− | <!-- # TODO: #6 Fix table caption font--><!-- # TODO: #7 Fix citations links font size--><html lang="en"><head><meta charset="utf-8"/><meta content="width=device-width,initial-scale=1" name="viewport"/><title>Engineering Success | iGEM Concordia-Montreal</title><link href="https://2021.igem.org/Template:Concordia-Montreal/css/contentCSS?action=raw&ctype=text/css" rel="stylesheet"/></head><body><!-- # TODO: #6 Fix table caption font--><!-- # TODO: #7 Fix citations links font size--><nav class="navbar navbar-expand-xl fixed-top"><div class="container d-flex justify-content-between"><a class="navbar-brand d-lg-inline-block" href="https://2021.igem.org/Team:Concordia-Montreal"><span>iGEM Concordia</span></a><button aria-controls="navbarNav" aria-expanded="false" aria-label="Toggle navigation" class="navbar-toggler" data-target="#navbarNav" data-toggle="collapse" type="button"><span class="navbar-toggler-icon"></span></button><div class="collapse navbar-collapse" id="navbarNav"><ul 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class="sidebar col-lg-3"><div class="nav" id="contents"><h5>Contents</h5><ul></ul></div></div><div class="content col-lg-9"><article><h1>Introduction</h1><p>Last year, we built 2 microgravity simulators and developed one space bioreactor concept. Here we will discuss one of our experimental devices in-depth. The 3D clinostat is part of our engineering group�s undergraduate final year project. It was purposefully built for iGEM Concordia to run microgravity synthetic biology experiments.</p><p>Recently, we developed our experiment protocol. Our device is verifying our experiment methodologies and testing our hypothesis. We conducted a series of pilot experiments which demonstrated the effectiveness of our devices. For more information about the result of the pilot experiment, please see our genetics team page.</p><div class="image"><img alt="clinostat" src="https://static.igem.org/mediawiki/2021/4/4b/T--Concordia-Montreal--img--e_image10.gif"/><p>Figure 1: clinostat</p></div><p>We learned a lot from our last year�s project. We started the design phase by following this engineering flowchart:</p><div class="image"><img src="https://static.igem.org/mediawiki/2021/b/b7/T--Concordia-Montreal--img--e_image6.jpg"/><p>Figure 2: undefined</p></div><h1>Design</h1><p>We have implemented an iterative design approach during our design phase. The design process was lengthy in order to obtain the most optimized version of the 3D clinostat. Several aspects, such as manufacturing, budget, and feasibility of the design, were our main concerns. This design validation diagram summarises our process visually:</p><h2>First Design Iteration</h2><div class="image"><img alt="first iteration" src="https://static.igem.org/mediawiki/2021/6/69/T--Concordia-Montreal--img--e_image9.jpg"/><p>Figure 3: first iteration</p></div><p>Cons & Revisions:</p><ul><li>Bioreactor size & geometry could cause a large moment of inertia</li><li>Aluminum extrusions of the outer frame would cause poor wire management</li><li>Large aluminum base frames would be expensive, heavy and hard to manufacture/source.</li></ul><h2>Second Design Iteration</h2><div class="image"><img alt="second iteration" src="https://static.igem.org/mediawiki/2021/8/83/T--Concordia-Montreal--img--e_image2.jpg"/><p>Figure 4: second iteration</p></div><p>Cons & Revisions:</p><ul><li>Large aluminum base frames would be expensive, heavy and hard to manufacture/source.</li><li>Spherical bioreactor is not viable from a manufacturing standpoint as the two spherical halves of the bioreactor would be welded yielding a high stress concentration possibly leading to failure.</li></ul><h2>Final Design</h2><div class="image"><img alt="final design" src="https://static.igem.org/mediawiki/2021/5/59/T--Concordia-Montreal--img--e_image5.jpg"/><p>Figure 5: final design</p></div><p>Revisions & Improvements:</p><ul><li>Aluminum extrusions for the base frame to support the motors & payload.