Team:HKUST/Notebook

ShellBi

Project
Description Design Engineering Proof of Concept Implementation Safety Contribution Notebook
Wet Lab
Experiments Results
Dry Lab
Model
Reach
Human Practice Collaboration Communication Education Sustainable
Parts
Basic Parts Composite Parts
Team
Team Attributions

Notebook

Our Journey and Log Book

WELCOME TO OUR JOURNEY

Here is Our Wetlab Logbook!

General Wet Lab Dry Lab Circuit Design Human Practice Graphic Design Wiki

APR

Propose potential iGEM projects Literature review on chassis choice for wet lab

MAY

Model drafting for ion influx and effect of STX/VTD Literature review on bacterial osmoregulation Team logo design Wiki design planning Photo taking of members YouTube channel & Instagram account setup Team introduction Timeline setting

JUN

Circuit with Two Component System regulated fluorescence proteins confirmed Literature review on protein structure Literature review on ion channel Literature review on C. raciborskii, V. fischeri, A. circinalis Promotional video made Educational social media postsmade Interview requests sent to various stakeholders Collaboration proposals sent to various teams Meeting with Professor Qing Lu Zeng First meeting with HK Oyster

JUL

Scaffold and auto-inhibition domains on EnvZ and OmpR designed Making competent cells Antagonism of STX and VTD simulated by LB with different NaCl concentration Tried cloning and collecting data for TCS basal Meeting with Professor Aifeng Li Designed antisense RNA against fluorophores under respective promoters Integrated the scaffold as a feedback loop in the circuit Drafted PompC/PompF, antisense and scaffold model Simulation of TCS model and whole circuit Hong Kong regional iGEM symposium preparation First meeting with Professor Brett Neilan and Mr. Mark Van Asten Meeting with Dr. Terence Chung of the Hong Kong Agriculture, Fisheries, and Conservatory Department

AUG

Calibration of osmometer and collection of data Collected data on antisense RNA fragments Conducted experiments for HK_GTC iGEM team Completion of phylogenetic tree Literature review on sensitivity analysis Antisense model updated Education booklet published Plan timeline for wiki writing and file uploading Hong Kong regional iGEM symposium 2021 Project introduction video for YouTube Meeting with Mr. Mark Van Asten Meeting with Professor Brett Neilan

SEPT

Collecting data on various constructs in LB of different salt concentration Cloning of engineered EnvZ and OmpR into the TCS with scaffold- unsuccessful Characterising the negative regulator region on PompF Chassis choice for implementation finalized Literature review on mathematical model for ion influx Sensitivity analysis Wiki coding Meeting with Professor Stanley Lau Meeting with Professor Brett Neilan and Mr. Mark Van Asten Videos on project background for YouTube Meeting with HK Oyster

OCT

Second trial on cloning the scaffold and engineered EnvZ/OmpR Compile all data from all experiments Structural modeling of toxin docking on ion channel Wet lab data fitting Design change to all wiki pages for coherency and readability Code uploaded to Wiki server Educational Booth set up in HKUST Meeting with Professor Brett Neilan and Mr. Mark Van Asten InnoCarnival

General

Circuit Design

Wetlab

Drylab

Human Practices

Wiki

Graphic Design