Team:GA State SW Jiaotong/Model
Overview
An excellent model can provide reliable theoretical support for project design and selection. In this project, the growth competition model demonstrated that Bacillus Subtilis WB600 had no evil competition effect on the microenvironment of scalp bacteria, which provided theoretical support for the safety of the project scheme.
In addition, the dynamic simulation model analyzed the variation of Hok-protein concentration expressed by toxin-antitoxin (Hok/Sok) mechanism under different regulatory mechanisms, providing effective theoretical support for the design and selection of suicide switches. All these make the whole project carried out scientifically and effectively.
Strain growth competition model
The growth characteristics of Bacillus Subtilis WB600, M. restricta and S. epidermidis co-culture conditions were analyzed by Logistic modeling of microbial interactions. It was proved that Bacillus Subtilis WB600 had no significant evil competition with M. restricta and S. epidermidis. Similarly, this indicated that Bacillus Subtilis WB600 had little damage to the microflora balance in the scalp microenvironment. This method can be used to assess the level of competition between any other flora.
A dynamic simulation model of the toxin-antitoxin (Hok/Sok) mechanism
According to the molecular mechanism of toxin-antitoxin mechanism (Hok/Sok), the dynamic equations of toxin-antitoxin mechanism (Hok/Sok) under common and dual-modulated were established. This system demonstrated the effectiveness and controllability of the dual-modulated toxin-antitoxin (Hok/Sok) mechanism in suicide control. Similarly, the toxin-antitoxin mechanism dynamics model can also provide a reference for other similar system dynamics models.
