Team:HKUST/Basic Parts

Parts Number	Parts Name	Description
BBa_K4061000	OmpF promoter	OmpF promoter will be activated under low intracellular osmolarity conditions. This part contains the negative regulator region, F4 at -360bp.
BBa_K4061002	eGFP	eGFP is a mutant form of GFP that has a higher fluorescence intensity and better photo-stability as compared to the native GFP. This particular eGFP sequence has been codon-optimized for usage in E.coli.
BBa_K4061020	Engineered EnvZ*	The engineered EnvZ contains the native EnvZ with the addition of SH3 domain on its C terminus and an auto-inhibition domain which is an SH3 ligand close to its kinase activity domain. The engineered EnvZ has to be used with the scaffold and the engineered OmpR.
BBa_K4061010	Engineered OmpR*	OmpR is a native regulatory protein in E.coli that facilitates the expression of the OmpF and OmpC genes. The engineered one is equipped with a leucine zipper. It is meant to work together with the engineered EnvZ and scaffold.
BBa_K4061015	Scaffold*	The scaffold contains a leucine zipper adapter and an SH3 ligand. The scaffold functions to increase the interaction of the engineered EnvZ and the engineered OmpR.
BBa_K4061045	mRFP1 antisense RNA	The mRFP1 antisense has been proven to downregulate mRFP1 (BBa_E1010) that is controlled under RBS (BBa_B0034) and OmpF promoter (BBa_K4061000). Refer to our Results page to look at its downregulating ability.
BBa_K4061040	eGFP antisense RNA	We have previously demonstrated the downregulation of eGFP (BBa_K4061002) that is controlled under RBS (BBa_B0034) and OmpC promoter (BBa_R0082) with this eGFP antisense. Refer to our Results page to look at its downregulating ability.
BBa_K4061041	eGFP antisense RNA(characterization)	This fragment is similar to BBa_K4061040 but this is optimised to regulate constitutively expressed eGFP. Only the -10 promoter region is changed. This was used for the characterisation of antisense RNA efficiency.
BBa_K4061046	mRFP1 antisense RNA (characterisation)	This fragment is similar to BBa_K4061045 but this is optimised to regulate constitutively expressed mRFP1. Only the -10 promoter region is changed. This was used for the characterisation of antisense RNA efficiency.
BBa_K4061110	SH3 domain*	This part is registered separately here so other teams could utilise this domain to enhance protein-protein interactions. However, this modification needs to be added along with the Leucine Zipper domain (BBa_K4061115) and the scaffold.
BBa_K4061115	Leucine Zipper domain*	This part is registered separately here so other teams could utilise this domain to enhance protein-protein interactions. However, this modification needs to be added along with SH3 domain (BBa_K4061110) and the scaffold.

Parts Number

Parts Name

Description

OmpF promoter

OmpF promoter will be activated under low intracellular osmolarity conditions. This part contains the negative regulator region, F4 at -360bp.

BBa_K4061002

eGFP

eGFP is a mutant form of GFP that has a higher fluorescence intensity and better photo-stability as compared to the native GFP. This particular eGFP sequence has been codon-optimized for usage in E.coli.

BBa_K4061020

Engineered EnvZ*

The engineered EnvZ contains the native EnvZ with the addition of SH3 domain on its C terminus and an auto-inhibition domain which is an SH3 ligand close to its kinase activity domain. The engineered EnvZ has to be used with the scaffold and the engineered OmpR.

BBa_K4061010

Engineered OmpR*

OmpR is a native regulatory protein in E.coli that facilitates the expression of the OmpF and OmpC genes. The engineered one is equipped with a leucine zipper. It is meant to work together with the engineered EnvZ and scaffold.

BBa_K4061015

Scaffold*

The scaffold contains a leucine zipper adapter and an SH3 ligand. The scaffold functions to increase the interaction of the engineered EnvZ and the engineered OmpR.

BBa_K4061045

mRFP1 antisense RNA

The mRFP1 antisense has been proven to downregulate mRFP1 (BBa_E1010) that is controlled under RBS (BBa_B0034) and OmpF promoter (BBa_K4061000).
Refer to our Results page to look at its downregulating ability.

BBa_K4061040

eGFP antisense RNA

We have previously demonstrated the downregulation of eGFP (BBa_K4061002) that is controlled under RBS (BBa_B0034) and OmpC promoter (BBa_R0082) with this eGFP antisense.
Refer to our Results page to look at its downregulating ability.

BBa_K4061041

eGFP antisense RNA(characterization)

This fragment is similar to BBa_K4061040 but this is optimised to regulate constitutively expressed eGFP. Only the -10 promoter region is changed. This was used for the characterisation of antisense RNA efficiency.

BBa_K4061046

mRFP1 antisense RNA (characterisation)

This fragment is similar to BBa_K4061045 but this is optimised to regulate constitutively expressed mRFP1. Only the -10 promoter region is changed. This was used for the characterisation of antisense RNA efficiency.

BBa_K4061110

SH3 domain*

This part is registered separately here so other teams could utilise this domain to enhance protein-protein interactions. However, this modification needs to be added along with the Leucine Zipper domain (BBa_K4061115) and the scaffold.

BBa_K4061115

Leucine Zipper domain*

This part is registered separately here so other teams could utilise this domain to enhance protein-protein interactions. However, this modification needs to be added along with SH3 domain (BBa_K4061110) and the scaffold.

Basic Parts

Full List of Our Parts