Difference between revisions of "Team:Shanghai United/Basic Parts"

(Created page with "<html> <head> <meta charset="utf-8"> <meta name="viewport" content="width=device-width, initial-scale=1, maximum-scale=1"> <title>非模块化方式使用layui</title>...")
 
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<html>
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<html lang="en">
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<head>
 
<head>
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<meta charset="utf-8">
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        <meta charset="UTF-8">
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<meta name="viewport" content="width=device-width, initial-scale=1, maximum-scale=1">
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        <meta http-equiv="X-UA-Compatible" content="IE=edge">
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<title>非模块化方式使用layui</title>
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        <meta name="viewport" content="width=device-width, initial-scale=1.0">
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<link rel="stylesheet" href="https://2021.igem.org/Team:Shanghai_United/Template:CSS?action=raw&amp;ctype=text/css">
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        <title>Document</title>
 +
        <link rel="stylesheet"
 +
                href="https://2021.igem.org/Team:Shanghai_United/Template:CSS?action=raw&amp;ctype=text/css">
 +
        <script src="https://code.jquery.com/jquery-3.1.1.min.js"></script>
 +
        <script src="https://stackpath.bootstrapcdn.com/bootstrap/3.4.1/js/bootstrap.min.js"
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                crossorigin="anonymous"></script>
  
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<style type="text/css">
 
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#sideMenu,
 
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#top_title,
 
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.patrollink,
 
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#firstHeading,
 
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#home_logo,
 
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#sideMenu {
 
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display: none;
 
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}
 
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#content {
 
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padding: 0px;
 
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width: 100%;
 
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margin-top: -7px;
 
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margin-left: 0px;
 
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border: none;
 
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}
 
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body,
 
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html {
 
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background-color: white;
 
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width: 100%;
 
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height: 100%;
 
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}
 
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#bodyContent h1,
 
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#bodyContent h2,
 
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#bodyContent h3,
 
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#bodyContent h4,
 
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#bodyContent h5 {
 
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margin-bottom: 0px;
 
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}
 
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#bodyContent a[href ^="https://"],
 
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.link-https {
 
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padding-right: 0px;
 
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}
 
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</style>
 
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<title>Entprenuership</title>
 
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</head>
 
  
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<style type="text/css">
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</head>
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.layui-nav .layui-nav-item a {
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<style type="text/css">
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color: black;
+
        #sideMenu,
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}
+
        #top_title,
−
+
        .patrollink,
−
.layui-nav .layui-nav-item a:hover {
+
        #firstHeading,
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color: black;
+
        #home_logo,
−
text-decoration: none;
+
        #sideMenu {
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}
+
                display: none;
−
+
        }
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#bodyContent a[href ^="https://"],
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−
.link-https {
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−
padding-right: 35px;
+
−
}
+
−
.layui-nav * {
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−
    font-size: 18px;
+
−
}
+
−
.carousel-indicators {
+
  
−
    width: 30%;
+
        #content {
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left: 33%;
+
                padding: 0px;
−
+
                width: 100%;
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</style>
+
                margin-top: -7px;
 +
                margin-left: 0px;
 +
                border: none;
 +
        }
  
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<body>
+
        body,
 +
        html {
 +
                background-color: white;
 +
                width: 100%;
 +
                height: 100%;
 +
        }
  
−
<div>
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        #bodyContent h1,
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<!--导航条固定部分-->
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        #bodyContent h2,
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<div style="height: 255px;width: 100%;">
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        #bodyContent h3,
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<div style="text-align: center;">
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        #bodyContent h4,
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<img src="https://static.igem.org/mediawiki/2020/0/03/T--Xiamen_city--logo.jpg" style="width: 80px; height: 80px;margin-top: 20px;" />
+
        #bodyContent h5 {
−
</div>
+
                margin-bottom: 0px;
 +
        }
  
