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<div style="width:100%;position: relative;"> | <div style="width:100%;position: relative;"> | ||
<img style="width:100%;" src="https://static.igem.org/mediawiki/2021/9/95/T--Shanghai_United--homepage08.jpg"></img> | <img style="width:100%;" src="https://static.igem.org/mediawiki/2021/9/95/T--Shanghai_United--homepage08.jpg"></img> | ||
− | <p style="color:white;font-size:48px;position: absolute;font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:48px; font-weight:bold;display: block;bottom: 100px;left: 100px;">Model</p> | + | <p style="color:white;font-size:48px;position: absolute;font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:48px; font-weight:bold;display: block;bottom: 100px;left: 100px;">Model</p> |
</div> | </div> | ||
Line 219: | Line 219: | ||
<div> | <div> | ||
<p style="margin:0pt; orphans:0; text-align:justify; widows:0"><span | <p style="margin:0pt; orphans:0; text-align:justify; widows:0"><span | ||
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">In order to determine the effective concentration range | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:22px">In order to determine the effective concentration range |
− | of the arsenic (</span><span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">C</span><span | + | of the arsenic (</span><span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:22px">C</span><span |
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:7pt; vertical-align:sub">2</span><span | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:7pt; vertical-align:sub">2</span><span |
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">H</span><span | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:22px">H</span><span |
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:7pt; vertical-align:sub">6</span><span | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:7pt; vertical-align:sub">6</span><span |
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">AsNaO</span><span | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:22px">AsNaO</span><span |
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:7pt; vertical-align:sub">5</span><span | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:7pt; vertical-align:sub">5</span><span |
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">), we tested the </span><span | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:22px">), we tested the </span><span |
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">fluorescence intensity generated by a</span><span | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:22px">fluorescence intensity generated by a</span><span |
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">n</span><span | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:22px">n</span><span |
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px"> ARSD/amilGFP transformed </span><span | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:22px"> ARSD/amilGFP transformed </span><span |
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">E. coli</span><span | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:22px; font-style:italic">E. coli</span><span |
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px"> reacting for 1 hour in C</span><span | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:22px"> reacting for 1 hour in C</span><span |
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:7pt; vertical-align:sub">2</span><span | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:7pt; vertical-align:sub">2</span><span |
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">H</span><span | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:22px">H</span><span |
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:7pt; vertical-align:sub">6</span><span | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:7pt; vertical-align:sub">6</span><span |
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">AsNaO</span><span | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:22px">AsNaO</span><span |
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:7pt; vertical-align:sub">5</span><span | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:7pt; vertical-align:sub">5</span><span |
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px"> </span><span | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:22px"> </span><span |
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">solutions.</span><span | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:22px">solutions.</span><span |
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px"> </span></p> | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:22px"> </span></p> |
<p style="margin:0pt; orphans:0; text-align:justify; widows:0"><span | <p style="margin:0pt; orphans:0; text-align:justify; widows:0"><span | ||
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px"> </span></p> | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:22px"> </span></p> |
<p style="margin:0pt; orphans:0; text-align:justify; widows:0"><span | <p style="margin:0pt; orphans:0; text-align:justify; widows:0"><span | ||
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">The initial data is showing belo</span><span | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:22px">The initial data is showing belo</span><span |
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">w and we also used the value of the fluorescence | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:22px">w and we also used the value of the fluorescence |
intensity when the arsenic concentration is zero as the baseline for the primary elimination.</span></p> | intensity when the arsenic concentration is zero as the baseline for the primary elimination.</span></p> | ||
<p style="font-size:22px; line-height:150%; margin:20px auto; orphans:0; text-align:center; widows:0"><span | <p style="font-size:22px; line-height:150%; margin:20px auto; orphans:0; text-align:center; widows:0"><span | ||
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:16px">Table 1. Fluorescence intensity of E. | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:16px">Table 1. Fluorescence intensity of E. |
− | coli/</span><span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:16px">ArsD</span><span | + | coli/</span><span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:16px">ArsD</span><span |
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:9pt;">_amilGFP </span><span | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:9pt;">_amilGFP </span><span |
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:16px">in C</span><span | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:16px">in C</span><span |
