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+ | <head> | ||
+ | <meta charset="UTF-8"> | ||
+ | <meta http-equiv="X-UA-Compatible" content="IE=edge"> | ||
+ | <meta name="viewport" content="width=device-width, initial-scale=1.0"> | ||
+ | <title>SHSID</title> | ||
+ | <link rel="stylesheet" | ||
+ | href="https://2021.igem.org/wiki/index.php?title=Template:SHSID/Main_CSS&action=raw&ctype=text/css" /> | ||
+ | <link rel="stylesheet" | ||
+ | href="https://2021.igem.org/wiki/index.php?title=Template:Shanghai_United_HS/Swiper_CSS&action=raw&ctype=text/css"> | ||
+ | </head> | ||
+ | <body> | ||
+ | <nav class="head-nav clearfix"> | ||
+ | <div class="top-block"></div> | ||
+ | <div class="top-nav-bar"> | ||
+ | <ul class="clearfix"> | ||
+ | <span class="small-logo"></span> | ||
+ | <li class="active"> | ||
+ | <a href="#">Home</a> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="">Project</a> | ||
+ | <div class="sub-nav"> | ||
+ | <ul> | ||
+ | <li><a href="https://2021.igem.org/Team:SHSID/Description" | ||
+ | class="sub-nav-74">Description</a></li> | ||
+ | <li><a href="https://2021.igem.org/Team:SHSID/Experiments" | ||
+ | class="sub-nav-74">Experiments</a></li> | ||
+ | <li><a href="https://2021.igem.org/Team:SHSID/Results" class="sub-nav-74">Results</a></li> | ||
+ | <li><a href="https://2021.igem.org/Team:SHSID/Proof_Of_Concept" class="sub-nav-52">Proof Of | ||
+ | Concept</a></li> | ||
+ | <li><a href="https://2021.igem.org/Team:SHSID/Notebook" class="sub-nav-52">Notebook</a></li> | ||
+ | <li><a href="https://2021.igem.org/Team:SHSID/Safety">Safety</a></li> | ||
+ | </ul> | ||
+ | </div> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="">Parts</a> | ||
+ | <div class="sub-nav"> | ||
+ | <ul> | ||
+ | <li><a href="https://2021.igem.org/Team:SHSID/Parts" class="sub-nav-74">Parts | ||
+ | Collection</a></li> | ||
+ | <li><a href="https://2021.igem.org/Team:SHSID/Engineering" | ||
+ | class="sub-nav-74">Engineering</a></li> | ||
+ | <li><a href="https://2021.igem.org/Team:SHSID/Contribution" | ||
+ | class="sub-nav-74">Contribution</a></li> | ||
+ | </ul> | ||
+ | </div> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="">Human Practices</a> | ||
+ | <div class="sub-nav"> | ||
+ | <ul> | ||
+ | <li><a href="https://2021.igem.org/Team:SHSID/Human_Practices" class="sub-nav-74">Integrated | ||
+ | Human Practice</a></li> | ||
+ | <li><a href="https://2021.igem.org/Team:SHSID/Communication" | ||
+ | class="sub-nav-74">Communication</a></li> | ||
+ | <li><a href="https://2021.igem.org/Team:SHSID/Fundraising" | ||
+ | class="sub-nav-74">Fundraising</a></li> | ||
+ | </ul> | ||
+ | </div> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://2021.igem.org/Team:SHSID/Implementation">Implementation</a> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://2021.igem.org/Team:SHSID/Entrepreneurship">Entrepreneurship</a> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="https://2021.igem.org/Team:SHSID/Model">Model</a> | ||
+ | </li> | ||
+ | <li> | ||
+ | <a href="">Team</a> | ||
+ | <div class="sub-nav"> | ||
+ | <ul> | ||
+ | <li><a href="https://2021.igem.org/Team:SHSID/Members" class="sub-nav-74">Team | ||
+ | Members</a></li> | ||
+ | <li><a href="https://2021.igem.org/Team:SHSID/Attributions" | ||
+ | class="sub-nav-74">Attributions</a></li> | ||
+ | <li><a href="https://2021.igem.org/Team:SHSID/Collaborations" | ||
+ | class="sub-nav-74">Collaborations</a></li> | ||
+ | </ul> | ||
+ | </div> | ||
+ | </li> | ||
+ | </ul> | ||
+ | </div> | ||
+ | </nav> | ||
+ | <div class="home-content"> | ||
+ | <div class="top-banner"> | ||
+ | <img src="https://static.igem.org/mediawiki/2021/0/0f/T--SHSID--homepage01.gif" /> | ||
+ | </div> | ||
+ | <div class="swiper-container top-swiper-container group-photo"> | ||
