(Prototype team page) |
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− | + | <html lang="en"> | |
− | + | ||
− | <html> | + | |
− | < | + | <head> |
+ | <meta charset="UTF-8"> | ||
+ | <meta http-equiv="X-UA-Compatible" content="IE=edge"> | ||
+ | <meta name="viewport" content="width=device-width, initial-scale=1.0"> | ||
+ | <title>Shanghai_HS_ID</title> | ||
+ | <link rel="stylesheet" | ||
+ | href="https://2021.igem.org/wiki/index.php?title=Template:Shanghai_HS_ID/Main_CSS&action=raw&ctype=text/css" /> | ||
− | + | </head> | |
− | + | ||
− | </div> | + | <body> |
− | <div class=" | + | <nav class="head-nav clearfix"> |
+ | <div class="top-block"></div> | ||
+ | <div class="top-nav-bar"> | ||
+ | <ul class="clearfix"> | ||
+ | <span class="small-logo"></span> | ||
+ | <li> | ||
+ | <a href="https://2021.igem.org/Team:Shanghai_HS_ID">Home</a> | ||
+ | </li> | ||
+ | <li class="active"> | ||
+ | <a href="">Project</a> | ||
+ | <div class="sub-nav"> | ||
+ | <ul> | ||
+ | <li><a href="https://2021.igem.org/Team:Shanghai_HS_ID/Description" | ||
+ | class="sub-nav-74">Description</a></li> | ||
+ | <li><a href="https://2021.igem.org/Team:Shanghai_HS_ID/Experiments" | ||
+ | class="sub-nav-74">Experiments</a></li> | ||
+ | <li><a href="https://2021.igem.org/Team:Shanghai_HS_ID/Results" | ||
+ | class="sub-nav-74">Results</a></li> | ||
+ | <li><a href="https://2021.igem.org/Team:Shanghai_HS_ID/Proof_Of_Concept" | ||
+ | class="sub-nav-52">Proof Of Concept</a></li> | ||
+ | <li class="current-sub-nav"><a href="#" class="sub-nav-52">Notebook</a></li> | ||
+ | <li><a href="https://2021.igem.org/Team:Shanghai_HS_ID/Safety">Safety</a></li> | ||
+ | </ul> | ||
+ | </div> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="">Parts</a> | ||
+ | <div class="sub-nav"> | ||
+ | <ul> | ||
+ | <li><a href="https://2021.igem.org/Team:Shanghai_HS_ID/Parts" class="sub-nav-74">Parts | ||
+ | Collection</a></li> | ||
+ | <li><a href="https://2021.igem.org/Team:Shanghai_HS_ID/Engineering" | ||
+ | class="sub-nav-74">Engineering</a></li> | ||
+ | <li><a href="https://2021.igem.org/Team:Shanghai_HS_ID/Contribution" | ||
+ | class="sub-nav-74">Contribution</a></li> | ||
+ | </ul> | ||
+ | </div> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="">Human Practices</a> | ||
+ | <div class="sub-nav"> | ||
+ | <ul> | ||
+ | <li><a href="https://2021.igem.org/Team:Shanghai_HS_ID/Human_Practices" | ||
+ | class="sub-nav-74">Integrated Human Practice</a></li> | ||
+ | <li><a href="https://2021.igem.org/Team:Shanghai_HS_ID/Communication" | ||
+ | class="sub-nav-74">Communication</a></li> | ||
+ | <li><a href="https://2021.igem.org/Team:Shanghai_HS_ID/Fundraising" | ||
+ | class="sub-nav-74">Fundraising</a></li> | ||
+ | </ul> | ||
+ | </div> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://2021.igem.org/Team:Shanghai_HS_ID/Implementation">Implementation</a> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://2021.igem.org/Team:Shanghai_HS_ID/Entrepreneurship">Entrepreneurship</a> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://2021.igem.org/Team:Shanghai_HS_ID/Model">Model</a> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="">Team</a> | ||
+ | <div class="sub-nav"> | ||
+ | <ul> | ||
+ | <li><a href="https://2021.igem.org/Team:Shanghai_HS_ID/Members" class="sub-nav-74">Team | ||
+ | Members</a></li> | ||
+ | <li><a href="https://2021.igem.org/Team:Shanghai_HS_ID/Attributions" | ||
+ | class="sub-nav-74">Attributions</a></li> | ||
+ | <li><a href="https://2021.igem.org/Team:Shanghai_HS_ID/Collaborations" | ||
+ | class="sub-nav-74">Collaborations</a></li> | ||
+ | <li><a href="https://2021.igem.org/Team:Shanghai_HS_ID/Partnership" | ||
+ | class="sub-nav-74">Partnership</a></li> | ||
+ | </ul> | ||
+ | </div> | ||
+ | </li> | ||
+ | </ul> | ||
+ | </div> | ||
+ | </nav> | ||
+ | <div class="sub-banner"> | ||
+ | <img src="https://static.igem.org/mediawiki/2021/c/c3/T--Shanghai_HS_ID--banner.jpg" alt="" /> | ||
