Team:SHSID/Contribution

SHSID

Contribution

BBa_K3993000

== Profile ==

Name: SPE1
Base Pairs: 1401bp
Origin: Saccharomyces cerevisiae, genome
Properties: Catalyzes the first and rate-limiting step of polyamine biosynthesis that converts ornithine into putrescine

==== Usage and Biology ====

his protein is involved in step 1 of the subpathway that synthesizes putrescine from L-ornithine. Catalyzes the first and rate-limiting step of polyamine biosynthesis that converts ornithine into putrescine, which is the precursor for the polyamines, spermidine and spermine. Polyamines are essential for cell proliferation and are implicated in cellular processes, ranging from DNA replication to apoptosis. Homodimer and only the dimer is catalytically active, as the active sites are constructed of residues from both monomers

Figure1. Principle diagram of TAs.

BBa_K3993001

== Profile ==

Name: SPEB
Base Pairs: 921bp
Origin: E. coli, genome
Properties: Catalyzes the formation of putrescine from agmatine.

BBa_K3993002

== Profile ==

Name: AsADC
Base Pairs: 1821bp
Origin: Saccharomyces cerevisiae, synthesis
Properties: codon optimized SPE1

BBa_K3993010

== Profile ==

Name: PTDH3-SPE1-TCYC1
Base Pairs: 2316bp
Origin: Saccharomyces cerevisiae, E. coli, synthesis
Properties: Arginine metabolism and polyamine biosynthesis chemical reactions

== Construct design ==

The Tropine part of Tropane alkaloids (TAs) is obtained from arginine to putrescine (1,4-butanediamine, putrescine), and then through a series of chemical reactions. In this project, natural genes involved in arginine metabolism and polyamine biosynthesis was designed to overexpress in yeast. The engineer strains that produced excess putrescine. (Figure 2).

Figure 2. DNA sequence map of plasmid PTDH3-SPE1-TCYC1.

BBa_K3993011

== Profile ==

Name: PTDH3-speB-TCYC1
Base Pairs: 1836bp
Origin: Saccharomyces cerevisiae, E. coli, synthesis
Properties: Arginine metabolism and polyamine biosynthesis chemical reactions

== Construct design ==

The Tropine part of Tropane alkaloids (TAs) is obtained from arginine to putrescine (1,4-butanediamine, putrescine), and then through a series of chemical reactions. In this project, natural genes involved in arginine metabolism and polyamine biosynthesis was designed to overexpress in yeast. The engineer strains that produced excess putrescine. (Figure 2).

Figure 2. DNA sequence map of plasmid PTDH3-SpeB-TCYC1.

BBa_K3993012

== Profile ==

Name: PTDH3-AsADC-TCYC1
Base Pairs: 2736bp
Origin: Saccharomyces cerevisiae, E. coli, synthesis
Properties: Arginine metabolism and polyamine biosynthesis chemical reactions

== Construct design ==

The Tropine part of Tropane alkaloids (TAs) is obtained from arginine to putrescine (1,4-butanediamine, putrescine), and then through a series of chemical reactions. In this project, natural genes involved in arginine metabolism and polyamine biosynthesis was designed to overexpress in yeast. The engineer strains that produced excess putrescine. (Figure 2).

Figure 2. DNA sequence map of plasmid PTDH3-AsADC-TCYC1.

BBa_K3993013

== Profile ==

Name: PPGK1-AsADC-TADH1 - PTEF1-SPE1-TTEF1 - PTDH3-speB-TCYC1
Base Pairs: 6866 bp
Origin: Saccharomyces cerevisiae, E. coli, synthesis, genome
Properties: Arginine metabolism and polyamine biosynthesis chemical reactions

== Construct design ==

The Tropine part of Tropane alkaloids (TAs) is obtained from arginine to putrescine (1,4-butanediamine, putrescine), and then through a series of chemical reactions. In this project, natural genes involved in arginine metabolism and polyamine biosynthesis was designed to overexpress in yeast. The engineer strains that produced excess putrescine. (Figure 2).

Figure 2. DNA sequence map of plasmid pYES2-AsADC-SPE1-SpeB.