</li><li>Hollow sheet metal outer frame for easier wire management</li><li>Rectangular shaped bioreactor made from acrylic</li></ul><h1>Build</h1><p>With our final design in hand and feasibility verified, we moved on to the next stage. The finalized design components (including the electronics) are listed as:</p><div class="image"><img alt="" src="https://static.igem.org/mediawiki/2021/a/a0/T--Concordia-Montreal--img--e_image12.png"/><p>Figure 6:</p></div><p>During the building phase, we have revisioned our design in 2 parts to increase device stability and reduce cost. We implemented the laser cutting process, which is an easy-to-use process as it substitutes the more conventional cutting processes due to its economic and technical benefits. We have also revisioned our frame assembly manufacturing process. This minor revision reduces manufacturing difficulties. Our outer frame assembly was welded together and assembled to the whole construct.</p><div class="image"><img alt="left: revision drawing, right: outer frame assembly." src="https://static.igem.org/mediawiki/2021/3/31/T--Concordia-Montreal--img--e_image11.png"/><p>Figure 7: left: revision drawing, right: outer frame assembly.</p></div><p>The total engineering cost for our 3D clinostat was 800$ CAD, excluding operational costs.</p><h1>Test</h1><p>In order to validate our design, we had to test all aspects extensively, as shown in the testing diagram below.</p><div class="image"><img alt="" src="https://static.igem.org/mediawiki/2021/2/25/T--Concordia-Montreal--img--e_image4.png"/><p>Figure 8:</p></div><p>However, despite some minor tweaks to facilitate the assembly and quick modifications to the electronics, namely the stepper motors and drivers, to enhance the performance of the device, the 3D clinostat does achieve its function!</p><div class="image"><img alt="testing of each system function" src="https://static.igem.org/mediawiki/2021/c/cb/T--Concordia-Montreal--img--e_image1.png"/><p>Figure 9: testing of each system function</p></div><p>Finally, we collected data from the accelerometer placed in the center of rotation of the bioreactor to evaluate the different components of acceleration in the x, y, and z directions. Here are the resulting plots after 5 minutes of rotation.</p><div class="image"><img alt=" Acceleration data collected for all 3 axes after 5mns" src="https://static.igem.org/mediawiki/2021/3/3d/T--Concordia-Montreal--img--e_image14.png"/><p>Figure 10: Acceleration data collected for all 3 axes after 5mns</p></div><p>From the data collected from the ADXL 345 accelerometer after a sampling time of 360s, we can conclude that the rotation of the 3D Clinostat for an extended testing time of around 24h in a laboratory environment would most definitely showcase the damping or convergence of the sum of the acceleration in the x, y & z to 10^(-3) g, so as to achieve the simulation of microgravity due to its slow rotation as well as the constant dispersion of the gravity vector, as discussed given the relatively low values of acceleration in the y and z axes.</p><h1>Learn</h1><p>After we moved the clinostat to our iGEM lab, several issues were exposed. Those issues include excess vibration, installation error, false wiring connections, and overheating. Those issues are relatively minor issues and simple to solve.</p><div class="image"><img alt="issue with unsupported frame connectors" src="https://static.igem.org/mediawiki/2021/2/2a/T--Concordia-Montreal--img--e_image13.jpg"/><p>Figure 11: issue with unsupported frame connectors</p></div><p>However, the effect of vibration will be amplified on a cellular level which is much harder to solve.</p><h1>Improve</h1><p>We attached several support pieces which were 3D printed. Those pieces drastically improved our device stability.</p><div class="image"><img alt="" src="https://static.igem.org/mediawiki/2021/0/01/T--Concordia-Montreal--img--e_image3.png"/><p>Figure 12:</p></div><p>Friction is an inevitable factor when it comes to machines. Due to excess friction and vibration of the outer frame, we upgraded our stepper motor. The new motor provides more torque to sustain the unsupervised run.