+ | </div> | ||
+ | <div class="sub-content"> | ||
+ | <div class="sub-title">NOTEBOOK</div> | ||
+ | <div class="article-content">27<sup>th</sup> June: We had our first team meeting and discussed our topic</div> | ||
+ | <div class="article-content">28<sup>th</sup> June: We made the work distribution and finalized the designs of | ||
+ | the team name, logo, and merch. Besides, we conducted the interview with an expert.</div> | ||
+ | <div class="img-wrap no-margin"> | ||
+ | <img src="https://static.igem.org/mediawiki/2021/5/5f/T--Shanghai_HS_ID--Notebook01.jpg" alt="" /> | ||
+ | </div> | ||
+ | <div class="article-content"><b>Wet team agenda</b></div> | ||
+ | <div class="article-content">28<sup>th</sup> June:</div> | ||
+ | <div class="article-content">• Lab safety training</div> | ||
+ | <div class="img-wrap no-margin"> | ||
+ | <img src="https://static.igem.org/mediawiki/2021/2/2a/T--Shanghai_HS_ID--Notebook03.jpg" alt="" /> | ||
+ | </div> | ||
+ | <div class="article-content">30<sup>th</sup> June- 2<sup>nd</sup> July:</div> | ||
+ | <div class="article-content"> | ||
+ | • Construction of sgRNA, upstream and downstream homologous arms <br /> | ||
+ | • Overlap PCR and electrophoresis<br /> | ||
+ | • Preparation of mediums<br /> | ||
+ | • Bacteria culture | ||
+ | </div> | ||
+ | <div class="img-wrap no-margin"> | ||
+ | <img src="https://static.igem.org/mediawiki/2021/a/a9/T--Shanghai_HS_ID--Notebook04.jpg" alt="" /> | ||
+ | </div> | ||
+ | <div class="article-content">3<sup>rd</sup> July:</div> | ||
+ | <div class="article-content"> | ||
+ | • pLCNICK enzyme digestion <br /> | ||
+ | • Connection of sgRNA and pLCNICK<br /> | ||
+ | • Transformation of the recombinant plasmid into E.coli | ||
+ | </div> | ||
+ | <div class="img-wrap no-margin"> | ||
+ | <img src="https://static.igem.org/mediawiki/2021/6/66/T--Shanghai_HS_ID--Notebook05.jpg" alt="" /> | ||
+ | </div> | ||
+ | <div class="article-content">4<sup>th</sup> July:</div> | ||
+ | <div class="article-content"> | ||
+ | • Culture L. casei cells<br /> | ||
+ | • Preparation of competent L. casei cells | ||
+ | </div> | ||
+ | <div class="img-wrap no-margin"> | ||
+ | <img src="https://static.igem.org/mediawiki/2021/2/2a/T--Shanghai_HS_ID--Notebook06.jpg" alt="" /> | ||
+ | </div> | ||
+ | <div class="article-content">5<sup>th</sup> July:</div> | ||
+ | <div class="article-content"> | ||
+ | • Picked and shakes L. casei<br /> | ||
+ | • L. Casei PCR and electrophoresis | ||
+ | </div> | ||
+ | <div class="img-wrap no-margin"> | ||
+ | <img src="https://static.igem.org/mediawiki/2021/8/8d/T--Shanghai_HS_ID--Notebook08.jpg" alt="" /> | ||
+ | </div> | ||
+ | <div class="article-content">6<sup>th</sup>-7<sup>th</sup> July : </div> | ||
+ | <div class="article-content"> | ||
+ | • Verification of the plasmid<br /> | ||
+ | • Electrotransformation of the plasmid into L. casei ATCC 533 | ||
+ | August an September<br /> | ||
+ | • Repeat the plamid construction and electrotransformation<br /> | ||
+ | • L. casei cultivation | ||
− | + | </div> | |
− | + | <div class="img-wrap no-margin"> | |
− | <div class=" | + | <img src="https://static.igem.org/mediawiki/2021/7/74/T--Shanghai_HS_ID--Notebook09.jpg" alt="" /> |
− | < | + | </div> |
− | < | + | <div class="article-title">Dry team agenda </div> |
− | < | + | <div class="article-content">28<sup>th</sup> June: </div> |
− | < | + | <div class="article-content">• Published the first article of our Wechat official account: introducing us and |
− | < | + | our topic.</div> |
− | < | + | <div class="img-wrap no-margin"> |
− | </ | + | <img src="https://static.igem.org/mediawiki/2021/d/d9/T--Shanghai_HS_ID--Notebook10.jpg" alt="" /> |
− | + | </div> | |
− | </div> | + | <div class="article-content">30<sup>th</sup> June:</div> |
− | + | <div class="article-content"> | |
− | <div class=" | + | • Discussed the script of our promotion video <br /> |
