 more followers
![](https://static.igem.org/mediawiki/2021/c/cd/T--Shanghai_HS_ID--Notebook16.jpg)
8th July :
• Drafted future plan, safety, public engagement, and education
• Organized what we have done and drafted the first version of the presentation PowerPoint
• Fundraising
• Organized what we have done and drafted the first version of the presentation PowerPoint
• Fundraising
![](https://static.igem.org/mediawiki/2021/c/c9/T--Shanghai_HS_ID--Notebook17.jpg)
11th July :
• Published the article about our merch sale in Wechat official account
• Determine the sale day online for our merch
• Determine the sale day online for our merch