Difference between revisions of "Team:Fujian United/Notebook"

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<div class="column full_size">
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<link rel="stylesheet" type="text/css"
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      href="https://2021.igem.org/wiki/index.php?title=Template:Fujian_United/StyleCSS&action=raw&ctype=text/css"/>
  
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<h1>Notebook</h1>
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<div class="alpha-luco-content">
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<p> Document the dates you worked on your project. This should be a detailed account of the work done each day for your project.</p>
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    <!--navbar-->
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    <div class="navbar">
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        <a class="logo" href="https://2021.igem.org/Team:Fujian_United">
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            <img src="https://static.igem.org/mediawiki/2021/f/f3/T--Fujian_United--logo.jpeg" alt="">
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        </a>
  
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</div>
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        <!--nav-->
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<div class="clear"></div>
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        <ul class="list">
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            <!--Team-->
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            <li class="item">
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                <a href="https://2021.igem.org/Team:Fujian_United/Team">Team</a>
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                <div class="child-nav">
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                    <ul class="child-list">
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                        <li class="child-item">
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                            <a href="https://2021.igem.org/Team:Fujian_United/Team">Team Members</a>
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                        </li>
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                        <li class="child-item">
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                            <a href="https://2021.igem.org/Team:Fujian_United/Attributions">Attributions</a>
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                        </li>
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                        <li class="child-item">
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                            <a href="https://2021.igem.org/Team:Fujian_United/Collaborations">Collaboration</a>
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                        </li>
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                        <li class="child-item">
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                            <a href="https://2021.igem.org/Team:Fujian_United/Partnership">Partnership</a>
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                        </li>
 +
                    </ul>
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                </div>
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            </li>
  
 +
            <!--Modeling-->
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            <li class="item">
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                <a href="https://2021.igem.org/Team:Fujian_United/Model">Modeling</a>
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            </li>
  
 +
            <!--Entrepreneurship-->
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            <li class="item">
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                <a href="https://2021.igem.org/Team:Fujian_United/Entrepreneurship">Entrepreneurship</a>
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                <div class="child-nav">
 +
                    <ul class="child-list">
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                        <li class="child-item">
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                            <a href="https://2021.igem.org/Team:Fujian_United/Entrepreneurship">Entrepreneurship</a>
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                        </li>
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                        <li class="child-item">
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                            <a href="https://2021.igem.org/Team:Fujian_United/Business_Plan">Business Plan</a>
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                        </li>
 +
                    </ul>
 +
                </div>
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            </li>
  
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<div class="column two_thirds_size">
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            <!--Implementation-->
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<h3>What should this page have?</h3>
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            <li class="item">
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<ul>
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                <a href="https://2021.igem.org/Team:Fujian_United/Implementation">Implementation</a>
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<li>Chronological notes of what your team is doing.</li>
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            </li>
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<li> Brief descriptions of daily important events.</li>
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<li>Pictures of your progress. </li>
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<li>Mention who participated in what task.</li>
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</ul>
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</div>
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            <!--Human Practice-->
 +
            <li class="item">
 +
                <a href="https://2021.igem.org/Team:Fujian_United/Human_Practices">Human Practice</a>
 +
                <div class="child-nav">
 +
                    <ul class="child-list">
 +
                        <li class="child-item">
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                            <a href="https://2021.igem.org/Team:Fujian_United/Human_Practices">Integrated Human
 +
                                Practice</a>
 +
                        </li>
 +
                        <li class="child-item">
 +
                            <a href="https://2021.igem.org/Team:Fujian_United/Communication">Communication</a>
 +
                        </li>
 +
                        <li class="child-item">
 +
                            <a href="https://2021.igem.org/Team:Fujian_United/Fundraising">Fundraising</a>
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                        </li>
 +
                    </ul>
 +
                </div>
 +
            </li>
  
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<div class="column third_size">
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            <!--Parts-->
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<div class="highlight decoration_A_full">
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            <li class="item">
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<h3>Inspiration</h3>
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                <a href="https://2021.igem.org/Team:Fujian_United/PartsCollection">Parts</a>
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<p>You can see what others teams have done to organize their notes:</p>
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                <div class="child-nav">
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                    <ul class="child-list">
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                        <li class="child-item">
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                            <a href="https://2021.igem.org/Team:Fujian_United/Parts_Collection">Parts
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                                Collection</a>
 +
                        </li>
 +
                        <li class="child-item">
 +
                            <a href="https://2021.igem.org/Team:Fujian_United/Engineering">Engineering</a>
 +
                        </li>
 +
                    </ul>
 +
                </div>
 +
            </li>
  
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<ul>  
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            <!--Project-->
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<li><a href="https://2018.igem.org/Team:Munich/Notebook">2018 Munich</a></li>
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            <li class="item active">
 +
                <a href="https://2021.igem.org/Team:Fujian_United/Description">Project</a>
 +
                <div class="child-nav">
 +
                    <ul class="child-list">
 +
                        <li class="child-item">
 +
                            <a href="https://2021.igem.org/Team:Fujian_United/Description">Description</a>
 +
                        </li>
 +
                        <li class="child-item">
 +
                            <a href="https://2021.igem.org/Team:Fujian_United/Experiments">Experiments</a>
 +
                        </li>
 +
                        <li class="child-item">
 +
                            <a href="https://2021.igem.org/Team:Fujian_United/Results">Result</a>
 +
                        </li>
 +
                        <li class="child-item">
 +
                            <a href="https://2021.igem.org/Team:Fujian_United/ProofOfConcept">Proof of Concept</a>
 +
                        </li>
 +
                        <li class="child-item active">
 +
                            <a href="https://2021.igem.org/Team:Fujian_United/Notebook">Notebook</a>
 +
                        </li>
 +
                        <li class="child-item">
 +
                            <a href="https://2021.igem.org/Team:Fujian_United/Safety">Safety</a>
 +
                        </li>
 +
                    </ul>
 +
                </div>
 +
            </li>
  
