(Prototype team page) |
|||
(8 intermediate revisions by the same user not shown) | |||
Line 1: | Line 1: | ||
− | + | <html lang="en"> | |
− | + | ||
− | <html> | + | |
− | < | + | <head> |
+ | <meta charset="UTF-8"> | ||
+ | <meta http-equiv="X-UA-Compatible" content="IE=edge"> | ||
+ | <meta name="viewport" content="width=device-width, initial-scale=1.0"> | ||
+ | <title>Document</title> | ||
+ | <link rel="stylesheet" | ||
+ | href="https://2021.igem.org/Team:Shanghai_United/Template:CSS?action=raw&ctype=text/css"> | ||
+ | <link rel="stylesheet" href="//unpkg.com/layui@2.6.8/dist/css/layui.css"> | ||
− | < | + | <script src="https://stackpath.bootstrapcdn.com/bootstrap/3.4.1/js/bootstrap.min.js" |
− | < | + | integrity="sha384-aJ21OjlMXNL5UyIl/XNwTMqvzeRMZH2w8c5cRVpzpU8Y5bApTppSuUkhZXN0VxHd" |
+ | crossorigin="anonymous"></script> | ||
+ | <script src="https://libs.baidu.com/jquery/1.4.2/jquery.min.js"></script> | ||
− | |||
− | |||
− | |||
− | </ | + | </head> |
+ | <style type="text/css"> | ||
+ | .reveal-modal-bg { | ||
+ | position: fixed; | ||
+ | height: 100%; | ||
+ | width: 100%; | ||
+ | z-index: 100; | ||
+ | display: none; | ||
+ | top: 0; | ||
+ | left: 0; | ||
+ | background: rgba(00, 00, 00, 0.8) | ||
+ | } | ||
+ | .reveal-modal { | ||
+ | visibility: hidden; | ||
+ | top: 150px; | ||
+ | position: absolute; | ||
+ | z-index: 101; | ||
+ | padding: 30px 40px 34px; | ||
+ | -moz-border-radius: 5px; | ||
+ | -webkit-border-radius: 5px; | ||
+ | border-radius: 5px; | ||
+ | -moz-box-shadow: 0 0 10px rgba(0, 0, 0, .4); | ||
+ | -webkit-box-shadow: 0 0 10px rgba(0, 0, 0, .4); | ||
+ | -box-shadow: 0 0 10px rgba(0, 0, 0, .4); | ||
+ | background-color: #FFF; | ||
+ | } | ||
+ | .reveal-modal.small { | ||
+ | width: 200px; | ||
+ | margin-left: -140px; | ||
+ | } | ||
− | + | .reveal-modal.medium { | |
− | + | width: 400px; | |
− | + | margin-left: -240px; | |
− | + | } | |
− | + | ||
− | + | ||
− | </div> | + | .reveal-modal.large { |
+ | width: 600px; | ||
+ | margin-left: -340px; | ||
+ | } | ||
+ | |||
+ | .reveal-modal.xlarge { | ||
+ | width: 800px; | ||
+ | margin-left: -440px; | ||
+ | } | ||
+ | |||
+ | .reveal-modal .close-reveal-modal { | ||
+ | font-size: 22px; | ||
+ | line-height: 0.5; | ||
+ | position: absolute; | ||
+ | top: 8px; | ||
+ | right: 11px; | ||
+ | color: #333; | ||
+ | text-shadow: 0 -1px 1px rbga(0, 0, 0, .6); | ||
+ | font-weight: bold; | ||
+ | cursor: pointer; | ||
+ | } | ||
+ | #sideMenu, | ||
+ | #top_title, | ||
+ | .patrollink, | ||
+ | #firstHeading, | ||
+ | #home_logo, | ||
+ | #sideMenu { | ||
+ | display: none; | ||
+ | } | ||
+ | |||
+ | #content { | ||
+ | padding: 0px; | ||
+ | width: 100%; | ||
+ | margin-top: -7px; | ||
+ | margin-left: 0px; | ||
+ | border: none; | ||
+ | } | ||
+ | |||
+ | body, | ||
+ | html { | ||
+ | background-color: white; | ||
+ | width: 100%; | ||
+ | height: 100%; | ||
+ | } | ||
+ | |||
+ | #bodyContent h1, | ||
+ | #bodyContent h2, | ||
+ | #bodyContent h3, | ||
+ | #bodyContent h4, | ||
+ | #bodyContent h5 { | ||
+ | margin-bottom: 0px; | ||
+ | } | ||
+ | |||
+ | #bodyContent a[href ^="https://"], | ||
+ | .link-https { | ||
+ | padding-right: 0px; | ||
+ | } | ||
+ | </style> | ||
+ | <style> | ||
+ | html { | ||
+ | width: 100%; | ||
+ | height: 100%; | ||
+ | } | ||
+ | |||
+ | body { | ||
+ | min-width: 1920px; | ||
+ | overflow: scroll; | ||
+ | margin: 0 auto; | ||
+ | } | ||
+ | |||
+ | .layui-nav .layui-nav-item a { | ||
+ | color: black; | ||
+ | font-size: 17px; | ||
+ | } | ||
+ | |||
+ | .layui-nav .layui-nav-item a:hover { | ||
+ | color: black; | ||
+ | text-decoration: none; | ||
+ | } | ||
+ | |||
+ | #bodyContent a[href ^="https://"], | ||
+ | .link-https { | ||
+ | padding-right: 35px; | ||
+ | } | ||
+ | |||
+ | .layui-nav * { | ||
+ | font-size: 18px; | ||
+ | } | ||
+ | |||
+ | .carousel-indicators { | ||
+ | |||
+ | width: 30%; | ||
+ | left: 33%; | ||
+ | } | ||
+ | |||
+ | .layui-carousel:hover .layui-carousel-arrow { | ||
+ | display: none; | ||
+ | } | ||
+ | |||
+ | .layui-carousel { | ||
+ | height: 1080px !important; | ||
+ | } | ||
+ | |||
+ | .big_demo{ | ||
+ | width:300px; | ||
+ | height:300px; | ||
+ | border-radius: 300px; | ||
+ | background-color:black; | ||
+ | |||
+ | } | ||
+ | .big_demo:hover{ | ||
+ | background-color:gray | ||
+ | } | ||
+ | .big-link{ | ||
+ | color: white; | ||
+ | font-size: 30px; | ||
+ | display: block; | ||
+ | padding: 100px; | ||
+ | } | ||
+ | .big-link:hover{ | ||
+ | text-decoration: none; | ||
+ | color:white; | ||
+ | } | ||
+ | </style> | ||
+ | |||
+ | <body> | ||
+ | <div style="height: 255px;width: 100%;display: flex;justify-content: space-around;"> | ||
+ | <img src="https://static.igem.org/mediawiki/2021/2/27/T--Shanghai_United--homepage01.png" | ||
+ | style="width: 173px; height: 89px;margin-right: 271px;margin-left: 120px;margin-top: 80px;display: block;" /> | ||
+ | <ul class="layui-nav navbar navbar-fixed-top text-center" lay-filter="" | ||
+ | style="background-color:rgb(0,0,0,0);height: 255px;line-height: 255px;"> | ||
+ | |||
+ | <li class="layui-nav-item" style="text-align: center;"> | ||
+ | <a href="https://2021.igem.org/Team:Shanghai_United" | ||
+ | style="text-align: center;">Home</a> | ||
+ | </li> | ||
+ | |||
+ | <li class="layui-nav-item" style="text-align: center;"> | ||
+ | <a href="javascript:;">Project</a> | ||
+ | <dl class="layui-nav-child"> | ||
+ | <!-- 二级菜单 --> | ||
+ | <dd> | ||
+ | <a | ||
+ | href="https://2021.igem.org/Team:Shanghai_United/Description">Description</a> | ||
+ | </dd> | ||
+ | <dd> | ||
+ | <a href="https://2021.igem.org/Team:Shanghai_United/Proof_Of_Concept">Proof | ||
+ | of Concept</a> | ||
+ | </dd> | ||
+ | <dd> | ||
+ | <a | ||
+ | href="https://2021.igem.org/Team:Shanghai_United/Experiments">Experiments</a> | ||
+ | </dd> | ||
+ | <dd> | ||
+ | <a | ||
+ | href="https://2021.igem.org/Team:Shanghai_United/Notebook">Notebook</a> | ||
+ | </dd> | ||
+ | <dd> | ||
+ | <a href="https://2021.igem.org/Team:Shanghai_United/Results">Results</a> | ||
+ | </dd> | ||
+ | <dd> | ||
+ | <a href="https://2021.igem.org/Team:Shanghai_United/Safety">Safety</a> | ||
+ | </dd> | ||
+ | |||
+ | </dl> | ||
+ | </li> | ||
+ | <li class="layui-nav-item" style="text-align: center;"> | ||
+ | <a href="javascript:;">Parts</a> | ||
+ | <dl class="layui-nav-child"> | ||
+ | <!-- 二级菜单 --> | ||
+ | <dd> | ||
+ | <a | ||
+ | href="https://2021.igem.org/Team:Shanghai_United/Engineering">Engineering</a> | ||
+ | </dd> | ||
+ | <dd> | ||
+ | <a href="https://2021.igem.org/Team:Shanghai_United/Parts">Parts | ||
+ | Collection</a> | ||
+ | </dd> | ||
+ | |||
+ | </dl> | ||
+ | </li> | ||
+ | <li class="layui-nav-item"> | ||
+ | <a href="javascript:;">Human Practice</a> | ||
+ | <dl class="layui-nav-child"> | ||
+ | <!-- 二级菜单 --> | ||
+ | <dd> | ||
+ | <a | ||
+ | href="https://2021.igem.org/Team:Shanghai_United/Fundraising">Fundraising</a> | ||
+ | </dd> | ||
+ | <dd> | ||
+ | <a | ||
+ | href="https://2021.igem.org/Team:Shanghai_United/Communication">Communication</a> | ||
+ | </dd> | ||
+ | <dd> | ||
+ | <a | ||
+ | href="https://2021.igem.org/Team:Shanghai_United/Human_Practices">Integrated Human Practices</a> | ||
+ | </dd> | ||
+ | </dl> | ||
+ | </li> | ||
+ | <li class="layui-nav-item" style="text-align: center;"> | ||
+ | <a href="https://2021.igem.org/Team:Shanghai_United/Implementation">Implementation</a> | ||
+ | </li> | ||
+ | |||
+ | <li class="layui-nav-item" style="text-align: center;"> | ||
+ | <a | ||
+ | href="https://2021.igem.org/Team:Shanghai_United/Entrepreneurship">Entrepreneurship</a> | ||
+ | </li> | ||
+ | |||
+ | <li class="layui-nav-item" style="text-align: center;"> | ||
+ | <a href="https://2021.igem.org/Team:Shanghai_United/Model">Model</a> | ||
+ | </li> | ||
+ | |||
+ | |||
+ | <li class="layui-nav-item" style="text-align: center;"> | ||
+ | <a href="javascript:;">Team</a> | ||
+ | <dl class="layui-nav-child"> | ||
+ | <!-- 二级菜单 --> | ||
+ | <dd> | ||
+ | <a href="https://2021.igem.org/Team:Shanghai_United/Team">Team | ||
+ | Member</a> | ||
+ | </dd> | ||
+ | <dd> | ||
+ | <a | ||
+ | href="https://2021.igem.org/Team:Shanghai_United/Collaborations">Collaborations</a> | ||
+ | </dd> | ||
+ | <dd> | ||
+ | <a | ||
+ | href="https://2021.igem.org/Team:Shanghai_United/Attributions">Attributions</a> | ||
+ | </dd> | ||
+ | </dl> | ||
+ | </li> | ||
+ | </ul> | ||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
</div> | </div> | ||
+ | <!--banner--> | ||
+ | <div style="width:100%;position: relative;"> | ||
+ | <img style="width:100%;" src="https://static.igem.org/mediawiki/2021/9/95/T--Shanghai_United--homepage08.jpg"></img> | ||
+ | <p style="color:white;font-size:48px;position: absolute;font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:48px; font-weight:bold;display: block;bottom: 100px;left: 100px;">Experiments</p> | ||
+ | </div> | ||
+ | |||
+ | <!--text--> | ||
+ | <div style="width:100%;padding:5% 30%;"> | ||
+ | <div style="display:flex;flex-direction: column;align-items:center;"> | ||
+ | <div style="display:flex;"> | ||
+ | <div class="big_demo" style="margin-right: 200px;"><a href="#" class="big-link" data-reveal-id="myModal1" data-animation="fade">Synthetic Biology Protocols</a></div> | ||
+ | <div class="big_demo"><a href="#" class="big-link" data-reveal-id="myModal2" data-animation="fade">General Protocols</a></div> | ||
+ | </div> | ||
+ | <div class="big_demo" style="margin-top: 100px;"><a href="#" class="big-link" data-reveal-id="myModal3" data-animation="fade">Function Test</a></div> | ||
+ | </div> | ||
+ | |||
+ | |||
+ | <div id="myModal1" class="reveal-modal"> | ||
+ | <div style="overflow:scroll;height: 700px;width:1000px"> | ||
+ | <div> | ||
+ | <h1 style="margin:7pt 0pt 0pt; orphans:0; text-indent:10pt; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:36px; font-weight:bold;">PCR of the target Gene</span> | ||
+ | </h1> | ||
+ | <p style="margin:7.05pt 0pt 0pt 28.2pt; orphans:0; text-indent:-19.2pt; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.05pt">A.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">      </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">Materials</span></p> | ||
+ | <p style="margin:0.3pt 0pt 0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:6pt; font-style:italic"> </span></p> | ||
+ | <table cellspacing="0" cellpadding="0" style="border-collapse:collapse; margin-left:10px auto"> | ||
+ | <tr style="height:36pt"> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:8.8pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">ddH</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:7.5pt">2</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">O</span></p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:8.95pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">32 μL</span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style="height:36pt"> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:8.9pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">dNTP</span></p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:8.9pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">5 μL</span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style="height:36pt"> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:8.85pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">MgSO</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:7.5pt">4</span></p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:9pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">4 μL</span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style="height:36pt"> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:8.95pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Forward</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">primer</span></p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:8.95pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">1 μL</span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style="height:36pt"> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:8.9pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Reverse</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">primer</span></p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:8.9pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">1 μL</span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style="height:36pt"> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:9pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">DNA</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.75pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">template</span></p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:9pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">1 μL</span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style="height:36pt"> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:8.95pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">KOD+</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">buffer</span></p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:8.95pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">5 μL</span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style="height:36pt"> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:8.9pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">KOD+</span></p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:8.9pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">1 μL</span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | </table> | ||
+ | <p style="margin:2.95pt 0pt 0pt 28.2pt; orphans:0; text-indent:-19.2pt; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.05pt">B.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">      </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">Protocol</span></p> | ||
+ | <p style="margin:7.1pt 0pt 0pt 46.4pt; orphans:0; text-indent:-19.25pt; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">1.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Prepare</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">and</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">mark</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">PCR</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">tubes</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">and</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">align tubes</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">in</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">order</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">on</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">tube</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">rack.</span></p> | ||
+ | <p | ||
+ | style="font-size:22px; line-height:130%; margin:7.05pt 47.05pt 0pt 46.4pt; orphans:0; text-indent:-19.2pt; widows:0"> | ||
+ | <span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">2.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">Under normal circumstances, | ||
+ | ddH</span><span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:7.5pt">2</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">O should be added into tubes | ||
+ | first, and</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.05pt; vertical-align:1pt"> | ||
+ | </span><span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt">enzymes should be | ||
+ | added </span><span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">last. All other materials | ||
+ | uncluding, but are not</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">limited</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.25pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">to</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">dNTP,</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.35pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">primers,</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">buffers,</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">and</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">templates</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">should</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">be</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.3pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">added</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">in</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">between.</span></p> | ||
+ | <p | ||
+ | style="font-size:22px; line-height:130%; margin:3.05pt 57.15pt 0pt 46.4pt; orphans:0; text-indent:-19.2pt; widows:0"> | ||
+ | <span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">3.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">PCR tubes should then be centrifuged briefly to | ||
+ | ensure all liquids collect at</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-2.85pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">the</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">bottom</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">of</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">the</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">tube.</span></p> | ||
+ | <p style="margin:3pt 0pt 0pt 46.4pt; orphans:0; text-indent:-19.25pt; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">4.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Move</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">PCR</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">tubes</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">to PCR</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">machine to initiate</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">the</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">process.</span></p> | ||
+ | <p style="margin:7.1pt 0pt 0pt 28.2pt; orphans:0; text-indent:-19.2pt; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.05pt">C.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">      </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">Reacting</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.1pt"> | ||
+ | </span><span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">conditions</span></p> | ||
+ | <p style="margin:0.3pt 0pt 0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:6pt; font-style:italic"> </span></p> | ||
+ | <table cellspacing="0" cellpadding="0" style="border-collapse:collapse; margin-left:6.5pt"> | ||
+ | <tr style="height:36pt"> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:149.75pt"> | ||
+ | <p style="margin:8.95pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">98 °C</span></p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:149.75pt"> | ||
+ | <p style="margin:8.95pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">10</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">minutes</span></p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:149.75pt"> | ||
+ | <p style="margin:8.95pt 0pt 0pt 2.9pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">1</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">cycle</span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style="height:36pt"> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:149.75pt"> | ||
+ | <p style="margin:9.05pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">98 °C</span></p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:149.75pt"> | ||
+ | <p style="margin:9.05pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">15</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">seconds</span></p> | ||
+ | </td> | ||
+ | <td rowspan="3" | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:149.75pt"> | ||
+ | <p style="margin:0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:13pt; font-style:italic"> </span></p> | ||
+ | <p style="margin:0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:13pt; font-style:italic"> </span></p> | ||
+ | <p style="margin:0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:14pt; font-style:italic"> </span></p> | ||
+ | <p style="margin:0pt 0pt 0pt 2.9pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">26</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">cycles</span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style="height:36pt"> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:149.75pt"> | ||
+ | <p style="margin:9pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">55 °C</span></p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:149.75pt"> | ||
+ | <p style="margin:9pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">15</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">seconds</span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style="height:35.85pt"> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:149.75pt"> | ||
+ | <p style="margin:8.95pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">68 °C</span></p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:149.75pt"> | ||
+ | <p style="margin:8.95pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">1 minute</span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style="height:45pt"> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:149.75pt"> | ||
+ | <p style="margin:0.3pt 0pt 0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:11.5pt; font-style:italic"> </span></p> | ||
+ | <p style="margin:0pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">68 °C</span></p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:149.75pt"> | ||
+ | <p style="margin:0.3pt 0pt 0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:11.5pt; font-style:italic"> </span></p> | ||
+ | <p style="margin:0pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">10</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">minutes</span></p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:149.75pt"> | ||
+ | <p style="margin:0.3pt 0pt 0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:11.5pt; font-style:italic"> </span></p> | ||
+ | <p style="margin:0pt 0pt 0pt 2.9pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">1</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">cycle</span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style="height:36pt"> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:149.75pt"> | ||
+ | <p style="margin:9.05pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">4 °C</span></p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:149.75pt"> | ||
+ | <p style="margin:9.05pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Infinite</span></p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:149.75pt"> | ||
+ | <p style="margin:9.05pt 0pt 0pt 2.9pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">N/A</span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | </table> | ||
+ | </div><br style="clear:both; mso-break-type:section-break; page-break-before:always" /> | ||
+ | <div> | ||
+ | <p style="margin:0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:10pt; font-style:italic"> </span></p> | ||
+ | <p style="margin:0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:10pt; font-style:italic"> </span></p> | ||
+ | <h1 style="margin:11.25pt 0pt 0pt 9pt; orphans:0; widows:0"> | ||
+ | <span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:36px; font-weight:bold;">Gel Electrophoresis of the Gene PCR Product | ||
+ | A. Materials</span></h1> | ||
+ | <p style="margin:0.3pt 0pt 0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:6pt; font-style:italic"> </span></p> | ||
+ | <table cellspacing="0" cellpadding="0" style="border-collapse:collapse; margin-left:6.5pt"> | ||
+ | <tr style="height:36pt"> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:8.95pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Loading</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">buffer</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">&</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">DNA</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.7pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">dye</span></p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:8.95pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">1 μL</span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style="height:36pt"> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:8.9pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">PCR</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">product</span></p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:8.9pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">5 μL</span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style="height:35.95pt"> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:9pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">TAE</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.65pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">buffer</span></p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:9pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">200 mL</span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style="height:36pt"> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:8.95pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Agarose</span></p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:8.95pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">2 g</span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | </table> | ||
+ | <p style="margin:2.95pt 0pt 0pt 28.2pt; orphans:0; text-indent:-19.2pt; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.05pt">B.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">      </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">Protocol</span></p> | ||
+ | <p | ||
+ | style="font-size:22px; line-height:130%; margin:7.2pt 56.55pt 0pt 51.1pt; orphans:0; text-indent:-19.2pt; widows:0"> | ||
+ | <span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">1.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt">Mix </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt">TAE buffer and agarose | ||
+ | powder in a ratio of 100 mL TAE buffer : 1 g</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-2.85pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">agarose powder.</span></p> | ||
+ | <p | ||
+ | style="font-size:22px; line-height:130%; margin:3pt 61.35pt 0pt 51.1pt; orphans:0; text-indent:-19.2pt; widows:0"> | ||
+ | <span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">2.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Heat the mixed solution in a microwave until | ||
+ | agarose powder completely</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-2.85pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">dissolves</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">into</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.25pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">TAE</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">buffer.</span></p> | ||
+ | <p style="margin:3pt 0pt 0pt 51pt; orphans:0; text-indent:-19.1pt; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">3.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Pour</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">the</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">liquid</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">into</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">gel</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">trays</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">of suitable</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">sizes.</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Insert</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">a</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">comb</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">onto</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">the</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">tray</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">to</span></p> | ||
+ | <p | ||
+ | style="font-size:22px; line-height:130%; margin:7.1pt 50.55pt 0pt 51.1pt; orphans:0; text-indent:-19.2pt; widows:0"> | ||
