Time we had spent
Week1: Brainstorming to focus on recycled paper
Week2: Paper work to focus on two main proteins.
Week3: Due to the COVID-19 epidemic, we could not receive the kit from hq, so we asked bio-company to synthesis our gene this year and today we receive plasmids and solution from company. We begin to inoculate and culture in liquid medium in order to preserve them in glycerol the next week.
Week4: Transfect the plasmid into E.coli BL21(DE3). We also use glycerol to preserve the bacteria. So this week we focus on four projects:
1. plasmid extraction
2. transformation
3. agarose gel electrophoresis
4. glycerol preservation
Week5: We have done the following things this week:
1. We help other teams with their project, especially KEYSTONE iGEM team. We mentored each other during the molecular cloning project.
2.We have prepared the culture medium, not only the cultivate culture medium, but the expression induction medium.
3.We keep on trying to transfect the plasmid, but it haven’t succeeded up to now.
Week6:
1.We are still trying to use E.coli to express our protein. We have succeeded once. We think maybe we have found the proper cultivating condition for E.coli to express our protein.
2.We asked KEYSTONE iGEM team again for their project.
Week7:
1.We use western blot to test our proteins. The antibody we use is to test His-tag, because both C-terminal and N-terminal of our protein has a 6xHis tag. The result is great, we truly produced some proteins.
2.We express more protein for our next experiment.>
Week8:
1.After we get more proteins, we use the proteins to test its function.
2.We supposed to make some recycled paper ourselves. After reading more papers and study from our teacher, we learned how to make recycled paper in our lab.
3.So we designed experiments to test whether add our protein can change the phenotype of recycled paper.
4.And we set several indicators to test whether our protein can improve the quality of recycled paper.
