Genetics

Brian Baxter and Gabriel Aguiar-Tawil acted as co-leads for the Genetics subteam.

Gabriel Aguiar-Tawil worked on reporter construction, selection, stress experiments, (L)-limonene synthase construction and transformation and microgravity stress experiment.

Brian Baxter worked on reporter construction, selection, stress experiments, (L)-limonene synthase construction and transformation.

Amirhossein Rajabi Vajargah worked on stress experiments, (L)-limonene synthase construction and transformation.

Natasha Letourneau worked on reporter construction, selection, stress experiments, (L)-limonene synthase construction and transformation, graphs and data analysis, and microgravity stress experiment.

Paula Gomez worked on reporter construction, selection, stress experiments, (L)-limonene synthase construction and transformation.

Evelyn Huaman worked on proof of concept research, (L)-limonene synthase construct and primer design.

Hoang Nhi Nguyen worked on reporter construction.

Samman Zaman worked on proof of concept research.

Yunxuan (Paul) Bu worked on stress experiments, (L)-limonene synthase construction and transformation, microgravity stress experiment, and hardware maintenance.

Lancia Lefebvre worked on reporter construction, selection, stress experiments. Research into the requirements of yeast for growth in the bioreactor design.

Hardware

Miriam Hebbassi acted as lead for the Hardware subteam.

Yunxuan (Paul) Bu Current hardware team lead.

Miriam Hebbassi worked on the 3D clinostat construction and modeling, bioreactor project lead, bioreactor design and concept development, and supervised research and development.

Sean le Noble worked on bioreactor design and modeling.

Jenol Arulanatham worked on bioreactor design and modeling, and 3D clinostat construction and modeling.

Tiago Strobino worked on HARV maintenance and improvement, bioreactor control system.

Tim Freiman worked on bioreactor control system design.

Yunxuan (Paul) Bu worked on 3D clinostat troubleshooting and maintenance, microgravity experiment setup, bioreactor concept development, team collaboration and communication.

Software

Benjamin Clark acted as lead for the Software subteam.

Benjamin Clark leads shiny app development and data curation for AstroBio.

Marie-May Blier curated data for AstroBio.

Human Practices

Grecia O’Brien and Han (Stella) Vu acted as lead for the HP subteam.

Grecia O’Brien adapted the GE3LS framework to the Astroyeast 2021 project.

Han (Stella) Vu worked on the ethics and environmental portion of the GE3LS framework.

Carly Videira presented, organized and created the PowerPoint for diversity and inclusion workshop with uLaval; worked on planning and organizing the scientific presentation workshop and created the cheat sheet for scientific presentations.

Megan Lai Tung worked on the ethics and environmental portion of the GE3LS framework; organized and worked on the diversity and inclusion workshop with uLaval. Collaborated on the Rosalind chronicles project with uLaval, Patras and Thessaloniki. Created the cheat sheet for the 3 minute-thesis presentations. Helped conceive the I Pledge Campaign form along with Grecia.

Gabriel Aguiar-Tawil worked on Integrated HP, genetics portion of GE3LS framework.

Evelyn Huaman, Gabriel Aguiar-Tawil, Grecia OBrien, Lancia Lefevre worked on co-creating a workshop for the iGEM TAU (Ireland) x iGEM Concordia (Montreal) x iGEM BOKU-Vienna.

Carly Videira, Megan Lai Tung, and Grecia O’Brien worked on co-creating our Diversity & Inclusion workshop for the iGEM Concordia x iGEM ULaval collaboration x iGEM Patras x iGEM Thessaloniki.

Megan Lai Tung worked on the Rosalind Chronicles project in collaboration with iGEM ULaval, Patras, Thessaloniki.

Megan Lai Tung helped coordinate the iGEM Patras podcast participation.

Education & Science Communication

Grecia O’Brien, Evelyn Huaman, Megan Lai Tung and Han (Stella) Vu worked on developing content for our upcoming Synbio Talks podcast.

Entrepreneurship

Evelyn Huaman, Grecia O’Brien, Paula Andrea Gomez, Han (Stella) Vu participated in the Quebec Scientific Entrepreneurship program.

Sustainable Development

Grecia O’Brien with input from the other subteam leads helped identify which SGDs would be applicable to the assessment of Astroyeast 2021.

Roy Singer-Shay worked on a short video about UN SDGs the project is working on in collaboration with iGEM Patras (Greece).

Design & Social Media

Man Zou and NJ acted as lead for the Design & Social Media subteam.

Man Zou worked on weekly “Let Synbio Wow You” to promote scientific communication. Helped on organizing the Synbio Scavenger Hunt to promote scientific communication. Worked on the iGEM promotional video. Worked on the hardware bioreactor animation video. Worked on other graphic designs for social media, fundraising, and wiki. Participated in collaborations with other iGEM teams (Greeting Video_Nantes; Postcard Project Düsseldorf;).

