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Gold Collaboration
Gold Partnership with Osiris Rio UFRJ iGEM Team
Figure 7: Meeting with the Osiris Rio UFRJ iGEM Team
The Osiris RIO UFRJ iGEM team worked on detecting dengue disease with an electrochemical biosensor. Our team met with the Osiris RIO UFRJ iGEM team to discuss our projects on July 30th. Osiris RIO UFRJ team has worked with immobilizing proteins on the electrode. Since we were close to attaching aptamers to the screen-printed electrode, they advised us to carefully cast aptamers on the working electrode and not drop on the reference and counter electrode. They also advised us to use a potentiostat to measure cyclic voltammetry before and after the electrode has been modified with rGO and aptamers. Our team received very beneficial advice from the Osiris Rio UFRJ Team on how to use cyclic voltammetry, including what voltage difference and voltage scan rate to use. Because the Osiris Rio UFRJ had experience using a 3-electrode system measuring potential, they also provided us with insights on making 3D printed electrodes and using cyclic voltammetry to characterize the modified electrodes to ensure that modifications worked. They sent us the protocol for cyclic voltammetry that they conducted.1
Since both of our teams worked on a drop casting solution on the working electrode, our team offered help with using the drop-casting method to modify screen-printed electrodes, where we used a needle to drop small volumes of reduced graphene oxide on the top of our working electrode, and then, we used nitrogen flow to dry the solution. We offered to send this protocol to them as they also worked on modifying the surface of the electrode with the immobilized protein. The Osiris RIO UFRJ iGEM team also developed a different technique to overcome the issue of modifying the electrode. Additionally, we offered our expertise with Onshape software, which is important for designing 3D parts. They asked for help on the 3D design of the mask which could help them to properly paint screen-printed electrodes with carbon ink. Our team designed the mask in Onshape according to the dimension they sent us, which would help their team speed up the process of modifying their 3D electrodes.
Figure 6: The eletrode mask we desinged for the Osiris RIO UFRJ iGEM team using Onshape. After designing our software, we wanted to know whether it could be easily used by other iGEM teams and in different diagnostic projects. To test the ease of use of the software, we asked the Osiris RIO UFRJ iGEM team to use their voltage values for dengue biomarker with our software and gained some very valuable input to make the software more user friendly. They provided us with their data, which consists of the voltage and current change of a Dengue biomarker, Denv2 multi-epitope protein, with gold nanoparticle thiol modification in different concentrations. By plugging in their data, they showed that our software can successfully convert potential and current to resistance, and that they are able to obtain the sample plot of plasma biomarker concentration over time using our biomarker equation. Their feedback helped us improve the way we commented on our code thereby improving ease of use.
References
- Elgrishi, N., Rountree, K. J., McCarthy, B. D., Rountree, E. S., Eisenhart, T. T., & Dempsey, J. L. (2018). A Practical Beginner’s Guide to Cyclic Voltammetry. Journal of Chemical Education, 95(2), 197–206. https://doi.org/10.1021/acs.jchemed.7b00361