+ | <span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">4.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Mix</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">6x</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">loading</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">buffer</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">premixed</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">with</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.25pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">DNA</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.7pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">dye</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.2pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">with</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">PCR</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">product</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">in</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">a</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">ratio</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-2.85pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">of</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">1 unit loading buffer</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">: 5</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">units</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">PCR</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">product.</span></p> | ||
+ | <p style="margin:3pt 0pt 0pt 51pt; orphans:0; text-indent:-19.1pt; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">5.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Load</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">proper ladder</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">into</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">first</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">well</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">of</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">gel.</span></p> | ||
+ | <p style="margin:7.2pt 0pt 0pt 51pt; orphans:0; text-indent:-19.1pt; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">6.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Load</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">samples</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">into</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">wells.</span></p> | ||
+ | <p style="margin:7.05pt 0pt 0pt 51pt; orphans:0; text-indent:-19.1pt; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">7.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Perform</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">electrophoresis</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">at</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">185</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.3pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">V</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">for</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">10</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">minutes.</span></p> | ||
+ | <p style="margin:7.2pt 0pt 0pt 51pt; orphans:0; text-indent:-19.1pt; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">8.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Harvest</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">gel,</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">take</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">image</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">of</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">gel</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">under</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">untraviolet</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">light, and</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">analyze</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">results.</span></p> | ||
+ | <p style="margin:0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:13pt"> </span></p> | ||
+ | <p style="margin:0.5pt 0pt 0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:11pt"> </span></p> | ||
+ | <h1 style="margin:0pt 0pt 0pt 9pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:36px; font-weight:bold;">PCR Product Clean Up</span> | ||
+ | </h1> | ||
+ | <p style="margin:7.1pt 0pt 0pt 28.2pt; orphans:0; text-indent:-19.2pt; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.05pt">A.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">      </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">Protocol</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.25pt"> | ||
+ | </span><span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">(MN</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.1pt"> | ||
+ | </span><span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">NucleoSpin</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.1pt"> | ||
+ | </span><span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">Gel</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.05pt"> | ||
+ | </span><span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">and</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.1pt"> | ||
+ | </span><span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">PCR</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.15pt"> | ||
+ | </span><span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">Clean-up</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.1pt"> | ||
+ | </span><span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">Kit)</span></p> | ||
+ | <p style="margin:7.2pt 0pt 0pt 46.4pt; orphans:0; text-indent:-19.25pt; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">1.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Combine</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.3pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">obtained</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">PCR</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">products</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">into</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">one</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">centrifuge</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">tube.</span></p> | ||
+ | <p | ||
+ | style="font-size:22px; line-height:130%; margin:7.05pt 75.9pt 0pt 46.4pt; orphans:0; text-indent:-19.2pt; widows:0"> | ||
+ | <span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">2.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Add</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">buffer NTI</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">to</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">the</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">product</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">in</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">a ratio</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">of</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">2</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">μL</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.55pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">buffer NTI</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">:</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">1</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">μL</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.55pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">PCR</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-2.85pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">product</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">to allow</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">DNA</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.65pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">to bind</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">to the</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">membrane.</span></p> | ||
+ | <p style="margin:3pt 0pt 0pt 46.4pt; orphans:0; text-indent:-19.25pt; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">3.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Transfer</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">the</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">solution</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">to</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.25pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">a</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">column</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">placed within</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.25pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">a</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">collection</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">tube.</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Centrifuge</span></p> | ||
+ | </div><br style="clear:both; mso-break-type:section-break; page-break-before:always" /> | ||
+ | <div> | ||
+ | <p style="margin:4pt 0pt 0pt 46.4pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">at</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">12000</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">rpm</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">for 30 seconds.</span></p> | ||
+ | <p | ||
+ | style="font-size:22px; line-height:130%; margin:7.05pt 53.9pt 0pt 46.4pt; orphans:0; text-indent:-19.2pt; widows:0"> | ||
+ | <span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">4.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Transfer</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">the</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">liquid</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">in</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">the</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">collection tube</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">to</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">the</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">column.</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Centrifuge at</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">12000</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-2.85pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">rpm</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">for</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">30 seconds.</span></p> | ||
+ | <p | ||
+ | style="font-size:22px; line-height:130%; margin:3pt 53.2pt 0pt 46.4pt; orphans:0; text-indent:-19.2pt; widows:0"> | ||
+ | <span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">5.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Dispose</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">of</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">the liquid</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">in</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">the</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">collection</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">tube.</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.7pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Add</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">700</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">μL</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.55pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">buffer</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">NT3</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">to</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">wash</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-2.85pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">the</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">membrane. Centrifuge</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">at 12000</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">rpm for</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">30 seconds.</span></p> | ||
+ | <p style="margin:3pt 0pt 0pt 46.4pt; orphans:0; text-indent:-19.25pt; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">6.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Repeat step</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">5.</span></p> | ||
+ | <p | ||
+ | style="font-size:22px; line-height:131%; margin:7.05pt 44.45pt 0pt 46.4pt; orphans:0; text-indent:-19.2pt; widows:0"> | ||
+ | <span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">7.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">Add</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt; vertical-align:1pt"> | ||
+ | </span><span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">30</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt; vertical-align:1pt"> | ||
+ | </span><span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">μL</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.45pt; vertical-align:1pt"> | ||
+ | </span><span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">ddH</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:7.5pt">2</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">O</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt; vertical-align:1pt"> | ||
+ | </span><span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">preheated | ||
+ | to</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt; vertical-align:1pt"> | ||
+ | </span><span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">65</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt; vertical-align:1pt"> | ||
+ | </span><span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">˚C</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt; vertical-align:1pt"> | ||
+ | </span><span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">to</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt; vertical-align:1pt"> | ||
+ | </span><span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">the</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt; vertical-align:1pt"> | ||
+ | </span><span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">column.</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt; vertical-align:1pt"> | ||
+ | </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">Centrifuge</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.1pt; vertical-align:1pt"> | ||
+ | </span><span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">at</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt; vertical-align:1pt"> | ||
+ | </span><span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">12000</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt; vertical-align:1pt"> | ||
+ | </span><span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">rpm</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-2.85pt; vertical-align:1pt"> | ||
+ | </span><span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">for</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">30 seconds.</span></p> | ||
+ | <p style="margin:2.85pt 0pt 0pt 28.2pt; orphans:0; text-indent:-19.2pt; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.05pt">B.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">      </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">Buffers</span></p> | ||
+ | <p | ||
+ | style="font-size:22px; line-height:130%; margin:7.1pt 21.4pt 0pt 27.2pt; orphans:0; text-indent:24pt; widows:0"> | ||
+ | <span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt">Buffer NTI:</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.45pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt">Adjust</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt">DNA</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.6pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt">binding</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">conditions</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">and</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">allow</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">DNA</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.7pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">to</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">bind to</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-2.85pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">membrane.</span></p> | ||
+ | <p style="margin:3pt 0pt 0pt 51.2pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Buffer</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.4pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">NT3:</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.6pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Wash</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.3pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">membrane.</span></p> | ||
+ | <p style="margin:0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:13pt"> </span></p> | ||
+ | <p style="margin:0.45pt 0pt 0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:11pt"> </span></p> | ||
+ | <h1 style="margin:0.05pt 0pt 0pt 9pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:36px; font-weight:bold;">Plasmid Extraction</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-weight:bold; letter-spacing:-0.2pt;"> | ||
+ | </span> | ||
+ | </h1> | ||
+ | <p style="margin:7.05pt 0pt 0pt 28.2pt; orphans:0; text-indent:-19.2pt; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.05pt">A.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">      </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">Protocol</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.35pt"> | ||
+ | </span><span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">(TIAN</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.2pt"> | ||
+ | </span><span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">Prep</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.2pt"> | ||
+ | </span><span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">Mini</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.3pt"> | ||
+ | </span><span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">Plasmid</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.2pt"> | ||
+ | </span><span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">Kit)</span></p> | ||
+ | <p style="margin:7.2pt 0pt 0pt 46.4pt; orphans:0; text-indent:-19.25pt; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">1.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Harvest</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">and</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">transfer</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">cultivated bacteria</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">into</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">marked 2</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">mL</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.6pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">centrifuge tubes.</span></p> | ||
+ | <p | ||
+ | style="font-size:22px; line-height:130%; margin:7.2pt 40.1pt 0pt 46.4pt; orphans:0; text-indent:-19.2pt; widows:0"> | ||
+ | <span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">2.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Centrifuge tubes at 12000 rpm for 30 seconds to | ||
+ | deposit bacteria to the bottom</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-2.85pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">of</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">the</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">tube.</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Dispose</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">of</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">the</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">clear</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">liquid on top.</span></p> | ||
+ | <p | ||
+ | style="font-size:22px; line-height:130%; margin:2.9pt 55.3pt 0pt 46.4pt; orphans:0; text-indent:-19.2pt; widows:0"> | ||
+ | <span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">3.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Add</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">250</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">μL</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.5pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">buffer</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">P1</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">into</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">the</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">2</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">mL</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.55pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">centrifuge tubes</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">to</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">levitete</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">the</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">bacteria</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-2.85pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">persent</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">in</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">the</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">tubes.</span></p> | ||
+ | <p | ||
+ | style="font-size:22px; line-height:130%; margin:3pt 45.4pt 0pt 46.4pt; orphans:0; text-indent:-19.2pt; widows:0"> | ||
+ | <span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">4.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Add</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">250</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">μL</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.45pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">buffer</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">P2</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">to</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">the</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">t</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">ubes</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">to</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">lyse bacterial</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">cell</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">wall.</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Mix</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.25pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">the</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">solution</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-2.85pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">gently;</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.2pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">do not</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">vortex.</span></p> | ||
+ | <p | ||
+ | style="font-size:22px; line-height:130%; margin:3pt 51.65pt 0pt 46.4pt; orphans:0; text-indent:-19.2pt; widows:0"> | ||
+ | <span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">5.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Add</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">350</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">μL</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.45pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">buffer</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">P3</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">to</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">the</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">tubes</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">to</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">neutralize</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">buffer</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">P2</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.25pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">and</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">cause</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">proteins</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-2.85pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">in</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">the</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">solution</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">to percipitate.</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Mix</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">the solution</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">gently;</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.2pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">do not</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">vortex.</span></p> | ||
+ | <p | ||
+ | style="font-size:22px; line-height:130%; margin:3pt 43.8pt 0pt 46.4pt; orphans:0; text-indent:-19.2pt; widows:0"> | ||
+ | <span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">6.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Centrifuge the solution at 12000 rpm for 10 | ||
+ | minutes to collect percitipation to</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-2.85pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">the</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">bottom</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">of</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">the</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">tube.</span></p> | ||
+ | <p | ||
+ | style="font-size:22px; line-height:130%; margin:3.15pt 42.1pt 0pt 46.4pt; orphans:0; text-indent:-19.2pt; widows:0"> | ||
+ | <span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">7.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Add</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">500</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">μL</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.45pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">buffer</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">BL</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.3pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">to</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">a</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">column</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">placed within</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">a collection</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">tube</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">to</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">wash</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">the</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-2.85pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">membrane.</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Centrifuge</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">at 12000 rpm</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">for</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">30 seconds.</span></p> | ||
+ | <p | ||
+ | style="font-size:22px; line-height:130%; margin:2.85pt 42.6pt 0pt 46.4pt; orphans:0; text-indent:-19.2pt; widows:0"> | ||
+ | <span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">8.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Transfer</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">the</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">clear liquid</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">obtained</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">in</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">step</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">6</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">to</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">the</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">column</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">washed</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">in</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.25pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">step</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">7</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">and</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-2.85pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">centrifuge the</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">column at</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">12000 rpm</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">for</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">30 seconds.</span></p> | ||
+ | <p | ||
+ | style="font-size:22px; line-height:130%; margin:3pt 56.35pt 0pt 46.4pt; orphans:0; text-indent:-19.2pt; widows:0"> | ||
