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<!--text--> | <!--text--> | ||
<p style="font-size:48pt; line-height:200%; margin:70pt; orphans:0; text-align:center; widows:0"><span | <p style="font-size:48pt; line-height:200%; margin:70pt; orphans:0; text-align:center; widows:0"><span | ||
− | style="color:#1f497d;font-family:' | + | style="color:#1f497d;font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:48pt; font-weight:bold">Proof of Concept</span></p> |
</hr> | </hr> | ||
<div style="width:40%;margin: 0 auto 100pt auto;"> | <div style="width:40%;margin: 0 auto 100pt auto;"> | ||
<div> | <div> | ||
− | <p style="font-size:36pt; line-height:200%; margin:0pt; orphans:0; text-align:justify; widows:0"><span | + | <p style="font-size:36pt; line-height:200%; margin:50px 0pt; orphans:0; text-align:justify; widows:0"><span |
− | style="font-family:' | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:36pt; font-weight:bold">Product </span><span |
− | style="font-family:' | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:36pt; font-weight:bold">D</span><span |
− | style="font-family:' | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:36pt; font-weight:bold">esign</span></p> |
<p style="font-size:10.5pt; line-height:200%; margin:0pt; orphans:0; text-align:center; widows:0"><img | <p style="font-size:10.5pt; line-height:200%; margin:0pt; orphans:0; text-align:center; widows:0"><img | ||
src="https://static.igem.org/mediawiki/2021/7/7f/T--Shanghai_United--Proof_of_Concept01.jpg" width="479" height="315" alt="" | src="https://static.igem.org/mediawiki/2021/7/7f/T--Shanghai_United--Proof_of_Concept01.jpg" width="479" height="315" alt="" | ||
style="-aw-left-pos:0pt; -aw-rel-hpos:column; -aw-rel-vpos:paragraph; -aw-top-pos:0pt; -aw-wrap-type:inline" /> | style="-aw-left-pos:0pt; -aw-rel-hpos:column; -aw-rel-vpos:paragraph; -aw-top-pos:0pt; -aw-wrap-type:inline" /> | ||
</p> | </p> | ||
− | <p style="font-size: | + | <p style="font-size:17px; line-height:200%; margin:50px 0pt; orphans:0; text-align:center; widows:0"><span |
− | style="font-family:' | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:17px; font-weight:bold">Figure 1. Design Drawing of the |
product - Arsenic Biosensor</span></p> | product - Arsenic Biosensor</span></p> | ||
<p | <p | ||
− | style="font-size: | + | style="font-size:22px; line-height:130%; margin:3pt 0pt; orphans:0; text-align:justify; text-indent:21pt; widows:0"> |
− | <span style="color:#333333; font-family:' | + | <span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">We plan to design an arsenic |
biosensor by genetically engineering </span><span | biosensor by genetically engineering </span><span | ||
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">E. |
− | coli</span><span style="color:#333333; font-family:' | + | coli</span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px"> to alarm people |
by giving the sign of green fluorescence when sensing the existence of the environmental arsenic. | by giving the sign of green fluorescence when sensing the existence of the environmental arsenic. | ||
</span></p> | </span></p> | ||
<p | <p | ||
− | style="font-size: | + | style="font-size:22px; line-height:130%; margin:3pt 0pt; orphans:0; text-align:justify; text-indent:21pt; widows:0"> |
− | <span style="color:#333333; font-family:' | + | <span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">As showing in the figure 1, our |
product will be used as a home kit for daily life and will be designed as possible as portable for our | product will be used as a home kit for daily life and will be designed as possible as portable for our | ||
− | custo</span><span style="color:#333333; font-family:' | + | custo</span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">mers to carry |
with. Besides, we will combine the tube with a reference card to determine the amount of the | with. Besides, we will combine the tube with a reference card to determine the amount of the | ||
environmental arsenic. </span></p> | environmental arsenic. </span></p> | ||
− | <p style="font-size:36pt; line-height:200%; margin:0pt; orphans:0; text-align:justify; widows:0"><span | + | <p style="font-size:36pt; line-height:200%; margin:50px 0pt; orphans:0; text-align:justify; widows:0"><span |
− | style="font-family:' | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:36pt; font-weight:bold">Engineered Bacteria</span></p> |
− | <p style="font-size: | + | <p style="font-size:22px; line-height:130%; text-align:center; margin:3pt 0pt; orphans:0; widows:0"><img |
src="https://static.igem.org/mediawiki/2021/e/ed/T--Shanghai_United--Proof_of_Concept02.jpg" width="605" height="370" alt="" | src="https://static.igem.org/mediawiki/2021/e/ed/T--Shanghai_United--Proof_of_Concept02.jpg" width="605" height="370" alt="" | ||
style="-aw-left-pos:0pt; -aw-rel-hpos:column; -aw-rel-vpos:paragraph; -aw-top-pos:0pt; -aw-wrap-type:inline" /> | style="-aw-left-pos:0pt; -aw-rel-hpos:column; -aw-rel-vpos:paragraph; -aw-top-pos:0pt; -aw-wrap-type:inline" /> | ||