−
<div style="text-align: center; margin-top: 20px;">
+
        #bodyContent a[href ^="https://"],
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<span style="font-size: 30px;"><b>Nap Tuner</b></span>
+
        .link-https {
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</div>
+
                padding-right: 0px;
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<hr />
+
        }
 +
</style>
 +
<style>
 +
        html {
 +
                width: 100%;
 +
                height: 100%;
 +
        }
  
−
<ul class="layui-nav navbar navbar-fixed-top text-center" lay-filter="" style="background-color:white">
+
        body {
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<li class="layui-nav-item" style="text-align: center;">
+
                min-width: 1920px;
−
<a href="https://2021.igem.org/Team:Shanghai_United" style="text-align: center;">Home</a>
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                overflow: scroll;
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</li>
+
                margin: 0 auto;
 +
        }
  
−
<li class="layui-nav-item" style="text-align: center;">
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        .layui-nav .layui-nav-item a {
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<a href="javascript:;">Project</a>
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                color: black;
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<dl class="layui-nav-child">
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                font-size: 17px;
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<!-- 二级菜单 -->
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        }
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<dd>
+
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<a href="https://2021.igem.org/Team:Shanghai_United/Description">Description</a>
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</dd>
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<dd>
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<a href="https://2021.igem.org/Team:Shanghai_United/Design">Design</a>
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</dd>
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<dd>
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<a href="https://2021.igem.org/Team:Shanghai_United/Experiment">Experiments</a>
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</dd>
+
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<dd>
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<a href="https://2021.igem.org/Team:Shanghai_United/Notebook">Notebook</a>
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</dd>
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<dd>
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<a href="https://2021.igem.org/Team:Shanghai_United/Result">Result</a>
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</dd>
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<dd>
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<a href="https://2021.igem.org/Team:Shanghai_United/Safety">Safety</a>
+
−
</dd>
+
  
−
</dl>
+
        .layui-nav .layui-nav-item a:hover {
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</li>
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                color: black;
 +
                text-decoration: none;
 +
        }
  
−
<li class="layui-nav-item" style="text-align: center;">
+
        #bodyContent a[href ^="https://"],
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<a href="https://2021.igem.org/Team:Shanghai_United/Contribution">Contribution</a>
+
        .link-https {
−
</li>
+
                padding-right: 35px;
 +
        }
  
−
<li class="layui-nav-item">
+
        .layui-nav * {
−
<a href="https://2021.igem.org/Team:Shanghai_United/Engineering">Engineering</a>
+
                font-size: 18px;
−
</li>
+
        }
−
<li class="layui-nav-item">
+
−
<a href="https://2021.igem.org/Team:Shanghai_United/Implementation">Implementation</a>
+
−
</li>
+
−
<li class="layui-nav-item" style="text-align: center;">
+
−
<a href="javascript:;">Parts</a>
+
−
<dl class="layui-nav-child">
+
−
<!-- 二级菜单 -->
+
−
<dd>
+
−
<a href="https://2021.igem.org/Team:Shanghai_United/Basic Parts">Basic Parts</a>
+
−
</dd>
+
−
<dd>
+
−
<a href="https://2021.igem.org/Team:Shanghai_United/Composite Parts">Composite Parts</a>
+
−
</dd>
+
−
<dd>
+
−
<a href="https://2021.igem.org/Team:Shanghai_United/Parts Collection">Parts Collection</a>
+
−
</dd>
+
  
−
</dl>
+
        .carousel-indicators {
−
</li>
+
−
<li class="layui-nav-item" style="text-align: center;">
+
−
<a href="https://2021.igem.org/Team:Shanghai_United/Model">Model</a>
+
−
</li>
+
−
<li class="layui-nav-item">
+
−
<a href="javascript:;">Human Practice</a>
+
−
<dl class="layui-nav-child">
+
−
<!-- 二级菜单 -->
+
  
