Team:Shanghai HS ID/Safety
Safety
General Lab Safety
Our lab at ATLATL has the physical protection level of BSL-1, which means we only
deal with microorganisms with no pathogenicity to healthy adults.
Fire Safety
There are fire hydrants, fire extinguishers, automatic spray system, manual fire
alarm, fireproof shutter doors, and smoke alarms in the laboratory area. And the escaping route is
well-managed. So our lab meets the national fire safety regulations.
Individual Protection
Eating or drinking is not allowed in the lab area. Besides, white coats and PVC
gloves are required and only in the lab. Goggles are needed for hazardous experiments and an eye washer is
provided for unexpected accidents.
Emergency Handling
Once the laboratory encounters a dangerous or unmanageable situation (such as
experimental accidents, fires, leakage of dangerous goods, etc.), the person in charge of the laboratory
will arrange for the relevant team to deal with it immediately. This laboratory is equipped with trained
personnel to ensure a rapid response for any emergency.
Project Safety
Microorganism
We only used E. coli DH5α and Lactobacillus casei which are harmless to human
beings and all related experiments were conducted under the supervision of our instructor, Ms. Song.
The plasmids we used in the project are listed below:
pLCNICK(Addgene, #84653)
pIB165(Addgene, #90188)
pIB165(Addgene, #90188)
The primers we used in the project are listed below:
• Apal-334-2094-down-F：
CTAAGGATCACATCTTTTTCTAAACTAGGGCCCAACCAATCGTATCTTTGACAA
• BSAI-334 2094-down-R：
GATTAAACCTCCTTAAATTGCCCAGA GTACTCATTCC
• BSAI-334 2094-up-F：
TTTGGAGGAATTTAACGGGTCTCAGAGTAAGGATGCTAAAAAC
• BSAI-334 2094-up-R：
TTTTTTTGAGGAGACCCATGTTGTCTAGCTCAAGCA
• BSAI-334 2094-sgRNA-F：
TTGAGCTAGACAACATGGGTCTCCTCAAAAAAAGCACCGACT
• BSAI-334 2094-sgRNA-R1：
ACGATAAAGATCGAGATTTTGGCAATACAATACAACTATGCTGTC
• BSAI-334 2094-sgRNA-R2：
ATGCTGTCTAGATCTCCACTATAGTAGGAAAGAATACACG
• Nickcas-seq-F:
TCTTTTGCGCTTCTTGATTAA
• Nickcas-seq-R:
AAGTACTAAAACGGTAAC
• 2094-seq-F:
GCTCTATAGCCTTTGATGC
• 2094-seq-R:
CGCTAGTTATTTGTTCCCATCTG
CTAAGGATCACATCTTTTTCTAAACTAGGGCCCAACCAATCGTATCTTTGACAA
• BSAI-334 2094-down-R：
GATTAAACCTCCTTAAATTGCCCAGA GTACTCATTCC
• BSAI-334 2094-up-F：
TTTGGAGGAATTTAACGGGTCTCAGAGTAAGGATGCTAAAAAC
• BSAI-334 2094-up-R：
TTTTTTTGAGGAGACCCATGTTGTCTAGCTCAAGCA
• BSAI-334 2094-sgRNA-F：
TTGAGCTAGACAACATGGGTCTCCTCAAAAAAAGCACCGACT
• BSAI-334 2094-sgRNA-R1：
ACGATAAAGATCGAGATTTTGGCAATACAATACAACTATGCTGTC
• BSAI-334 2094-sgRNA-R2：
ATGCTGTCTAGATCTCCACTATAGTAGGAAAGAATACACG
• Nickcas-seq-F:
TCTTTTGCGCTTCTTGATTAA
• Nickcas-seq-R:
AAGTACTAAAACGGTAAC
• 2094-seq-F:
GCTCTATAGCCTTTGATGC
• 2094-seq-R:
CGCTAGTTATTTGTTCCCATCTG