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<li><a href="https://2019.igem.org/Team:Georgia_State/Notebook">2019 Georgia State</a></li>
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            <!--Home-->
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<li><a href="https://2019.igem.org/Team:Newcastle/Notebook">2019 Newcastle</a></li>
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            <li class="item">
 +
                <a href="https://2021.igem.org/Team:Fujian_United">Home</a>
 +
            </li>
 +
        </ul>
 +
    </div>
  
 +
    <!--图片-->
 +
    <div class="page-header">
 +
        <img src="https://static.igem.org/mediawiki/2021/9/94/T--Fujian_United--bg_1.png" alt="">
 +
    </div>
  
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<li><a href="https://2020.igem.org/Team:IISER-Pune-India/Notebook">2020 IISER Pune India</a></li>
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    <!--内容-->
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<li><a href="https://2020.igem.org/Team:Lund/Notebook">2020 Lund</a></li>
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    <div class="content-middle-outer">
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<li><a href="https://2020.igem.org/Team:NOVA_LxPortugal/Notebook">2020 NOVA LxPortugal</a></li>
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        <div class="content-middle">
−
<li><a href="https://2020.igem.org/Team:RDFZ-China/NoteBook">2020 RDFZ China</a></li>
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            <section class="article p-t-30 p-b-54">
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</ul>
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                <h1 class="content-header">Notebook</h1>
 +
 
 +
                <table>
 +
                    <tr>
 +
                        <td colspan="2"
 +
                            style="background: #F7CAAC; font-size: 18px !important; font-weight: bold; line-height: 52px">
 +
                            July
 +
                        </td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td style="width: 20%">4<sup>th</sup></td>
 +
                        <td>Prepare culture mediums.</td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td>5<sup>th</sup></td>
 +
                        <td>Amplify the plasmid fragments by PCR, gel electrophoresis and recycle DNA.</td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td>6<sup>th</sup></td>
 +
                        <td>Construction of the 4 plasmids.</td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td>7<sup>th</sup></td>
 +
                        <td>Colony PCR, gel electrophoresis, and select single clones.</td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td>8<sup>th</sup></td>
 +
                        <td>Plasmids extraction from E. Coli, and sent to the DNA sequencing company.</td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td>9<sup>th</sup></td>
 +
                        <td>Verify the DNA sequencing resuls and carry out the transformation of plasmids into the
 +
                            Saccharomyces cerevisiae by Heat-Shock approach.
 +
                        </td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td>11<sup>st</sup></td>
 +
                        <td>Prepare competent cells of the Saccharomyces cerevisiae, and incubate the yeast single colony in
 +
                            1ml culture mediums.
 +
                        </td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td>12<sup>nd</sup></td>
 +
                        <td>Day off. Due to the limited time of incubation, our Saccharomyces cerevisiae didn't demonstrate
 +
                            efficient growth. Hence, we were not able to carry out the enzyme acitivety tests.
 +
                        </td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td>13<sup>th</sup></td>
 +
                        <td>Carry out fermentation performance test by testing the concentrations of glucose and ethanol.
 +
                        </td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td colspan="2"
 +
                            style="background: #F7CAAC; font-size: 18px !important; font-weight: bold; line-height: 52px">
 +
                            August
 +
                        </td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td colspan="2">Repeat the plasmids construction experiments and the yeast culture by AQ yeast.</td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td colspan="2"
 +
                            style="background: #F7CAAC; font-size: 18px !important; font-weight: bold; line-height: 52px">
 +
                            September
 +
                        </td>
 +
                    </tr>
 +
                    <tr>
 +
                        <td colspan="2">Carry out enzyme activity tests and fermentation performance tests.</td>
 +
                    </tr>
 +
                </table>
 +
            </section>
 +
        </div>
 +
    </div>
 +
 
 +
    <!--Footer-->
 +
    <div class="footer">
 +
        <div class="content-middle">
 +
            <hr>
 +
            <span>WeChat Official Account:</span>
 +
            <span class="red">iGEM Alpha Luco</span>
 +
        </div>
 +
    </div>
 
</div>
 
</div>
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</div>
 
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</html>
 
</html>

Revision as of 07:08, 25 September 2021

Notebook

July
4th Prepare culture mediums.
5th Amplify the plasmid fragments by PCR, gel electrophoresis and recycle DNA.
6th Construction of the 4 plasmids.
7th Colony PCR, gel electrophoresis, and select single clones.
8th Plasmids extraction from E. Coli, and sent to the DNA sequencing company.
9th Verify the DNA sequencing resuls and carry out the transformation of plasmids into the Saccharomyces cerevisiae by Heat-Shock approach.
11st Prepare competent cells of the Saccharomyces cerevisiae, and incubate the yeast single colony in 1ml culture mediums.
12nd Day off. Due to the limited time of incubation, our Saccharomyces cerevisiae didn't demonstrate efficient growth. Hence, we were not able to carry out the enzyme acitivety tests.
13th Carry out fermentation performance test by testing the concentrations of glucose and ethanol.
August
Repeat the plasmids construction experiments and the yeast culture by AQ yeast.
September
Carry out enzyme activity tests and fermentation performance tests.
WeChat Official Account: iGEM Alpha Luco