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:7pt; ">2</span><span | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:7pt; ">2</span><span |
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:16px">H</span><span | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:16px">H</span><span |
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:7pt; ">6</span><span | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:7pt; ">6</span><span |
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:16px">AsNaO</span><span | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:16px">AsNaO</span><span |
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:7pt; ">5</span><span | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:7pt; ">5</span><span |
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:16px"> </span><span | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:16px"> </span><span |
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:16px">solutions</span></p> | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:16px">solutions</span></p> |
<img src="https://static.igem.org/mediawiki/2021/b/b6/T--Shanghai_United--model01.jpg" width="600" style="display: block;margin: 10px auto;"> | <img src="https://static.igem.org/mediawiki/2021/b/b6/T--Shanghai_United--model01.jpg" width="600" style="display: block;margin: 10px auto;"> | ||
<p style="margin:0pt; orphans:0; text-align:justify; widows:0"><span | <p style="margin:0pt; orphans:0; text-align:justify; widows:0"><span | ||
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px"> </span></p> | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:22px"> </span></p> |
<p style="margin:0pt; orphans:0; text-align:justify; widows:0"><span | <p style="margin:0pt; orphans:0; text-align:justify; widows:0"><span | ||
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:36px; font-weight:bold">Model I</span></p> | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:36px; font-weight:bold">Model I</span></p> |
<p style="margin:0pt; orphans:0; text-align:justify; widows:0"><span | <p style="margin:0pt; orphans:0; text-align:justify; widows:0"><span | ||
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">According to the scatter plots, there seemed some | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:22px">According to the scatter plots, there seemed some |
− | </span><span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">ups and downs which would cause difficulties | + | </span><span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:22px">ups and downs which would cause difficulties |
also less accuracy to build the model. We also thought to use the exponential equation or logarithmic equation | also less accuracy to build the model. We also thought to use the exponential equation or logarithmic equation | ||
to fit our data but the fitting degrees turn out quite low. Therefore, we chose to use </span><span | to fit our data but the fitting degrees turn out quite low. Therefore, we chose to use </span><span | ||
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">the </span><span | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:22px">the </span><span |
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">piecewise</span><span | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:22px">piecewise</span><span |
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px"> </span><span | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:22px"> </span><span |
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">H</span><span | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:22px">H</span><span |
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">ermite</span><span | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:22px">ermite</span><span |
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px"> </span><span | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:22px"> </span><span |
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">interpolation</span><span | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:22px">interpolation</span><span |
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px"> </span><span | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:22px"> </span><span |
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">method to build the model and finally drew the fitting | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:22px">method to build the model and finally drew the fitting |
curve.</span></p> | curve.</span></p> | ||
<p style="margin:0pt; orphans:0; text-align:justify; widows:0"><span | <p style="margin:0pt; orphans:0; text-align:justify; widows:0"><span | ||
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px"> </span></p> | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:22px"> </span></p> |
<p style="margin:0pt; orphans:0; text-align:justify; widows:0"><span | <p style="margin:0pt; orphans:0; text-align:justify; widows:0"><span | ||
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Coding we used is giving below:</span></p> | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:22px">Coding we used is giving below:</span></p> |
<p style="margin:0pt; orphans:0; text-align:justify; widows:0"><span | <p style="margin:0pt; orphans:0; text-align:justify; widows:0"><span | ||
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">“</span></p> | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:22px">“</span></p> |
<p style="margin:0pt; orphans:0; widows:0"><span | <p style="margin:0pt; orphans:0; widows:0"><span | ||
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">clear;clc;</span></p> | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:22px">clear;clc;</span></p> |
− | <p style="margin:0pt; orphans:0; widows:0"><span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">x0=[0 10 20 | + | <p style="margin:0pt; orphans:0; widows:0"><span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:22px">x0=[0 10 20 |
50 100 150];</span></p> | 50 100 150];</span></p> | ||
− | <p style="margin:0pt; orphans:0; widows:0"><span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">y0=[0 | + | <p style="margin:0pt; orphans:0; widows:0"><span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:22px">y0=[0 |
48345.77778 45807.44444 390431.2222 258369.1111 245812.5556];</span></p> | 48345.77778 45807.44444 390431.2222 258369.1111 245812.5556];</span></p> | ||
<p style="margin:0pt; orphans:0; widows:0"><span | <p style="margin:0pt; orphans:0; widows:0"><span | ||
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">x=[0:0.5:150];</span></p> | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:22px">x=[0:0.5:150];</span></p> |