</p><div class="image"><img alt="" src="https://static.igem.org/mediawiki/2021/1/10/T--Concordia-Montreal--img--e_image8.png"/><p>Figure 13:</p></div><p>We have also learned due to the nature of the stepping motor, vibration from the motor was conducted to our inner bioreactor box. When vibration from the stepping motor reaches the resonance frequency of the inner box, it will create an oscillating motion. Thus, we have used</p></article></div></div></div></main><footer><img src="https://static.igem.org/mediawiki/2021/0/0b/T--Concordia-Montreal--img--Footer--sponsors.png" width="100%"/><br/><div class="container text-align-center"><div class="row"><div class="col-lg-6"><h2 class="fadjust">__________</h2><h2 class="fadjust">Discover SynBio Apps</h2><p>We are affiliated with Concordia's <a href="https://www.concordia.ca/research/casb.html">Centre for Applied Synthetic Biology (CASB)</a>, <a href="https://www.concordia.ca/sgs/programs/interdisciplinary/synthetic-biology-applications.html">SynBio Apps NSERC-CREATE</a>, and Canada's only academic <a href="https://www.concordia.ca/research/genome-foundry.html">Genome Foundry.</a></p><br/></div><div class="col-lg-1"></div><div class="col-lg-5"><h2 class="fadjust">__________</h2><h2 class="fadjust">Support Us</h2><p>To support the development and improvement of our project, we launched our own fundraising campaign available via Concordia's FundOne platform.<br/><a class="btn btn-primary btn-lg" href="https://www.concordia.ca/alumni-friends/giving-to-concordia/fundone/igem-concordia.html" role="button">Donate Now</a></p></div></div><br/><h2 class="mr-2 text-left"><a class="fadajust" href="https://www.facebook.com/iGEMConcordia"><i aria-hidden="true" class="fab fa-facebook-square"></i></a> <a class="fadajust" href="https://www.instagram.com/igem_concordia"><i aria-hidden="true" class="fab fa-instagram"></i></a> <a class="fadajust" href="https://twitter.com/iGEMConcordia"><i aria-hidden="true" class="fab fa-twitter-square"></i></a> <a class="fadajust" href="https://www.linkedin.com/company/concordia-igem"><i aria-hidden="true" class="fab fa-linkedin"></i></a></h2><p class="mr-2 text-right">© iGEM Concordia 2021</p></div></footer><script src="https://2021.igem.org/Template:Concordia-Montreal/content-bundleJS?action=raw&ctype=text/javascript"></script></body></html>
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class="sidebar col-lg-3"><div class="nav" id="contents"><h5>Contents</h5><ul></ul></div></div><div class="content col-lg-9"><article><h1>Introduction</h1><p>Last year, we built 2 microgravity simulators and developed one space bioreactor concept. Here we will discuss one of our experimental devices in-depth. The 3D clinostat is part of our engineering group�s undergraduate final year project. It was purposefully built for iGEM Concordia to run microgravity synthetic biology experiments.</p><p>Recently, we developed our experiment protocol. Our device is verifying our experiment methodologies and testing our hypothesis. We conducted a series of pilot experiments which demonstrated the effectiveness of our devices. For more information about the result of the pilot experiment, please see our genetics team page.</p><div class="image"><img alt="Clinostat" src="https://static.igem.org/mediawiki/2021/4/4b/T--Concordia-Montreal--img--e_image10.gif"/><p>Figure 1: Clinostat</p></div><p>We learned a lot from our last year�s project. We started the design phase by following this engineering flowchart:</p><div class="image"><img alt="Engineering Flowchart" src="https://static.igem.org/mediawiki/2021/b/b7/T--Concordia-Montreal--img--e_image6.jpg"/><p>Figure 2: Engineering Flowchart</p></div><h1>Design</h1><p>We have implemented an iterative design approach during our design phase. The design process was lengthy in order to obtain the most optimized version of the 3D clinostat. Several aspects, such