Team:BUCT-China/Contribution

Part Ⅰ
Part Ⅱ
Part Ⅲ
Part Ⅳ

Contribution

Ⅰ.Promoting iGEM and synthetic biology

1. Communication(https://2021.igem.org/Team:BUCT-China/Human_Practices)
This year we participant in four meeting or conferences as a team:
        1. iGEM 2021 Beijing District meeting on 04/18/2021
        2. 2021 China iGEM Online Meetup on 05/29/2021
        3. iGEM Northern China Meetup 2021 on 06/20/2021
        4. CCiC (Conference of China iGEMer Community) on 08/27/2021

2. Scientific education(https://2021.igem.org/Team:BUCT-China/Communication)
We conduct our scientific education through online live on the Bilibili, Wechat public account and collaborating with igemers from other teams to popularized the synthetic biology and discuss questions regarding to the synthetic biology like ethics and artificial meat.

3. Promoting Synthetic biology ethics
Through CCiC (Conference of China iGEMer Community) on 08/27/2021, we meet with Yuhan Bao, who is a PHD candidate in School of Public Policy & Management in Tsinghua University and his study is focusing on the policy and risk management of synthetic biology. We set up an online meeting with Mr. Yuhan Bao and discussed various topics about synthetic biology, like what is the difference between synthetic biology and traditional molecular biology, what is the boundary or is there a boundary of the field of synthetic biology, whether the safety protocol in Bio-laboratory is going to change to prevent unknown risk when new technology is established in synthetic biology, what is the current problem about synthetic biology studies and extra.

Ⅱ.Design new part

The COVID-19 still pose an adverse impact on this year’s research, we persist in design our part through some documents and proved the feasibility of our parts through some verification experiments. See the links in parts for details：
http://parts.igem.org/Part:BBa_K3742000
http://parts.igem.org/Part:BBa_K3742001
http://parts.igem.org/Part:BBa_K3742002

Ⅲ.Experiment design and verification

We have designed the whole project into two section to verify our idea of cultured meat. In first section, we construct several metabolic pathways in E.coli to synthesize PHFA (Poly Hydroxy Fatty Acid) from glucose and we also use E.coli to synthesize functional collagen. Although the whole metabolic pathways are not in one cell and the degree of polymerization is not very high, this provides us with a good idea in application in cultured meat. In the second part, we utilized the PHFA and collagen as raw material in Bio-3D printing scaffold, and then mix chicken cells with materials to make cultured meat. See the links for details：
https://2021.igem.org/Team:BUCT-China/Design
https://2021.igem.org/Team:BUCT-China/Engineering
https://2021.igem.org/Team:BUCT-China/Proof_Of_Concept

Ⅳ.Bio-3D printing

3D printing is not only for mechanical usage, but also for tissue engineering. Using specific material and Bio-3D printer, we explore the characteristic of our 3D printed scaffold with documentation of G-code by software Slic3r and the result of 3D printed scaffold is in this link:https://2021.igem.org/Team:BUCT-China/Proof_Of_Concept

The Bio-3D printer that we use is from bio-company CELLINK

Print document design in software Slic3r:
The initial interface

Cut out the total height

Setting print Parameters

Output the G code file

Visualize the G code