</p> | </p> | ||
− | <p style="font-size: | + | <p style="font-size:17px; line-height:130%; margin:50px 0pt; orphans:0; text-align:center; widows:0"><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:17px; font-weight:bold">Figure |
</span><span | </span><span | ||
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:17px; font-weight:bold">2</span><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:17px; font-weight:bold">: The Self |
Defense Mechanism of </span><span | Defense Mechanism of </span><span | ||
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:17px; font-style:italic; font-weight:bold">E. |
− | coli</span><span style="color:#333333; font-family:' | + | coli</span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:17px; font-weight:bold"> |
</span><span | </span><span | ||
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:17px; font-weight:bold">i</span><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:17px; font-weight:bold">n</span><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:17px; font-weight:bold"> the</span><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:17px; font-weight:bold"> Presence of |
Arsenic</span></p> | Arsenic</span></p> | ||
<p | <p | ||
− | style="font-size: | + | style="font-size:22px; line-height:130%; margin:3pt 0pt; orphans:0; text-align:justify; text-indent:21pt; widows:0"> |
− | <span style="color:#333333; font-family:' | + | <span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">E.</span><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">coli</span><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic"> </span><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">will naturally react when arsenic |
is present in the environment with a series of defense metabolisms. According to Figure </span><span | is present in the environment with a series of defense metabolisms. According to Figure </span><span | ||
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">2</span><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">, when arsenic is present in the |
cytosol of </span><span | cytosol of </span><span | ||
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">E. |
− | coli</span><span style="color:#333333; font-family:' | + | coli</span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">, it will bind to |
− | </span><span style="color:#333333; font-family:' | + | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">ArsD</span><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px"> and </span><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">ArsR</span><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px"> proteins, which, when not bound to |
− | arsenic, inhibi</span><span style="color:#333333; font-family:' | + | arsenic, inhibi</span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">ts the |
expression of ARSA, ARSB, and ARSC genes, which are all located downstream of ARSD and ARSR genes. When | expression of ARSA, ARSB, and ARSC genes, which are all located downstream of ARSD and ARSR genes. When | ||
− | </span><span style="color:#333333; font-family:' | + | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">ArsD</span><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px"> and </span><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">ArsR</span><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px"> proteins bind to arsenic, it will |
no longer inhibit the expression of downstream genes. Additionally, </span><span | no longer inhibit the expression of downstream genes. Additionally, </span><span | ||
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">ArsA</span><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px"> proteins may also bind |
− | t</span><span style="color:#333333; font-family:' | + | t</span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">o arsenic, and the |
− | </span><span style="color:#333333; font-family:' | + | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">ArsA</span><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">-arsenic complex may also promote |
− | the exp</span><span style="color:#333333; font-family:' | + | the exp</span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">r</span><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">ession of downstream genes (not |
shown in figure). </span></p> | shown in figure). </span></p> | ||
− | <p style="font-size:36pt; line-height:200%; margin:0pt; orphans:0; text-align:justify; widows:0"><span | + | <p style="font-size:36pt; line-height:200%; margin:50px 0pt; orphans:0; text-align:justify; widows:0"><span |
− | style="font-family:' | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:36pt; font-weight:bold">Plasmid Construction</span></p> |
− | <p style="font-size: | + | <p style="font-size:22px; line-height:130%; margin:3pt 0pt; orphans:0; text-align:center; widows:0"><img |