+ | <span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">9.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Dispose</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">the</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">clear liquid</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">in</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">the</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">collection</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">tube.</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.65pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Add</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">500</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">μL</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.45pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">buffer</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">PD</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">to</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">the</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-2.85pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">column</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">and centrifuge</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">at 12000</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">rpm for</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">30 seconds.</span></p> | ||
+ | <p | ||
+ | style="font-size:22px; line-height:130%; margin:3pt 67.3pt 0pt 46.4pt; orphans:0; text-indent:-19.2pt; widows:0"> | ||
+ | <span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">10.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">   </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Dispose</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">of</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">the</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">liquid</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">in</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">the</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">collection</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">tube.</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.6pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Add</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">600</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">μL</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.55pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">buffer PW</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.5pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">to</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">the</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-2.85pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">column.</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Centrifuge</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">at 12000</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">rpm for</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">30 seconds.</span></p> | ||
+ | <p style="margin:3pt 0pt 0pt 46.4pt; orphans:0; text-indent:-19.25pt; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">11.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">   </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Repeat step</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">10.</span></p> | ||
+ | </div><br style="clear:both; mso-break-type:section-break; page-break-before:always" /> | ||
+ | <div> | ||
+ | <p | ||
+ | style="font-size:22px; line-height:130%; margin:4pt 45.8pt 0pt 46.4pt; orphans:0; text-indent:-19.2pt; widows:0"> | ||
+ | <span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">12.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">   </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Dispose of the clear liquid in the collection | ||
+ | tube. Centrifuge at 12000 rpm for</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-2.85pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">1 minute.</span></p> | ||
+ | <p | ||
+ | style="font-size:22px; line-height:130%; margin:3pt 44.8pt 0pt 46.4pt; orphans:0; text-indent:-19.2pt; widows:0"> | ||
+ | <span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">13.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">   </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Transfer</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">the</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">column</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">to</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">a</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">new</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">2</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">mL</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.55pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">centrifiuge</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">tube.</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Dispose</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">of</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">the</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">collection</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-2.85pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">tube.</span></p> | ||
+ | <p | ||
+ | style="font-size:22px; line-height:131%; margin:2.8pt 43.7pt 0pt 46.4pt; orphans:0; text-indent:-19.2pt; widows:0"> | ||
+ | <span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">14.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">   </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">Add</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt; vertical-align:1pt"> | ||
+ | </span><span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">40</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt; vertical-align:1pt"> | ||
+ | </span><span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">μL</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.4pt; vertical-align:1pt"> | ||
+ | </span><span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">ddH</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:7.5pt">2</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">O</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt; vertical-align:1pt"> | ||
+ | </span><span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">preheated</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.05pt; vertical-align:1pt"> | ||
+ | </span><span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">to</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt; vertical-align:1pt"> | ||
+ | </span><span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">65</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt; vertical-align:1pt"> | ||
+ | </span><span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">°C</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt; vertical-align:1pt"> | ||
+ | </span><span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">to</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt; vertical-align:1pt"> | ||
+ | </span><span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">the | ||
+ | column.</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt; vertical-align:1pt"> | ||
+ | </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">Centrifuge</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt; vertical-align:1pt"> | ||
+ | </span><span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">at</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt; vertical-align:1pt"> | ||
+ | </span><span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">12000</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt; vertical-align:1pt"> | ||
+ | </span><span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">rpm</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-2.85pt; vertical-align:1pt"> | ||
+ | </span><span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">for</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">30 seconds.</span></p> | ||
+ | <p style="margin:2.9pt 0pt 0pt 28.2pt; orphans:0; text-indent:-19.2pt; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.05pt">B.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">      </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">Buffers</span></p> | ||
+ | <p style="font-size:22px; line-height:152%; margin:7.05pt 207.7pt 0pt 48.2pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Buffer P1: Levitate bacteria and lyse | ||
+ | RNA.</span><span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-2.9pt"> | ||
+ | </span><span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Buffer</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">P2:</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Lyse</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">bacteria</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">cell</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">wall.</span></p> | ||
+ | <p style="font-size:22px; line-height:151%; margin:0pt 136.65pt 0pt 48.2pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Buffer</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">P3:</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Neutralize</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">P2</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">and</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">cause</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">proteins</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">to</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">precipitate.</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-2.85pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Buffer</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">BL:</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Wash</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">memnbrane pre-use.</span></p> | ||
+ | <p style="font-size:22px; line-height:152%; margin:0.15pt 201.15pt 0pt 48.2pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Buffer</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.35pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">PD:</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.55pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Wash</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.25pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">proteins</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.3pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">off</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.35pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">the</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.35pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">membrane.</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-2.85pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Buffer</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.25pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">PW:</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.65pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Wash</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">salts</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">off</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.4pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">the</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">membrane.</span></p> | ||
+ | <p style="margin:0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:13pt"> </span></p> | ||
+ | <p style="margin:0.35pt 0pt 0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:12.5pt"> </span></p> | ||
+ | <h1 style="margin:0pt 0pt 0pt 9pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:36px; font-weight:bold;">Restriction Enzyme Digestion</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-weight:bold; letter-spacing:-0.15pt;"> | ||
+ | </span> | ||
+ | </h1> | ||
+ | <p style="margin:7.1pt 0pt 0pt 28.2pt; orphans:0; text-indent:-19.2pt; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.05pt">A.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">      </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">Materials</span></p> | ||
+ | <p style="margin:0.35pt 0pt 0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:6pt; font-style:italic"> </span></p> | ||
+ | <table cellspacing="0" cellpadding="0" style="border-collapse:collapse; margin-left:6.5pt"> | ||
+ | <tr style="height:36pt"> | ||
+ | <td colspan="2" | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:450.5pt"> | ||
+ | <p style="margin:8.9pt 172.6pt 0pt 173.85pt; orphans:0; text-align:center; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">ARSA/ARSR/ARSD</span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style="height:36pt"> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:9pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">10x</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">NEB</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">buffer</span></p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:9pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">5 μL</span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style="height:36pt"> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:8.95pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">ARSA/D/R</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.25pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Plasmid</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.25pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">(300</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">ng/μL)</span></p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:8.95pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">10 μL</span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style="height:36pt"> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:8.9pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">BsaI</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">restriction</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">enzyme</span></p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:8.9pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">1 μL</span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style="height:36pt"> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:8.85pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">ddH</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:7.5pt">2</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">O</span></p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:9pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">34 μL</span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style="height:36pt"> | ||
+ | <td colspan="2" | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:450.5pt"> | ||
+ | <p style="margin:8.95pt 172.6pt 0pt 173.55pt; orphans:0; text-align:center; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">amilGFP</span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style="height:36pt"> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:8.9pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">10x</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">NEB</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">buffer</span></p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:8.9pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">5 μL</span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style="height:36pt"> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:9pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">amilGFP</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.6pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">(400</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">ng/μL)</span></p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:9pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">10 μL</span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style="height:35.85pt"> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:8.95pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">BsaI</span></p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:8.95pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">1 μL</span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style="height:0pt"> | ||
+ | <td style="width:225.75pt; border:none"></td> | ||
+ | <td style="width:225.75pt; border:none"></td> | ||
+ | </tr> | ||
+ | <tr style="height:36pt"> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:8.95pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">DpnI</span></p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:8.95pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">1 μL</span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style="height:45pt"> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:0.2pt 0pt 0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:11.5pt; font-style:italic"> </span></p> | ||
+ | <p style="margin:0.05pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">ddH</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:7.5pt">2</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">O</span></p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:0.25pt 0pt 0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:11.5pt; font-style:italic"> </span></p> | ||
+ | <p style="margin:0pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">33 μL</span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | </table> | ||
+ | </div><br style="clear:both; mso-break-type:section-break; page-break-before:always" /> | ||
+ | <div> | ||
+ | <p style="margin:3.05pt 0pt 0pt 28.2pt; orphans:0; text-indent:-19.2pt; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.05pt">B.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">      </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">Protocol</span></p> | ||
+ | <p style="margin:7.05pt 0pt 0pt 46.4pt; orphans:0; text-indent:-19.25pt; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">1.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Mix</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">the</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">materials</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">in</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">a 1.5</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">mL</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.55pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">centrifuge tube.</span></p> | ||
+ | <p style="margin:7.2pt 0pt 0pt 46.4pt; orphans:0; text-indent:-19.25pt; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">2.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Place</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">the</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">centrifuge</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">tube</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">in</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">37</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">°C</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">metal</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">bath</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">for</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">at</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">least</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">15</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">minutes.</span></p> | ||
+ | <p style="margin:0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:13pt"> </span></p> | ||
+ | <p style="margin:0.5pt 0pt 0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:11pt"> </span></p> | ||
+ | <h1 style="margin:0pt 0pt 0pt 9pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:36px; font-weight:bold;">Gel Electrophoresis of Restriction Enzyme Digestion Product</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-weight:bold; letter-spacing:-0.25pt;"> | ||
+ | </span> | ||
+ | </h1> | ||
+ | <p style="margin:7.05pt 0pt 0pt 28.2pt; orphans:0; text-indent:-19.2pt; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.05pt">A.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">      </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">Materials</span></p> | ||
+ | <p style="margin:0.3pt 0pt 0.05pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:6pt; font-style:italic"> </span></p> | ||
+ | <table cellspacing="0" cellpadding="0" style="border-collapse:collapse; margin-left:6.5pt"> | ||
+ | <tr style="height:42.75pt"> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:0.25pt 0pt 0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:10.5pt; font-style:italic"> </span></p> | ||
+ | <p style="margin:0pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Loading</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">buffer</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">with</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">DNA</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.65pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">dye</span></p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:0.25pt 0pt 0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:10.5pt; font-style:italic"> </span></p> | ||
+ | <p style="margin:0pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">10 μL</span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style="height:42.75pt"> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:0.3pt 0pt 0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:10.5pt; font-style:italic"> </span></p> | ||
+ | <p style="margin:0pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Digestion</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">product</span></p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:0.3pt 0pt 0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:10.5pt; font-style:italic"> </span></p> | ||
+ | <p style="margin:0pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">50 μL</span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | </table> | ||
+ | <p style="margin:2.9pt 0pt 0pt 28.2pt; orphans:0; text-indent:-19.2pt; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.05pt">B.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">      </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">Protocol</span></p> | ||
+ | <p | ||
+ | style="font-size:22px; line-height:130%; margin:7.2pt 42pt 0pt 46.4pt; orphans:0; text-indent:-19.2pt; widows:0"> | ||
+ | <span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">1.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Mix</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">6x</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">loading</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">buffer</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">premixed</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">with</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">DNA</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.7pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">dye</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.2pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">with</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">PCR</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">product</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">in</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">a</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">ratio of</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-2.85pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">1</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">unit</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">loading buffer</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">:</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">5 units PCR</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">product.</span></p> | ||
+ | <p style="margin:2.9pt 0pt 0pt 46.4pt; orphans:0; text-indent:-19.25pt; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">2.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Load</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">proper ladder</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">into</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">first</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">well</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">of</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">gel.</span></p> | ||
+ | <p style="margin:7.2pt 0pt 0pt 46.4pt; orphans:0; text-indent:-19.25pt; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">3.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Load</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">samples</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">into</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.25pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">wells.</span></p> | ||
+ | <p style="margin:7.2pt 0pt 0pt 46.4pt; orphans:0; text-indent:-19.25pt; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">4.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Perform electrophoresis</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">at</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">185</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.3pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">V</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">for</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">10</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">minutes.</span></p> | ||
+ | <p style="margin:7.1pt 0pt 0pt 46.4pt; orphans:0; text-indent:-19.25pt; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">5.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Harvest</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">gel,</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">take</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">image</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">of</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">gel</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">under</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">untraviolet</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">light, and</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">analyze</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">results.</span></p> | ||
+ | <p style="margin:0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:13pt"> </span></p> | ||
+ | <p style="margin:0.45pt 0pt 0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:11pt"> </span></p> | ||
+ | <h1 style="margin:0pt 0pt 0pt 9pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:36px; font-weight:bold;">Agarose Gel DNA Extraction</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-weight:bold; letter-spacing:-0.2pt;"> | ||
+ | </span> | ||
+ | </h1> | ||
+ | <p style="margin:7.1pt 0pt 0pt 28.2pt; orphans:0; text-indent:-19.2pt; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.05pt">A.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">      </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">Protocol</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.25pt"> | ||
+ | </span><span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">(MN</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.1pt"> | ||
+ | </span><span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">NucleoSpin</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.1pt"> | ||
+ | </span><span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">Gel</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.05pt"> | ||
+ | </span><span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">and</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.1pt"> | ||
+ | </span><span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">PCR</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.15pt"> | ||
+ | </span><span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">Clean-up</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.1pt"> | ||
+ | </span><span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">Kit)</span></p> | ||
+ | <p | ||
+ | style="font-size:22px; line-height:130%; margin:7.2pt 57.5pt 0pt 46.4pt; orphans:0; text-indent:-19.2pt; widows:0"> | ||
+ | <span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">1.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt">Collect gel with desired | ||