Nallely J. Urbina (NJ) worked on weekly “Did You Know” to promote scientific communication. Worked on organizing the Synbio Scavenger Hunt to promote scientific communication. Worked on the iGEM promotional video. Helped with the hardware bioreactor animation video. Worked on other graphic designs for social media, fundraising, and wiki. Participated in collaborations with other iGEM teams (Lab Coat Challenge Sorbonne, Monument Challenge Paris-Saclay;).

Neha Mohammad worked on biweekly “Scientific Blurbs” and “Biweekly Interactive Quizzes” to promote scientific communication. Helped with other graphic designs for social media and fundraising.

Evelyn Namauh worked on weekly “Word of the Day” to promote scientific communication. Helped in organizing the Synbio Scavenger Hunt to promote scientific communication. Worked on the iGEM promotional video. Collaborated with McMaster University for Synbio Mashup Song Challenge. Participated along with Paula in a Spanish Radio Talk Show called Entre Generationes to talk about the project. Voice Narration and acting for iGEM promotional video in English, French and Spanish. Worked on Wiki Subteam Notebooks.

Yeekyung June Kim worked on biweekly “Synbio Leaders” sci-comms to promote scientific communication. Worked on other graphic designs for social media. Helped coordinate collaborations with other iGEM teams (Weekly Mood_Tirupati; Postcard Project Düsseldorf; interviews about our research Guelph).

Mohammadamin Nikpayam worked on the iGEM promotional video.

Paula Gomez participated with Evelyn Namauh in a Spanish Radio Talk Show called Entre Generationes to talk about the project. Helped with the iGEM promotional video.

Gabriel Aguiar-Tawil Helped with the iGEM promotional video.

Fundraising

Amirhossein Rajabi Vajargah acted as the lead for Fundraising subteam.

Amirhossein Rajabi Vajargah worked on writing content and setting up the FundOne fundraising platform. Oversaw fundraising activities. Helped in writing grant applications.

Nallely J. Urbina (NJ) worked on coordinating Scavenger hunt fundraising event.

Man Zou helped in writing content and setting up the FundOne fundraising platform. Helped in writing grant applications.

Gabriel Aguiar-Tawil helped in writing grant applications.

Lancia Lefebvre helped in writing grant applications and editing FundOne content.

Grecia O’Brien and Evelyn Namauh helped in fundraising activities.

Paula Gomez secured funding from external sources.

Wiki Content

Grecia O’Brien, Man Zou and Benjamin Clark worked on the wiki design and coding.

Genetics:

Introduction - Lancia

Transformation - Paula and Brian

Colony screening, strain selection and troubleshooting - Natasha

Evolution - Amir

Proof of concept molecule - Evelyn

Reporter design - Gabe

Moving forward - Samman

Hardware:

Paul: Abstract, Engineering success

Miriam: Abstract, Engineering success, 3D clinostat, modeling, AstroYeast Microfarm

Jenol: 3D clinostat, AstroYeast Microfarm

Tiago: HARV, AstroYeast Microfarm control

General:

Integrated human practices: Gabe, Carly and Megan

Proposed implementation: Gabe, Miriam, Lancia, Paul, NJ, Evelyn, Jenol, Sean

Project description: Gabe, Miriam, Lancia, Paul, NJ, Evelyn, Jenol, Sean

Mentors and advisors

Mohamed Nasr - Genetics

Steven Li - Hardware

Felipe Perez - Hardware

Jenny Ann Gagnon - Genetics, design and social media

Chiara Leal Alves - Hardware, fundraising, design and social media

Tina Papazotos - Human practices, fundraising

Michelle Vandeloo - Human practices

Maher Hassanain - Software

Acknowledgments

Orly Weinberg - Project Supervisor and primary PI

Dr. Nawwaf Kharma and Dr. Aashiq Kachroo - PIs

Dr. Vincent Martin and Dr. Bjorn Bean - Cas9 Plasmid

Dr. Brandiff Caron - Ethics Consultant

Dr. Roxanne Fournier - Space Nutrition Consultant

Dr. Ibrahim Babiker - 3D Clinostat Modeling Consultant

Dr. Marcel Egli & Dr. Tim Granata - Bioreactor Consultants

Center for Applied Synthetic Biology - Flow Cytometer (operated by Mohamed Nasr)

HARV team: Michael Carioti, Emilio Gonzalez, Jalen Charrette-Wells, Justin Campanelli, Michele Cinquino, Tong Yu

3D clinostat team: Cedric Li Luen Ching, David Castro, Jenol Arulanantham, Kevin Chow, Miriam Hebbassi, Sorelle Wuilé Sipeyou