Team:SHSBNU China/Collaborations
SHSBNU_China & GreatBay_United
The team logo of GreatBay_United
GreatBay_United’s goal of the project is to use Bacillus subtilis to produce artificial Horseshoe crab blood which is a scarce resource that can be used in detecting bacterial toxins. With this project, they hope they can help to reduce horseshoe crab trade and save this ancient species. The B. subtilis that we used in our project is the strain that they shared us. However, due to the lack of experience, the transformation of the B. subtilis was not successful. GreatBay_United did success in the transformation. In the hope of learning from the experience of the GreatBay_United, we had some in-depth discussion of their success experience. In those discussions, our team have realized some of the errors that we made, namely, the wrong shaking frequency of the shaker that we set for 200rpm instead of 90 rpm, resulting in the death of the competent cells. Although we have formed plans of solving this problem, our B. subtilis transformation experiment cannot be continued due to the limited time and the lacking of competent cells.
CCiC Beijing District Meeting
On April 18th, 2021, we presented our preliminary design of our project about recycling radioactive Iodine from nuclear wastewater with the iodine pump found in seaweeds. In this meetup, students and
instructors from numerous teams have given precious advice and well-constructed questions to us regarding the presented project. With those advice and questions, we realized that the project itself must suffice the following criterions: safe for students to experiment, can be finished in limited time, and appliable to other fields.
After the meeting, we then improved our project design to meet those criterions. By changing our project design to developing a precious metal recycling platform, we avoided the use of radioactive materials that are extremely dangerous for high school students to operate, and we are able to finish the experiments in a limited time. Also, because this platform is based on aptamer-ion interactions, it can be applied to various fields not limited to any single ion.
Meetup
A team member from NDNF_China is presenting their project in the meetup.
A team member from RDFZ_China is presenting their project in the meetup.
Mr. Wang, the instructor of BJU_China, is presenting their project in the meetup.
The group photo of BIT(left) and us(right).
On July 28th, 2021, SHSBNU_China hosted a meetup with BIT& BJU_China& NDNF_China& RDFZ_China at Bluepha Laboratory. In this event, each team introduced their project and presented some problems or new-found solutions of those problems. At that time, we were obstructed by the problem of how to purify our wanted proteins. When we presented this problem to the other teams during the meetup and much more solutions have been suggested. Ranging from using higher sonication voltage and longer time to changing the present protein to a larger one. The suggestion that was the most innovative was given by instructor Elise from NDNF_China that it was the formation of inclusion bodies that caused the failure of protein purification. From this inclusion hypothesis, we made several changes to our experiment: first, we reduced the incubation time after the induction of IPTG in hope of the reduced induction time can decrease the inclusion body formation; second, we reduced the incubation temperature in hope of the lower temperature can slow down the protein formation rate thereby giving peptides with enough time to fold properly.
SHSBNU_China & GCGS_China
GCGS_China aims to use BCNF membrane (Bacterial Cellulose Nano-Fiber membrane) and aptamers to create a test paper that can detect QS factor, a substance released during seafoods spoilage, thereby indicating whether did the seafoods spoil. Since our team had successfully produced large quantity of BCNF, we shared our cellulose membrane to them so that they can use them for the test paper and compare the quality of ours and those they brought.
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