Team:Ulink-SIP/project/results
Result 1
Obtain CB gene by Polymerase Chain Reaction. Construct the recombinant plasmid pRSFDuet-1-CB-GW by in-fusion cloning. Then transform it into E. coli trans5 alpha. Extract plasmids from transformants. The recombinant plasmids were confirmed by colony PCR and sequencing. Maeker DL 15000 and Marker 2000 are used.
Fig.1 colony PCR of the recombinant plasmid pRSFDuet-1-CB-GW
(Lane 1: Merker DL15000; Lane 2: DL2000; Lane 3~10: GW gene(2090bp) )
Result 2
Obtain rcsA and rcsB by Polymerase Chain Reaction. Then construct rcsAB by overlap PCR. The results were confirmed by agarose gel electrophoresis. Marker 2000 is used.
Fig.2 PCR of the rcsA and rcsB gene (Lane 1:
Merker DL2000; Lane 2~3: rcsA(622bp); Lane 4~5: rcsB(651bp) )
Fig.3 Overlap PCR of rcsAB (Lane 1: Merker DL2000; Lane 2~7: rcsAB(1273bp) )