−
<dd>
+
                width: 30%;
−
<a href="https://2021.igem.org/Team:Shanghai_United/Human_Practices">Integrated Human Practice</a>
+
                left: 33%;
−
</dd>
+
        }
−
<dd>
+
−
<a href="https://2021.igem.org/Team:Shanghai_United/Fundraising">Fundraising</a>
+
−
</dd>
+
−
<dd>
+
−
<a href="https://2021.igem.org/Team:Shanghai_United/Entrepreneurship">Entrepreneurship</a>
+
−
</dd>
+
−
</dl>
+
−
</li>
+
  
−
<li class="layui-nav-item" style="text-align: center;">
+
        .layui-carousel:hover .layui-carousel-arrow {
−
<a href="javascript:;">Team</a>
+
                display: none;
−
<dl class="layui-nav-child">
+
        }
−
<!-- 二级菜单 -->
+
−
<dd>
+
−
<a href="https://2021.igem.org/Team:Shanghai_United/Team">Team Member</a>
+
−
</dd>
+
−
<dd>
+
−
<a href="https://2021.igem.org/Team:Shanghai_United/Collaborations">Collaborations</a>
+
−
</dd>
+
−
<dd>
+
−
<a href="https://2021.igem.org/Team:Shanghai_United/Attributions">Attributions</a>
+
−
</dd>
+
  
−
</dl>
+
        .layui-carousel {
−
</li>
+
                height: 1080px !important;
−
</ul>
+
        }
 +
</style>
  
−
</div>
+
<body>
−
<!--导航条固定部分结束-->
+
        <div style="height: 255px;width: 100%;display: flex;justify-content: space-around;">
 +
                <img src="https://static.igem.org/mediawiki/2021/2/27/T--Shanghai_United--homepage01.png"
 +
                        style="width: 173px; height: 89px;margin-right: 271px;margin-left: 120px;margin-top: 80px;display: block;" />
 +
                <ul class="layui-nav navbar navbar-fixed-top text-center" lay-filter=""
 +
                        style="background-color:white;height: 255px;line-height: 255px;">
  
 +
                        <li class="layui-nav-item" style="text-align: center;">
 +
                                <a href="https://2021.igem.org/Team:Shanghai_United"
 +
                                        style="text-align: center;">Home</a>
 +
                        </li>
  
 +
                        <li class="layui-nav-item" style="text-align: center;">
 +
                                <a href="javascript:;">Project</a>
 +
                                <dl class="layui-nav-child">
 +
                                        <!-- 二级菜单 -->
 +
                                        <dd>
 +
                                                <a
 +
                                                        href="https://2021.igem.org/Team:Shanghai_United/Description">Description</a>
 +
                                        </dd>
 +
                                        <dd>
 +
                                                <a href="https://2021.igem.org/Team:Shanghai_United/Proof">Proof
 +
                                                        of Concept</a>
 +
                                        </dd>
 +
                                        <dd>
 +
                                                <a
 +
                                                        href="https://2021.igem.org/Team:Shanghai_United/Experiments">Experiments</a>
 +
                                        </dd>
 +
                                        <dd>
 +
                                                <a
 +
                                                        href="https://2021.igem.org/Team:Shanghai_United/Notebook">Notebook</a>
 +
                                        </dd>
 +
                                        <dd>
 +
                                                <a href="https://2021.igem.org/Team:Shanghai_United/Results">Results</a>
 +
                                        </dd>
 +
                                        <dd>
 +
                                                <a href="https://2021.igem.org/Team:Shanghai_United/Safety">Safety</a>
 +
                                        </dd>
  