src="https://static.igem.org/mediawiki/2021/7/7c/T--Shanghai_United--Proof_of_Concept03.jpg" width="605" height="570" alt="" | src="https://static.igem.org/mediawiki/2021/7/7c/T--Shanghai_United--Proof_of_Concept03.jpg" width="605" height="570" alt="" | ||
style="-aw-left-pos:0pt; -aw-rel-hpos:column; -aw-rel-vpos:paragraph; -aw-top-pos:0pt; -aw-wrap-type:inline" /> | style="-aw-left-pos:0pt; -aw-rel-hpos:column; -aw-rel-vpos:paragraph; -aw-top-pos:0pt; -aw-wrap-type:inline" /> | ||
</p> | </p> | ||
− | <p style="font-size: | + | <p style="font-size:17px; line-height:130%; margin:50px 0pt; orphans:0; text-align:center; widows:0"><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:17px; font-weight:bold">Figure |
</span><span | </span><span | ||
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:17px; font-weight:bold">3</span><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:17px; font-weight:bold">: Plasmid Map of |
ARS/</span><span | ARS/</span><span | ||
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:17px; font-weight:bold">amilGFP</span><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:17px; font-weight:bold"> Plasmids |
Transferred </span><span | Transferred </span><span | ||
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:17px; font-weight:bold">Into</span><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:17px; font-weight:bold"> Competent |
</span><span | </span><span | ||
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:17px; font-style:italic; font-weight:bold">E. |
coli</span></p> | coli</span></p> | ||
<p | <p | ||
− | style="font-size: | + | style="font-size:22px; line-height:130%; margin:3pt 0pt; orphans:0; text-align:justify; text-indent:21pt; widows:0"> |
− | <span style="color:#333333; font-family:' | + | <span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">T</span><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">he plasmid </span><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">would be</span><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px"> constructed in such a way that |
− | a</span><span style="color:#333333; font-family:' | + | a</span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">n</span><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px"> </span><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">amilGFP</span><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px"> sequence directly follows a single |
ARS sequence that is ARSA, ARSD, or ARSR. For instance, in Figure </span><span | ARS sequence that is ARSA, ARSD, or ARSR. For instance, in Figure </span><span | ||
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">3</span><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">, said </span><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">amilGFP</span><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px"> sequence follows a</span><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">n</span><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px"> ARSR sequence. When arsenic that |
− | </span><span style="color:#333333; font-family:' | + | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">is </span><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">present in the cytosol of |
− | </span><span style="color:#333333; font-family:' | + | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">E. |
− | coli</span><span style="color:#333333; font-family:' | + | coli</span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px"> binds |
− | </span><span style="color:#333333; font-family:' | + | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">to the Ars proteins, the |
− | inhibition </span><span style="color:#333333; font-family:' | + | inhibition </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">of |
− | </span><span style="color:#333333; font-family:' | + | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">the expression of |
− | </span><span style="color:#333333; font-family:' | + | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">amilGFP</span><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px"> is ceased, allowing </span><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">amil</span><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">GFP</span><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px"> to be expressed. Hence, green |
− | </span><span style="color:#333333; font-family:' | + | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">fluorescence</span><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px"> in the </span><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">E. |
− | coli</span><span style="color:#333333; font-family:' | + | coli</span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px"> would indicate |
the </span><span | the </span><span | ||
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">prescence</span><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px"> of arsenic. </span></p> |
