Antimicrobial Peptide Improvement

Our team utilized Machine Learning to improve an AMP to produce different variants. Make sure to check out the github link within the Contribution session, https://2021.igem.org/Team:HKIS/Contribution link for more details for the machine learning! We have also 3D printed an awesome incubator hardware that worked fantastically with super saturated sodium acetate heat packs.

Antimicrobial peptides (AMPs) are a special kind of peptide that is used as an innate immune defense mechanism for all forms of life, against invading bacteria, acting as a Host Defence Peptide (HDP). Many of them accomplish this purpose by disrupting the cellular membranes of the bacteria in question and thereby killing them. The shape of the AMP is directly related to its ability to perform its function, as it affects its binding affinity to its target. The most common varieties of AMPs found in nature that disrupt biological membranes have an α-helical structure, forming a coil-like shape. They are also amphipathic, meaning that they have both hydrophobic and hydrophilic regions, their amphipathicity is extremely important to their ability

The template of an AMP (the specific amino acid sequence) is of extreme importance in determining its shape and consequently its efficacy in its function. The specific pattern of folding caused by the various intermolecular interactions between amino acids in the sequence is crucial for it to be able to perform its function well. Moreover, the different natures of the cell membranes of different bacteria necessitate that the template sequence of the AMP is perfectly matched against a specific bacteria. Vibrio bacteria specifically have a plethora of defense mechanisms against many different kinds of AMPs, meaning that a modification of the template sequence on our part would be required for maximum efficacy.

For our team, Anti-Microbial Peptides had a special significance, in that they offered a unique and effective method of treating oyster populations that were contaminated with Vibrio bacteria, while not ruining the oysters for future sale and consumption, a radical departure from previous methods of treatment. Because of this, we investigated their mechanism against colonizing bacteria, as well as their structure and method of production, and their possible use against Vibrio bacteria.

Antimicrobial peptides (AMPs) are a special kind of peptide used as an innate immune defense mechanism for all forms of life against invading bacteria. Many of them defend against invading bacteria by disrupting the cellular membranes of the bacteria in question and thereby killing them. The shape of the AMP is directly related to its ability to perform its function, as it affects its binding affinity to its target. The most common varieties of AMPs found in nature that accomplish this cellular purpose have either an α-helical structure, forming a coil-like shape, or a β-sheet structure, forming overlapping blanket structures. They are also amphipathic. Amphipathicity is incredibly important as antimicrobial peptides would not be able to kill bacteria without being able to permeate through the membrane.

Our Improved Part: 41012 to 41014