−
<div id="myCarousel" class="carousel slide" data-ride="carousel" data-interval="2000">
+
                                </dl>
−
    <!-- 轮播（Carousel）指标 -->
+
                        </li>
−
    <ol class="carousel-indicators">
+
                        <li class="layui-nav-item" style="text-align: center;">
−
        <li data-target="#myCarousel" data-slide-to="0" class="active"></li>
+
                                <a href="javascript:;">Parts</a>
−
        <li data-target="#myCarousel" data-slide-to="1"></li>
+
                                <dl class="layui-nav-child">
−
        <li data-target="#myCarousel" data-slide-to="2"></li>
+
                                        <!-- 二级菜单 -->
−
    </ol>  
+
                                        <dd>
−
    <!-- 轮播（Carousel）项目 -->
+
                                                <a
−
    <div class="carousel-inner">
+
                                                        href="https://2021.igem.org/Team:Shanghai_United/Basic Parts">Engineering</a>
−
        <div class="item active">
+
                                        </dd>
−
            <img src="https://static.igem.org/mediawiki/2020/4/42/T--Xiamen_city--homepage01.jpg" alt="First slide">
+
                                        <dd>
−
        </div>
+
                                                <a href="https://2021.igem.org/Team:Shanghai_United/Parts Collection">Parts
−
        <div class="item">
+
                                                        Collection</a>
−
            <img src="https://static.igem.org/mediawiki/2020/4/4d/T--Xiamen_city--homepage02.jpg" alt="Second slide">
+
                                        </dd>
−
        </div>
+
−
        <div class="item">
+
−
            <img src="https://static.igem.org/mediawiki/2020/8/85/T--Xiamen_city--homepage03.jpg" alt="Third slide">
+
−
        </div>
+
−
    </div>
+
−
    <!-- 轮播（Carousel）导航 -->
+
−
    <a class="carousel-control left" href="#myCarousel"
+
−
      data-slide="prev"> <span _ngcontent-c3="" aria-hidden="true" class=""></span></a>
+
−
    <a class="carousel-control right" href="#myCarousel"
+
−
      data-slide="next">&#8250;</a>
+
−
</div>
+
  
 +
                                </dl>
 +
                        </li>
 +
                        <li class="layui-nav-item">
 +
                                <a href="javascript:;">Human Practice</a>
 +
                                <dl class="layui-nav-child">
 +
                                        <!-- 二级菜单 -->
 +
                                        <dd>
 +
                                                <a
 +
                                                        href="https://2021.igem.org/Team:Shanghai_United/Fundraising">Fundraising</a>
 +
                                        </dd>
 +
                                        <dd>
 +
                                                <a
 +
                                                        href="https://2021.igem.org/Team:Shanghai_United/Communication">Communication</a>
 +
                                        </dd>
 +
                                        <dd>
 +
                                                <a
 +
                                                        href="https://2021.igem.org/Team:Shanghai_United/Integrated_Human_Practices">Integrated Human Practices</a>
 +
                                        </dd>
 +
                                </dl>
 +
                        </li>
 +
                        <li class="layui-nav-item" style="text-align: center;">
 +
                                <a href="https://2021.igem.org/Team:Shanghai_United/Implementation">Implementation</a>
 +
                        </li>
  
 +
                        <li class="layui-nav-item" style="text-align: center;">
 +
                                <a
 +
                                        href="https://2021.igem.org/Team:Shanghai_United/Entrepreneurship">Entrepreneurship</a>
 +
                        </li>
  
 +
                        <li class="layui-nav-item" style="text-align: center;">
 +
                                <a href="https://2021.igem.org/Team:Shanghai_United/Model">Model</a>
 +
                        </li>
  
−
<div style="background-color: #c3a77a;color: white; padding: 5%;">
 
−
<p style="margin:0pt; color:white;orphans:0; text-align:justify; widows:0"><span style="font-family:'Times New Roman'; font-size:18pt; font-weight:bold">Abstract</span><br /><br /><span style="color:white;font-family:'Times New Roman'; font-size:14pt">Our team is to solve the health damage caused by staying up late. Lacking sleep will lead to the accumulation of Reactive oxygen species</span><span style="font-family:等线; font-size:14pt">（</span><span style="font-family:'Times New Roman'; font-size:14pt">ROS</span><span style="font-family:等线; font-size:14pt">）</span><span style="font-family:'Times New Roman'; font-size:14pt">in the intestine, which will damage human tissues, lead to the aging of the human body, shorten life span, induce chronic diseases, and even induce sudden death. Superoxide dismutase</span><span style="font-family:等线; font-size:14pt">（</span><span style="font-family:'Times New Roman'; font-size:14pt">SOD</span><span style="font-family:等线; font-size:14pt">）</span><span style="font-family:'Times New Roman'; font-size:14pt">, on the other hand, is an antioxidant enzyme that exists in the body. It can help to eliminate the excess ROS in a timely manner, reduce the accumulation of ROS and the production of oxidation reaction, and play a role in preventing the damage that ROS brings. Our goal is to create an engineered probiotic that can naturally produce SOD in the way of synthetic biology, then to produce a probiotics drink product that allows the probiotics to enter the intestinal tract by taking it, so as to eliminate the excessive ROS produced by staying up late.</span></p>
 
