Team Work
Alexandros Skouris:
- Founded, recruited and led iGEM Thrace.
- Found the PIs, Instructor and Advisor of iGEM Thrace.
- Point of contact with PIs, iGEM alumni and many scientists.
- Facilitated brainstorming and decision making for idea & project selection based on current scientific literature, market need and feedback from experts and stakeholders (Integrated Human Practices)
- Urged the team to apply for the pMedGR proteomics grant and provided feedback.
- Took the initiative to involve us in entrepreneurship competitions and coordinated our participation.
- Coordinated the Erasmus + Youth Exchange for Science Communication as well as the European Youth Event participation, both from grant writing to implementation.
- Along with Despina, had organized and moderated the SyntheSci: International Biomedical Students' Conference.
- Created our social media accounts and appealed to many followers.
- Created press releases and gave interviews on radio and TV shows.
- Worked closely with most of the teammates, provided feedback and coordinated general meetings.
Despina Kiousi:
- Participated along with Alexandros and Elisavet in the recruitment of team members.
- Coordinated brainstorming, literature search and experimental design of the Wet lab.
- Provided critical feedback on experimental design.
- Prepared and submitted several Check-In forms and the Safety Form.
- Wrote the proposal and secured the pMedGR proteomics grant. Point of communication between the team and the infrastructure. Prepared a clinical protocol for the collection of samples needed.
- Coordinated the part contribution subteam.
- Participated in the ACEin competition and provided critical feedback on our presentation.
- Provided critical feedback and participated in proofreading of the Erasmus+ Youth Exchange proposal.
- Co-organized and co-moderated the SyntheSci: International Biomedical Students' Conference with Alexandros.
- Participated in the Promotional video.
Vasilis Theologidis:
- Member of the Wet Lab team.
- Brainstorming for the initial idea and project selection.
- Research of scientific literature for the biomarker and method selection.
- Providing guidance and feedback regarding the experimental procedure.
- Mathematical modeling of wet lab reactions in collaboration with Andreas Maniatopoulos and Sotiris Dimitriadis.
- Participation in decision-making concerning the Biological parts.
Elisavet Kapetanou:
- Wet lab team member.
- Brainstorming for project selection, literature search for assay selection and experimental biomarker detection method.
- Performed the wet lab experiments along with Sotiris.
- Co-writing of the Erasmus+ Youth Exchange for Science Communication grant application and preparation of the programme.
- Participated in discussions with scientists, medical doctors, experts of many fields and stakeholders related to our project for feedback integration as a member of the Human Practices team.
- Provided feedback on general calls and project presentations in entrepreneurship competitions.
- Wiki text writing and editing.
- Communication with our team’s instructor, scientists and iGEM alumni.
Ioannis Pariotis:
- Member of the Human Practices team.
- Member of the Fundraising team.
- Biological parts contribution.
- Promotional and Presentation video (Script and Participation).
Ellie Malkopoulou:
- Co-organization of the Erasmus+ programme.
- Member of the Human Practices team.
- Member of the Fundraising team.
- Biological parts contribution.
- Writing pages of Wiki.
- Collaborations with other teams.
- Promotional and Presentation video (Script and Participation).
- Co-organization of the activity for the European Youth Event.
Sotiris Dimitriadis:
- Attracted funding from Bodossaki foundation and Nueria properties, our main contributors.
- Bibliographical research and brainstorming in order to find a single method that can be used for the quantification of the aforementioned biomarkers. Detail search about the Cas12a trans cleavage activity and recombinase polymerase amplification..
- Using Benchling designed oligos for: a)Reverse transcription of mir 766-8p and its amplification with RPA, b)Αmplification of nusG gene of Fusobacterium nucleatum subsp. nucleatum ATCC 25586 c)Guide RNA for cas12a that recognises the amplified products of both aforementioned targets.
- Organised the orders from IDT, Twist, TwistDx and Anadrasis ensuring complete inventory for all wet lab experiments.
- With Elisavet Kapetanou together we run all the experiments of the wet lab.
- Helped dry lab with the design of our 3d printed smartphone fluorimeter.
- Transcribed colorimetry code from matlab to python.
- Organised and run the TEDx DUth synthetic biology workshop.
- Aided in the organization of the iGEM Greek meetup, more specifically in the medical illustration workshop.
Ilias Karabasis:
- Local website & wiki development and guidance.
- General feedback for different aspects of team tasks/issues (presentations, text descriptions, decision making, etc.), sometimes especially with a more general-public point of view due to lack of bio-related expertise.
- Contact with team mentor Konstantinos Ragios to schedule mentoring calls according to team needs.
- Contribution to the organization of the Synthe-Sci conference.
- Contribution to Presentation Video calls and logistics.
- Participation in European Youth Event conference.
- Reached out and found the participants for National Cancer Survivors Day Video.
Nikos Boukistianos:
- Local website development.
- Wiki development.
- Erasmus website development.
- General feedback in team calls and conversations.
Andreas Maniatopoulos:
- Moderator of Dry Lab.
- 3D printable model of color standardization and fluorimeter cube with Sotiris Dimitriadis.
- 3D printable model of microfluidic channel in COMSOL using fluid dynamics.
