of oil released into the environment as a result of tanker incidents
Although the total number of Oil Spill incidents is decreasing each year, previous spills have a compounding negative impact on the environment. Oil pollution is very hazardous to marine flora & fauna, especially when left untreated for a long period of time.
Biodiversity of Caspian Sea is under crisis because of oil pollution
Kazakhstan has more than 250 oil fields, majority of which are located in the Caspian Sea. It is recognized as independent zoogeographical region due to its high endemism. Oil pollution places endemic organisms of the Caspian Sea under the threat of extinction.
OIL SPILL RESPONSE
Applying microorganisms is more cost effective and environmentally friendly method of treating oil spills. It is also efficient in long-term management of oil pollution.
Pseudomonas aeruginosa are bacteria carrying both nadE and rhlA/B gene that encode for NAD synthetase and rhamnolipid producing enzymes respectively.
Produced rhamnolipids are biosurfactants that can be used for crude oil bioremediation. However, along with rhamnolipids, P. aeruginosa can produce toxins as well.
Hence, we aim to modify less toxic relative of P. aeruginosa Pseudomonas putida in a way that would allow production of rhamnolipids.