<p style="margin:0pt; orphans:0; widows:0"><span | <p style="margin:0pt; orphans:0; widows:0"><span | ||
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">y=interp1(x0,y0,x</span><span | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:22px">y=interp1(x0,y0,x</span><span |
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">,</span><span | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:22px">,</span><span |
− | style="color:#a020f0; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">'pchip'</span><span | + | style="color:#a020f0; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:22px">'pchip'</span><span |
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">);</span></p> | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:22px">);</span></p> |
<p style="margin:0pt; orphans:0; widows:0"><span | <p style="margin:0pt; orphans:0; widows:0"><span | ||
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">ymax=max(y);</span></p> | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:22px">ymax=max(y);</span></p> |
<p style="margin:0pt; orphans:0; widows:0"><span | <p style="margin:0pt; orphans:0; widows:0"><span | ||
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">i=find(y==max(y))</span></p> | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:22px">i=find(y==max(y))</span></p> |
<p style="margin:0pt; orphans:0; widows:0"><span | <p style="margin:0pt; orphans:0; widows:0"><span | ||
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">xmax=x(i)</span></p> | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:22px">xmax=x(i)</span></p> |
<p style="margin:0pt; orphans:0; widows:0"><span | <p style="margin:0pt; orphans:0; widows:0"><span | ||
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">plot(x,y,</span><span | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:22px">plot(x,y,</span><span |
− | style="color:#a020f0; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">'LineWidth'</span><span | + | style="color:#a020f0; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:22px">'LineWidth'</span><span |
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">,2)</span></p> | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:22px">,2)</span></p> |
− | <p style="margin:0pt; orphans:0; widows:0"><span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">hold | + | <p style="margin:0pt; orphans:0; widows:0"><span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:22px">hold |
− | </span><span style="color:#a020f0; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">on</span><span | + | </span><span style="color:#a020f0; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:22px">on</span><span |
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px"> </span></p> | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:22px"> </span></p> |
<p style="margin:0pt; orphans:0; widows:0"><span | <p style="margin:0pt; orphans:0; widows:0"><span | ||
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">plot(xmax,max(y),</span><span | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:22px">plot(xmax,max(y),</span><span |
− | style="color:#a020f0; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">'r*'</span><span | + | style="color:#a020f0; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:22px">'r*'</span><span |
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">,</span><span | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:22px">,</span><span |
− | style="color:#a020f0; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">'LineWidth'</span><span | + | style="color:#a020f0; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:22px">'LineWidth'</span><span |
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">,2)</span></p> | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:22px">,2)</span></p> |
− | <p style="margin:0pt; orphans:0; widows:0"><span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">hold | + | <p style="margin:0pt; orphans:0; widows:0"><span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:22px">hold |
− | </span><span style="color:#a020f0; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">off</span></p> | + | </span><span style="color:#a020f0; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:22px">off</span></p> |
<p style="margin:0pt; orphans:0; text-align:justify; widows:0"><span | <p style="margin:0pt; orphans:0; text-align:justify; widows:0"><span | ||
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px"> </span></p> | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:22px"> </span></p> |
<p style="margin:0pt; orphans:0; text-align:justify; widows:0"><span | <p style="margin:0pt; orphans:0; text-align:justify; widows:0"><span | ||
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">”</span></p> | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:22px">”</span></p> |
<p style="margin:0pt; orphans:0; text-align:justify; widows:0"><img | <p style="margin:0pt; orphans:0; text-align:justify; widows:0"><img | ||
src="https://static.igem.org/mediawiki/2021/b/b0/T--Shanghai_United--model02.jpg" width="600" alt="" | src="https://static.igem.org/mediawiki/2021/b/b0/T--Shanghai_United--model02.jpg" width="600" alt="" | ||
Line 327: | Line 327: | ||
</p> | </p> | ||
<p style="font-size:22px; line-height:150%; margin:0pt; orphans:0; text-align:center; widows:0"><span | <p style="font-size:22px; line-height:150%; margin:0pt; orphans:0; text-align:center; widows:0"><span | ||
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:16px">Figure 1. </span><span | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:16px">Figure 1. </span><span |
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:16px">The f</span><span | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:16px">The f</span><span |
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:16px">itting curve of E. | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:16px">itting curve of E. |
coli/ArsD</span><span | coli/ArsD</span><span | ||
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:16px">_amilGFP</span><span | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:16px">_amilGFP</span><span |
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:16px"> by piecewise Hermite | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:16px"> by piecewise Hermite |
interpolation</span></p> | interpolation</span></p> | ||
<p style="margin:0pt; orphans:0; text-align:justify; widows:0"><span | <p style="margin:0pt; orphans:0; text-align:justify; widows:0"><span | ||
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">In figure 1, we noticed that the </span><span | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:22px">In figure 1, we noticed that the </span><span |