+ | DNA fragments into 2 mL </span><span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">centrifuge | ||
+ | tubes. Weigh</span><span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-2.85pt"> | ||
+ | </span><span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">the</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">mass of</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">gels on an</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">electronic balance.</span></p> | ||
+ | <p style="margin:3pt 0pt 0pt 46.4pt; orphans:0; text-indent:-19.25pt; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">2.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Add</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">buffer</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">NTI</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">to</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">the</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">tube</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">in</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">a</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">ratio</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">of</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">2</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">μL</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.45pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">buffer</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">NTI :</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">1</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">mg</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">agarose gel.</span></p> | ||
+ | <p style="margin:7.1pt 0pt 0pt 46.4pt; orphans:0; text-indent:-19.25pt; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">3.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Heat the</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">mixture</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">at</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">50</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">˚C</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">in</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">a</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">metal</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">bath</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">until</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">the</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">gel</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">dissolves</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">completely.</span></p> | ||
+ | <p | ||
+ | style="font-size:22px; line-height:130%; margin:7.2pt 82.35pt 0pt 46.4pt; orphans:0; text-indent:-19.2pt; widows:0"> | ||
+ | <span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">4.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Transfer</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">the</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">solution</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">to</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.25pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">a</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">new</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">column</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">placed within</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">a</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">collection</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">tube.</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-2.85pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Centrifuge at 12000 rpm for</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">30 seconds.</span></p> | ||
+ | <p | ||
+ | style="font-size:22px; line-height:130%; margin:3pt 53.9pt 0pt 46.4pt; orphans:0; text-indent:-19.2pt; widows:0"> | ||
+ | <span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">5.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Transfer</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">the</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">liquid</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">in</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">the</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">collection tube</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">to</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">the</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">column.</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Centrifuge at</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">12000</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-2.85pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">rpm</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">for</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">30 seconds.</span></p> | ||
+ | <p style="margin:3pt 0pt 0pt 46.4pt; orphans:0; text-indent:-19.25pt; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">6.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Dispose</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">of</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">the clear liquid</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">in</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">the</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">collection</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">tube.</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.6pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Add</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">700</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">μL</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.55pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">buffer NT3</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">to</span></p> | ||
+ | </div><br style="clear:both; mso-break-type:section-break; page-break-before:always" /> | ||
+ | <div> | ||
+ | <p style="margin:4pt 0pt 0pt 46.4pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">the</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">column.</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Centrifuge at</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">12000</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">rpm</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">for 30</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">seconds.</span></p> | ||
+ | <p style="margin:7.05pt 0pt 0pt 46.4pt; orphans:0; text-indent:-19.25pt; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">7.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Repeat step</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">6.</span></p> | ||
+ | <p | ||
+ | style="font-size:22px; line-height:130%; margin:7.2pt 63.15pt 0pt 46.4pt; orphans:0; text-indent:-19.2pt; widows:0"> | ||
+ | <span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">8.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Dispose of the liquid in the collection tube. | ||
+ | Centrifuge at 12000 rpm for 1</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-2.85pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">minute.</span></p> | ||
+ | <p | ||
+ | style="font-size:22px; line-height:130%; margin:3pt 42.15pt 0pt 46.4pt; orphans:0; text-indent:-19.2pt; widows:0"> | ||
+ | <span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">9.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Add</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">30</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">μL</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.45pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">ddH2O prehe</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">ated</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">to</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">65</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">˚C</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">to</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">the</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">column.</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Centrifuge</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">at</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">12000</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">rpm</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-2.85pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">for</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">30 seconds.</span></p> | ||
+ | <p style="margin:0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:13pt"> </span></p> | ||
+ | <h1 style="margin:8.95pt 0pt 0pt 9pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:36px; font-weight:bold; letter-spacing:-0.05pt;">T4 DNA Ligase Ligation</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-weight:bold;"> | ||
+ | </span></h1> | ||
+ | <p style="margin:7.2pt 0pt 0pt 28.2pt; orphans:0; text-indent:-19.2pt; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.05pt">A.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">      </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">Materials</span></p> | ||
+ | <p style="margin:0.2pt 0pt 0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:6pt; font-style:italic"> </span></p> | ||
+ | <table cellspacing="0" cellpadding="0" style="border-collapse:collapse; margin-left:6.5pt"> | ||
+ | <tr style="height:36pt"> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:8.9pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">ARSA/R/D</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.3pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">plasmid</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.25pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">(30 ng/μL)</span></p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:8.9pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">1.5 μL</span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style="height:36pt"> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:9pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">GFP</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.5pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">(100</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">ng/μL)</span></p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:9pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">4.5 μL</span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style="height:45pt"> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:0.15pt 0pt 0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:11.5pt; font-style:italic"> </span></p> | ||
+ | <p style="margin:0.05pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">T4</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">DNA</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.7pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">ligase</span></p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:0.15pt 0pt 0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:11.5pt; font-style:italic"> </span></p> | ||
+ | <p style="margin:0.05pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">1 μL</span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style="height:36pt"> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:8.9pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt">T4 | ||
+ | DNA</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.65pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt">ligase</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px"> buffer</span></p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:8.9pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">1 μL</span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style="height:36pt"> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:8.85pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">ddH</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:7.5pt">2</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">O</span></p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:9pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">2 μL</span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | </table> | ||
+ | <p style="margin:2.95pt 0pt 0pt 28.2pt; orphans:0; text-indent:-19.2pt; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.05pt">B.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">      </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">Protocol</span></p> | ||
+ | <p | ||
+ | style="font-size:22px; line-height:130%; margin:7.05pt 46.2pt 0pt 46.4pt; orphans:0; text-indent:-19.2pt; widows:0"> | ||
+ | <span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">1.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Add</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">ddH2O,</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.25pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">T4</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">DNA</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.75pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">ligase</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">buffer,</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.25pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">and</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.35pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">T4</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">DNA</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.7pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">ligase into</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.25pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">a</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">PCR</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.35pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">tube</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">with</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-2.85pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt">GFP gene and ARS plasmid | ||
+ | </span><span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">in a ratio of 1 units ARS plasmid : 10 | ||
+ | unit GFP</span><span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.05pt"> | ||
+ | </span><span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">gene.</span></p> | ||
+ | <p style="margin:3.1pt 0pt 0pt 46.4pt; orphans:0; text-indent:-19.25pt; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">2.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Transfer</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">the</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">solution</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">to</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.25pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">PCR</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">machine</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">at</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">16˚C.</span></p> | ||
+ | <p style="margin:0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:13pt"> </span></p> | ||
+ | <p style="margin:0.45pt 0pt 0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:11pt"> </span></p> | ||
+ | <h1 style="margin:0pt 0pt 0pt 9pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:36px; font-weight:bold;">Transformation of DH5-α E. coli Using Heat Shock</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-weight:bold; letter-spacing:-0.15pt;"> | ||
+ | </span> | ||
+ | </h1> | ||
+ | <p style="margin:7.2pt 0pt 0pt 28.2pt; orphans:0; text-indent:-19.2pt; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.05pt">A.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">      </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">Protocol</span></p> | ||
+ | <p | ||
+ | style="font-size:22px; line-height:130%; margin:7.1pt 45.85pt 0pt 46.4pt; orphans:0; text-indent:-19.2pt; widows:0"> | ||
+ | <span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">1.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Mix the tube introduced DNA material with DH5-α | ||
+ | competent cells. The</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">volumn</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.25pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">of DNA</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.65pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">material must</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">not</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">exceed</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">10%</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">of that</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">of</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">the</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">competent</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">cells.</span></p> | ||
+ | <p | ||
+ | style="font-size:22px; line-height:130%; margin:3pt 50.55pt 0pt 46.4pt; orphans:0; text-indent:-19.2pt; widows:0"> | ||
+ | <span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">2.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Gently</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">fick</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">the</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">tube</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">4-5</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">times</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">to</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">mix</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">competent</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">cells</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">and</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">DNA</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.7pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">material. Do</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-2.85pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">not vortex.</span></p> | ||
+ | <p style="margin:3pt 0pt 0pt 46.4pt; orphans:0; text-indent:-19.25pt; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">3.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Place</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">the</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">tube</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">on</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">ice</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">for 30</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">minutes.</span></p> | ||
+ | <p style="margin:7.1pt 0pt 0pt 46.4pt; orphans:0; text-indent:-19.25pt; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">4.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Place</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">the</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">tube at</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">exactly</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">42</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">˚C</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">for</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">90</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">seconds</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">to</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">perform</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">heat</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">shock.</span></p> | ||
+ | <p | ||
+ | style="font-size:22px; line-height:130%; margin:7.2pt 46.3pt 0pt 46.4pt; orphans:0; text-indent:-19.2pt; widows:0"> | ||
+ | <span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">5.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Immediately place the tube back on ice after heat | ||
+ | shock. Keep the tube on ice</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-2.85pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">for</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">2-3</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">minutes.</span></p> | ||
+ | </div><br style="clear:both; mso-break-type:section-break; page-break-before:always" /> | ||
+ | <div> | ||
+ | <p | ||
+ | style="font-size:22px; line-height:130%; margin:4pt 76.45pt 0pt 46.4pt; orphans:0; text-indent:-19.2pt; widows:0"> | ||
+ | <span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">6.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Add</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">900</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">μL</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.5pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">LB</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">culture</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">medium</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">without</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">KANA</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.7pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">antibiotics</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">to</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">the</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">tube.</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-2.85pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Incubate</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">the</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">transformed</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">bacteria</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">at 37</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">˚C for</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">30</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">minutes.</span></p> | ||
+ | <p style="margin:3pt 0pt 0pt 46.4pt; orphans:0; text-indent:-19.25pt; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">7.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Centrifuge the</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">tube</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">at</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">12000</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">rpm</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">for</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">30</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">seconds.</span></p> | ||
+ | <p | ||
+ | style="font-size:22px; line-height:130%; margin:7.05pt 61.9pt 0pt 46.4pt; orphans:0; text-indent:-19.2pt; widows:0"> | ||
+ | <span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">8.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Dispose half of the clear liquid in the centrifuge | ||
+ | tube. Mix and levitate the</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-2.85pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">bacteria</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">on the</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">bottom of</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">the</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">tube</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">with a</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">micropipette.</span></p> | ||
+ | <p style="margin:3pt 0pt 0pt 30pt; orphans:0; text-indent:-21pt; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">9.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">        </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">In</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">a</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">sterile</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">environment,</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">transfer</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">the</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">transformed bacteria</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">to</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">prepared agar</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">plates.</span></p> | ||
+ | <p style="margin:0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:13pt"> </span></p> | ||
+ | <p style="margin:0.5pt 0pt 0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:11pt"> </span></p> | ||
+ | <h1 style="margin:0pt 0pt 0pt 9pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:36px; font-weight:bold;">Colony PCR Identification</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-weight:bold; letter-spacing:-0.3pt;"> | ||
+ | </span> | ||
+ | </h1> | ||
+ | <p style="margin:7.2pt 0pt 0pt 30pt; orphans:0; text-indent:-21pt; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.05pt">A.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">Materials</span></p> | ||
+ | <p style="margin:0.2pt 0pt 0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:6pt; font-style:italic"> </span></p> | ||
+ | <table cellspacing="0" cellpadding="0" style="border-collapse:collapse; margin-left:6.5pt"> | ||
+ | <tr style="height:36pt"> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:8.9pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">2x PCR</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">mix</span></p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:8.9pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">10 μL</span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style="height:36pt"> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:9pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Forward</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">primer</span></p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:9pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">0.5 μL</span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style="height:36pt"> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:8.95pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Reverse</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">primer</span></p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:8.95pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">0.5 μL</span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style="height:36pt"> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:8.9pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Template</span></p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:8.9pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Desired </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">E.</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.1pt"> | ||
+ | </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">Coli</span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style="height:36pt"> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:8.85pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">ddH</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:7.5pt">2</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">O</span></p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:9pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">9 μL</span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | </table> | ||
+ | <p style="margin:2.95pt 0pt 0pt 28.2pt; orphans:0; text-indent:-19.2pt; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.05pt">B.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">      </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">Protocol</span></p> | ||
+ | <p style="margin:7.05pt 0pt 0pt 46.4pt; orphans:0; text-indent:-19.25pt; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">1.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Refer</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">to</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">PCR</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.2pt"> | ||
+ | </span><span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">of</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.05pt"> | ||