− | <div class=" | + | • Listed survey questions and interview questions |
− | < | + | </div> |
− | < | + | <div class="img-wrap no-margin"> |
− | + | <img src="https://static.igem.org/mediawiki/2021/f/f4/T--Shanghai_HS_ID--Notebook11.jpg" alt="" /> | |
− | < | + | </div> |
− | < | + | <div class="article-content">1<sup>st</sup> July : </div> |
− | + | <div class="article-content"> | |
− | < | + | • Organized and analyzed the records of the 1st Expert Interview<br /> |
− | < | + | • Gathered information for our topic background<br /> |
− | + | • Prepared a question list for the site visit to Bright Dairy & Food company in the next day<br /> | |
− | + | • Assigned tasks for the next day's site visit | |
− | < | + | </div> |
− | < | + | <div class="article-content">2<sup>nd</sup> July : </div> |
− | < | + | <div class="article-content"> |
− | < | + | • Data analysis on survey results<br /> |
− | </ | + | • Integrated all interview records of the past few days<br /> |
− | </ | + | • Prepared questions for the 2nd Expert Interview<br /> |
− | </ | + | • Drafted a new article of our public Wechat account for the site visit<br /> |
+ | • Ordered merch: notebooks<br /> | ||
+ | • Designed post-it notes<br /> | ||
+ | • organize stuff for the article for the merch sale | ||
+ | </div> | ||
+ | <div class="img-wrap no-margin"> | ||
+ | <img src="https://static.igem.org/mediawiki/2021/f/f4/T--Shanghai_HS_ID--Notebook12.jpg" alt="" /> | ||
+ | </div> | ||
+ | <div class="article-content">3<sup>rd</sup> July : </div> | ||
+ | <div class="article-content"> | ||
+ | • Drafted self-introductions<br /> | ||
+ | • Drafted integrated human practices (transcription and translation of interview records, data of our Wechat | ||
+ | official account and survey results)<br /> | ||
+ | • Booked another interview<br /> | ||
+ | • Listed interview questions<br /> | ||
+ | • Publish the second article of our Wechat official account, which was focused on our site visit to Bright | ||
+ | Dairy & Food company. | ||
+ | </div> | ||
+ | <div class="article-content">4<sup>th</sup> July : </div> | ||
+ | <div class="article-content"> | ||
+ | • Drafted inspiration<br /> | ||
+ | • Discussed to determine the lecture location and target audience<br /> | ||
+ | • Promotion video filming<br /> | ||
+ | • Roll Screen design<br /> | ||
+ | • Visit BFC for merch-sale site pre-check | ||
+ | </div> | ||
+ | <div class="img-wrap no-margin"> | ||
+ | <img src="https://static.igem.org/mediawiki/2021/4/4a/T--Shanghai_HS_ID--Notebook13.jpg" alt="" /> | ||
+ | </div> | ||
+ | <div class="article-content">5<sup>th</sup> July : </div> | ||
+ | <div class="article-content"> | ||
+ | • Finalized IHP and inspiration<br /> | ||
+ | • Finalized details and publicize charity sale<br /> | ||
+ | • Started writing implementation and business plan<br /> | ||
+ | • Promotion video filming | ||
+ | </div> | ||
+ | <div class="img-wrap no-margin"> | ||
+ | <img src="https://static.igem.org/mediawiki/2021/f/f0/T--Shanghai_HS_ID--Notebook14.jpg" alt="" /> | ||
+ | </div> | ||
+ | <div class="article-content">6<sup>th</sup> July : </div> | ||
+ | <div class="article-content"> | ||
+ | • Drafted attributions, business plan<br /> | ||
+ | • Initially completed the script of our promotion video | ||
+ | </div> | ||
+ | <div class="img-wrap no-margin"> | ||
+ | <img src="https://static.igem.org/mediawiki/2021/5/5b/T--Shanghai_HS_ID--Notebook15.jpg" alt="" /> | ||
+ | </div> | ||
+ | <div class="article-content">7<sup>th</sup> July : </div> | ||
+ | <div class="article-content"> | ||
+ | • Planed for another charity sale<br /> | ||
+ | • Started writing a new article for merch sale to be published in our Wechat official account<br /> | ||
+ | • Collaboration activity<br /> | ||
+ | • Built our cost structure<br /> | ||
+ | • Finalized the script for promotion video<br /> | ||
+ | • First merch sale: yielded a profit of 50 RMB around and got 13 more followers | ||
+ | </div> | ||