− | <p style="font-size: | + | <p style="font-size:22px; line-height:130%; margin:3pt 0pt; orphans:0; text-indent:21pt; widows:0"><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px"> </span></p> |
<p style="font-size:48pt; line-height:200%; margin:70pt 0; orphans:0; text-align:center; widows:0"><span | <p style="font-size:48pt; line-height:200%; margin:70pt 0; orphans:0; text-align:center; widows:0"><span | ||
− | style="color:#1f497d; font-family:' | + | style="color:#1f497d; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:48pt; font-weight:bold">Proof by |
Experiments</span></p> | Experiments</span></p> | ||
− | <p style="font-size:36pt; line-height:200%; margin:0pt; orphans:0; text-align:justify; widows:0"><span | + | <p style="font-size:36pt; line-height:200%; margin:50px 0pt; orphans:0; text-align:justify; widows:0"><span |
− | style="font-family:' | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:36pt; font-weight:bold">Validation of the Plasmid |
− | </span><span style="font-family:' | + | </span><span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:36pt; text-align:center; font-weight:bold">Construction</span></p> |
− | <p style="font-size: | + | <p style="font-size:22px; line-height:130%; margin:3pt 0pt; orphans:0; text-align:center; widows:0"><img |
src="https://static.igem.org/mediawiki/2021/3/3d/T--Shanghai_United--Proof_of_Concept04.jpg" width="605" height="458" alt="" | src="https://static.igem.org/mediawiki/2021/3/3d/T--Shanghai_United--Proof_of_Concept04.jpg" width="605" height="458" alt="" | ||
style="-aw-left-pos:0pt; -aw-rel-hpos:column; -aw-rel-vpos:paragraph; -aw-top-pos:0pt; -aw-wrap-type:inline" /> | style="-aw-left-pos:0pt; -aw-rel-hpos:column; -aw-rel-vpos:paragraph; -aw-top-pos:0pt; -aw-wrap-type:inline" /> | ||
</p> | </p> | ||
− | <p style="font-size: | + | <p style="font-size:17px; line-height:200%; margin:50px 0pt; orphans:0; text-align:center; widows:0"><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:17px; font-weight:bold">Figure |
</span><span | </span><span | ||
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:17px; font-weight:bold">4</span><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:17px; font-weight:bold">: Gel |
Electrophoresis Results 1 of Colony PCR of Second Experimental Attempt</span></p> | Electrophoresis Results 1 of Colony PCR of Second Experimental Attempt</span></p> | ||
− | <p style="font-size: | + | <p style="font-size:22px; line-height:130%; margin:3pt 0pt; orphans:0; text-align:center; widows:0"><img |
src="https://static.igem.org/mediawiki/2021/5/58/T--Shanghai_United--Proof_of_Concept05.jpg" width="605" height="458" alt="" | src="https://static.igem.org/mediawiki/2021/5/58/T--Shanghai_United--Proof_of_Concept05.jpg" width="605" height="458" alt="" | ||
style="-aw-left-pos:0pt; -aw-rel-hpos:column; -aw-rel-vpos:paragraph; -aw-top-pos:0pt; -aw-wrap-type:inline" /> | style="-aw-left-pos:0pt; -aw-rel-hpos:column; -aw-rel-vpos:paragraph; -aw-top-pos:0pt; -aw-wrap-type:inline" /> | ||
</p> | </p> | ||
− | <p style="font-size: | + | <p style="font-size:17px; line-height:200%; margin:50px 0pt; orphans:0; text-align:center; widows:0"><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:17px; font-weight:bold">Figure |
</span><span | </span><span | ||
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:17px; font-weight:bold">5.</span><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:17px; font-weight:bold"> Gel |
Electrophoresis Results 2 of Colony PCR of Second Experimental Attempt</span></p> | Electrophoresis Results 2 of Colony PCR of Second Experimental Attempt</span></p> | ||
<p | <p | ||
− | style="font-size: | + | style="font-size:22px; line-height:130%; margin:3pt 0pt; orphans:0; text-align:justify; text-indent:21pt; widows:0"> |
− | <span style="color:#333333; font-family:' | + | <span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Figures </span><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">4</span><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px"> and </span><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">5</span><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px"> show the result for colony PCR |
identification on the </span><span | identification on the </span><span | ||
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">E.coli</span><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px"> with </span><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">ARS/</span><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">amilGFP</span><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px"> inserted that were cultivated |
previously. </span><span | previously. </span><span | ||
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">A</span><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">ll samples are determined to have |
bands of corresponding molecular sizes, which shows that all selected colonies yielded positive results | bands of corresponding molecular sizes, which shows that all selected colonies yielded positive results | ||