+ | </span><span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">amilGFP</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.2pt"> | ||
+ | </span><span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">Gene, Jul.</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.05pt"> | ||
+ | </span><span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">28,</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.05pt"> | ||
+ | </span><span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">2021</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">.</span></p> | ||
+ | <p style="margin:7.2pt 0pt 0pt 46.4pt; orphans:0; text-indent:-19.25pt; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">2.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Notes</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">to</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">this</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">reaction:</span></p> | ||
+ | <p | ||
+ | style="font-size:22px; line-height:130%; margin:7.25pt 50.3pt 0pt 67.4pt; orphans:0; text-indent:-19.2pt; widows:0"> | ||
+ | <span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal; letter-spacing:-0.05pt">a.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Primers</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">were</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">delivered</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">in</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">the</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">form</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">of</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">dry</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">powder.</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.3pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Centrifuge</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">primers</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">at</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-2.85pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">12000</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">rpm</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">for</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">5</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">minutes to</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">deposit powder at the</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">bottom</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">of its</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">tube.</span></p> | ||
+ | <p style="margin:2.85pt 0pt 0pt 67.4pt; orphans:0; text-indent:-19.25pt; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal; letter-spacing:-0.05pt">b.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Dilute</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">primers</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">to</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">a concentration</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">of</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">10</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">nmol/ml.</span></p> | ||
+ | <p style="margin:7.2pt 0pt 0pt 67.4pt; orphans:0; text-indent:-19.25pt; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal; letter-spacing:-0.05pt">c.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Mix</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">the</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">2x</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">PCR</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.25pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">mix,</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.25pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">R.primer,</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.55pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Templalate,</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">ddH2O</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">as</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">master</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">mix.</span></p> | ||
+ | <p style="margin:7.2pt 0pt 0pt 28.2pt; orphans:0; text-indent:-19.2pt; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.05pt">C.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">      </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">Reacting</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.1pt"> | ||
+ | </span><span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">conditions</span></p> | ||
+ | <p style="margin:0.2pt 0pt 0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:6pt; font-style:italic"> </span></p> | ||
+ | <table cellspacing="0" cellpadding="0" style="border-collapse:collapse; margin-left:6.5pt"> | ||
+ | <tr style="height:36pt"> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:149.75pt"> | ||
+ | <p style="margin:8.95pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">98 °C</span></p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:149.75pt"> | ||
+ | <p style="margin:8.95pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">10</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">minutes</span></p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:149.75pt"> | ||
+ | <p style="margin:8.95pt 0pt 0pt 2.9pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">1</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">cycle</span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style="height:36pt"> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:149.75pt"> | ||
+ | <p style="margin:9pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">98 °C</span></p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:149.75pt"> | ||
+ | <p style="margin:9pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">15</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">seconds</span></p> | ||
+ | </td> | ||
+ | <td rowspan="3" | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:149.75pt"> | ||
+ | <p style="margin:0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:13pt; font-style:italic"> </span></p> | ||
+ | <p style="margin:0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:13pt; font-style:italic"> </span></p> | ||
+ | <p style="margin:0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:14pt; font-style:italic"> </span></p> | ||
+ | <p style="margin:0pt 0pt 0pt 2.9pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">26</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">cycles</span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style="height:36pt"> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:149.75pt"> | ||
+ | <p style="margin:9pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">55 °C</span></p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:149.75pt"> | ||
+ | <p style="margin:9pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">15</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">seconds</span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style="height:35.9pt"> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:149.75pt"> | ||
+ | <p style="margin:8.95pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">72 °C</span></p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:149.75pt"> | ||
+ | <p style="margin:8.95pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">30</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">seconds</span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style="height:45pt"> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:149.75pt"> | ||
+ | <p style="margin:0.3pt 0pt 0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:11.5pt; font-style:italic"> </span></p> | ||
+ | <p style="margin:0pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">72 °C</span></p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:149.75pt"> | ||
+ | <p style="margin:0.3pt 0pt 0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:11.5pt; font-style:italic"> </span></p> | ||
+ | <p style="margin:0pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">5</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">minutes</span></p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:149.75pt"> | ||
+ | <p style="margin:0.3pt 0pt 0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:11.5pt; font-style:italic"> </span></p> | ||
+ | <p style="margin:0pt 0pt 0pt 2.9pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">1</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">cycle</span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style="height:36pt"> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:149.75pt"> | ||
+ | <p style="margin:9.05pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">4 °C</span></p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:149.75pt"> | ||
+ | <p style="margin:9.05pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Infinite</span></p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:149.75pt"> | ||
+ | <p style="margin:9.05pt 0pt 0pt 2.9pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">N/A</span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | </table> | ||
+ | </div><br style="clear:both; mso-break-type:section-break; page-break-before:always" /> | ||
+ | <div> | ||
+ | <p style="margin:0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:11pt"> </span></p> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div id="myModal2" class="reveal-modal"> | ||
+ | <div style="overflow:scroll;height: 700px;width:1000px"> | ||
+ | <div> | ||
+ | <h1 style="margin:7pt 0pt 0pt 9pt; orphans:0; widows:0"><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:36px; font-weight:bold;">LB Culture Medium Preperation</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-weight:bold; letter-spacing:-0.3pt;"> | ||
+ | </span> | ||
+ | </h1> | ||
+ | <ol type="A" style="margin:0pt; padding-left:0pt"> | ||
+ | <li | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.05pt; margin:7.05pt 0pt 0pt 24.33pt; orphans:0; padding-left:3.87pt; text-indent:0pt; widows:0"> | ||
+ | <span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">Formula</span> | ||
+ | </li> | ||
+ | </ol> | ||
+ | <p style="margin:0.3pt 0pt 0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:6pt; font-style:italic"> </span></p> | ||
+ | <table cellspacing="0" cellpadding="0" style="border-collapse:collapse; margin-left:6.5pt"> | ||
+ | <tr style="height:36pt"> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:9pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Yeast</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.7pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">extract</span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:9pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">5</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">g/L</span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style="height:36pt"> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:8.95pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Tryptone</span></p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:8.95pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">10</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">g/L</span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style="height:36pt"> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:8.9pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">NaCl</span></p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:8.9pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">10</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">g/L</span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style="height:36pt"> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:9pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Agar*</span></p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:9pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">1.5%</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">of</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">total</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">mass</span> | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style="height:36pt"> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:8.9pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">ddH</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:7.5pt">2</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">O</span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:8.95pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Desired volume</span> | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | </table> | ||
+ | <p style="margin:2.9pt 0pt 0pt 9pt; orphans:0; widows:0"><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">*Agar</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">is</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">only</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">necessary</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.05pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">when LB</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">culture</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">medium</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">is</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">prepared</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.05pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">as</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">solid</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">agar</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">gel.</span></p> | ||
+ | <ol start="2" type="A" style="margin:0pt; padding-left:0pt"> | ||
+ | <li | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.05pt; margin:7.2pt 0pt 0pt 24.33pt; orphans:0; padding-left:3.87pt; text-indent:0pt; widows:0"> | ||
+ | <span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">Sterilization</span> | ||
+ | </li> | ||
+ | </ol> | ||
+ | <p style="margin:7.2pt 0pt 0pt 51.2pt; orphans:0; widows:0"><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Sterilize</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">at</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">121</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">°C</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">for</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">30 minutes.</span></p> | ||
+ | <ol start="3" type="A" style="margin:0pt; padding-left:0pt"> | ||
+ | <li | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt; margin:7.05pt 0pt 0pt 25pt; orphans:0; padding-left:3.2pt; text-indent:0pt; widows:0"> | ||
+ | <span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">KANA</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.35pt"> | ||
+ | </span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">antibiotics</span> | ||
+ | </li> | ||
+ | </ol> | ||
+ | <p style="font-size:22px; line-height:152%; margin:7.1pt 74.45pt 0pt 51.2pt; orphans:0; widows:0"><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Concentration</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">of</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">KANA</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.7pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">antibiotics</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">in</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">solution</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">is</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.25pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">50</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">mg/μL.</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-2.85pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Functional</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">concentration</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">of</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">KANA</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.7pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">antibiotics</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">is</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">50</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">μg/μL.</span></p> | ||
+ | <p style="margin:0.2pt 0pt 0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:18pt"> </span></p> | ||
+ | <p style="margin:0.05pt 0pt 0pt 9pt; orphans:0; widows:0"><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; font-weight:bold;">E. coli Cultivation</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; font-weight:bold; letter-spacing:-0.05pt;"> | ||
+ | </span> | ||
+ | <p style="margin:7.05pt 0pt 0pt 28.2pt; orphans:0; text-indent:-19.2pt; widows:0"><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.05pt">A.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">      </span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">Protocol</span> | ||
+ | </p> | ||
+ | <p | ||
+ | style="font-size:22px; line-height:130%; margin:7.2pt 44.8pt 0pt 46.4pt; orphans:0; text-indent:-19.2pt; widows:0"> | ||
+ | <span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">1.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Clean workstation with 75% ethanol | ||
+ | and work near a lit alcohol lamp in steril</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-2.85pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">conditions.</span></p> | ||
+ | <p style="margin:3pt 0pt 0pt 46.4pt; orphans:0; text-indent:-19.25pt; widows:0"><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">2.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Prepare</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">LB</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.05pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">culture</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">medium</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">without</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">KANA</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.75pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">antibiotics.</span></p> | ||
+ | <p | ||
+ | style="font-size:22px; line-height:130%; margin:7.1pt 61.55pt 0pt 46.4pt; orphans:0; text-indent:-19.2pt; widows:0"> | ||
+ | <span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">3.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt">Prepare</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.05pt"> | ||
+ | </span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt">three</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.05pt"> | ||
+ | </span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt">lines</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px"> </span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt">of</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px"> </span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.05pt">E.</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic"> </span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.05pt">coli</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic"> </span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">with KANA</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.65pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">resistance</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.2pt"> </span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">and</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.6pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">ARSA,</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.75pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">ARSD,</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">or</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-2.85pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">ARSR</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">plasmids depending on | ||
+ | the</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">cell</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.1pt"> </span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">line.</span></p> | ||
+ | <p | ||
+ | style="font-size:22px; line-height:130%; margin:3pt 76.2pt 0pt 46.4pt; orphans:0; text-indent:-19.2pt; widows:0"> | ||
+ | <span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">4.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Add</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">KANA</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.7pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">antibiotics</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">to</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">LB</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.05pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">culture</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">medium</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">in</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">a</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">ratio</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">of 1</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">μL</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.45pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">KANA</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-2.85pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">antibiotics</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">: 1 mL</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.5pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">LB</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.15pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">culture</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.05pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">medium.</span></p> | ||
+ | <p | ||
+ | style="font-size:22px; line-height:130%; margin:3pt 66.1pt 0pt 46.4pt; orphans:0; text-indent:-19.2pt; widows:0"> | ||
+ | <span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">5.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Prepare</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">three</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">marked 15</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">mL</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.55pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">centrifure</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">tubes.</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Distribute</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">4</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">mL</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.6pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">LB</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.05pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">culture</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-2.85pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">medium</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">to</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">each tube.</span></p> | ||
+ | <p style="margin:3pt 0pt 0pt 46.4pt; orphans:0; text-indent:-19.25pt; widows:0"><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">6.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Transfer</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">10</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">μL</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.6pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">of</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">bacterial</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">solution</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">to</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.25pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">its</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">corresponding</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">tube.</span></p> | ||
+ | <p style="margin:7.2pt 0pt 0pt 46.4pt; orphans:0; text-indent:-19.25pt; widows:0"><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">7.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Cultivate</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.25pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">in a shaker</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.05pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">in</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">37 °C</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">at 220</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">rpm.</span></p> | ||
+ | </div><br style="clear:both; mso-break-type:section-break; page-break-before:always" /> | ||
+ | <div> | ||
+ | <h1 style="margin:4pt 0pt 0pt 9pt; orphans:0; widows:0"><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-weight:bold; letter-spacing:-0.05pt;">Preparation of Agar Plates</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-weight:bold;"> | ||
+ | </span> | ||
+ | </h1> | ||
+ | <p style="margin:7.05pt 0pt 0pt 28.2pt; orphans:0; text-indent:-19.2pt; widows:0"><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.05pt">A.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">      </span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">Protocol</span> | ||
+ | </p> | ||
+ | <p | ||
+ | style="font-size:22px; line-height:130%; margin:7.1pt 43.8pt 0pt 46.4pt; orphans:0; text-indent:-19.2pt; widows:0"> | ||
+ | <span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">1.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Heat the pre-prepared solid LB | ||
+ | culture medium in a microwave until the solid</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-2.85pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">completely</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">melts.</span></p> | ||
+ | <p | ||
+ | style="font-size:22px; line-height:130%; margin:3.1pt 47.25pt 0pt 46.4pt; orphans:0; text-indent:-19.2pt; widows:0"> | ||
+ | <span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">2.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Add</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">KANA</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.7pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">antibiotics</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">in</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">a</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">ratio of</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">1</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">μL</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.5pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">KANA</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.7pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">antibiotics</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">:</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">1000</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">μL</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.55pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">culture</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-2.85pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">medium.</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Mix</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">the</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">solutions | ||
+ | throughly.</span></p> | ||
+ | <p | ||
+ | style="font-size:22px; line-height:130%; margin:2.9pt 71pt 0pt 46.4pt; orphans:0; text-indent:-19.2pt; widows:0"> | ||
+ | <span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">3.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">In a</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">sterile</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">environment,</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">carefully pour</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">approximately</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">20-30 mL</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.6pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">culture</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-2.85pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">medium</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">to</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">each empty plate.</span> | ||