−
 
−
 
  
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</div>
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                        <li class="layui-nav-item" style="text-align: center;">
 +
                                <a href="javascript:;">Team</a>
 +
                                <dl class="layui-nav-child">
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                                        <!-- 二级菜单 -->
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                                        <dd>
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                                                <a href="https://2021.igem.org/Team:Shanghai_United/Team">Team
 +
                                                        Member</a>
 +
                                        </dd>
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                                        <dd>
 +
                                                <a
 +
                                                        href="https://2021.igem.org/Team:Shanghai_United/Collaborations">Collaborations</a>
 +
                                        </dd>
 +
                                        <dd>
 +
                                                <a
 +
                                                        href="https://2021.igem.org/Team:Shanghai_United/Attributions">Attributions</a>
 +
                                        </dd>
 +
                                </dl>
 +
                        </li>
 +
                </ul>
  
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<div style="background-color: #a6b2d8;color: black; padding: 5%;">
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        </div>
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<p style="font-size:14pt; line-height:115%; margin:0pt 0pt 10pt; orphans:0; text-align:justify; widows:0"><span style="font-family:'Times New Roman'; font-size:14pt">&#xa0;</span></p>
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<p style="font-size:18pt; line-height:115%; margin:0pt 0pt 10pt; orphans:0; text-align:justify; widows:0"><span style="font-family:'Times New Roman'; font-size:18pt; font-weight:bold">Solving !</span></p>
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<p style="font-size:14pt; line-height:115%; margin:0pt 0pt 10pt; orphans:0; text-align:justify; widows:0"><span style="font-family:'Times New Roman'; font-size:14pt">Our team is to solve the health damage caused by staying up late.</span></p>
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<p style="font-size:14pt; line-height:115%; margin:0pt 0pt 10pt; orphans:0; text-align:justify; widows:0"><span style="font-family:'Times New Roman'; font-size:14pt">&#xa0;</span></p>
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<p style="font-size:18pt; line-height:115%; margin:0pt 0pt 10pt; orphans:0; text-align:justify; widows:0"><span style="font-family:'Times New Roman'; font-size:18pt; font-weight:bold">Do you know ?</span></p>
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<p style="font-size:14pt; line-height:115%; margin:0pt 0pt 10pt; orphans:0; text-align:justify; widows:0"><span style="font-family:'Times New Roman'; font-size:14pt">Lacking of sleep will lead to the accumulation of ROS in the intestine, which will damage human tissues, lead to the aging of human body, shorten life span, induce chronic diseases, and even induce to sudden death.</span></p>
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<p style="font-size:14pt; line-height:115%; margin:0pt 0pt 10pt; orphans:0; text-align:justify; widows:0"><span style="font-family:'Times New Roman'; font-size:14pt">&#xa0;</span></p>
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<p style="font-size:14pt; line-height:115%; margin:0pt 0pt 10pt; orphans:0; text-align:justify; widows:0"><span style="font-family:'Times New Roman'; font-size:14pt">It can help to eliminate the excess ROS in a timely manner, reduce the accumulation of ROS and the production of oxidation reaction, and play a role in preventing the damage that ROS brings.</span></p>
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<p style="font-size:14pt; line-height:115%; margin:0pt 0pt 10pt; orphans:0; text-align:justify; widows:0"><span style="font-family:'Times New Roman'; font-size:14pt">&#xa0;</span></p>
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<p style="font-size:18pt; line-height:115%; margin:0pt 0pt 10pt; orphans:0; text-align:justify; widows:0"><span style="font-family:'Times New Roman'; font-size:18pt; font-weight:bold">Goal ?</span></p>
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<p style="font-size:14pt; line-height:115%; margin:0pt 0pt 10pt; orphans:0; text-align:justify; widows:0"><span style="font-family:'Times New Roman'; font-size:14pt">Our goal is to create an engineered probiotics that can naturally produce SOD in the way of synthetic biology, then to produce a probiotics drink product that allows the probiotics to enter the intestinal tract by taking it, so as to eliminate the excessive ROS produced by staying up late.</span></p>
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<p style="font-size:14pt; line-height:115%; margin:0pt 0pt 10pt; orphans:0; text-align:justify; widows:0"><span style="font-family:'Times New Roman'; font-size:14pt">&#xa0;</span></p>
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        <div style="width:100%;">
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<img src="https://static.igem.org/mediawiki/2020/6/6d/T--Xiamen_city--homepage04.jpg" style="width: 100%;" />
