Team:UNSW Australia/Notebook

iGEM UNSW

This notebook documents the Wet Lab experiments conducted by the 2021 UNSW iGEM team, prior to the Sydney lockdown in late June.

31 May - 4 June


2 June
  • Prepared LB broth and agar
  • Streak plated glycerol stock of E. coli containing HSP22E onto agar to obtain single colonies
3 June
  • Prepared overnight cultures from single colonies of E. coli containing HSP22E
4 June
  • Performed miniprep on E. coli containing HSP22E to extract and purify plasmid for DNA sequencing
  • Conducted restriction digest followed by agarose gel electrophoresis on purified plasmid
  • Streak plated glycerol stock of E. coli containing mCerulean3 onto agar to obtain single colonies

7 June - 11 June


8 June
  • Prepared overnight cultures of E. coli containing HSP22E and mCerulean3
9 June
  • Performed HSP growth assay on E. coli containing HSP22E and mCerulean3

14 June - 18 June


15 June
  • Prepared overnight cultures of E. coli containing HSP22E and mCerulean3
  • Prepared ampicillin stock solution
16 June
  • Performed HSP survival assay on E. coli containing HSP22E and mCerulean3
  • Prepared LB agar
17 June
  • Performed BugBuster protein extraction and SDS-PAGE to check protein expression
18 June
  • Carried out colony counting for survival assay
  • Prepared LB broth and agar

21 June - 25 June


22 June
  • Prepared overnight cultures of E. coli containing HSP22E and mCerulean3
23 June
  • Measured E. coli growth to optimise our experimental design for HSP growth assay
24 June
  • Performed BugBuster protein extraction and SDS-PAGE to check protein expression
This marked our last day in the lab due to the COVID outbreak in Sydney.