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<body> | <body> | ||
− | + | <div style="height: 255px;width: 100%;display: flex;justify-content: space-around;"> | |
− | + | <img src="https://static.igem.org/mediawiki/2021/2/27/T--Shanghai_United--homepage01.png" | |
− | + | style="width: 173px; height: 89px;margin-right: 271px;margin-left: 120px;margin-top: 80px;display: block;" /> | |
− | + | <ul class="layui-nav navbar navbar-fixed-top text-center" lay-filter="" | |
− | + | style="background-color:white;height: 255px;line-height: 255px;"> | |
− | + | <li class="layui-nav-item" style="text-align: center;"> | |
− | + | <a href="https://2021.igem.org/Team:Shanghai_United" | |
− | + | style="text-align: center;">Home</a> | |
− | + | </li> | |
− | + | <li class="layui-nav-item" style="text-align: center;"> | |
− | + | <a href="javascript:;">Project</a> | |
− | + | <dl class="layui-nav-child"> | |
− | + | <!-- 二级菜单 --> | |
− | + | <dd> | |
− | + | <a | |
− | + | href="https://2021.igem.org/Team:Shanghai_United/Description">Description</a> | |
− | + | </dd> | |
− | + | <dd> | |
− | + | <a href="https://2021.igem.org/Team:Shanghai_United/Proof_Of_Concept">Proof | |
− | + | of Concept</a> | |
− | + | </dd> | |
− | + | <dd> | |
− | + | <a | |
− | + | href="https://2021.igem.org/Team:Shanghai_United/Experiments">Experiments</a> | |
− | + | </dd> | |
− | + | <dd> | |
− | + | <a | |
− | + | href="https://2021.igem.org/Team:Shanghai_United/Notebook">Notebook</a> | |
− | + | </dd> | |
− | + | <dd> | |
− | + | <a href="https://2021.igem.org/Team:Shanghai_United/Results">Results</a> | |
− | + | </dd> | |
− | + | <dd> | |
− | + | <a href="https://2021.igem.org/Team:Shanghai_United/Safety">Safety</a> | |
− | + | </dd> | |
− | + | ||
− | + | ||
− | + | ||
− | + | </dl> | |
− | + | </li> | |
− | + | <li class="layui-nav-item" style="text-align: center;"> | |
− | + | <a href="javascript:;">Parts</a> | |
− | + | <dl class="layui-nav-child"> | |
− | + | <!-- 二级菜单 --> | |
− | + | <dd> | |
− | + | <a | |
− | + | href="https://2021.igem.org/Team:Shanghai_United/Engineering">Engineering</a> | |
− | + | </dd> | |
− | + | <dd> | |
− | + | <a href="https://2021.igem.org/Team:Shanghai_United/Parts">Parts | |
− | + | Collection</a> | |
− | + | </dd> | |
− | + | </dl> | |
− | + | </li> | |
− | + | <li class="layui-nav-item"> | |
− | + | <a href="javascript:;">Human Practice</a> | |
− | + | <dl class="layui-nav-child"> | |
− | + | <!-- 二级菜单 --> | |
− | + | <dd> | |
− | + | <a | |
− | + | href="https://2021.igem.org/Team:Shanghai_United/Fundraising">Fundraising</a> | |
− | + | </dd> | |
− | + | <dd> | |
− | + | <a | |
− | + | href="https://2021.igem.org/Team:Shanghai_United/Communication">Communication</a> | |
− | + | </dd> | |
− | + | <dd> | |
− | + | <a | |
− | + | href="https://2021.igem.org/Team:Shanghai_United/Human_Practices">Integrated Human Practices</a> | |
− | + | </dd> | |
− | + | </dl> | |
− | + | </li> | |
− | + | <li class="layui-nav-item" style="text-align: center;"> | |
− | + | <a href="https://2021.igem.org/Team:Shanghai_United/Implementation">Implementation</a> | |
− | + | </li> | |
− | + | <li class="layui-nav-item" style="text-align: center;"> | |
− | + | <a | |
− | + | href="https://2021.igem.org/Team:Shanghai_United/Entrepreneurship">Entrepreneurship</a> | |
− | + | </li> | |
− | + | <li class="layui-nav-item" style="text-align: center;"> | |
− | + | <a href="https://2021.igem.org/Team:Shanghai_United/Model">Model</a> | |
− | + | </li> | |
− | + | <li class="layui-nav-item" style="text-align: center;"> | |
− | + | <a href="javascript:;">Team</a> | |
− | + | <dl class="layui-nav-child"> | |
− | + | <!-- 二级菜单 --> | |
− | + | <dd> | |
− | + | <a href="https://2021.igem.org/Team:Shanghai_United/Team">Team | |
− | + | Member</a> | |
− | + | </dd> | |
− | + | <dd> | |
− | + | <a | |
− | + | href="https://2021.igem.org/Team:Shanghai_United/Collaborations">Collaborations</a> | |
− | + | </dd> | |
− | + | <dd> | |
− | + | <a | |
− | + | href="https://2021.igem.org/Team:Shanghai_United/Attributions">Attributions</a> | |
− | + | </dd> | |
− | + | </dl> | |
− | + | </li> | |
− | + | </ul> | |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | </div> | |
<!--banner--> | <!--banner--> | ||
− | <div style="width:100%;"> | + | <div style="width:100%;position: relative;"> |
− | + | <img style="width:100%;" src="https://static.igem.org/mediawiki/2021/9/95/T--Shanghai_United--homepage08.jpg"></img> | |
− | + | <p style="color:white;font-size:48px;position: absolute;font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:48px; font-weight:bold;display: block;bottom: 100px;left: 100px;">Safety</p> | |
