Team:TJUSLS China/Judging
Medal Requirements
This web page is made to ease judges the review of medal criteria. We hope to have a good harvest of GOID MEDAL after summer.
GOLD
Integrated Human Practices
Human Practices work has deeply influenced the conception, design and stage of our project. We studied Plastic Package market up and down our project purpose. Our consultation with experts helps us design our projects. Feedback and experience from companies and governments help us design projects for use. Please visit Integrated Human Practices pageto see more.
Improvement of an Existing Part
This year, we tried to improve the degradation efficiency of PETase by improving the thermal stability of PETase. We’ve registered many improved parts of PETase this year. Among them, as an improvement of the existing part BBa_K2010000, BBa_K3715005 shows extraordinary thermostability. For more information, check out our Parts page.
Project Modeling
We built the Mathematical Modeling: UV-HPLC to simplify complex experimental operations. We also evaluated the thermal stability of mutants by Molecular Dynamic Simulations. Homology modeling was conducted using Alfold and affinity for ligands was calculated using Docking. Please visit Modeling pageto see more.
Partnership
We have established in-depth partnership with BJEA_China, DUT_China. We have the same goals and both benefit from the collaboration. Please visit Partnership page to see more.
Education & Communication
Due to the epidemic, we chose bilibi podcast and other platforms to introduce synthetic biology to people of all ages and proverty through online live courses as materials. Please visit Education pageto see more.
SLIVER
Engineering Success
We implemented three iterations of the engineering design cycle. Please visit Engineering pageto see more.
Collaboration
We have established cooperation with BJEA_China, DUT_China, Toronto, Plasticase, Shanghai SJTU-Biox and ShanghaiTech_China through Tencent conferences and offline communication. Please visit Collaboration pageto see more.
Human Practices
The reason we chose this project was because of our concern for the environment, we further carried out some background retrieval. To ensure that our project is beneficial to the society, we conducted some social interviews, including enterprise research and government interviews. Please visit Human Practices pageto see more.
Proposed Implementation
Our target users are companies that produce, recycle and reuse PET products, waste treatment plants, environmental organizations and so on. We designed an industrial system that included the design of the fermentation tank and the processing process. Please visit Implementation pageto see more.
BRONZE
Competition Deliverables
Our Wiki, Presentation Video and Judging Form have been uploaded as scheduled. Please visit our Home pageand Judging Form pagefor more details.
Attributions
Our members have all played an important role in our projects, and many companies and experts have provided additional support. We learned a lot from the project and are very grateful to those who provided support and assistance in our project. Please visit Attributions pageto see more.
Project Description
Our aim is to improve the degradation rate of a novel PET degradation enzyme named PETase. We tried to improve the thermal stability of PETase and finally improve the degradation efficiency of PETase. Please visit Description pageto see more.
Contribution
Our contributions for future iGEM teams are as follows:
- Parts:Seven PETase mutants’ thermal stability were greatly improved
- Found rules to stabilize protein
- Constructed a testing method of a catabolic enzyme.
- Established a method which can accurately predict protein structure
- Developed a conversion model between UV260 and HPLC Peak Areas
