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| | <div class="content"> | | <div class="content"> |
| | <!--ABSTRACT--> | | <!--ABSTRACT--> |
| − | <section class="article p-t-96 p-b-30"> | + | <section class="article p-t-72 p-b-30"> |
| − | <h1 class="title" style="margin: 0 0 64px !important;">ABSTRACT</h1> | + | <h1 class="title">ABSTRACT</h1> |
| − | <p style="font-size: 20px !important;">A new oral vaccine against rotavirus can help children get antibodies | + | <p style="font-size: 20px !important;" class="m-t-24">In the fermentation method widely applied in ethanol production, |
| − | against rotavirus in a needle-free way. It can enter the child's body in the form of oral liquid, capsule,
| + | glucoamylase is an essential raw material, which accounted for a lot of costs. Our project aimed to |
| − | drink, etc. The oral form can facilitate rotavirus patients' treatment and prevent children from developing | + | develop the Saccharomyces cerevisiae in order to self-secrete glucoamylase, thus, reducing the cost also |
| − | resistance. In this project, the construction and characterization of the plasmids for oral rotavirus | + | improving the fermentation efficiency. In the lab, we started off by constructing 4 plasmids, pYES2-ctl, |
| − | vaccine production were completed in order to prepare the target vaccine. Genes for the β subunit of the
| + | pYES2-TGC, pYES2-HGC, and pYES2-IGC, with different promoters, respectively by Golden Gate Cloning and |
| − | heat-labile enterotoxin (LTB) from E. coli and the immunodominant outer core protein VP7 were cloned and | + | then transformed them into the yeast. After selection by several enzyme activity tests and fermentation |
| − | expressed as a fusion protein. After being introduced into Bacillus subtilis, the plasmid could recognize | + | performance tests, we finally obtained the expected engineered yeast and hope our work will contribute |
| − | rotavirus and express antigens LTB-VP7 successfully. This work here set the stage for the future development | + | the alcohol manufacturing in the future.</p> |
| − | of the oral rotavirus vaccine.</p>
| + | |
| | </section> | | </section> |
| | | | |
| | <!--PROMOTIONAL VIDEO--> | | <!--PROMOTIONAL VIDEO--> |
| − | <section class="promotional article p-b-54 p-t-96"> | + | <section class="promotional article p-b-72 p-t-54"> |
| | <h1 class="title">PROMOTIONAL VIDEO</h1> | | <h1 class="title">PROMOTIONAL VIDEO</h1> |
| | <video src="https://static.igem.org/mediawiki/2021/d/d3/T--Fujian_United--video_index_1.mp4" | | <video src="https://static.igem.org/mediawiki/2021/d/d3/T--Fujian_United--video_index_1.mp4" |