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                        <p style="font-size:48px; line-height:200%; margin:15.6pt 0pt; orphans:0; text-align:center; widows:0"><span
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                            style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:48px; font-weight:bold">Engineering</span></p>
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                        <p style="font-size:36px; line-height:200%; margin:15.6pt 0pt; orphans:0; text-align:justify; widows:0"><span
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                            style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:36px; font-weight:bold">Introduction </span></p>
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                        <p style="font-size:22px; line-height:200%; margin:15.6pt 0pt; orphans:0; text-align:justify; widows:0"><span
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                            style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Arsenic is widely distributed in the natural environment.
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                            Trace amounts of arsenic can be detected in soil, water, minerals, and plants. Among many environmental
 +
                            pollutants, arsenic ranks first on the blacklist of polluting toxic elements and is also a powerful carcinogen.
 +
                            In many countries, arsenic causes water, soil and crop pollution. We design an arsenic-induced response system
 +
                            in the cell or on the surface of the engineered probiotic bacteria to detect the content of arsenite in the
 +
                            environment, provide warnings to people, and reduce the health damage it causes to the human body. </span></p>
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                        <p style="font-size:36px; line-height:200%; margin:15.6pt 0pt; orphans:0; text-align:justify; widows:0"><span
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                            style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:36px; font-weight:bold">Design</span><span
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                            style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px"> </span></p>
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                        <p style="font-size:22px; line-height:200%; margin:15.6pt 0pt; orphans:0; text-align:justify; widows:0"><span
 +
                            style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Three proteins ArsR, ArsD, and ArsA belong to the operon
 +
                            family. The cysteine thiolates on their amino acid sequences can form a three-coordinate complex with arsenic
 +
                            (iii), which is self-sensing to arsenic. </span></p>
 +
                        <p style="font-size:22px; line-height:200%; margin:15.6pt 0pt; orphans:0; text-align:justify; widows:0"><span
 +
                            style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Under the induction of arsenite, ArsD gene is transcript
 +
                            and enxpressed in </span><span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">E.
 +
                            coli</span><span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px"> cells. At the same time, a reporter gene
 +
                            GFP is connected to the ArsD gene. Then, when the concentration of arsenite in the environment, the expression
 +
                            of ArsD-GFP can be detected by observing the intensity of the fluorescent signal.   </span></p>
 +
                        <p style="font-size:36px; line-height:200%; margin:15.6pt 0pt; orphans:0; text-align:justify; widows:0"><span
 +
                            style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:36px; font-weight:bold">Build </span></p>
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                        <p style="font-size:22px; line-height:200%; margin:15.6pt 0pt; orphans:0; text-align:justify; widows:0"><span
 +
                            style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Three recombinant plasmids pUC57-ArsD-amilGFP (Figure 1),
 +
                            pUC57-ArsR-amilGFP, and pUC57-ArsA-amilGFP were constructed in order to select the most sensitive sensor to
 +
                            arsenic. All the constructs are confirmed by DNA sequencing.</span></p>
 +
                        <p style="font-size:22px; line-height:200%; margin:15.6pt 0pt; orphans:0; text-align:center; widows:0"><img
 +
                            src="https://static.igem.org/mediawiki/2021/4/4e/T--Shanghai_United--Engineering01.jpg" width="500" alt=""
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                            style="-aw-left-pos:0pt; -aw-rel-hpos:column; -aw-rel-vpos:paragraph; -aw-top-pos:0pt; -aw-wrap-type:inline" />
 +
                        </p>
 +
                        <p style="font-size:16px; line-height:200%; margin:15.6pt 0pt; orphans:0; text-align:center; widows:0"><span
 +
                            style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:16px">Figure 1. Schematic map of plasmids.</span></p>
 +
                        <p style="font-size:36px; line-height:200%; margin:15.6pt 0pt; orphans:0; text-align:justify; widows:0"><span
 +
                            style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:36px; font-weight:bold">Test </span></p>