of </span><span | of </span><span | ||
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">ARS </span><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">and </span><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">amilGFP</span><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic"> </span><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">genes. </span></p> |
<p | <p | ||
− | style="font-size: | + | style="font-size:22px; line-height:130%; margin:3pt 0pt; orphans:0; text-align:justify; text-indent:21pt; widows:0"> |
− | <span style="color:#333333; font-family:' | + | <span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-weight:normal">In addition, |
we also sent these </span><span | we also sent these </span><span | ||
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-weight:normal">plamids</span><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-weight:normal"> to the |
sequencing company and the feedback was back</span><span | sequencing company and the feedback was back</span><span | ||
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-weight:normal"> up our |
expectation that all the plasmids were constructed correctly.</span></p> | expectation that all the plasmids were constructed correctly.</span></p> | ||
− | <p style="font-size:36pt; line-height:200%; margin:0pt; orphans:0; text-align:justify; widows:0"><span | + | <p style="font-size:36pt; line-height:200%; margin:50px 0pt; orphans:0; text-align:justify; widows:0"><span |
− | style="font-family:' | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:36pt; font-weight:bold">Validation of the Engineered |
Bacteria</span></p> | Bacteria</span></p> | ||
− | <p style="font-size: | + | <p style="font-size:17px; line-height:200%; margin:50px 0pt; orphans:0; text-align:justify; widows:0"><span |
− | style="color:#1f497d; font-family:' | + | style="color:#1f497d; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:17px; font-weight:bold"> </span></p> |
− | <p style="font-size: | + | <p style="font-size:22px; line-height:130%; text-align:center; margin:3pt 0pt; orphans:0; widows:0"><img |
src="https://static.igem.org/mediawiki/2021/8/8e/T--Shanghai_United--Proof_of_Concept06.jpg" width="508" height="355" alt="" | src="https://static.igem.org/mediawiki/2021/8/8e/T--Shanghai_United--Proof_of_Concept06.jpg" width="508" height="355" alt="" | ||
style="-aw-left-pos:0pt; -aw-rel-hpos:column; -aw-rel-vpos:paragraph; -aw-top-pos:0pt; -aw-wrap-type:inline" /> | style="-aw-left-pos:0pt; -aw-rel-hpos:column; -aw-rel-vpos:paragraph; -aw-top-pos:0pt; -aw-wrap-type:inline" /> | ||
</p> | </p> | ||
− | <p style="font-size: | + | <p style="font-size:17px; line-height:130%; margin:50px 0pt; orphans:0; text-align:center; widows:0"><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:17px; font-weight:bold">Figure |
</span><span | </span><span | ||
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:17px; font-weight:bold">6.</span><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:17px; font-weight:bold"> Fluorescence |
Intensity of Transformed </span><span | Intensity of Transformed </span><span | ||
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:17px; font-style:italic; font-weight:bold">E. |
− | coli</span><span style="color:#333333; font-family:' | + | coli</span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:17px; font-weight:bold"> |
In Different Concentrations of C</span><span | In Different Concentrations of C</span><span | ||
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:6pt; font-weight:bold; vertical-align:sub">2</span><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:17px; font-weight:bold">H</span><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:6pt; font-weight:bold; vertical-align:sub">12</span><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:17px; font-weight:bold">AsNaO</span><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:6pt; font-weight:bold; vertical-align:sub">5</span><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:17px; font-weight:bold"> Solutions</span> |
</p> | </p> | ||
<p | <p | ||
− | style="font-size: | + | style="font-size:22px; line-height:130%; margin:3pt 0pt; orphans:0; text-align:justify; text-indent:21pt; widows:0"> |
− | <span style="color:#333333; font-family:' | + | <span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Figure </span><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">6</span><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px"> displays the fluorescence |
intensity generated by a</span><span | intensity generated by a</span><span | ||
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">n</span><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px"> ARSD/</span><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">amilGFP</span><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px"> transformed E. coli reacting for 1 |
hour in C</span><span | hour in C</span><span | ||