+ | </div> | ||
<!--text--> | <!--text--> | ||
− | <div style="width:100%;padding: | + | <div style="width:100%;padding:5% 30%;"> |
<div style="width:100%;"> | <div style="width:100%;"> | ||
− | <p style="font-size:36px; line-height: | + | <p style="font-size:36px; line-height:200%; margin:50px 0pt; orphans:0; widows:0"><span |
style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:36px; font-weight:bold; ">Basic | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:36px; font-weight:bold; ">Basic | ||
</span><span | </span><span | ||
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Requirements</span></p> | Requirements</span></p> | ||
<p | <p | ||
− | style="font-size:22px; line-height: | + | style="font-size:22px; line-height:200%; margin:3pt 0pt; orphans:0; text-align:justify; text-indent:40px; widows:0"> |
<span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">All personal items were stored in | <span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">All personal items were stored in | ||
a separate meeting room and were not allowed to be brought into the laboratory, with the only exceptions | a separate meeting room and were not allowed to be brought into the laboratory, with the only exceptions | ||
being a notebook and a writing utensil. </span></p> | being a notebook and a writing utensil. </span></p> | ||
<p | <p | ||
− | style="font-size:22px; line-height: | + | style="font-size:22px; line-height:200%; margin:3pt 0pt; orphans:0; text-align:justify; text-indent:40px; widows:0"> |
<span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Latex gloves, masks, and lab | <span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Latex gloves, masks, and lab | ||
coats were required before entering the laborat</span><span | coats were required before entering the laborat</span><span | ||
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stored inside the laboratory before leaving the lab. </span></p> | stored inside the laboratory before leaving the lab. </span></p> | ||
<p | <p | ||
− | style="font-size:22px; line-height: | + | style="font-size:22px; line-height:200%; margin:3pt 0pt; orphans:0; text-align:justify; text-indent:40px; widows:0"> |
<span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">All used materials must be | <span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">All used materials must be | ||
discarded in designated medical waste containers. Latex gloves and masks were ch</span><span | discarded in designated medical waste containers. Latex gloves and masks were ch</span><span | ||
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style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">. </span></p> | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">. </span></p> | ||
<p | <p | ||
− | style="font-size:22px; line-height: | + | style="font-size:22px; line-height:200%; margin:3pt 0pt; orphans:0; text-align:justify; text-indent:40px; widows:0"> |
<span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">All team members were expected to | <span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">All team members were expected to | ||
learn </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">the </span><span | learn </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">the </span><span | ||
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</span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">emergency, members must be | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">emergency, members must be | ||
evacuated through these routes. </span></p> | evacuated through these routes. </span></p> | ||
− | <p style="font-size:22px; line-height: | + | <p style="font-size:22px; line-height:200%; margin:3pt 0pt; orphans:0; text-indent:40px; widows:0"><span |
style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px"> </span></p> | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px"> </span></p> | ||
− | <p style="font-size:36px; line-height: | + | <p style="font-size:36px; line-height:200%; margin:50px 0pt; orphans:0; text-align:justify; widows:0"><span |
style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:36px; font-weight:bold; ">Equipment | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:36px; font-weight:bold; ">Equipment | ||
Precautions</span></p> | Precautions</span></p> | ||
− | <p style="font-size:22px; line-height: | + | <p style="font-size:22px; line-height:200%; margin:3pt 0pt; orphans:0; text-align:justify; widows:0"><span |
style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">Centrifuge: | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">Centrifuge: | ||