+ | <div class="img-wrap no-margin"> | ||
+ | <img src="https://static.igem.org/mediawiki/2021/c/cd/T--Shanghai_HS_ID--Notebook16.jpg" alt="" /> | ||
+ | </div> | ||
+ | <div class="article-content">8<sup>th</sup> July : </div> | ||
+ | <div class="article-content"> | ||
+ | • Drafted future plan, safety, public engagement, and education<br /> | ||
+ | • Organized what we have done and drafted the first version of the presentation PowerPoint<br /> | ||
+ | • Fundraising | ||
+ | </div> | ||
+ | <div class="img-wrap no-margin"> | ||
+ | <img src="https://static.igem.org/mediawiki/2021/c/c9/T--Shanghai_HS_ID--Notebook17.jpg" alt="" /> | ||
+ | </div> | ||
+ | <div class="article-content">11<sup>th</sup> July : </div> | ||
+ | <div class="article-content"> | ||
+ | • Published the article about our merch sale in Wechat official account<br /> | ||
+ | • Determine the sale day online for our merch | ||
+ | </div> | ||
+ | </div> | ||
+ | <footer class="footer"> | ||
+ | <section class="footer-wrap"> | ||
+ | <div class="footer-contact">Follow us on WeChat!</div> | ||
+ | <img class="footer-qrcode" src="https://static.igem.org/mediawiki/2021/c/c3/T--Shanghai_HS_ID--qrcode.png" /> | ||
+ | <p class="margin-bottom-10"><i>WeChat Official Account: 乳酸LAB</i></p> | ||
+ | <p>Contact Email:<i>jhxchen@gmail.com</i></p> | ||
+ | </section> | ||
+ | </footer> | ||
+ | </body> | ||
+ | <script> | ||
+ | let liTags = document.querySelectorAll(".top-nav-bar > ul > li"); | ||
+ | let len = liTags.length; | ||
+ | for (let i = 0; i < len; i++) { | ||
+ | liTags[i].onclick = function (e) { | ||
+ | //先移除所有的点击样式 | ||
+ | for (let j = 0; j < len; j++) { | ||
+ | liTags[j].classList.remove("active"); | ||
+ | } | ||
+ | //再添加点击样式 | ||
+ | let li = e.currentTarget; | ||
+ | li.classList.add("active"); | ||
+ | } | ||
+ | } | ||
+ | </script> | ||
</html> | </html> |
Latest revision as of 18:09, 19 October 2021
NOTEBOOK
27th June: We had our first team meeting and discussed our topic
28th June: We made the work distribution and finalized the designs of
the team name, logo, and merch. Besides, we conducted the interview with an expert.
![](https://static.igem.org/mediawiki/2021/5/5f/T--Shanghai_HS_ID--Notebook01.jpg)
Wet team agenda
28th June:
• Lab safety training
![](https://static.igem.org/mediawiki/2021/2/2a/T--Shanghai_HS_ID--Notebook03.jpg)
30th June- 2nd July:
• Construction of sgRNA, upstream and downstream homologous arms
• Overlap PCR and electrophoresis
• Preparation of mediums
• Bacteria culture
• Overlap PCR and electrophoresis
• Preparation of mediums
• Bacteria culture
![](https://static.igem.org/mediawiki/2021/a/a9/T--Shanghai_HS_ID--Notebook04.jpg)
3rd July:
• pLCNICK enzyme digestion
• Connection of sgRNA and pLCNICK
• Transformation of the recombinant plasmid into E.coli
• Connection of sgRNA and pLCNICK
• Transformation of the recombinant plasmid into E.coli
![](https://static.igem.org/mediawiki/2021/6/66/T--Shanghai_HS_ID--Notebook05.jpg)
4th July:
• Culture L. casei cells
• Preparation of competent L. casei cells
• Preparation of competent L. casei cells
![](https://static.igem.org/mediawiki/2021/2/2a/T--Shanghai_HS_ID--Notebook06.jpg)
5th July:
• Picked and shakes L. casei
• L. Casei PCR and electrophoresis
• L. Casei PCR and electrophoresis
![](https://static.igem.org/mediawiki/2021/8/8d/T--Shanghai_HS_ID--Notebook08.jpg)
6th-7th July :
• Verification of the plasmid
• Electrotransformation of the plasmid into L. casei ATCC 533 August an September
• Repeat the plamid construction and electrotransformation
• L. casei cultivation
• Electrotransformation of the plasmid into L. casei ATCC 533 August an September
• Repeat the plamid construction and electrotransformation
• L. casei cultivation
![](https://static.igem.org/mediawiki/2021/7/74/T--Shanghai_HS_ID--Notebook09.jpg)
Dry team agenda
28th June:
• Published the first article of our Wechat official account: introducing us and
our topic.