+ | </p> | ||
+ | <p | ||
+ | style="font-size:22px; line-height:130%; margin:3pt 42.95pt 0pt 46.4pt; orphans:0; text-indent:-19.2pt; widows:0"> | ||
+ | <span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">4.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Tilt</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">the</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">plate</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">genly to</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">cover</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">the</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">plate</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">completely</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">with</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">culture</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">medium.</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Set</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">the</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-2.85pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">plates</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">still</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">and</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.05pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">wait for</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">culture medium | ||
+ | to</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">cool</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">and</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">solidify.</span></p> | ||
+ | <p style="margin:0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:13pt"> </span></p> | ||
+ | <h1 style="margin:9.05pt 0pt 0pt 9pt; orphans:0; widows:0"><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-weight:bold;">Preservation of Transformed E. coli</span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-weight:bold; letter-spacing:-0.4pt;"> | ||
+ | </span> | ||
+ | </h1> | ||
+ | <p style="margin:7.1pt 0pt 0pt 30pt; orphans:0; text-indent:-21pt; widows:0"><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.05pt">A.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style=" font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">Materials</span> | ||
+ | </p> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div id="myModal3" class="reveal-modal"> | ||
+ | <div style="overflow:scroll;height: 700px;width:1000px"> | ||
+ | <div> | ||
+ | <h1 style="margin:4.5pt 0pt 0pt; orphans:0; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:36px; font-weight:bold; ">LB</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:36px; font-weight:bold; letter-spacing:-0.3pt; "> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:36px; font-weight:bold; ">Culture</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:36px; font-weight:bold; letter-spacing:-0.2pt; "> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:36px; font-weight:bold; ">Medium</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:36px; font-weight:bold; letter-spacing:-0.25pt; "> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:36px; font-weight:bold; ">with</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:36px; font-weight:bold; letter-spacing:-0.15pt; "> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:36px; font-weight:bold; ">Phosphate</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:36px; font-weight:bold; letter-spacing:-0.25pt; "> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:36px; font-weight:bold; ">Preparation</span> | ||
+ | </h1> | ||
+ | <p style="margin:7.1pt 0pt 0pt 28.2pt; orphans:0; text-indent:-19.2pt; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.05pt">A.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">      </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">Materials</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.2pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">(LB</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.2pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">culture</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.35pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">medium)</span> | ||
+ | </p> | ||
+ | <ol type="1" style="margin:0pt; padding-left:0pt"> | ||
+ | <li | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; margin:7.2pt 0pt 0pt 41.15pt; orphans:0; padding-left:5.25pt; text-indent:0pt; vertical-align:1pt; widows:0"> | ||
+ | <span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Refer</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">to</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">LB</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.3pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">Culture</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.35pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">Medium</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.15pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">Preparation</span> | ||
+ | </li> | ||
+ | </ol> | ||
+ | <p style="margin:7.1pt 0pt 0pt 28.2pt; orphans:0; text-indent:-19.2pt; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.05pt">B.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">      </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">Materials</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.05pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">(phosphate</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.1pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">solution)</span> | ||
+ | </p> | ||
+ | <p style="margin:0.25pt 0pt 0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:6pt; font-style:italic"> </span> | ||
+ | </p> | ||
+ | <table cellspacing="0" cellpadding="0" style="border-collapse:collapse; margin-left:6.5pt"> | ||
+ | <tr style="height:36pt"> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:8.95pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">Na</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:7.5pt">2</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">HPO</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:7.5pt">4</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">.12H</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:7.5pt">2</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">O</span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:9pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">25 | ||
+ | mg</span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style="height:36pt"> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:8.9pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">NaH</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:7.5pt">2</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">PO</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:7.5pt">4</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">.2H</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:7.5pt">2</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">O</span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:8.95pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">13 | ||
+ | mg</span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style="height:44.25pt"> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:0.4pt 0pt 0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:11pt; font-style:italic"> </span> | ||
+ | </p> | ||
+ | <p style="margin:0pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">ddH</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:7.5pt">2</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">O</span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:0.45pt 0pt 0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:11pt; font-style:italic"> </span> | ||
+ | </p> | ||
+ | <p style="margin:0pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">200 | ||
+ | mL</span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | </table> | ||
+ | <p style="margin:3pt 0pt 0pt 28.2pt; orphans:0; text-indent:-19.2pt; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.05pt">C.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">      </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">Protocol</span> | ||
+ | </p> | ||
+ | <ol type="1" style="margin:0pt; padding-left:0pt"> | ||
+ | <li | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; margin:7.05pt 0pt 0pt 41.15pt; orphans:0; padding-left:5.25pt; text-indent:0pt; vertical-align:1pt; widows:0"> | ||
+ | <span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">Weigh</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt; vertical-align:1pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">25</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt; vertical-align:1pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">mg</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt; vertical-align:1pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">Na</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:7.5pt">2</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">HPO</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:7.5pt">4</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:7.5pt; letter-spacing:0.9pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">and</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt; vertical-align:1pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">dissolve</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt; vertical-align:1pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">in</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt; vertical-align:1pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">100</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt; vertical-align:1pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">mL</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.65pt; vertical-align:1pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">ddH</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:7.5pt">2</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">O.</span> | ||
+ | </li> | ||
+ | <li | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; margin:7.05pt 0pt 0pt 41.15pt; orphans:0; padding-left:5.25pt; text-indent:0pt; vertical-align:1pt; widows:0"> | ||
+ | <span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">Weigh</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt; vertical-align:1pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">13</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt; vertical-align:1pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">mg</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt; vertical-align:1pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">NaH</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:7.5pt">2</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">PO</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:7.5pt">4</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:7.5pt; letter-spacing:0.9pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">and</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt; vertical-align:1pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">dissolve</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt; vertical-align:1pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">in</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt; vertical-align:1pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">100</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt; vertical-align:1pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">mL</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.65pt; vertical-align:1pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">ddH</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:7.5pt">2</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">O.</span> | ||
+ | </li> | ||
+ | <li | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; margin:7.2pt 0pt 0pt 41.15pt; orphans:0; padding-left:5.25pt; text-indent:0pt; vertical-align:1pt; widows:0"> | ||
+ | <span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Sterilize</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">solutions</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">at</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">121˚C</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">for</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.05pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">30</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">minutes</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">in</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">a</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">sterilization | ||
+ | pot.</span></li> | ||
+ | </ol> | ||
+ | <p style="margin:0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:13pt"> </span></p> | ||
+ | <h1 style="margin:10.85pt 0pt 0pt 9pt; orphans:0; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:36px; font-weight:bold; letter-spacing:-0.1pt; ">Function</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:36px; font-weight:bold; letter-spacing:-0.4pt; "> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:36px; font-weight:bold; letter-spacing:-0.05pt; ">Test</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:36px; font-weight:bold; letter-spacing:-0.65pt; "> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:36px; font-weight:bold; letter-spacing:-0.05pt; ">Attempt | ||
+ | 1</span></h1> | ||
+ | <p style="margin:7.1pt 0pt 0pt 28.2pt; orphans:0; text-indent:-19.2pt; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.05pt">A.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">      </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">Protocol</span> | ||
+ | </p> | ||
+ | <p style="margin:7.2pt 0pt 0pt 46.4pt; orphans:0; text-indent:-19.25pt; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">1.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Sterilize</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">workstation</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">and</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">work</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">near</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">a</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">lit</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.25pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">alcohol</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">burner.</span> | ||
+ | </p> | ||
+ | <p style="margin:7.05pt 0pt 0pt 46.4pt; orphans:0; text-indent:-19.25pt; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">2.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Prepare</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">marked | ||
+ | 15</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">mL</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.55pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">test</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">tubes</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">for</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">testing.</span></p> | ||
+ | <p | ||
+ | style="font-size:22px; line-height:130%; margin:7.2pt 57.9pt 0pt 46.4pt; orphans:0; text-indent:-19.2pt; widows:0"> | ||
+ | <span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">3.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Distribute</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">4</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">mL</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.6pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">LB</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.1pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">culture</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">medium</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">without</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">KANA</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.75pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">antibiotics</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">to</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">each | ||
+ | test</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-2.85pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">tube.</span> | ||
+ | </p> | ||
+ | <p style="margin:2.9pt 0pt 0pt 46.4pt; orphans:0; text-indent:-19.25pt; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">4.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Add</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">40</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">µL</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.55pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">E.coli | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">to</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">its</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">corresponding</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">tube.</span> | ||
+ | </p> | ||
+ | <p style="margin:7.2pt 0pt 0pt 46.4pt; orphans:0; text-indent:-19.25pt; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">5.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Cultivate</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.25pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">at</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.05pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">37˚C</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">for</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">2</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">hours</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">on | ||
+ | a</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">shake | ||
+ | bed.</span></p> | ||
+ | <p style="margin:7.05pt 0pt 0pt 46.4pt; orphans:0; text-indent:-19.25pt; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">6.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">Dilute</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt; vertical-align:1pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">NaH</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:7.5pt">2</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">PO</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:7.5pt">4</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:7.5pt; letter-spacing:0.9pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">to</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt; vertical-align:1pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">10</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt; vertical-align:1pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">mg/L.</span> | ||
+ | </p> | ||
+ | <p style="margin:7.15pt 0pt 0pt 46.4pt; orphans:0; text-indent:-19.25pt; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">7.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">Add</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt; vertical-align:1pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">NaH</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:7.5pt">2</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">PO</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:7.5pt">4</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:7.5pt; letter-spacing:1.05pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">to</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt; vertical-align:1pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">each | ||
+ | test</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt; vertical-align:1pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">tube | ||
+ | depending</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt; vertical-align:1pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">on</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt; vertical-align:1pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">the</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt; vertical-align:1pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">testing</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt; vertical-align:1pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">concentration.</span> | ||
+ | </p> | ||
+ | <p style="margin:7.05pt 0pt 0pt 28.2pt; orphans:0; text-indent:-19.2pt; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.05pt">B.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">      </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; vertical-align:1pt">NaH</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:7.5pt; font-style:italic">2</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; vertical-align:1pt">PO</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:7.5pt; font-style:italic">4</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:7.5pt; font-style:italic; letter-spacing:0.9pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; vertical-align:1pt">concentrations</span> | ||
+ | </p> | ||
+ | <p style="margin:0.4pt 0pt 0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:6pt; font-style:italic"> </span> | ||
+ | </p> | ||
+ | <table cellspacing="0" cellpadding="0" style="border-collapse:collapse; margin-left:6.5pt"> | ||
+ | <tr style="height:36pt"> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:63.5pt"> | ||
+ | <p style="margin:0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:11pt"> </span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:63.5pt"> | ||
+ | <p style="margin:8.9pt 0pt 0pt 2.9pt; orphans:0; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">0</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">µg/L</span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:63.5pt"> | ||
+ | <p style="margin:8.9pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">10</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">µg/L</span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:63.5pt"> | ||
+ | <p style="margin:8.9pt 0pt 0pt 2.9pt; orphans:0; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">20</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">µg/L</span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:63.5pt"> | ||
+ | <p style="margin:8.9pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">50</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">µg/L</span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:63.5pt"> | ||
+ | <p style="margin:8.9pt 0pt 0pt 2.9pt; orphans:0; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">100</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">µg/L</span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:63.5pt"> | ||
+ | <p style="margin:8.9pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">150</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">µg/L</span> | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style="height:56.85pt"> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:63.5pt"> | ||
+ | <p style="margin:8.85pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">NaH</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:7.5pt">2</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">PO</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:7.5pt">4</span> | ||
+ | </p> | ||
+ | <p style="margin:7.2pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">(10</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.35pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">mg/L)</span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:63.5pt"> | ||
+ | <p style="margin:0.45pt 0pt 0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:16.5pt; font-style:italic"> </span> | ||
+ | </p> | ||
+ | <p style="margin:0pt 0pt 0pt 2.9pt; orphans:0; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">0 | ||
+ | µL</span></p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:63.5pt"> | ||
+ | <p style="margin:0.45pt 0pt 0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:16.5pt; font-style:italic"> </span> | ||
+ | </p> | ||
+ | <p style="margin:0pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">4</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">µL</span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:63.5pt"> | ||
+ | <p style="margin:0.45pt 0pt 0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:16.5pt; font-style:italic"> </span> | ||
+ | </p> | ||
+ | <p style="margin:0pt 0pt 0pt 2.9pt; orphans:0; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">8 | ||
+ | µL</span></p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:63.5pt"> | ||
+ | <p style="margin:0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:11pt"> </span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:63.5pt"> | ||
+ | <p style="margin:0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:11pt"> </span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:63.5pt"> | ||
+ | <p style="margin:0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:11pt"> </span> | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style="height:36.85pt"> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:63.5pt"> | ||
+ | <p style="margin:8.95pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">NaH</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:7.5pt">2</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">PO</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:7.5pt">4</span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:63.5pt"> | ||
+ | <p style="margin:0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:11pt"> </span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:63.5pt"> | ||
+ | <p style="margin:0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:11pt"> </span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:63.5pt"> | ||
+ | <p style="margin:0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:11pt"> </span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:63.5pt"> | ||
+ | <p style="margin:9pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">2</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">µL</span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:63.5pt"> | ||
+ | <p style="margin:9pt 0pt 0pt 2.9pt; orphans:0; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">4 | ||
+ | µL</span></p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:63.5pt"> | ||
+ | <p style="margin:9pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">6</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">µL</span> | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style="height:36pt"> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:63.5pt"> | ||
+ | <p style="margin:5.95pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">(100</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">mg/L)</span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:63.5pt"> | ||
+ | <p style="margin:0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:11pt"> </span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:63.5pt"> | ||
+ | <p style="margin:0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:11pt"> </span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:63.5pt"> | ||
+ | <p style="margin:0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:11pt"> </span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:63.5pt"> | ||
+ | <p style="margin:0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:11pt"> </span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:63.5pt"> | ||
+ | <p style="margin:0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:11pt"> </span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:63.5pt"> | ||
+ | <p style="margin:0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:11pt"> </span> | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | </table> | ||
+ | </div><br style="clear:both; mso-break-type:section-break; page-break-before:auto" /> | ||
+ | <div> | ||
+ | <p style="margin:0.25pt 0pt 0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:12.5pt; font-style:italic"> </span> | ||
+ | </p> | ||