</span></p> | </span></p> | ||
<p | <p | ||
− | style="font-size:22px; line-height: | + | style="font-size:22px; line-height:200%; margin:3pt 0pt 3pt 21pt; orphans:0; text-align:justify; text-indent:40px; widows:0"> |
<span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">The centrifuge must be balanced | <span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">The centrifuge must be balanced | ||
when used: when a test tube is being centrifuged at a specific slot, another test tube with | when used: when a test tube is being centrifuged at a specific slot, another test tube with | ||
approximately the same mass must be placed at a complimentary slot. </span></p> | approximately the same mass must be placed at a complimentary slot. </span></p> | ||
<p | <p | ||
− | style="font-size:22px; line-height: | + | style="font-size:22px; line-height:200%; margin:3pt 0pt 3pt 21pt; orphans:0; text-align:justify; text-indent:40px; widows:0"> |
<span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">The metal lid of the centrifuge | <span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">The metal lid of the centrifuge | ||
must be locked onto the rotatio</span><span | must be locked onto the rotatio</span><span | ||
style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">n plate for protection in the event | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">n plate for protection in the event | ||
of a test tube being ejected at high velocity from the plate. </span></p> | of a test tube being ejected at high velocity from the plate. </span></p> | ||
− | <p style="font-size:22px; line-height: | + | <p style="font-size:22px; line-height:200%; margin-top: 30px; orphans:0; text-align:justify; widows:0"><span |
style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">Alcohol burner: | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">Alcohol burner: | ||
</span></p> | </span></p> | ||
<p | <p | ||
− | style="font-size:22px; line-height: | + | style="font-size:22px; line-height:200%; margin:3pt 0pt 3pt 21pt; orphans:0; text-align:justify; text-indent:40px; widows:0"> |
<span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">A burner must be lit by matches | <span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">A burner must be lit by matches | ||
or fire starters. A lit burner must not be used to light other burners. </span></p> | or fire starters. A lit burner must not be used to light other burners. </span></p> | ||
<p | <p | ||
− | style="font-size:22px; line-height: | + | style="font-size:22px; line-height:200%; margin:3pt 0pt 3pt 21pt; orphans:0; text-align:justify; text-indent:40px; widows:0"> |
<span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">A burner must be put out by its | <span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">A burner must be put out by its | ||
lid</span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">, and must not be | lid</span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">, and must not be | ||
put out through blowing or other means. </span></p> | put out through blowing or other means. </span></p> | ||
<p | <p | ||
− | style="font-size:22px; line-height: | + | style="font-size:22px; line-height:200%; margin:3pt 0pt 3pt 21pt; orphans:0; text-align:justify; text-indent:40px; widows:0"> |
<span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">A burner is considered a fire and | <span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">A burner is considered a fire and | ||
therefore must be handled with care. </span></p> | therefore must be handled with care. </span></p> | ||
<p | <p | ||
− | style="font-size:22px; line-height: | + | style="font-size:22px; line-height:200%; margin:3pt 0pt 3pt 21pt; orphans:0; text-align:justify; text-indent:40px; widows:0"> |
<span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">An alcohol burner should not | <span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">An alcohol burner should not | ||
contain more than one-third of alcohol to prevent </span><span | contain more than one-third of alcohol to prevent </span><span | ||
style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">any fire risk</span><span | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">any fire risk</span><span | ||
style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">.</span></p> | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">.</span></p> | ||
− | <p style="font-size:22px; line-height: | + | <p style="font-size:22px; line-height:200%; margin-top: 30px; orphans:0; text-align:justify; widows:0"><span |
