Team:THIS-China/Attributions

Abstract

Dental plaque is an ecological environment in which bacteria grow, reproduce, decay, and carry out a series of metabolic activities. In this project, an E. coli biosensor is built to activate the phosphorylation of ComE membrane protein and ComD protein in sensor cells and activate the downstream CotA gene to cause color degradation. This sensor cell uses short-length peptide CSP secreted by Streptococcus mutans as a signal to detect the amount of S. mutans in the patient's mouth. To demonstrate this, contactless cell-free system hardware is designed. The customers can use the oral rinse to collect the sample and place into the device to see color degradation. The product is portable and reasonably priced, thus allowing more customers to have the ability and willingness to purchase it.

Student Members

Richard Hou: Student Leader

Richard recruited the team, chose the topic, settled collaborations with other teams, did work in the wet lab, helped with the HP, did some wiki writing, and checked all of the writing in the wiki.

Calvin Chen: Member

Calvin made the promotion video and an educational video, made a survey for the HP, did work in the wet lab, and did some wiki writing.

Devin Chen: Member

Devin was the head for hardware making. He did work in the wet lab, supported our work in collaboration and HP, and did some wiki writing.

Kelly Chen: Member

Kelly helped choose the topic, did work in the wet lab, designed our team logo, supported our work in collaboration and HP, bought things we need, and did some wiki writing.

Kevin Hou: Member

Kevin helped set the topic, did work in the wet lab, supported our work in collaboration and HP, did entrepreneur research, and did some wiki writing.

Ben Hwang: Member

Ben did work in the dry lab, supported our work in collaboration, and made a preliminary draft for the website.

Audrey Sun: Member

Audrey helped choose the topic, did work in the wet lab, did some wiki writing, and did most of the drawings.

Christopher Shi: Member

Christopher helped set up the topic, did work in the wet lab, did some wiki writing, and found places for our experiments and HP.

Hanlin Wang: Member

Hanlin helped with the hardware design, helped set up the topic, did work in the wet lab, did some wiki writing, and supported our HP and collaboration.

Gavin Wang: Member

Gavin did work in the dry lab, did the mathematical modeling, supported our work in collaboration and HP, did some wiki writing and checked grammar.

Wendy Wang: Member

Wendy helped choose the topic, helped with art and drawing, did work in the wet lab, did some wiki writing, and supported our HP and collaboration.

Andrew Li*: Outsider Helper

Andrew is the leader of THIS's coding club and did the final layout, design, and coding for the entire website. His GitHub profile can be found at this link.

Advisors

Fatima Hachem: Primary Instructor

Ms. Hachem helped us put together the whole project. She was in charge of the payments and registration, and also gave us essential advice on different parts of our project.

Xuan Wang: Secondary Instructor

Xuan Wang taught us basic lab safety and lab techniques, and gave us essential advice for our experiment, HP, and collaboration.

Xiaofei Ge

Xiaofei Ge helped us research and set up our topic, built up the basic part of our iGEM project, and helped us on certain problems we met.

Yafei Li

Yafei Li helped us determine the different experiments needed in the lab and taught us the procedures of specific labs.

Other Teams

The wiki page of Team CCU_Taiwan 2017 and SZPT-CHINA 2020 has helped our team immensely on experiment protocols and basic information.

Tsinghua

The Tsinghua team helped us settle down the project, solved some problems we met during the experiment, and communicated about HP, hardware, and website design. We also led promoting synthetic biology with them.

QHFZ

QHFZ and our team held joint meetings. We borrowed equipment from each other's labs, and checked each other’s projects.