as manufacturing, budget, and feasibility of the design, were our main concerns. This design validation diagram summarises our process visually:</p><h2>First Design Iteration</h2><div class="image"><img alt="First Iteration" src="https://static.igem.org/mediawiki/2021/6/69/T--Concordia-Montreal--img--e_image9.jpg"/><p>Figure 3: First Iteration</p></div><p>Cons & Revisions:</p><ul><li>Bioreactor size & geometry could cause a large moment of inertia</li><li>Aluminum extrusions of the outer frame would cause poor wire management</li><li>Large aluminum base frames would be expensive, heavy and hard to manufacture/source.</li></ul><h2>Second Design Iteration</h2><div class="image"><img alt="Second Iteration" src="https://static.igem.org/mediawiki/2021/8/83/T--Concordia-Montreal--img--e_image2.jpg"/><p>Figure 4: Second Iteration</p></div><p>Cons & Revisions:</p><ul><li>Large aluminum base frames would be expensive, heavy and hard to manufacture/source.</li><li>Spherical bioreactor is not viable from a manufacturing standpoint as the two spherical halves of the bioreactor would be welded yielding a high stress concentration possibly leading to failure.</li></ul><h2>Final Design</h2><div class="image"><img alt="Final Design" src="https://static.igem.org/mediawiki/2021/5/59/T--Concordia-Montreal--img--e_image5.jpg"/><p>Figure 5: Final Design</p></div><p>Revisions & Improvements:</p><ul><li>Aluminum extrusions for the base frame to support the motors & payload.</li><li>Hollow sheet metal outer frame for easier wire management</li><li>Rectangular shaped bioreactor made from acrylic</li></ul><h1>Build</h1><p>With our final design in hand and feasibility verified, we moved on to the next stage. The finalized design components (including the electronics) are listed as:</p><div class="image"><img alt="Design Components" src="https://static.igem.org/mediawiki/2021/a/a0/T--Concordia-Montreal--img--e_image12.png"/><p>Figure 6: Design Components</p></div><p>During the building phase, we have revisioned our design in 2 parts to increase device stability and reduce cost. We implemented the laser cutting process, which is an easy-to-use process as it substitutes the more conventional cutting processes due to its economic and technical benefits. We have also revisioned our frame assembly manufacturing process. This minor revision reduces manufacturing difficulties. Our outer frame assembly was welded together and assembled to the whole construct.</p><div class="image"><img alt="Left: Revision Drawing, Right: Outer Frame Assembly." src="https://static.igem.org/mediawiki/2021/3/31/T--Concordia-Montreal--img--e_image11.png"/><p>Figure 7: Left: Revision Drawing, Right: Outer Frame Assembly.</p></div><p>The total engineering cost for our 3D clinostat was 800$ CAD, excluding operational costs.</p><h1>Test</h1><p>In order to validate our design, we had to test all aspects extensively, as shown in the testing diagram below.</p><div class="image"><img alt="" src="https://static.igem.org/mediawiki/2021/2/25/T--Concordia-Montreal--img--e_image4.png"/><p>Figure 8:</p></div><p>However, despite some minor tweaks to facilitate the assembly and quick modifications to the electronics, namely the stepper motors and drivers, to enhance the performance of the device, the 3D clinostat does achieve its function!</p><div class="image"><img alt="System Function Testing" src="https://static.igem.org/mediawiki/2021/c/cb/T--Concordia-Montreal--img--e_image1.png"/><p>Figure 9: System Function Testing</p></div><p>Finally, we collected data from the accelerometer placed in the center of rotation of the bioreactor to evaluate the different components of acceleration in the x, y, and z directions. Here are the resulting plots after 5 minutes of rotation.