Team:HKUST/Composite Parts

ShellBi

Project
Description Design Engineering Proof of Concept Implementation Safety Contribution Notebook
Wet Lab
Experiments Results
Dry Lab
Model
Reach
Human Practice Collaboration Communication Education Sustainable
Parts
Basic Parts Composite Parts
Team
Team Attributions

Composite Parts

Detailed Explanations of Our Parts

BBa_K4061070- TCS basal

Parts - BBa_K4061070

Two Component System basal sequence or TCS basal for short, consists of 2 different promoters attached to 2 different reporter genes. Originally, the two component system is a system found in E.coli that responds to differences in osmolarity conditions inside the cell. PompC responds to a high intracellular osmolarity, while PompF responds to a low intracellular osmolarity. We attach an eGFP reporter gene to the OmpC promoter and mRFP1 reporter gene to the OmpF promoter. This system suits the purpose of our project since our biosensor detects toxins that block the porin protein of the cells which in turn cause a difference in intracellular osmolarities.