Proposed Implementation
We propose to implement this project into the real world by designing a hardware. Since we have engineered some E.coli bacteria to detect the CSP signal, we must find a way for customers to test their mouth environments without spreading the E.coli bacteria. After researching and modifying, we settle down on a hardware that looks like the picture below to eliminate safety concerns regarding the use of the engineered E.coli cells.
Figure 1: Our product's hardware.
Since our intended audience will anyone who cares about their dental health, and especially parents who care a lot for their babies, the audience is broad and is spread around the world. People living in developed countries and developing countries typically care more about dental health, and so these people are a particular focus of our . More information about the product can be found on the Hardware page.
We also wrote a simple procedure to allow users to use our hardware.
- Rinse your mouth to collect the sample of your mouth environment
- Spit the rinsed water into the opened hardware and make sure the water level passes the filter (the tube)
- Close the tube and shake gently
- Put the tube in the baby warmer bottle and set it at 37 degrees
- Wait for 12-24 hrs and check the color degradation
- If there is little to no color degradation—congratulations! Your mouth is free of S.mutans. Otherwise, you need to work on brushing your teeth and visiting the dentist.
Some of the current challenges we have met is that the product will not work as our circuit is not completely functional right now. Also, there is a question of wasting the tubes because each tube can only be used once, as there is no RR color if the tubes are to be reused. Also, it is still uncertain whether the CSP signal from the rinse water will be enough to be detected by the E.coli.