 +
                        <p style="font-size:22px; line-height:200%; margin:15.6pt 0pt; orphans:0; text-align:justify; widows:0"><span
 +
                            style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Fluorescence intensity generated by an ARSD/amilGFP
 +
                            transformed E. coli reacting for 1 hour in C</span><span
 +
                            style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:16px; vertical-align:sub">2</span><span
 +
                            style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">H</span><span
 +
                            style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:16px; vertical-align:sub">6</span><span
 +
                            style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">AsNaO</span><span
 +
                            style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:16px; vertical-align:sub">5</span><span
 +
                            style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px"> </span><span
 +
                            style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">solutions. As seen in the figure, fluorescence was
 +
                            detected for all C</span><span
 +
                            style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:16px; vertical-align:sub">2</span><span
 +
                            style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">H</span><span
 +
                            style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:16px; vertical-align:sub">6</span><span
 +
                            style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">AsNaO</span><span
 +
                            style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:16px; vertical-align:sub">5</span><span
 +
                            style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px"> concentrations that are above 0, which confirms that the
 +
                            designed plasmid worked as intended (Figure 2).</span></p>
 +
                        <p style="font-size:12pt; line-height:130%; margin:3pt 0pt; orphans:0; text-align:center; widows:0"><img
 +
                            src="https://static.igem.org/mediawiki/2021/7/74/T--Shanghai_United--Engineering02.jpg" width="500" alt=""
 +
                            style="-aw-left-pos:0pt; -aw-rel-hpos:column; -aw-rel-vpos:paragraph; -aw-top-pos:0pt; -aw-wrap-type:inline" />
 +
                        </p>
 +
                        <p style="font-size:16px; line-height:200%; margin:15.6pt 0pt; orphans:0; text-align:center; widows:0"><span
 +
                            style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:16px">Figure 2: Fluorescence Intensity of Transformed E. coli In
 +
                            Different Concentrations of C</span><span
 +
                            style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:16px; vertical-align:sub">2</span><span
 +
                            style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:16px">H</span><span
 +
                            style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:16px; vertical-align:sub">6</span><span
 +
                            style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:16px">AsNaO</span><span
 +
                            style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:16px; vertical-align:sub">5</span><span
 +
                            style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:16px"> Solutions</span></p>
 +
                        <p style="font-size:36px; line-height:200%; margin:15.6pt 0pt; orphans:0; text-align:justify; widows:0"><span
 +
                            style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:36px; font-weight:bold">Learn</span></p>
 +
                        <p style="font-size:22px; line-height:200%; margin:15.6pt 0pt; orphans:0; text-align:justify; widows:0"><span
 +
                            style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">C</span><span
 +
                            style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:16px; vertical-align:sub">2</span><span
 +
                            style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">H</span><span
 +
                            style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:16px; vertical-align:sub">6</span><span
 +
                            style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">AsNaO</span><span
 +
                            style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:16px; vertical-align:sub">5</span><span
 +
                            style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px"> was used for this function test. The observed
 +
                            fluorescence intensity suggested that the engineered bacteria are functional. However, the fluorescence was
 +
                            rather minor, so we speculate that </span><span
 +
                            style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">E. coli</span><span
 +
                            style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px"> responds poorly to organic arsenic compounds. Further
 +
                            experiments would be conducted to test for fluorescence intensities of E. coli in inorganic arsenic solutions.  
 +
                          </span></p>
 +
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 +
 