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:8pt; vertical-align:sub">2</span><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">H</span><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:8pt; vertical-align:sub">12</span><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">AsNaO</span><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:8pt; vertical-align:sub">5</span><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px"> solutions. As </span><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">showing above, the </span><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">fluorescence</span><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px"> was detected for all C</span><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:8pt; vertical-align:sub">2</span><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">H</span><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:8pt; vertical-align:sub">12</span><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">AsNaO</span><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:8pt; vertical-align:sub">5</span><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px"> concentrations that are above 0, |
which confirms that the </span><span | which confirms that the </span><span | ||
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">e</span><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">ngineered</span><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic"> </span><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">E. |
− | coli</span><span style="color:#333333; font-family:' | + | coli</span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px"> </span><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">worked as intended. </span></p> |
<p | <p | ||
− | style="font-size: | + | style="font-size:22px; line-height:130%; margin:3pt 0pt; orphans:0; text-align:justify; text-indent:21pt; widows:0"> |
− | <span style="color:#333333; font-family:' | + | <span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">We will shortly carry on several |
function tests in some inorganic arsenic solutions and the water sample to solid our theory.</span></p> | function tests in some inorganic arsenic solutions and the water sample to solid our theory.</span></p> | ||
<p | <p | ||
− | style="font-size: | + | style="font-size:22px; line-height:130%; margin:3pt 0pt; orphans:0; text-align:justify; text-indent:21pt; widows:0"> |
− | <span style="color:#333333; font-family:' | + | <span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">In order to further confirm the |
− | </span><span style="color:#333333; font-family:' | + | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">quantitative relation |
between the </span><span | between the </span><span | ||
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">fluorescence intensity </span><span |
− | style="color:#333333; font-family:' | + | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">and the amounts of the arsenic, |
more function tests data is required. Once the model is built, it will be another milestone for the | more function tests data is required. Once the model is built, it will be another milestone for the | ||
development to implement our product - the arsenic biosensor.</span></p> | development to implement our product - the arsenic biosensor.</span></p> | ||
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<div style="background-color: #F9F9F9;"> | <div style="background-color: #F9F9F9;"> | ||
<div style="width: 80%;height: 1080px;margin: 0 auto;padding: 10% 0;"> | <div style="width: 80%;height: 1080px;margin: 0 auto;padding: 10% 0;"> | ||
− | <span style="font-size: 48px;display: block;padding-bottom: | + | <span |
+ | style="font-size: 48px;display: block;padding-bottom: 70px;">CONTACT INFO</span> | ||
<span | <span | ||
style="text-align: center;line-height: 90px;letter-spacing: 2px;font-size: 36px;display: block;font-family: -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen, Ubuntu, Cantarell, 'Open Sans', 'Helvetica Neue', sans-serif;"> | style="text-align: center;line-height: 90px;letter-spacing: 2px;font-size: 36px;display: block;font-family: -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen, Ubuntu, Cantarell, 'Open Sans', 'Helvetica Neue', sans-serif;"> | ||
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Weibo、Bilibili、Wechat ! | Weibo、Bilibili、Wechat ! | ||
</span> | </span> | ||
− | <img style="width:810px;height: 345px;margin: 100px auto;display: block;" | + | <img style="width:810px;height: 345px;margin: 100px auto;display: block;" src="https://static.igem.org/mediawiki/2021/1/1d/T--Shanghai_United--homepage06.jpg"> |
− | + | ||
</div> | </div> | ||
</div> | </div> |
Revision as of 03:21, 6 October 2021
Proof of Concept
Product Design
Figure 1. Design Drawing of the product - Arsenic Biosensor
We plan to design an arsenic biosensor by genetically engineering E. coli to alarm people by giving the sign of green fluorescence when sensing the existence of the environmental arsenic.