+ | <h1 style="margin:4.5pt 0pt 0pt 9pt; orphans:0; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:36px; font-weight:bold; letter-spacing:-0.1pt; ">Function</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:36px; font-weight:bold; letter-spacing:-0.4pt; "> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:36px; font-weight:bold; letter-spacing:-0.05pt; ">Test</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:36px; font-weight:bold; letter-spacing:-0.65pt; "> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:36px; font-weight:bold; letter-spacing:-0.05pt; ">Attempt | ||
+ | 2</span></h1> | ||
+ | <p style="margin:7.1pt 0pt 0pt 28.2pt; orphans:0; text-indent:-19.2pt; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.05pt">A.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">      </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">Protocol</span> | ||
+ | </p> | ||
+ | <p | ||
+ | style="font-size:22px; line-height:130%; margin:7.05pt 78.6pt 0pt 46.4pt; orphans:0; text-indent:-19.2pt; widows:0"> | ||
+ | <span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">1.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Harvest</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">3</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">mL</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.55pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">E.coli</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.05pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">bacterial</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">solution.</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Centrifuge | ||
+ | at</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">12000</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">rpm</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">for | ||
+ | 30</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-2.85pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">seconds to | ||
+ | deposit bacterial at the button of the tube. Dispose of the</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.05pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">supernatant</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">in</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">the</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.05pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">tube</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">.</span></p> | ||
+ | <p | ||
+ | style="font-size:22px; line-height:130%; margin:3.1pt 67.3pt 0pt 46.4pt; orphans:0; text-indent:-19.2pt; widows:0"> | ||
+ | <span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">2.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt">Prepare | ||
+ | new 15 mL test </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">tubes. Add LB | ||
+ | culture medium depending on the</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-2.85pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">target</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">concentration</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.1pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">of</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">phosphate.</span> | ||
+ | </p> | ||
+ | <p | ||
+ | style="font-size:22px; line-height:130%; margin:3pt 46.3pt 0pt 46.4pt; orphans:0; text-indent:-19.2pt; widows:0"> | ||
+ | <span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">3.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Transfer</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">a</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">moderate</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">amount</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">of</span><span | ||
+ | style="width:21.8pt; text-indent:0pt; display:inline-block; -aw-tabstop-align:left; -aw-tabstop-pos:206.6pt"></span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">LB culture medium | ||
+ | from th</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">e test tube to | ||
+ | the</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-2.85pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">centrifuge | ||
+ | tube.</span></p> | ||
+ | <p | ||
+ | style="font-size:22px; line-height:130%; margin:3pt 64.8pt 0pt 46.4pt; orphans:0; text-indent:-19.2pt; widows:0"> | ||
+ | <span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">4.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Levitate</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">the</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">bacteria</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">in</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">the</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">centrifuge</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">tube.</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.3pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Transfer</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">the</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">bacterial</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">solution</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-2.85pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">back</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">to</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">test | ||
+ | tube.</span></p> | ||
+ | <p style="margin:3pt 0pt 0pt 46.4pt; orphans:0; text-indent:-19.25pt; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">5.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Add</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">phosphate | ||
+ | solution</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">to</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">the</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">test</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">tube,</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">depending</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">on</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">the | ||
+ | concentration.</span></p> | ||
+ | <p style="margin:7.05pt 0pt 0pt 46.4pt; orphans:0; text-indent:-19.25pt; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">6.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Cultivate</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.25pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">at</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.05pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">37˚C</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">and | ||
+ | 220</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">rpm</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">for 2 | ||
+ | hours.</span></p> | ||
+ | <p style="margin:7.25pt 0pt 0pt 28.2pt; orphans:0; text-indent:-19.2pt; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.05pt">B.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">      </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">Solution</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.2pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">amounts</span> | ||
+ | </p> | ||
+ | <p style="margin:0.2pt 0pt 0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:6pt; font-style:italic"> </span> | ||
+ | </p> | ||
+ | <table cellspacing="0" cellpadding="0" style="border-collapse:collapse; margin-left:6.5pt"> | ||
+ | <tr style="height:36pt"> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:75.35pt"> | ||
+ | <p style="margin:0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:11pt"> </span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:51.65pt"> | ||
+ | <p style="margin:8.9pt 0pt 0pt 2.9pt; orphans:0; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">0</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">mg/L</span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:63.5pt"> | ||
+ | <p style="margin:8.9pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">1</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">mg/L</span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:63.5pt"> | ||
+ | <p style="margin:8.9pt 0pt 0pt 2.9pt; orphans:0; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">10</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">mg/L</span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:63.5pt"> | ||
+ | <p style="margin:8.9pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">20</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">mg/L</span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:63.5pt"> | ||
+ | <p style="margin:8.9pt 0pt 0pt 2.9pt; orphans:0; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">50</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">mg/L</span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:63.5pt"> | ||
+ | <p style="margin:8.9pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">100</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">mg/L</span> | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style="height:56.8pt"> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:75.35pt"> | ||
+ | <p style="margin:8.8pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">NaH</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:7.5pt">2</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">PO</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:7.5pt">4</span> | ||
+ | </p> | ||
+ | <p style="margin:7.25pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">(100</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">mg/L)</span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:51.65pt"> | ||
+ | <p style="margin:0.45pt 0pt 0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:16.5pt; font-style:italic"> </span> | ||
+ | </p> | ||
+ | <p style="margin:0pt 0pt 0pt 2.9pt; orphans:0; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">0 | ||
+ | µL</span></p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:63.5pt"> | ||
+ | <p style="margin:0.45pt 0pt 0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:16.5pt; font-style:italic"> </span> | ||
+ | </p> | ||
+ | <p style="margin:0pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">40</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">µL</span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:63.5pt"> | ||
+ | <p style="margin:0.45pt 0pt 0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:16.5pt; font-style:italic"> </span> | ||
+ | </p> | ||
+ | <p style="margin:0pt 0pt 0pt 2.9pt; orphans:0; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">400 | ||
+ | µL</span></p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:63.5pt"> | ||
+ | <p style="margin:0.45pt 0pt 0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:16.5pt; font-style:italic"> </span> | ||
+ | </p> | ||
+ | <p style="margin:0pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">800</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">µL</span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:63.5pt"> | ||
+ | <p style="margin:0.45pt 0pt 0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:16.5pt; font-style:italic"> </span> | ||
+ | </p> | ||
+ | <p style="margin:0pt 0pt 0pt 2.9pt; orphans:0; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">2000 | ||
+ | µL</span></p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:63.5pt"> | ||
+ | <p style="margin:0.45pt 0pt 0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:16.5pt; font-style:italic"> </span> | ||
+ | </p> | ||
+ | <p style="margin:0pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">4000</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">µL</span> | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style="height:53.9pt"> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:75.35pt"> | ||
+ | <p | ||
+ | style="font-size:22px; line-height:130%; margin:8.95pt 20.5pt 0pt 2.95pt; orphans:0; widows:0"> | ||
+ | <span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">LB</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.75pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">culture</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-2.85pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">medium</span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:51.65pt"> | ||
+ | <p style="margin:0.15pt 0pt 0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:15.5pt; font-style:italic"> </span> | ||
+ | </p> | ||
+ | <p style="margin:0pt 0pt 0pt 2.9pt; orphans:0; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">4000 | ||
+ | µL</span></p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:63.5pt"> | ||
+ | <p style="margin:0.15pt 0pt 0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:15.5pt; font-style:italic"> </span> | ||
+ | </p> | ||
+ | <p style="margin:0pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">3960</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">µL</span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:63.5pt"> | ||
+ | <p style="margin:0.15pt 0pt 0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:15.5pt; font-style:italic"> </span> | ||
+ | </p> | ||
+ | <p style="margin:0pt 0pt 0pt 2.9pt; orphans:0; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">3600 | ||
+ | µL</span></p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:63.5pt"> | ||
+ | <p style="margin:0.15pt 0pt 0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:15.5pt; font-style:italic"> </span> | ||
+ | </p> | ||
+ | <p style="margin:0pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">3200</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">µL</span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:63.5pt"> | ||
+ | <p style="margin:0.15pt 0pt 0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:15.5pt; font-style:italic"> </span> | ||
+ | </p> | ||
+ | <p style="margin:0pt 0pt 0pt 2.9pt; orphans:0; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">2000 | ||
+ | µL</span></p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:63.5pt"> | ||
+ | <p style="margin:0.15pt 0pt 0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:15.5pt; font-style:italic"> </span> | ||
+ | </p> | ||
+ | <p style="margin:0pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">0</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">µL</span> | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | </table> | ||
+ | <p style="margin:0.15pt 0pt 0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:16pt; font-style:italic"> </span> | ||
+ | </p> | ||
+ | <h1 style="margin:0pt 0pt 0pt 9pt; orphans:0; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:36px; font-weight:bold; letter-spacing:-0.1pt; ">Function</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:36px; font-weight:bold; letter-spacing:-0.4pt; "> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:36px; font-weight:bold; letter-spacing:-0.05pt; ">Test</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:36px; font-weight:bold; letter-spacing:-0.65pt; "> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:36px; font-weight:bold; letter-spacing:-0.05pt; ">Attempt | ||
+ | 3</span></h1> | ||
+ | <p style="margin:7.2pt 0pt 0pt 28.2pt; orphans:0; text-indent:-19.2pt; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.05pt">A.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">      </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">Protocol</span> | ||
+ | </p> | ||
+ | <p style="margin:7.1pt 0pt 0pt 30pt; orphans:0; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Refer</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.25pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">to</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.25pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">Function</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.2pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">Test</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.6pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">Attempt</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.25pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">2</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">.</span></p> | ||
+ | <p style="margin:7.2pt 0pt 0pt 28.2pt; orphans:0; text-indent:-19.2pt; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.05pt">B.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">      </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">Notes</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">:</span></p> | ||
+ | <p style="margin:7pt 0pt 0pt 30pt; orphans:0; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">C</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:7.5pt">2</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">H</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:7.5pt">12</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">AsNaO</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:7.5pt">2</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:7.5pt; letter-spacing:0.9pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">is</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt; vertical-align:1pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">used</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt; vertical-align:1pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">instead</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt; vertical-align:1pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">of</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt; vertical-align:1pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">NaH</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:7.5pt">2</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">PO</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:7.5pt">4</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">.</span> | ||
+ | </p> | ||
+ | <p style="margin:7.25pt 0pt 0pt 28.2pt; orphans:0; text-indent:-19.2pt; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.05pt">C.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">      </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">C2H12AsNaO2</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.25pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">amounts</span> | ||
+ | </p> | ||
+ | <p style="margin:7pt 0pt 0pt 30pt; orphans:0; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">Same</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.25pt; vertical-align:1pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">as</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt; vertical-align:1pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">the</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt; vertical-align:1pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">amounts</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt; vertical-align:1pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">of</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt; vertical-align:1pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">NaH</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:7.5pt">2</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">PO</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:7.5pt">4</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:7.5pt; letter-spacing:0.9pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">in</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt; vertical-align:1pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; vertical-align:1pt">Function</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.2pt; vertical-align:1pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; vertical-align:1pt">Test</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.4pt; vertical-align:1pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; vertical-align:1pt">Attempt</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.15pt; vertical-align:1pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; vertical-align:1pt">2</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">.</span> | ||
+ | </p> | ||
+ | <p style="margin:0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:13pt"> </span></p> | ||
+ | <p style="margin:0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:11.5pt"> </span></p> | ||
+ | <p style="margin:0pt 0pt 0pt 9pt; orphans:0; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:36px; font-style:italic; font-weight:bold">To</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:36px; font-style:italic; font-weight:bold; letter-spacing:-0.65pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:36px; font-style:italic; font-weight:bold">be</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:36px; font-style:italic; font-weight:bold; letter-spacing:-0.5pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:36px; font-style:italic; font-weight:bold">continued....</span> | ||
+ | </p> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | <!--bottom--> | ||
+ | <div style="background-color: #F9F9F9;"> | ||
+ | <div style="width: 80%;height: 1080px;margin: 0 auto;padding: 10% 0;"> | ||
+ | <span | ||
+ | style="font-size: 48px;display: block;padding-bottom: 70px;">CONTACT INFO</span> | ||
+ | <span | ||
+ | style="text-align: center;line-height: 90px;letter-spacing: 2px;font-size: 36px;display: block;font-family: -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen, Ubuntu, Cantarell, 'Open Sans', 'Helvetica Neue', sans-serif;"> | ||
+ | Please scan the following QR codes to find more about us on | ||
+ | </span> | ||
+ | <span | ||
+ | style="text-align: center;font-weight: bold;line-height: 40px;letter-spacing: 2px;font-size: 36px;display: block;font-family: -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen, Ubuntu, Cantarell, 'Open Sans', 'Helvetica Neue', sans-serif;"> | ||
+ | Weibo、Bilibili、Wechat ! | ||
+ | </span> | ||
+ | <img style="width:810px;height: 345px;margin: 100px auto;display: block;" src="https://static.igem.org/mediawiki/2021/7/71/T--Shanghai_United--homepage07.jpg"> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | </body> | ||
+ | |||
+ | <script> | ||
+ | |||
+ | (function($) { | ||
+ | |||
+ | /*--------------------------- | ||
+ | Defaults for Reveal | ||
+ | ----------------------------*/ | ||
+ | |||
+ | /*--------------------------- | ||
+ | Listener for data-reveal-id attributes | ||
+ | ----------------------------*/ | ||
+ | |||
+ | $('a[data-reveal-id]').live('click', function(e) { | ||
+ | e.preventDefault(); | ||
+ | var modalLocation = $(this).attr('data-reveal-id'); | ||
+ | $('#'+modalLocation).reveal($(this).data()); | ||
+ | }); | ||
+ | |||
+ | /*--------------------------- | ||
+ | Extend and Execute | ||
+ | ----------------------------*/ | ||
+ | |||
+ | $.fn.reveal = function(options) { | ||
+ | |||
+ | |||
+ | var defaults = { | ||
+ | animation: 'fadeAndPop', //fade, fadeAndPop, none | ||
+ | animationspeed: 300, //how fast animtions are | ||
+ | closeonbackgroundclick: true, //if you click background will modal close? | ||
+ | dismissmodalclass: 'close-reveal-modal' //the class of a button or element that will close an open modal | ||
+ | }; | ||
+ | |||
+ | //Extend dem' options | ||
+ | var options = $.extend({}, defaults, options); | ||
+ | |||
+ | return this.each(function() { | ||
+ | |||
+ | /*--------------------------- | ||
+ | Global Variables | ||
+ | ----------------------------*/ | ||
+ | var modal = $(this), | ||
+ | topMeasure = parseInt(modal.css('top')), | ||
+ | topOffset = modal.height() + topMeasure, | ||
+ | locked = false, | ||
+ | modalBG = $('.reveal-modal-bg'); | ||
+ | |||
+ | /*--------------------------- | ||
+ | Create Modal BG | ||
+ | ----------------------------*/ | ||
+ | if(modalBG.length == 0) { | ||
+ | modalBG = $('<div class="reveal-modal-bg" />').insertAfter(modal); | ||
+ | } | ||
+ | |||
+ | /*--------------------------- | ||
+ | Open & Close Animations | ||
+ | ----------------------------*/ | ||
+ | //Entrance Animations | ||
+ | modal.bind('reveal:open', function () { | ||
+ | modalBG.unbind('click.modalEvent'); | ||
+ | $('.' + options.dismissmodalclass).unbind('click.modalEvent'); | ||
+ | if(!locked) { | ||
+ | lockModal(); | ||
+ | if(options.animation == "fadeAndPop") { | ||
+ | modal.css({'top': $(document).scrollTop()-topOffset, 'opacity' : 0, 'visibility' : 'visible'}); | ||
+ | modalBG.fadeIn(options.animationspeed/2); | ||
+ | modal.delay(options.animationspeed/2).animate({ | ||
+ | "top": $(document).scrollTop()+topMeasure + 'px', | ||
+ | "opacity" : 1 | ||
+ | }, options.animationspeed,unlockModal()); | ||
+ | } | ||
+ | if(options.animation == "fade") { | ||
+ | modal.css({'opacity' : 0, 'visibility' : 'visible', 'top': $(document).scrollTop()+topMeasure}); | ||
+ | modalBG.fadeIn(options.animationspeed/2); | ||
+ | modal.delay(options.animationspeed/2).animate({ | ||
+ | "opacity" : 1 | ||
+ | }, options.animationspeed,unlockModal()); | ||
+ | } | ||
+ | if(options.animation == "none") { | ||
+ | modal.css({'visibility' : 'visible', 'top':$(document).scrollTop()+topMeasure}); | ||
+ | modalBG.css({"display":"block"}); | ||
+ | unlockModal() | ||
+ | } | ||
+ | } | ||
+ | modal.unbind('reveal:open'); | ||
+ | }); | ||
+ | |||
+ | //Closing Animation | ||
+ | modal.bind('reveal:close', function () { | ||
+ | if(!locked) { | ||
+ | lockModal(); | ||
+ | if(options.animation == "fadeAndPop") { | ||
+ | modalBG.delay(options.animationspeed).fadeOut(options.animationspeed); | ||
+ | modal.animate({ | ||
+ | "top": $(document).scrollTop()-topOffset + 'px', | ||
+ | "opacity" : 0 | ||
+ | }, options.animationspeed/2, function() { | ||
+ | modal.css({'top':topMeasure, 'opacity' : 1, 'visibility' : 'hidden'}); | ||
+ | unlockModal(); | ||
+ | }); | ||
+ | } | ||
+ | if(options.animation == "fade") { | ||
+ | modalBG.delay(options.animationspeed).fadeOut(options.animationspeed); | ||
+ | modal.animate({ | ||
+ | "opacity" : 0 | ||
+ | }, options.animationspeed, function() { | ||
+ | modal.css({'opacity' : 1, 'visibility' : 'hidden', 'top' : topMeasure}); | ||
+ | unlockModal(); | ||
+ | }); | ||
+ | } | ||
+ | if(options.animation == "none") { | ||
+ | modal.css({'visibility' : 'hidden', 'top' : topMeasure}); | ||
+ | modalBG.css({'display' : 'none'}); | ||
+ | } | ||
+ | } | ||
+ | modal.unbind('reveal:close'); | ||
+ | }); | ||
+ | |||
+ | /*--------------------------- | ||
+ | Open and add Closing Listeners | ||
+ | ----------------------------*/ | ||
+ | //Open Modal Immediately | ||
+ | modal.trigger('reveal:open') | ||
+ | |||
+ | //Close Modal Listeners | ||
+ | var closeButton = $('.' + options.dismissmodalclass).bind('click.modalEvent', function () { | ||
+ | modal.trigger('reveal:close') | ||
+ | }); | ||
+ | |||
+ | if(options.closeonbackgroundclick) { | ||
+ | modalBG.css({"cursor":"pointer"}) | ||
+ | modalBG.bind('click.modalEvent', function () { | ||
+ | modal.trigger('reveal:close') | ||
+ | }); | ||
+ | } | ||
+ | $('body').keyup(function(e) { | ||
+ | if(e.which===27){ modal.trigger('reveal:close'); } // 27 is the keycode for the Escape key | ||
+ | }); | ||
+ | |||
+ | |||
+ | /*--------------------------- | ||
+ | Animations Locks | ||
+ | ----------------------------*/ | ||
+ | function unlockModal() { | ||
+ | locked = false; | ||
+ | } | ||
+ | function lockModal() { | ||
+ | locked = true; | ||
+ | } | ||
+ | |||
+ | });//each call | ||
+ | }//orbit plugin call | ||
+ | })(jQuery); | ||
+ | |||
+ | //由于模块都一次性加载,因此不用执行 layui.use() 来加载对应模块,直接使用即可: | ||
+ | ; | ||
+ | ! function () { | ||
+ | var layer = layui.layer, | ||
+ | form = layui.form; | ||
+ | }(); | ||
+ | layui.use('carousel', function () { | ||
+ | var carousel = layui.carousel; | ||
+ | //建造实例 | ||
+ | carousel.render({ | ||
+ | elem: '#test1' | ||
+ | , width: '100%' //设置容器宽度 | ||
+ | , arrow: 'always' //始终显示箭头 | ||
+ | //,anim: 'updown' //切换动画方式 | ||
+ | }); | ||
+ | }); | ||
+ | </script> | ||
+ | <script src="https://2021.igem.org/Team:Shanghai_United/Template:JS?action=raw&ctype=text/javascript"></script> | ||
</html> | </html> |
Latest revision as of 05:25, 21 October 2021
![](https://static.igem.org/mediawiki/2021/2/27/T--Shanghai_United--homepage01.png)
![](https://static.igem.org/mediawiki/2021/9/95/T--Shanghai_United--homepage08.jpg)
Experiments
PCR of the target Gene
A. Materials
ddH2O |
32 μL |
dNTP |
5 μL |
MgSO4 |
4 μL |
Forward primer |
1 μL |
Reverse primer |
1 μL |
DNA template |
1 μL |
KOD+ buffer |
5 μL |
KOD+ |
1 μL |
B. Protocol
1. Prepare and mark PCR tubes and align tubes in order on tube rack.
2. Under normal circumstances, ddH2O should be added into tubes first, and enzymes should be added last. All other materials uncluding, but are not limited to dNTP, primers, buffers, and templates should be added in between.
