Team:Guelph/Parts

Here is a comprehensive list of all of the BioBricks we created this year!

BioBrick	Type	Name	Description	Length
BBa_K4058000	RNA	CLE18 sgRNA A for CRISPRa	A single guide RNA designed for use in a CRISPRa system. It targets a short region of CLE18’s promoter.	20
BBa_K4058001	RNA	CLE18 sgRNA B for CRISPRa	A single guide RNA designed for use in a CRISPRa system. It targets a short region of CLE18’s promoter.	20
BBa_K4058002	RNA	CLE18 sgRNA C for CRISPRa	A single guide RNA designed for use in a CRISPRa system. It targets a short region of CLE18’s promoter.	20
BBa_K4058003	RNA	CLE18 sgRNA D for CRISPRa	A single guide RNA designed for use in a CRISPRa system. It targets a short region of CLE18’s promoter.	20
BBa_K4058004	RNA	CLE18 sgRNA E for CRISPRa	A single guide RNA designed for use in a CRISPRa system. It targets a short region of CLE18’s promoter.	20
BBa_K4058005	RNA	ICE2 sgRNA A for CRISPRa	A single guide RNA designed for use in a CRISPRa system. It targets a short region of ICE2’s promoter.	20
BBa_K3189006	RNA	ICE2 sgRNA B for CRISPRa	A single guide RNA designed for use in a CRISPRa system. It targets a short region of ICE2’s promoter.	20
BBa_K4058007	RNA	ICE2 sgRNA C for CRISPRa	A sequence that allows the ribosome to bind and initiate protein translation.	20
BBa_K4058008	RNA	ICE2 sgRNA D for CRISPRa	A single guide RNA designed for use in a CRISPRa system. It targets a short region of ICE2’s promoter.	20
BBa_K4058009	RNA	ICE2 sgRNA E for CRISPRa	A single guide RNA designed for use in a CRISPRa system. It targets a short region of ICE2’s promoter.	20
BBa_K4058010	Plasmid	pHSN6A01 (CRISPRa plasmid)	This plasmid contains a dCas9 region fused to a VP64 transcriptional activation domain, which results in increased transcription from the targeted gene.	13023
BBa_K4058018	Composite	CLE18 sgRNA A in pHSN6A01	This plasmid contains a dCas9 region fused to a VP64 transcriptional activation domain. It also contains a sgRNA sequence which directs the CRISPRa system to bind to CLE18’s promoter.	13055
BBa_K4058019	Composite	CLE18 sgRNA B in pHSN6A01	This plasmid contains a dCas9 region fused to a VP64 transcriptional activation domain. It also contains a sgRNA sequence which directs the CRISPRa system to bind to CLE18’s promoter.	13055
BBa_K4058020	Composite	CLE18 sgRNA C in pHSN6A01	This plasmid contains a dCas9 region fused to a VP64 transcriptional activation domain. It also contains a sgRNA sequence which directs the CRISPRa system to bind to CLE18’s promoter.	13055
BBa_K4058021	Composite	CLE18 sgRNA D in pHSN6A01	This plasmid contains a dCas9 region fused to a VP64 transcriptional activation domain. It also contains a sgRNA sequence which directs the CRISPRa system to bind to CLE18’s promoter.	13055
BBa_K4058022	Composite	CLE18 sgRNA E in pHSN6A01	This plasmid contains a dCas9 region fused to a VP64 transcriptional activation domain. It also contains a sgRNA sequence which directs the CRISPRa system to bind to CLE18’s promoter.	13055
BBa_K4058023	Composite	ICE2 sgRNA A in pHSN6A01	This plasmid contains a dCas9 region fused to a VP64 transcriptional activation domain. It also contains a sgRNA sequence which directs the CRISPRa system to bind to ICE2’s promoter.	13055
BBa_K4058024	Composite	ICE2 sgRNA B in pHSN6A01	This plasmid contains a dCas9 region fused to a VP64 transcriptional activation domain. It also contains a sgRNA sequence which directs the CRISPRa system to bind to ICE2’s promoter.	13055
BBa_K4058025	Composite	ICE2 sgRNA C in pHSN6A01	This plasmid contains a dCas9 region fused to a VP64 transcriptional activation domain. It also contains a sgRNA sequence which directs the CRISPRa system to bind to ICE2’s promoter.	13055
BBa_K4058026	Composite	ICE2 sgRNA D in pHSN6A01	This plasmid contains a dCas9 region fused to a VP64 transcriptional activation domain. It also contains a sgRNA sequence which directs the CRISPRa system to bind to ICE2’s promoter.	13055
BBa_K4058027	Composite	ICE2 sgRNA E in pHSN6A01	This plasmid contains a dCas9 region fused to a VP64 transcriptional activation domain. It also contains a sgRNA sequence which directs the CRISPRa system to bind to ICE2’s promoter.	13055
BBa_K4058016	Composite	CLE18 multiplex gRNA (CLE18 sgRNAs A, B, C, D, E)	This construct contains our five CLE18 sgRNAs.	2910
BBa_K4058017	Composite	ICE2 multiplex gRNA (CLE18 sgRNAs A, B, C, D, E)	This construct contains our five ICE2 sgRNAs.	2910
BBa_K4058033	Composite	CLE18 multiplex gRNA in pHSN6A01	This plasmid contains a dCas9 region fused to a VP64 transcriptional activation domain. It also contains a multiplexed gRNA sequence which directs the CRISPRa system to bind to multiple locations in CLE18’s promoter.	15965
BBa_K4058034	Composite	ICE2 multiplex gRNA in pHSN6A01	This plasmid contains a dCas9 region fused to a VP64 transcriptional activation domain. It also contains a multiplexed gRNA sequence which directs the CRISPRa system to bind to multiple locations in ICE2’s promoter.	15965
BBa_K4058030	Composite	AlcR-pHSN6A01	The pHSN6A01 plasmid with the CaMV35s constitutive promoter in front of dCas9 substituted for the AlcR ethanol inducible promoter.	16935
BBa_K4058031	Composite	CLE18 multiplex gRNA in AlcR-pHSN6A0	This plasmid contains a dCas9 region fused to a VP64 transcriptional activation domain, which is under the ethanol-inducible control of the AlcR promoter. It also contains a multiplexed gRNA sequence which directs the CRISPRa system to bind to multiple locations in CLE18’s promoter.	19845
BBa_K4058032	Composite	ICE2 multiplex gRNA in AlcR-pHSN6A0	This plasmid contains a dCas9 region fused to a VP64 transcriptional activation domain, which is under the ethanol-inducible control of the AlcR promoter. It also contains a multiplexed gRNA sequence which directs the CRISPRa system to bind to multiple locations in ICE2’s promoter.	19845
BBa_K4058031	Composite	AlcR ethanol inducible promoter	The AlcR promoter has active transcription when ethanol is present but is otherwise inactive	3912