As showing in the figure 1, our product will be used as a home kit for daily life and will be designed as possible as portable for our customers to carry with. Besides, we will combine the tube with a reference card to determine the amount of the environmental arsenic.
Engineered Bacteria
Figure 2: The Self Defense Mechanism of E. coli in the Presence of Arsenic
E.coli will naturally react when arsenic is present in the environment with a series of defense metabolisms. According to Figure 2, when arsenic is present in the cytosol of E. coli, it will bind to ArsD and ArsR proteins, which, when not bound to arsenic, inhibits the expression of ARSA, ARSB, and ARSC genes, which are all located downstream of ARSD and ARSR genes. When ArsD and ArsR proteins bind to arsenic, it will no longer inhibit the expression of downstream genes. Additionally, ArsA proteins may also bind to arsenic, and the ArsA-arsenic complex may also promote the expression of downstream genes (not shown in figure).
Plasmid Construction
Figure 3: Plasmid Map of ARS/amilGFP Plasmids Transferred Into Competent E. coli
The plasmid would be constructed in such a way that an amilGFP sequence directly follows a single ARS sequence that is ARSA, ARSD, or ARSR. For instance, in Figure 3, said amilGFP sequence follows an ARSR sequence. When arsenic that is present in the cytosol of E. coli binds to the Ars proteins, the inhibition of the expression of amilGFP is ceased, allowing amilGFP to be expressed. Hence, green fluorescence in the E. coli would indicate the prescence of arsenic.
Proof by Experiments
Validation of the Plasmid Construction
Figure 4: Gel Electrophoresis Results 1 of Colony PCR of Second Experimental Attempt
Figure 5. Gel Electrophoresis Results 2 of Colony PCR of Second Experimental Attempt
Figures 4 and 5 show the result for colony PCR identification on the E.coli with ARS/amilGFP inserted that were cultivated previously. All samples are determined to have bands of corresponding molecular sizes, which shows that all selected colonies yielded positive results of ARS and amilGFP genes.
In addition, we also sent these plamids to the sequencing company and the feedback was back up our expectation that all the plasmids were constructed correctly.
Validation of the Engineered Bacteria
Figure 6. Fluorescence Intensity of Transformed E. coli In Different Concentrations of C2H12AsNaO5 Solutions
Figure 6 displays the fluorescence intensity generated by an ARSD/amilGFP transformed E. coli reacting for 1 hour in C2H12AsNaO5 solutions. As showing above, the fluorescence was detected for all C2H12AsNaO5 concentrations that are above 0, which confirms that the engineered E. coli worked as intended.
We will shortly carry on several function tests in some inorganic arsenic solutions and the water sample to solid our theory.
In order to further confirm the quantitative relation between the fluorescence intensity and the amounts of the arsenic, more function tests data is required. Once the model is built, it will be another milestone for the development to implement our product - the arsenic biosensor.