![](https://static.igem.org/mediawiki/2021/d/d9/T--Shanghai_HS_ID--Notebook10.jpg)
30th June:
• Discussed the script of our promotion video
• Listed survey questions and interview questions
• Listed survey questions and interview questions
![](https://static.igem.org/mediawiki/2021/f/f4/T--Shanghai_HS_ID--Notebook11.jpg)
1st July :
• Organized and analyzed the records of the 1st Expert Interview
• Gathered information for our topic background
• Prepared a question list for the site visit to Bright Dairy & Food company in the next day
• Assigned tasks for the next day's site visit
• Gathered information for our topic background
• Prepared a question list for the site visit to Bright Dairy & Food company in the next day
• Assigned tasks for the next day's site visit
2nd July :
• Data analysis on survey results
• Integrated all interview records of the past few days
• Prepared questions for the 2nd Expert Interview
• Drafted a new article of our public Wechat account for the site visit
• Ordered merch: notebooks
• Designed post-it notes
• organize stuff for the article for the merch sale
• Integrated all interview records of the past few days
• Prepared questions for the 2nd Expert Interview
• Drafted a new article of our public Wechat account for the site visit
• Ordered merch: notebooks
• Designed post-it notes
• organize stuff for the article for the merch sale
![](https://static.igem.org/mediawiki/2021/f/f4/T--Shanghai_HS_ID--Notebook12.jpg)
3rd July :
• Drafted self-introductions
• Drafted integrated human practices (transcription and translation of interview records, data of our Wechat official account and survey results)
• Booked another interview
• Listed interview questions
• Publish the second article of our Wechat official account, which was focused on our site visit to Bright Dairy & Food company.
• Drafted integrated human practices (transcription and translation of interview records, data of our Wechat official account and survey results)
• Booked another interview
• Listed interview questions
• Publish the second article of our Wechat official account, which was focused on our site visit to Bright Dairy & Food company.
4th July :
• Drafted inspiration
• Discussed to determine the lecture location and target audience
• Promotion video filming
• Roll Screen design
• Visit BFC for merch-sale site pre-check
• Discussed to determine the lecture location and target audience
• Promotion video filming
• Roll Screen design
• Visit BFC for merch-sale site pre-check
![](https://static.igem.org/mediawiki/2021/4/4a/T--Shanghai_HS_ID--Notebook13.jpg)
5th July :
• Finalized IHP and inspiration
• Finalized details and publicize charity sale
• Started writing implementation and business plan
• Promotion video filming
• Finalized details and publicize charity sale
• Started writing implementation and business plan
• Promotion video filming
![](https://static.igem.org/mediawiki/2021/f/f0/T--Shanghai_HS_ID--Notebook14.jpg)
6th July :
• Drafted attributions, business plan
• Initially completed the script of our promotion video
• Initially completed the script of our promotion video
![](https://static.igem.org/mediawiki/2021/5/5b/T--Shanghai_HS_ID--Notebook15.jpg)
7th July :
• Planed for another charity sale
• Started writing a new article for merch sale to be published in our Wechat official account
• Collaboration activity
• Built our cost structure
• Finalized the script for promotion video
• First merch sale: yielded a profit of 50 RMB around and got 13 more followers
• Started writing a new article for merch sale to be published in our Wechat official account
• Collaboration activity
• Built our cost structure
• Finalized the script for promotion video
• First merch sale: yielded a profit of 50 RMB around and got 13 more followers
![](https://static.igem.org/mediawiki/2021/c/cd/T--Shanghai_HS_ID--Notebook16.jpg)
8th July :
• Drafted future plan, safety, public engagement, and education
• Organized what we have done and drafted the first version of the presentation PowerPoint
• Fundraising
• Organized what we have done and drafted the first version of the presentation PowerPoint
• Fundraising
![](https://static.igem.org/mediawiki/2021/c/c9/T--Shanghai_HS_ID--Notebook17.jpg)
11th July :
• Published the article about our merch sale in Wechat official account
• Determine the sale day online for our merch
• Determine the sale day online for our merch