style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">Micropipettes: | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">Micropipettes: | ||
</span></p> | </span></p> | ||
<p | <p | ||
− | style="font-size:22px; line-height: | + | style="font-size:22px; line-height:200%; margin:3pt 0pt 3pt 21pt; orphans:0; text-align:justify; text-indent:40px; widows:0"> |
<span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Micropipettes t</span><span | <span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Micropipettes t</span><span | ||
style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">hat are purposed for RNA and DNA | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">hat are purposed for RNA and DNA | ||
Line 310: | Line 304: | ||
style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">d contamination. </span></p> | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">d contamination. </span></p> | ||
<p | <p | ||
− | style="font-size:22px; line-height: | + | style="font-size:22px; line-height:200%; margin:3pt 0pt 3pt 21pt; orphans:0; text-align:justify; text-indent:40px; widows:0"> |
<span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Micropipettes must not be held | <span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Micropipettes must not be held | ||
upside-down; failure to do so may result in contamination and damage to the equipment. </span></p> | upside-down; failure to do so may result in contamination and damage to the equipment. </span></p> | ||
<p | <p | ||
− | style="font-size:22px; line-height: | + | style="font-size:22px; line-height:200%; margin:3pt 0pt 3pt 21pt; orphans:0; text-align:justify; text-indent:40px; widows:0"> |
<span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Micropipette tips are single use | <span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Micropipette tips are single use | ||
only, and must not touch</span><span | only, and must not touch</span><span | ||
Line 320: | Line 314: | ||
surfaces. </span></p> | surfaces. </span></p> | ||
<p | <p | ||
− | style="font-size:22px; line-height: | + | style="font-size:22px; line-height:200%; margin:3pt 0pt 3pt 21pt; orphans:0; text-align:justify; text-indent:40px; widows:0"> |
<span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">After using a micropipette, the | <span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">After using a micropipette, the | ||
equipment must be adjusted to its maximum volume to preserve the equipment. </span></p> | equipment must be adjusted to its maximum volume to preserve the equipment. </span></p> | ||
− | <p style="font-size:22px; line-height: | + | <p style="font-size:22px; line-height:200%; margin-top: 30px; orphans:0; text-align:justify; widows:0"><span |
style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">Water bath: | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">Water bath: | ||
</span></p> | </span></p> | ||
<p | <p | ||
− | style="font-size:22px; line-height: | + | style="font-size:22px; line-height:200%; orphans:0; text-align:justify; text-indent:40px; widows:0"> |
<span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Water baths must be used when | <span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Water baths must be used when | ||
filled with water. Failure to do so may result in fire hazards. </span></p> | filled with water. Failure to do so may result in fire hazards. </span></p> | ||
− | <p style="font-size:22px; line-height: | + | <p style="font-size:22px; line-height:200%; margin:3pt 0pt; orphans:0; text-align:justify; widows:0"><span |
style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px"> </span></p> | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px"> </span></p> | ||
− | <p style="font-size:36px; line-height: | + | <p style="font-size:36px; line-height:200%; margin:50px 0pt; orphans:0; text-align:justify; widows:0"><span |
style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:36px; font-weight:bold; ">Other | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:36px; font-weight:bold; ">Other | ||
precautions</span></p> | precautions</span></p> | ||
<p | <p | ||
− | style="font-size:22px; line-height: | + | style="font-size:22px; line-height:200%; margin:3pt 0pt; orphans:0; text-align:justify; text-indent:40px; widows:0"> |
<span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Due to the Covid-19 pandemic, all | <span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Due to the Covid-19 pandemic, all | ||
members were tested prior to entering the laboratory; only members with negative tests were allowed | members were tested prior to entering the laboratory; only members with negative tests were allowed | ||
