Team:GA State SW Jiaotong/Attributions
Hnoss
Hnoss is a big family, distance doesn’t matter, though some of us are staying in China and some of us are staying in the US, we are still writing a few more words of our story every day. We want the story to be an adventure and that's made all the difference. Thank you to everyone for hard-working throughout this program and Hnoss will continue our story in the future.
WET LAB
Plasmid construction (pPICZaA + Gas6;pNW33N + Gas6 + suicide plasmid;PNW33N+EGF) |
SWJTU iGEM: Ningxin Zhu, Xiaoyi Feng,Jingyi Xu,Shiqi Huang, Long Xie,Yang Hu, Xinran Ji,Binyuan Pan, Yuwei Che,Yangying Chen,Shengnan Suo,Yu Ji Zhihao Zhang, Wanjun Luo, Aliyah Bujung,Jessica Dee, Deva Balaji, Siobhanna Brown, Pamela Issa, Samadhi Senanayake and Varun Senthil |
Suicide system construction |
SWJTU iGEM : Ningxin Zhu, Xiaoyi Feng,Liyuan Liu,Jingyi Xu,Shiqi Huang, Long Xie,Yang Hu, Xinran Ji,Binyuan Pan, Yuwei Che GSU iGEM: Zhihao Zhang, Wanjun Luo, Aliyah Bujung,Jessica Dee, Deva Balaji, Siobhanna Brown, Pamela Issa, Samadhi Senanayake and Varun Senthil |
Pichia pastoris operation |
SWJTU iGEM: Ningxin Zhu, Xiaoyi Feng,Jingyi Xu,Shiqi Huang, Long Xie,Yang Hu, Xinran Ji,Binyuan Pan, Yuwei Che,Xucheng Zhang,Jiatong Li,Chengwen Chu GSU iGEM: Zhihao Zhang, Wanjun Luo, Aliyah Bujung,Jessica Dee, Deva Balaji, Siobhanna Brown, Pamela Issa, Samadhi Senanayake and Varun Senthil |
Transformation and expression of pichia pastoris |
SWJTU iGEM: Ningxin Zhu, Xiaoyi Feng,Jingyi Xu,Shiqi Huang, Long Xie,Yang Hu, Xinran Ji,Binyuan Pan, Yuwei Che,Xucheng Zhang,Jiatong Li,Chengwen Chu GSU iGEM: Zhihao Zhang, Wanjun Luo, Aliyah Bujung,Jessica Dee, Deva Balaji, Siobhanna Brown, Pamela Issa, Samadhi Senanayake and Varun Senthil |
E.coli operation |
SWJTU iGEM: Ningxin Zhu, Xiaoyi Feng,Jingyi Xu,Shiqi Huang, Long Xie,Yang Hu, Xinran Ji,Binyuan Pan, Yuwei Che GSU iGEM: Zhihao Zhang, Wanjun Luo, Aliyah Bujung,Jessica Dee, Deva Balaji, Siobhanna Brown, Pamela Issa, Samadhi Senanayake and Varun Senthil |
Transformation and expression of E.coli |
SWJTU iGEM: Ningxin Zhu, Xiaoyi Feng,Jingyi Xu,Shiqi Huang, Long Xie,Yang Hu, Xinran Ji,Binyuan Pan, Yuwei Che,Yangying Chen,Shengnan Suo GSU iGEM: Zhihao Zhang, Wanjun Luo, Aliyah Bujung,Jessica Dee, Deva Balaji, Siobhanna Brown, Pamela Issa, Samadhi Senanayake and Varun Senthil |
Protein purification |
SWJTU iGEM: Ningxin Zhu, Xiaoyi Feng,Jingyi Xu,Shiqi Huang, Long Xie,Yang Hu, Xinran Ji,Binyuan Pan, Yuwei Che GSU iGEM: Zhihao Zhang, Wanjun Luo, Aliyah Bujung,Jessica Dee, Deva Balaji, Siobhanna Brown, Pamela Issa, Samadhi Senanayake and Varun Senthil |
Plasmid construction of Dino III GUS plasmid |
GSU iGEM: Zhihao Zhang, Wanjun Luo, Aliyah Bujung,Jessica Dee, Deva Balaji, Siobhanna Brown, Pamela Issa, Samadhi Senanayake and Varun Senthil |
Transformation of DinoIII-GFP into E.coli |
GSU iGEM: Zhihao Zhang, Wanjun Luo, Aliyah Bujung,Jessica Dee, Deva Balaji, Siobhanna Brown, Pamela Issa, Samadhi Senanayake and Varun Senthil |
Transformation of PBI121-GUS into E.coli |
GSU iGEM: Zhihao Zhang, Wanjun Luo, Aliyah Bujung,Jessica Dee, Deva Balaji, Siobhanna Brown, Pamela Issa, Samadhi Senanayake and Varun Senthil |
Transformation of DinoIII-GUS into E.coli |
GSU iGEM: Zhihao Zhang, Wanjun Luo, Aliyah Bujung,Jessica Dee, Deva Balaji, Siobhanna Brown, Pamela Issa, Samadhi Senanayake and Varun Senthil |
Transformation of DinoIII-GUS into Agrobacterium Tumefacien |
GSU iGEM: Zhihao Zhang, Wanjun Luo, Aliyah Bujung,Jessica Dee, Deva Balaji, Siobhanna Brown, Pamela Issa, Samadhi Senanayake and Varun Senthil |
Codon-Optimization of GUS |
GSU iGEM: Zhihao Zhang, Wanjun Luo, Aliyah Bujung,Jessica Dee, Deva Balaji, Siobhanna Brown, Pamela Issa, Samadhi Senanayake and Varun Senthil |
Modeling
Guodong Tan, Shiqi Huang, Shufei Luo, Xucheng Zhang, Yang Chen
OUTREACH
Wanjun Luo, Deva Balaji, Yang Hu, Ningxin Zhu
VISUAL SYSTEM
Siobhanna Brown, Yuwei Che, Binyuan Pan,Houyi He, Zhaojun Wu,Yu Ji, Shihan Zhang, Xinran Ren
PRESENTATION & PROMOTION VIDEO
Shiqi Huang, Xinran Ji, Yu Ji, Shengnan Suo
HUMAN PRACTICE & WORDS
Yang Hu, Jingyi Xu, Xinran Ji, Zhaojun Wu, Haoran Li, Xinran Ren, Binyuan Pan, Siyu Ji
Special thanks
Special thanks to Dr. Matthew Brewer and Dr.Xinhe Huang who participated in the whole project and solved our problems in time.
Special thanks to Dr.Hai Liao, Dr.Jiayu Zhou, and Jessica Siemer who provided valuable suggestions for the Hnoss.
Special thanks to Dr. Hyuk Kyu Seoh and Dr.Pc Tai who gave a helping hand when our experiments failed.
Special thanks to these collaboration schools who provides help and suggestions for the Hnoss.
Partnership and collaboration
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Shanghai Tech University(Partnership) brought up the idea of the suicide system and how to implement it to our project |
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NPU(Partnership) brought up the protocol for electro-transformation of Bacillus Subtilis, shared with us some tips for making agarose gel electrophoresis and colony PCR |
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Tongji_China communicated with Hnoss for the Questionnaire, suicide system, and modeling |
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SZ_SHD communicated with Hnoss for product research, technology, and human practice |
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SJTang communicated with Hnoss for modeling,human practice, and wiki |
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Lambert High school provided feedback regarding our project while we tested their lyophilizer |