3. PCR tubes should then be centrifuged briefly to ensure all liquids collect at the bottom of the tube.
4. Move PCR tubes to PCR machine to initiate the process.
C. Reacting conditions
98 °C |
10 minutes |
1 cycle |
98 °C |
15 seconds |
26 cycles |
55 °C |
15 seconds |
|
68 °C |
1 minute |
|
68 °C |
10 minutes |
1 cycle |
4 °C |
Infinite |
N/A |
Gel Electrophoresis of the Gene PCR Product A. Materials
Loading buffer & DNA dye |
1 μL |
PCR product |
5 μL |
TAE buffer |
200 mL |
Agarose |
2 g |
B. Protocol
1. Mix TAE buffer and agarose powder in a ratio of 100 mL TAE buffer : 1 g agarose powder.
2. Heat the mixed solution in a microwave until agarose powder completely dissolves into TAE buffer.
3. Pour the liquid into gel trays of suitable sizes. Insert a comb onto the tray to
4. Mix 6x loading buffer premixed with DNA dye with PCR product in a ratio of 1 unit loading buffer : 5 units PCR product.
5. Load proper ladder into first well of gel.
6. Load samples into wells.
7. Perform electrophoresis at 185 V for 10 minutes.
8. Harvest gel, take image of gel under untraviolet light, and analyze results.
PCR Product Clean Up
A. Protocol (MN NucleoSpin Gel and PCR Clean-up Kit)
1. Combine obtained PCR products into one centrifuge tube.
2. Add buffer NTI to the product in a ratio of 2 μL buffer NTI : 1 μL PCR product to allow DNA to bind to the membrane.
3. Transfer the solution to a column placed within a collection tube. Centrifuge
at 12000 rpm for 30 seconds.
4. Transfer the liquid in the collection tube to the column. Centrifuge at 12000 rpm for 30 seconds.
5. Dispose of the liquid in the collection tube. Add 700 μL buffer NT3 to wash the membrane. Centrifuge at 12000 rpm for 30 seconds.
6. Repeat step 5.
7. Add 30 μL ddH2O preheated to 65 ˚C to the column. Centrifuge at 12000 rpm for 30 seconds.
B. Buffers
Buffer NTI: Adjust DNA binding conditions and allow DNA to bind to membrane.
Buffer NT3: Wash membrane.
Plasmid Extraction
A. Protocol (TIAN Prep Mini Plasmid Kit)
1. Harvest and transfer cultivated bacteria into marked 2 mL centrifuge tubes.
2. Centrifuge tubes at 12000 rpm for 30 seconds to deposit bacteria to the bottom of the tube. Dispose of the clear liquid on top.
3. Add 250 μL buffer P1 into the 2 mL centrifuge tubes to levitete the bacteria persent in the tubes.
4. Add 250 μL buffer P2 to the tubes to lyse bacterial cell wall. Mix the solution gently; do not vortex.
5. Add 350 μL buffer P3 to the tubes to neutralize buffer P2 and cause proteins in the solution to percipitate. Mix the solution gently; do not vortex.
6. Centrifuge the solution at 12000 rpm for 10 minutes to collect percitipation to the bottom of the tube.
7. Add 500 μL buffer BL to a column placed within a collection tube to wash the membrane. Centrifuge at 12000 rpm for 30 seconds.
8. Transfer the clear liquid obtained in step 6 to the column washed in step 7 and centrifuge the column at 12000 rpm for 30 seconds.
9. Dispose the clear liquid in the collection tube. Add 500 μL buffer PD to the column and centrifuge at 12000 rpm for 30 seconds.
10. Dispose of the liquid in the collection tube. Add 600 μL buffer PW to the column. Centrifuge at 12000 rpm for 30 seconds.
11. Repeat step 10.
12. Dispose of the clear liquid in the collection tube. Centrifuge at 12000 rpm for 1 minute.
13. Transfer the column to a new 2 mL centrifiuge tube. Dispose of the collection tube.
14. Add 40 μL ddH2O preheated to 65 °C to the column. Centrifuge at 12000 rpm for 30 seconds.
B. Buffers
Buffer P1: Levitate bacteria and lyse RNA. Buffer P2: Lyse bacteria cell wall.
Buffer P3: Neutralize P2 and cause proteins to precipitate. Buffer BL: Wash memnbrane pre-use.
Buffer PD: Wash proteins off the membrane. Buffer PW: Wash salts off the membrane.
Restriction Enzyme Digestion
A. Materials
ARSA/ARSR/ARSD |
|
10x NEB buffer |
5 μL |
ARSA/D/R Plasmid (300 ng/μL) |
10 μL |
BsaI restriction enzyme |
1 μL |
ddH2O |
34 μL |
amilGFP |
|
10x NEB buffer |
5 μL |
amilGFP (400 ng/μL) |
10 μL |
BsaI |
1 μL |
DpnI |
1 μL |
ddH2O |
33 μL |
B. Protocol
1. Mix the materials in a 1.5 mL centrifuge tube.
2. Place the centrifuge tube in 37 °C metal bath for at least 15 minutes.
Gel Electrophoresis of Restriction Enzyme Digestion Product
A. Materials
Loading buffer with DNA dye |
10 μL |
Digestion product |
50 μL |
B. Protocol
1. Mix 6x loading buffer premixed with DNA dye with PCR product in a ratio of 1 unit loading buffer : 5 units PCR product.
2. Load proper ladder into first well of gel.
3. Load samples into wells.
4. Perform electrophoresis at 185 V for 10 minutes.
5. Harvest gel, take image of gel under untraviolet light, and analyze results.
Agarose Gel DNA Extraction
A. Protocol (MN NucleoSpin Gel and PCR Clean-up Kit)
1. Collect gel with desired DNA fragments into 2 mL centrifuge tubes. Weigh the mass of gels on an electronic balance.
2. Add buffer NTI to the tube in a ratio of 2 μL buffer NTI : 1 mg agarose gel.
3. Heat the mixture at 50 ˚C in a metal bath until the gel dissolves completely.
4. Transfer the solution to a new column placed within a collection tube. Centrifuge at 12000 rpm for 30 seconds.
5. Transfer the liquid in the collection tube to the column. Centrifuge at 12000 rpm for 30 seconds.
6. Dispose of the clear liquid in the collection tube. Add 700 μL buffer NT3 to
the column. Centrifuge at 12000 rpm for 30 seconds.
7. Repeat step 6.
8. Dispose of the liquid in the collection tube. Centrifuge at 12000 rpm for 1 minute.
9. Add 30 μL ddH2O preheated to 65 ˚C to the column. Centrifuge at 12000 rpm for 30 seconds.
T4 DNA Ligase Ligation
A. Materials
ARSA/R/D plasmid (30 ng/μL) |
1.5 μL |
GFP (100 ng/μL) |
4.5 μL |
T4 DNA ligase |
1 μL |
T4 DNA ligase buffer |
1 μL |
ddH2O |
2 μL |
B. Protocol
1. Add ddH2O, T4 DNA ligase buffer, and T4 DNA ligase into a PCR tube with GFP gene and ARS plasmid in a ratio of 1 units ARS plasmid : 10 unit GFP gene.
2. Transfer the solution to PCR machine at 16˚C.
Transformation of DH5-α E. coli Using Heat Shock
A. Protocol
1. Mix the tube introduced DNA material with DH5-α competent cells. The volumn of DNA material must not exceed 10% of that of the competent cells.
2. Gently fick the tube 4-5 times to mix competent cells and DNA material. Do not vortex.
3. Place the tube on ice for 30 minutes.
4. Place the tube at exactly 42 ˚C for 90 seconds to perform heat shock.
5. Immediately place the tube back on ice after heat shock. Keep the tube on ice for 2-3 minutes.
6. Add 900 μL LB culture medium without KANA antibiotics to the tube. Incubate the transformed bacteria at 37 ˚C for 30 minutes.
7. Centrifuge the tube at 12000 rpm for 30 seconds.
8. Dispose half of the clear liquid in the centrifuge tube. Mix and levitate the bacteria on the bottom of the tube with a micropipette.
9. In a sterile environment, transfer the transformed bacteria to prepared agar plates.
Colony PCR Identification
A. Materials
2x PCR mix |
10 μL |
Forward primer |
0.5 μL |
Reverse primer |
0.5 μL |
Template |
Desired E. Coli |
ddH2O |
9 μL |
B. Protocol
1. Refer to PCR of amilGFP Gene, Jul. 28, 2021.
2. Notes to this reaction:
a. Primers were delivered in the form of dry powder. Centrifuge primers at 12000 rpm for 5 minutes to deposit powder at the bottom of its tube.
b. Dilute primers to a concentration of 10 nmol/ml.
c. Mix the 2x PCR mix, R.primer, Templalate, ddH2O as master mix.
C. Reacting conditions
98 °C |
10 minutes |
1 cycle |
98 °C |
15 seconds |
26 cycles |
55 °C |
15 seconds |
|
72 °C |
30 seconds |
|
72 °C |
5 minutes |
1 cycle |
4 °C |
Infinite |
N/A |
LB Culture Medium Preperation
- Formula
Yeast extract |
5 g/L |
Tryptone |
10 g/L |
NaCl |
10 g/L |
Agar* |
1.5% of total mass |
ddH2O |
Desired volume |
*Agar is only necessary when LB culture medium is prepared as solid agar gel.
- Sterilization
Sterilize at 121 °C for 30 minutes.
- KANA antibiotics
Concentration of KANA antibiotics in solution is 50 mg/μL. Functional concentration of KANA antibiotics is 50 μg/μL.
E. coli Cultivation
A. Protocol
1. Clean workstation with 75% ethanol and work near a lit alcohol lamp in steril conditions.
2. Prepare LB culture medium without KANA antibiotics.
3. Prepare three lines of E. coli with KANA resistance and ARSA, ARSD, or ARSR plasmids depending on the cell line.
4. Add KANA antibiotics to LB culture medium in a ratio of 1 μL KANA antibiotics : 1 mL LB culture medium.
5. Prepare three marked 15 mL centrifure tubes. Distribute 4 mL LB culture medium to each tube.
6. Transfer 10 μL of bacterial solution to its corresponding tube.
7. Cultivate in a shaker in 37 °C at 220 rpm.
Preparation of Agar Plates
A. Protocol
1. Heat the pre-prepared solid LB culture medium in a microwave until the solid completely melts.
2. Add KANA antibiotics in a ratio of 1 μL KANA antibiotics : 1000 μL culture medium. Mix the solutions throughly.
3. In a sterile environment, carefully pour approximately 20-30 mL culture medium to each empty plate.
4. Tilt the plate genly to cover the plate completely with culture medium. Set the plates still and wait for culture medium to cool and solidify.
Preservation of Transformed E. coli
A. Materials
LB Culture Medium with Phosphate Preparation
A. Materials (LB culture medium)
- Refer to LB Culture Medium Preparation
B. Materials (phosphate solution)
Na2HPO4.12H2O |
25 mg |
NaH2PO4.2H2O |
13 mg |
ddH2O |
200 mL |
C. Protocol
- Weigh 25 mg Na2HPO4 and dissolve in 100 mL ddH2O.
- Weigh 13 mg NaH2PO4 and dissolve in 100 mL ddH2O.
- Sterilize solutions at 121˚C for 30 minutes in a sterilization pot.
Function Test Attempt 1
A. Protocol
1. Sterilize workstation and work near a lit alcohol burner.
2. Prepare marked 15 mL test tubes for testing.
3. Distribute 4 mL LB culture medium without KANA antibiotics to each test tube.
4. Add 40 µL E.coli to its corresponding tube.
5. Cultivate at 37˚C for 2 hours on a shake bed.
6. Dilute NaH2PO4 to 10 mg/L.
7. Add NaH2PO4 to each test tube depending on the testing concentration.
B. NaH2PO4 concentrations
|
0 µg/L |
10 µg/L |
20 µg/L |
50 µg/L |
100 µg/L |
150 µg/L |
NaH2PO4 (10 mg/L) |
0 µL |
4 µL |
8 µL |
|
|
|
NaH2PO4 |
|
|
|
2 µL |
4 µL |
6 µL |
(100 mg/L) |
|
|
|
|
|
|
Function Test Attempt 2
A. Protocol
1. Harvest 3 mL E.coli bacterial solution. Centrifuge at 12000 rpm for 30 seconds to deposit bacterial at the button of the tube. Dispose of the supernatant in the tube .
2. Prepare new 15 mL test tubes. Add LB culture medium depending on the target concentration of phosphate.
3. Transfer a moderate amount ofLB culture medium from the test tube to the centrifuge tube.
4. Levitate the bacteria in the centrifuge tube. Transfer the bacterial solution back to test tube.
5. Add phosphate solution to the test tube, depending on the concentration.
6. Cultivate at 37˚C and 220 rpm for 2 hours.
B. Solution amounts
|
0 mg/L |
1 mg/L |
10 mg/L |
20 mg/L |
50 mg/L |
100 mg/L |
NaH2PO4 (100 mg/L) |
0 µL |
40 µL |
400 µL |
800 µL |
2000 µL |
4000 µL |
LB culture medium |
4000 µL |
3960 µL |
3600 µL |
3200 µL |
2000 µL |
0 µL |
Function Test Attempt 3
A. Protocol
Refer to Function Test Attempt 2.
B. Notes:
C2H12AsNaO2 is used instead of NaH2PO4.
C. C2H12AsNaO2 amounts
Same as the amounts of NaH2PO4 in Function Test Attempt 2.
To be continued....
![](https://static.igem.org/mediawiki/2021/7/71/T--Shanghai_United--homepage07.jpg)