Team:TJUSLS China

What is PET?
Poly ethylene terephthalate, commonly abbreviated as PET, is produced by the chain polymerization of ethylene glycol and terephthalate. Due to its durability, low price, and convenient processability, PET becames one of the most widely used synthetic plastics worldwide, with an annual manufacturing capacity of
over 30 million tons.
PET has various applications in our daily life and industries, such as in manufacturing bottles, pressure-sensitive tapes, textiles, containers and so on.
crystallinities of PET
According to its applications, PET has different crystallinities. For example, PET used for packaging has less crystallinity, e.g. approximately 8%.
PET used for manufacturing bottles and textiles has
high crystallinity of 30-45%, which can't be degraded by LCC and remains a huge problem.
Highly crystallized PET
Our team aim to degrade highly crystallized PET because the stability of it slows down the degradation and cause great damage to the nature.
How to deal with
highly crystallized PET?
PETase makes the degradation of highly crystallized PET possible.Yoshida et al. isolated a novel bacterium, Ideonella sakaiensis 201-F6, which is able to use PET as its major energy and carbon source.
Wild type PETase loose activity
within 24 hours. Considered that we use fermenter as reaction site, we have to add enzyme frequently to keep degradation going on.
The lack of thermostability
are inevitable problems, thus our goal is to improve it, so that it could degrade PET more efficiently.
Our Solution
——To improve Thermostability of PETase
Most of the existing work focuses on the transformation of its
degradation activity
and improve it with little attention paid to the thermostability of PETase.
we found that thermostability largely restricted PETase to play its due role.
Our methods
Through the study of the homologous enzymes of PETase, we discovered the possibility of improving thermostability of the protein through site mutations and thereby enhancing the activity of the protein. Different mutations stabilize different sites of PETase through specific protein internal foece .
Now we have
Learn more about our project...
Based on the above methods, we rationally design
a series of single point mutations, and combine
them to generate multiple point mutations.
Learn more abour our DESIGN.
Combinatorial mutation, MD and Docking are
employed to verify the results.
Learn more about our DTY LAB.
Investigation and interviews are used to
help us to find out the significance. Online lectures and
podcasts are applied to spread the charm of
synthetic biology.
Learn more about our HUMAN PRACTICES.
Address: No.92 Weijin Road,Nankai District,