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 +
                        <span
 +
                                style="font-size: 48px;display: block;padding-bottom: 70px;">CONTACT INFO</span>
 +
                        <span
 +
                                style="text-align: center;line-height: 90px;letter-spacing: 2px;font-size: 36px;display: block;font-family: -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen, Ubuntu, Cantarell, 'Open Sans', 'Helvetica Neue', sans-serif;">
 +
                                Please scan the following QR codes to find more about us on
 +
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 +
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 +
                                Weibo、Bilibili、Wechat !
 +
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        ! function () {
 +
                var layer = layui.layer,
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                        form = layui.form;
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        layui.use('carousel', function () {
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                var carousel = layui.carousel;
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                //建造实例
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                carousel.render({
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<span style="font-family: Athelas; font-size: 12pt; font-weight: bold;">Contact Email: ni3222601872@gmail.com
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<span style="font-family: Athelas; font-size: 12pt; font-weight: bold">Wechat: Search "NapTuner" in WeChat</span>
+
                        , arrow: 'always' //始终显示箭头
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                        //,anim: 'updown' //切换动画方式
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</div>
+
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Revision as of 02:49, 12 October 2021

Document

Engineering

Introduction

Arsenic is widely distributed in the natural environment. Trace amounts of arsenic can be detected in soil, water, minerals, and plants. Among many environmental pollutants, arsenic ranks first on the blacklist of polluting toxic elements and is also a powerful carcinogen. In many countries, arsenic causes water, soil and crop pollution. We design an arsenic-induced response system in the cell or on the surface of the engineered probiotic bacteria to detect the content of arsenite in the environment, provide warnings to people, and reduce the health damage it causes to the human body.

Design

Three proteins ArsR, ArsD, and ArsA belong to the operon family. The cysteine thiolates on their amino acid sequences can form a three-coordinate complex with arsenic (iii), which is self-sensing to arsenic.

Under the induction of arsenite, ArsD gene is transcript and enxpressed in E. coli cells. At the same time, a reporter gene GFP is connected to the ArsD gene. Then, when the concentration of arsenite in the environment, the expression of ArsD-GFP can be detected by observing the intensity of the fluorescent signal.  

Build

Three recombinant plasmids pUC57-ArsD-amilGFP (Figure 1), pUC57-ArsR-amilGFP, and pUC57-ArsA-amilGFP were constructed in order to select the most sensitive sensor to arsenic. All the constructs are confirmed by DNA sequencing.

Figure 1. Schematic map of plasmids.

Test

Fluorescence intensity generated by an ARSD/amilGFP transformed E. coli reacting for 1 hour in C2H6AsNaO5 solutions. As seen in the figure, fluorescence was detected for all C2H6AsNaO5 concentrations that are above 0, which confirms that the designed plasmid worked as intended (Figure 2).

Figure 2: Fluorescence Intensity of Transformed E. coli In Different Concentrations of C2H6AsNaO5 Solutions

Learn

C2H6AsNaO5 was used for this function test. The observed fluorescence intensity suggested that the engineered bacteria are functional. However, the fluorescence was rather minor, so we speculate that E. coli responds poorly to organic arsenic compounds. Further experiments would be conducted to test for fluorescence intensities of E. coli in inorganic arsenic solutions.  

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