These are the parts that we used!
Our project proved that endogenous iron response binding proteins will reduce the translation of transient expression vectors in CHO cells. Iron responsive elements can be inserted into any 5’UTR using our software, Foldase, and researchers will be able to see a steady but decreased translation rate of their desired product. Listed below are the parts we used in our experiments and the Iron Responsive Elements we found to work best. For questions about part usage or design contact Madison Hypes at hypesmadison@gmail.com.
Parts
| Name | Type | Description | Designer | length |
|---|---|---|---|---|
| BBa_K4094000 | Reporter | VSV-G | Madison Hypes | 1536 |
| BBa_K4094001 | Regulatory | Wild-Type IRE-35 Ferritin. 5'UTR containing a translation regulation sequence Iron Responsive Element. Ferritin Wild-Type IRE at position 35 in mammalian expression 5'UTR. | Madison Hypes | 93 |
| BBa_K4094002 | Regulatory | No IRE. Control for stem loop translation regulation. No IRE inserted into the 5'UTR. | Madison Hypes | 57 |
| BBa_K4094003 | Regulatory | WIld-Type IRE-17. Regulatory Ferritin Wildtype IRE in the 5'UTR at position 17. | Madison Hypes | 93 |
| BBa_K4094004 | Regulatory | P2A. Self-cleaving peptide. | Madison Hypes | 66 |
| BBa_K4094005 | Reporter | ddYFP. EYFP with ecDHFR (dihydofolate reductase region). Stabilized by TMP (trimethoprim) ligand. | Madison Hypes | 1197 |
| BBa_K4094006 | Signaling | H2B. Nuclear localization sequence. Histone A. | Madison Hypes | 384 |
| BBa_K4094007 | Terminator | 3' UTR Betaglobin sequence. | Madison Hypes | 228 |
| BBa_K4094008 | Composite | Signaling proteins ddYFP and VSVG separated by P2A. H2B nuclear localization signals attached to ddYFP for cell imaging. | Madison Hypes | 3411 |
| BBa_K4094010 | Regulatory | IRE Control 17. Control stem loop with no binding affinity. At position 17 in 5'UTR. | Madison Hypes | 93 |
| BBa_K4094011 | Regulatory | IRE Control 35. Control stem loop with no binding affinity. At position 35 in 5'UTR. | Madison Hypes | 93 |
| BBa_K4094012 | Regulatory | CMV (cytomegalovirus) promotor enhancer. | Madison Hypes | 380 |
| BBa_K4094018 | Regulatory | SV40 Promotor | Madison Hypes | 380 |