in.</span></p> | in.</span></p> | ||
− | <p style="font-size:22px; line-height: | + | <p style="font-size:22px; line-height:200%; margin:3pt 0pt; orphans:0; text-align:justify; widows:0"><span |
style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px"> </span></p> | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px"> </span></p> | ||
− | <p style="font-size:36px; line-height: | + | <p style="font-size:36px; line-height:200%; margin:50px 0pt; orphans:0; text-align:justify; widows:0"><span |
style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:36px; font-weight:bold; ">M</span><span | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:36px; font-weight:bold; ">M</span><span | ||
style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:36px; font-weight:bold; ">icrobes</span><span | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:36px; font-weight:bold; ">icrobes</span><span | ||
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Safety</span></p> | Safety</span></p> | ||
<p | <p | ||
− | style="font-size:22px; line-height: | + | style="font-size:22px; line-height:200%; margin:3pt 0pt; orphans:0; text-align:justify; text-indent:40px; widows:0"> |
<span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">In this project, we only use a | <span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">In this project, we only use a | ||
safe and common lab strain, Escherichia coli DH5-α</span><span | safe and common lab strain, Escherichia coli DH5-α</span><span | ||
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Weibo、Bilibili、Wechat ! | Weibo、Bilibili、Wechat ! | ||
</span> | </span> | ||
− | <img style="width:810px;height: 345px;margin: 100px auto;display: block;" src="https://static.igem.org/mediawiki/2021/ | + | <img style="width:810px;height: 345px;margin: 100px auto;display: block;" src="https://static.igem.org/mediawiki/2021/7/71/T--Shanghai_United--homepage07.jpg"> |
</div> | </div> | ||
</div> | </div> |
Latest revision as of 07:53, 18 October 2021
![](https://static.igem.org/mediawiki/2021/2/27/T--Shanghai_United--homepage01.png)
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Safety
Basic Safety Requirements
All personal items were stored in a separate meeting room and were not allowed to be brought into the laboratory, with the only exceptions being a notebook and a writing utensil.
Latex gloves, masks, and lab coats were required before entering the laboratory and must be worn when remaining in the area. Said types of equipment must be taken off and stored inside the laboratory before leaving the lab.
All used materials must be discarded in designated medical waste containers. Latex gloves and masks were changed timely.
All team members were expected to learn the evacuation routes of the building. In case of an emergency, members must be evacuated through these routes.
Equipment Precautions
Centrifuge:
The centrifuge must be balanced when used: when a test tube is being centrifuged at a specific slot, another test tube with approximately the same mass must be placed at a complimentary slot.
The metal lid of the centrifuge must be locked onto the rotation plate for protection in the event of a test tube being ejected at high velocity from the plate.
Alcohol burner:
A burner must be lit by matches or fire starters. A lit burner must not be used to light other burners.
A burner must be put out by its lid, and must not be put out through blowing or other means.
A burner is considered a fire and therefore must be handled with care.
An alcohol burner should not contain more than one-third of alcohol to prevent any fire risk.
Micropipettes:
Micropipettes that are purposed for RNA and DNA must be distinguished to avoid contamination.
Micropipettes must not be held upside-down; failure to do so may result in contamination and damage to the equipment.
Micropipette tips are single use only, and must not touch any potentially contaminated surfaces.
After using a micropipette, the equipment must be adjusted to its maximum volume to preserve the equipment.
Water bath:
Water baths must be used when filled with water. Failure to do so may result in fire hazards.
Other precautions
Due to the Covid-19 pandemic, all members were tested prior to entering the laboratory; only members with negative tests were allowed in.
Microbes Safety
In this project, we only use a safe and common lab strain, Escherichia coli DH5-α, and strictly follow the rules to conduct experiments under the supervision of our instructor.
![](https://static.igem.org/mediawiki/2021/7/71/T--Shanghai_United--homepage07.jpg)