- Mathematical modeling of wet lab reactions with the help of Sotiris Dimitriadis and Vassilis Theologidis, Enzyme Kinetics and Fluorescence estimation.
- Representative of the team at the Mindspace University Competition along with A. Skouris and T. Tzoumanika.
- Written the color normalization software with the help of S. Dimitriadis.
Emmanouela Gkiozi:
- Co-Design of the team’s local website.
- Co- Design of Wiki page.
- Sponsors Flyer Design.
- Team members illustration.
- Speakers presentation of Synthe-Sci conference.
- Social media posts design (Series of facts, event posters, world days, members' photos in action, etc.).
- JA posts design.
- Collaboration with iGem Düsseldorf and Postcards design.
- General feedback in team calls.
- Writing some parts of pages for wiki.
Triantafyllia Georgouli:
- Sponsors Flyer Design.
- iGEM Thrace Team Logo.
- JA Video Promotion-SaliCa.
- 2x3D model kit designs-Renders(For JA+ΕΡΤ)-Pictures.
- National Cancer Survivors Day Video.
- Several “International day for…”, Calls for members, events, origin cities of the team Posts on instagram.
- Co-Design of the team’s local website.
- SaliCa Project logo design.
- iGEM Thrace Social Media Video.
- Erasmus+ Logo/Event Poster/ Day 3 schedule.
- General feedback in team calls.
- Co-Design Wiki.
Theodora Tzoumanika:
- FundRaising & Entrepreneurship Manager.
- Representative of the team at the Mindspace University Competition.
- Representative of the team at the ACEIN Competition.
- Representative of the team at the JA Competition.
- Co-organization of the Erasmus+ programme.
- Co-organization of the activity for the European Youth Event.
- Contact with Gregory Yovanof to schedule calls.
- Member of social media group.
- Collaborations with other teams.
- Presentation video (Script and Participation).
- Came up with our project name (Salica).
- Helped with WIKI page.
External Work
Aided us in understanding the metabolic pathways that can produce main colorectal cancer biomarkers and pointed towards the importance of early diagnosis.
Helped us understand colorectal cancer physiology and the determination of the advantages and disadvantages of all biological sample types with the according biomarkers. Set the main goals of our biosensor and guided us through our bibliographical research.
Provided us with the laboratory and space to conduct the experiments, as well as guidance and support during the experiments and troubleshooting as well as considerations regarding assay design.
Provided us with valuable insights throughout the course of our project thanks to his extensive experience in the iGEM Competition. Initially we gave us general hints about the several aspects of the competitions, "dos & donts", where to focus our attention more etc. As our project evolved, we also exchanged views around specific aspects of it. Finally, we discussed with him the strongest points of our team to choose which gold medals and special awards to aim for.
Gave us valuable insight into the crucial characteristics to consider into our device and assay design early on while supporting our brainstorming process
Gave us valuable insight into the crucial characteristics to consider into our device and assay design early on while supporting our brainstorming process
Informed us about innovation in biotech and current trends in biomedical sciences.
Consulted us about patent search and patent law with the goal of examining if our project can be patented in any way. We were also motivated to analyze the novel aspects of our biosensor in order to take them a step further so that
Guided us for the selection of the electrochemical sensor for the planned potentiostat model
Providen us with information and literature about thin-film transistors, materials for detecting biomarkers in saliva and detection challenges.
Entrepreneurship mentoring: Mentoring on Market need & business plan. Provided us with specific material in order to help us write out the team's business plan.
Addressed that we should take the cost of the device into account and try to make it as affordable as possible, expand our devices capabilities to screen for post operational colorectal cancer biomarkers in order to ease the patient and the doctor when doing a follow up from surgery.
George Apidianakis
Stated that we must find arguments beyond the ease of sampling, and delve deeper the according publications about salivary colorectal cancer biomarkers.
Konstantina Tsoumani
Informed us about the latest research about CRISPR Cas diagnostics and consulted us about the technical details of the SHERLOCKv2 protocol. Also she helped us focus on the needs of the final customer that will use our biosensor in order to make it easier to use and sacrifice low cost to get higher sensitivity because it is more important for a diagnostic device.
Provided us with valuable insight regarding assay and product development in relation to market needs
Gave us feedback about the value of early diagnosis vs relapse monitoring and stated the importance of choosing the right biomarkers. He also made clear that our next step, after our lab experiments, is a clinical trial where the device will be tested in patients at an early cancer stage vs healthy ones who have had a colonoscopy and do not have cancer. And also informed us about the steps for creating an according protocol for the clinical trial and gave us guidelines on how to get permission for the trial from the university bioethics committee and the scientific council of the hospital.
Petros Kolovos
Provided valuable feedback regarding the selection of the kind of the biomarkers we targeted.
Provided valuable feedback regarding the selection of the kind of the biomarkers we targeted.
Informed us about the current state of the diagnosis of gastrointestinal disorders and shared his opinions with us about the medical and patients’ issues and needs.
Gave us feedback on our project and many tips about iGEM competition throughout our journey.
Gave us feedback on our project and many tips about iGEM competition throughout our journey.
Participated actively in general meetings and provided feedback for a long period of time.