</p><div class="image"><img alt=" Acceleration Data Collected For All 3 Axes After 5mns" src="https://static.igem.org/mediawiki/2021/3/3d/T--Concordia-Montreal--img--e_image14.png"/><p>Figure 10: Acceleration Data Collected For All 3 Axes After 5mns</p></div><p>From the data collected from the ADXL 345 accelerometer after a sampling time of 360s, we can conclude that the rotation of the 3D Clinostat for an extended testing time of around 24h in a laboratory environment would most definitely showcase the damping or convergence of the sum of the acceleration in the x, y & z to 10^(-3) g, so as to achieve the simulation of microgravity due to its slow rotation as well as the constant dispersion of the gravity vector, as discussed given the relatively low values of acceleration in the y and z axes.</p><h1>Learn</h1><p>After we moved the clinostat to our iGEM lab, several issues were exposed. Those issues include excess vibration, installation error, false wiring connections, and overheating. Those issues are relatively minor issues and simple to solve.</p><div class="image"><img alt="Issue With Unsupported Frame Connectors" src="https://static.igem.org/mediawiki/2021/2/2a/T--Concordia-Montreal--img--e_image13.jpg"/><p>Figure 11: Issue With Unsupported Frame Connectors</p></div><p>However, the effect of vibration will be amplified on a cellular level which is much harder to solve.</p><h1>Improve</h1><p>We attached several support pieces which were 3D printed. Those pieces drastically improved our device stability.</p><div class="image"><img alt="Improvements" src="https://static.igem.org/mediawiki/2021/0/01/T--Concordia-Montreal--img--e_image3.png"/><p>Figure 12: Improvements</p></div><p>Friction is an inevitable factor when it comes to machines. Due to excess friction and vibration of the outer frame, we upgraded our stepper motor. The new motor provides more torque to sustain the unsupervised run.</p><div class="image"><img alt="Motor" src="https://static.igem.org/mediawiki/2021/1/10/T--Concordia-Montreal--img--e_image8.png"/><p>Figure 13: Motor</p></div><p>We have also learned due to the nature of the stepping motor, vibration from the motor was conducted to our inner bioreactor box. When vibration from the stepping motor reaches the resonance frequency of the inner box, it will create an oscillating motion. Thus, we have used</p></article></div></div></div></main><footer><img src="https://static.igem.org/mediawiki/2021/0/0b/T--Concordia-Montreal--img--Footer--sponsors.png" width="100%"/><br/><div class="container text-align-center"><div class="row"><div class="col-lg-6"><h2 class="fadjust">__________</h2><h2 class="fadjust">Discover SynBio Apps</h2><p>We are affiliated with Concordia's <a href="https://www.concordia.ca/research/casb.html">Centre for Applied Synthetic Biology (CASB)</a>, <a href="https://www.concordia.ca/sgs/programs/interdisciplinary/synthetic-biology-applications.html">SynBio Apps NSERC-CREATE</a>, and Canada's only academic <a href="https://www.concordia.ca/research/genome-foundry.html">Genome Foundry.</a></p><br/></div><div class="col-lg-1"></div><div class="col-lg-5"><h2 class="fadjust">__________</h2><h2 class="fadjust">Support Us</h2><p>To support the development and improvement of our project, we launched our own fundraising campaign available via Concordia's FundOne platform.<br/><a class="btn btn-primary btn-lg" href="https://www.concordia.ca/alumni-friends/giving-to-concordia/fundone/igem-concordia.html" role="button">Donate Now</a></p></div></div><br/><h2 class="mr-2 text-left"><a class="fadajust" href="https://www.facebook.com/iGEMConcordia"><i aria-hidden="true" class="fab fa-facebook-square"></i></a> <a class="fadajust" href="https://www.instagram.com/igem_concordia"><i aria-hidden="true" class="fab fa-instagram"></i></a> <a class="fadajust" href="https://twitter.com/iGEMConcordia"><i aria-hidden="true" class="fab fa-twitter-square"></i></a> <a class="fadajust" href="https://www.linkedin.com/company/concordia-igem"><i aria-hidden="true" class="fab fa-linkedin"></i></a></h2><p class="mr-2 text-right">© iGEM Concordia 2021</p></div></footer><script src="https://2021.igem.org/Template:Concordia-Montreal/content-bundleJS?action=raw&ctype=text/javascript"></script></body></html> |