Welcome to Siberia team wiki!
We are the first iGEM team from Siberia and we are going to defy obesity working on our project
This is how our project is called. What is the idea?
We thought long and hard about what problem we should tackle with the help of synthetic biology. What is currently relevant? What can we realistically and successfully do in the limited four-month timeframe, that is still ambitious and challenging? The choice fell on the treatment of obesity with living therapeutics. Our attention was drawn to LEAP-2, a rather interesting protein, which is able to regulate appetite and satiety, thereby influencing human eating behaviour. For a therapeutic effect, this protein must be periodically supplied directly into the brain.
Here is the gist of our project: we are planning to create a strain of bacteria that produces LEAP-2, which can be implanted into a person's nose for constant synthesis of the desired product. With this approach, the protein will be delivered directly to the brain via the olfactory and trigeminal nerves, bypassing the bloodstream and the blood-brain barrier.
The overall goal of our project is to provide a proof of concept for the development of living therapeutics working on the principle of nose-to-brain delivery. We have plans to test our system on mice and hope to present our first results during the course of the competition!
As we've said in our introductory post, the main idea of our project is to create a bacterium that will secrete LEAP2 into the nose, after which the protein will travel to the hypothalamic region of the brain via the trigeminal nerve, and bind to a receptor of the hormone Ghrelin (GHSR1a).
So, why did we choose LEAP-2? In the early stages of project development, it actually caught our attention because of its recently discovered physiological effect. Several studies have shown that an increase in the ratio of LEAP2/Ghrelin concentration in the blood will be beneficial for weight loss, due to an onset of a feeling of satiety and hence a decrease in food intake. To visualize the protein secreted by the bacterium, we decided to use an epitope of the C-myc protein — Myc-tag, and to facilitate its entry into the brain — a cell-penetrating peptide called Low molecular weight protamine. Combined with a Myc-tag and LMWP, the fusion protein will have a length of 65 amino acids, and we hope that the small size will facilitate secretion from the bacteria.
Though we are not planning on demonstrating the physiological effect of the protein, we carried out in silico modelling of the putative structure of the fusion protein to ensure that the Myc-tag and the N-terminus are open, since according to various sources, the first 10-14 amino acids are sufficient for binding to the GHSR1a receptor.
Styling your wiki
You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.
While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.
Uploading pictures and files
You must upload any pictures and files to the iGEM 2021 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name.
When you upload, set the "Destination Filename" to T--YourOfficialTeamName--NameOfFile.jpg. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)
Wiki template information
We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the Pages for awards link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!
Editing your wiki
On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world!
Use WikiTools - Edit in the black menu bar to edit this page
This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started:
- State your accomplishments! Tell people what you have achieved from the start.
- Be clear about what you are doing and how you plan to do this.
- You have a global audience! Consider the different backgrounds that your users come from.
- Make sure information is easy to find; nothing should be more than 3 clicks away.
- Avoid using very small fonts and low contrast colors; information should be easy to read.
- Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the iGEM 2021 calendar
- Have lots of fun!