Difference between revisions of "Team:Shanghai HS ID/Safety"

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<h1> Safety </h1>
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<p>Please visit the <a href="https://2021.igem.org/Safety">Safety Hub</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
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    <title>Shanghai_HS_ID</title>
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<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
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<h3>Safe Project Design</h3>
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<p>Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:</p>
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<ul>
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<li>Choosing a non-pathogenic chassis</li>
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<li>Choosing parts that will not harm humans / animals / plants</li>
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<li>Substituting safer materials for dangerous materials in a proof-of-concept experiment</li>
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<li>Including an "induced lethality" or "kill-switch" device</li>
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<h3>Safe Lab Work</h3>
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<p>What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!</p>
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                    <a href="https://2021.igem.org/Team:Shanghai_HS_ID">Home</a>
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                                    Collection</a></li>
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                    <a href="https://2021.igem.org/Team:Shanghai_HS_ID/Model">Model</a>
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    <div class="sub-content">
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        <div class="sub-title">Safety</div>
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        <div class="article-title">General Lab Safety</div>
 +
        <div class="article-content">Our lab at ATLATL has the physical protection level of BSL-1, which means we only
 +
            deal with microorganisms with no pathogenicity to healthy adults. </div>
 +
        <div class="article-title">Fire Safety</div>
 +
        <div class="article-content">There are fire hydrants, fire extinguishers, automatic spray system, manual fire
 +
            alarm, fireproof shutter doors, and smoke alarms in the laboratory area. And the escaping route is
 +
            well-managed. So our lab meets the national fire safety regulations. </div>
 +
        <div class="article-title">Individual Protection</div>
 +
        <div class="article-content">Eating or drinking is not allowed in the lab area. Besides, white coats and PVC
 +
            gloves are required and only in the lab. Goggles are needed for hazardous experiments and an eye washer is
 +
            provided for unexpected accidents. </div>
 +
        <div class="article-title">Emergency Handling</div>
 +
        <div class="article-content">Once the laboratory encounters a dangerous or unmanageable situation (such as
 +
            experimental accidents, fires, leakage of dangerous goods, etc.), the person in charge of the laboratory
 +
            will arrange for the relevant team to deal with it immediately. This laboratory is equipped with trained
 +
            personnel to ensure a rapid response for any emergency.</div>
 +
        <div class="article-title">Project Safety</div>
 +
        <div class="article-content">Microorganism </div>
 +
        <div class="article-content">We only used E. coli DH5α and Lactobacillus casei which are harmless to human
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            beings and all related experiments were conducted under the supervision of our instructor, Ms. Song. </div>
 +
        <div class="article-content">The plasmids we used in the project are listed below:</div>
 +
        <div class="article-content" style="padding-left: 30px; box-sizing: border-box;">
 +
            <b>pLCNICK(Addgene, #84653) <br />
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                pIB165(Addgene, #90188)</b>
 +
        </div>
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        <div class="article-content">The primers we used in the project are listed below:</div>
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        <div class="article-content" style="padding-left: 30px; box-sizing: border-box;">
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            <b>• Apal-334-2094-down-F：</b><br />
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            CTAAGGATCACATCTTTTTCTAAACTAGGGCCCAACCAATCGTATCTTTGACAA<br />
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            <b>• BSAI-334 2094-down-R：</b><br />
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            GATTAAACCTCCTTAAATTGCCCAGA GTACTCATTCC<br />
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            <b>• BSAI-334 2094-up-F：</b><br />
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            TTTGGAGGAATTTAACGGGTCTCAGAGTAAGGATGCTAAAAAC<br />
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            <b>• BSAI-334 2094-up-R：</b><br />
 +
            TTTTTTTGAGGAGACCCATGTTGTCTAGCTCAAGCA<br />
 +
            <b>• BSAI-334 2094-sgRNA-F：</b><br />
 +
            TTGAGCTAGACAACATGGGTCTCCTCAAAAAAAGCACCGACT<br />
 +
            <b>• BSAI-334 2094-sgRNA-R1：</b><br />
 +
            ACGATAAAGATCGAGATTTTGGCAATACAATACAACTATGCTGTC<br />
 +
            <b>• BSAI-334 2094-sgRNA-R2：</b><br />
 +
            ATGCTGTCTAGATCTCCACTATAGTAGGAAAGAATACACG<br />
 +
            <b>• Nickcas-seq-F:</b><br />
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            TCTTTTGCGCTTCTTGATTAA<br />
 +
            <b>• Nickcas-seq-R:</b><br />
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            AAGTACTAAAACGGTAAC<br />
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            <b>• 2094-seq-F: </b><br />
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            GCTCTATAGCCTTTGATGC<br />
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            <b>• 2094-seq-R:</b><br />
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            CGCTAGTTATTTGTTCCCATCTG
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Latest revision as of 18:11, 19 October 2021

Shanghai_HS_ID

Safety
General Lab Safety
Our lab at ATLATL has the physical protection level of BSL-1, which means we only deal with microorganisms with no pathogenicity to healthy adults.
Fire Safety
There are fire hydrants, fire extinguishers, automatic spray system, manual fire alarm, fireproof shutter doors, and smoke alarms in the laboratory area. And the escaping route is well-managed. So our lab meets the national fire safety regulations.
Individual Protection
Eating or drinking is not allowed in the lab area. Besides, white coats and PVC gloves are required and only in the lab. Goggles are needed for hazardous experiments and an eye washer is provided for unexpected accidents.
Emergency Handling
Once the laboratory encounters a dangerous or unmanageable situation (such as experimental accidents, fires, leakage of dangerous goods, etc.), the person in charge of the laboratory will arrange for the relevant team to deal with it immediately. This laboratory is equipped with trained personnel to ensure a rapid response for any emergency.
Project Safety
Microorganism
We only used E. coli DH5α and Lactobacillus casei which are harmless to human beings and all related experiments were conducted under the supervision of our instructor, Ms. Song.
The plasmids we used in the project are listed below:
pLCNICK(Addgene, #84653)
pIB165(Addgene, #90188)
The primers we used in the project are listed below:
• Apal-334-2094-down-F：
CTAAGGATCACATCTTTTTCTAAACTAGGGCCCAACCAATCGTATCTTTGACAA
• BSAI-334 2094-down-R：
GATTAAACCTCCTTAAATTGCCCAGA GTACTCATTCC
• BSAI-334 2094-up-F：
TTTGGAGGAATTTAACGGGTCTCAGAGTAAGGATGCTAAAAAC
• BSAI-334 2094-up-R：
TTTTTTTGAGGAGACCCATGTTGTCTAGCTCAAGCA
• BSAI-334 2094-sgRNA-F：
TTGAGCTAGACAACATGGGTCTCCTCAAAAAAAGCACCGACT
• BSAI-334 2094-sgRNA-R1：
ACGATAAAGATCGAGATTTTGGCAATACAATACAACTATGCTGTC
• BSAI-334 2094-sgRNA-R2：
ATGCTGTCTAGATCTCCACTATAGTAGGAAAGAATACACG
• Nickcas-seq-F:
TCTTTTGCGCTTCTTGATTAA
• Nickcas-seq-R:
AAGTACTAAAACGGTAAC
• 2094-seq-F:
GCTCTATAGCCTTTGATGC
• 2094-seq-R:
CGCTAGTTATTTGTTCCCATCTG
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