+ | <div class="swiper-wrapper"> | ||
+ | <div class="swiper-slide"> | ||
+ | <img src="https://static.igem.org/mediawiki/2021/e/ee/T--SHSID--homepage02.png" /> | ||
+ | </div> | ||
+ | <div class="swiper-slide"> | ||
+ | <img src="https://static.igem.org/mediawiki/2021/5/5c/T--SHSID--homepage03.png" /> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="swiper-pagination top-swiper-pagination"></div> | ||
+ | </div> | ||
+ | <div class="team-tips"> | ||
+ | <p class="team-tips-title">ABSTRACT</p> | ||
+ | <div class="team-tips-wrap"> | ||
+ | <p class="team-tips-content"> | ||
+ | Tropane alkaloid-derived medicines are under supply shortage, but growing demand. The extraction of | ||
+ | putrescine from plants was time-consuming and low yields, which is seen as a vital limiting factor | ||
+ | of tropane alkaloids production. Our project aims at genetically modifying yeast to efficiently | ||
+ | produce putrescine in large quantities. On one hand, we designed to introduce the spe1gene to | ||
+ | over-express the enzyme that catalyzes the reaction producing putrescine from ornithine. On the | ||
+ | other hand, we also introduced two other genes, AsADC derived from oats and speB derived from E. | ||
+ | coli which could produce enzymes catalyzing the reaction producing putrescine from arginine. All | ||
+ | above will make the yeast a factory to continuously biosynthetic putrescine to support tropane | ||
+ | alkaloids production. | ||
+ | </p> | ||
+ | </div> | ||
+ | </div> | ||
+ | <video width="100%" height="769" controls class="home-video"> | ||
+ | <source src="https://static.igem.org/mediawiki/2021/a/a4/T--SHSID--homepage04.mp4" type="video/mp4"> | ||
+ | </video> | ||
+ | </div> | ||
+ | <footer class="footer"> | ||
+ | <section class="footer-wrap"> | ||
+ | <div class="contact-tips">Follow Us on WeChat and Instagram!</div> | ||
+ | <img src="https://static.igem.org/mediawiki/2021/5/53/T--SHSID--qrcode.jpg" /> | ||
+ | </section> | ||
+ | </footer> | ||
+ | </body> | ||
+ | <script | ||
+ | src="https://2021.igem.org/wiki/index.php?title=Template:Shanghai_United_HS/Swiper_JS&action=raw&ctype=text/javascript"> </script> | ||
+ | <script> | ||
+ | let liTags = document.querySelectorAll(".top-nav-bar > ul > li"); | ||
+ | let len = liTags.length; | ||
+ | for (let i = 0; i < len; i++) { | ||
+ | liTags[i].onclick = function (e) { | ||
+ | //先移除所有的点击样式 | ||
+ | for (let j = 0; j < len; j++) { | ||
+ | liTags[j].classList.remove("active"); | ||
+ | } | ||
+ | //再添加点击样式 | ||
+ | let li = e.currentTarget; | ||
+ | li.classList.add("active"); | ||
+ | } | ||
+ | } | ||
+ | var topSwiper = new Swiper('.top-swiper-container', { | ||
+ | loop: true, | ||
+ | autoplay: { | ||
+ | disableOnInteraction: false, | ||
+ | }, | ||
+ | speed: 2000, | ||
+ | pagination: { | ||
+ | el: ".top-swiper-pagination", | ||
+ | dynamicBullets: true, | ||
+ | } | ||
+ | }); | ||
+ | </script> | ||
</html> | </html> |
Latest revision as of 16:56, 19 October 2021
ABSTRACT
Tropane alkaloid-derived medicines are under supply shortage, but growing demand. The extraction of putrescine from plants was time-consuming and low yields, which is seen as a vital limiting factor of tropane alkaloids production. Our project aims at genetically modifying yeast to efficiently produce putrescine in large quantities. On one hand, we designed to introduce the spe1gene to over-express the enzyme that catalyzes the reaction producing putrescine from ornithine. On the other hand, we also introduced two other genes, AsADC derived from oats and speB derived from E. coli which could produce enzymes catalyzing the reaction producing putrescine from arginine. All above will make the yeast a factory to continuously biosynthetic putrescine to support tropane alkaloids production.