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">f</span><span | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:22px">f</span><span |
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">luorescence intensity</span><span | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:22px">luorescence intensity</span><span |
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px"> started to decline as the concentration of the arsenic | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:22px"> started to decline as the concentration of the arsenic |
increases after 50 ug/L. It is probably that the arsenic affects the growth of the engineered strain, the | increases after 50 ug/L. It is probably that the arsenic affects the growth of the engineered strain, the | ||
biosensor when the concentration exceeds 50ug/L. Therefore, we would ind</span><span | biosensor when the concentration exceeds 50ug/L. Therefore, we would ind</span><span | ||
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">icate the effective detection range of our biosensor (E. | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:22px">icate the effective detection range of our biosensor (E. |
coli/ArsD_amilGFP) to be 0~50 ug/L for the arsenic (</span><span | coli/ArsD_amilGFP) to be 0~50 ug/L for the arsenic (</span><span | ||
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">C</span><span | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:22px">C</span><span |
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:7pt; vertical-align:sub">2</span><span | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:7pt; vertical-align:sub">2</span><span |
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">H</span><span | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:22px">H</span><span |
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:7pt; vertical-align:sub">6</span><span | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:7pt; vertical-align:sub">6</span><span |
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">AsNaO</span><span | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:22px">AsNaO</span><span |
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:7pt; vertical-align:sub">5</span><span | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:7pt; vertical-align:sub">5</span><span |
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">).</span></p> | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:22px">).</span></p> |
<p style="margin:0pt; orphans:0; text-align:justify; widows:0"><span | <p style="margin:0pt; orphans:0; text-align:justify; widows:0"><span | ||
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px"> </span></p> | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:22px"> </span></p> |
<p style="font-size:12pt; line-height:150%; margin:0pt; orphans:0; text-align:justify; widows:0"><span | <p style="font-size:12pt; line-height:150%; margin:0pt; orphans:0; text-align:justify; widows:0"><span | ||
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:36px; font-weight:bold">Model II</span></p> | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:36px; font-weight:bold">Model II</span></p> |
<p style="font-size:22px; line-height:150%; margin:10px auto; orphans:0; text-align:justify; widows:0"><span | <p style="font-size:22px; line-height:150%; margin:10px auto; orphans:0; text-align:justify; widows:0"><span | ||
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">As we have determined the effective detection range of | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:22px">As we have determined the effective detection range of |
our arsenic biosensor, we thought to further analyze the relationship</span><span | our arsenic biosensor, we thought to further analyze the relationship</span><span | ||
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px"> between the </span><span | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:22px"> between the </span><span |
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">f</span><span | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:22px">f</span><span |
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">luorescence intensity</span><span | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:22px">luorescence intensity</span><span |
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px"> and the concentration of the arsenic.</span></p> | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:22px"> and the concentration of the arsenic.</span></p> |
<p style="font-size:22px; line-height:150%; margin:0pt; orphans:0; text-align:justify; widows:0"><span | <p style="font-size:22px; line-height:150%; margin:0pt; orphans:0; text-align:justify; widows:0"><span | ||
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Therefore, we only picked the data when the | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:22px">Therefore, we only picked the data when the |
concentration is not higher than 50 ug/L and adopted the following equation with the fitting degree, 1 by | concentration is not higher than 50 ug/L and adopted the following equation with the fitting degree, 1 by | ||
MATLAB:</span></p> | MATLAB:</span></p> | ||
Line 373: | Line 373: | ||
</p> | </p> | ||
<p style="font-size:22px; line-height:150%; margin:0pt; orphans:0; text-align:center; widows:0"><span | <p style="font-size:22px; line-height:150%; margin:0pt; orphans:0; text-align:center; widows:0"><span | ||
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:16px">Figure 2. Model result of </span><img | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:16px">Figure 2. Model result of </span><img |
src="https://static.igem.org/mediawiki/2021/8/81/T--Shanghai_United--model03.jpg" width="200" alt="" | src="https://static.igem.org/mediawiki/2021/8/81/T--Shanghai_United--model03.jpg" width="200" alt="" | ||
style="-aw-left-pos:0pt; -aw-rel-hpos:column; -aw-rel-vpos:paragraph; -aw-top-pos:0pt; -aw-wrap-type:inline" /> | style="-aw-left-pos:0pt; -aw-rel-hpos:column; -aw-rel-vpos:paragraph; -aw-top-pos:0pt; -aw-wrap-type:inline" /> | ||
</p> | </p> | ||
<p style="font-size:22px; line-height:150%; margin:0pt; orphans:0; text-align:center; widows:0"><span | <p style="font-size:22px; line-height:150%; margin:0pt; orphans:0; text-align:center; widows:0"><span | ||
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:16px"> </span></p> | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:16px"> </span></p> |
<p style="font-size:22px; line-height:150%; margin:0pt; orphans:0; text-align:center; widows:0"><img | <p style="font-size:22px; line-height:150%; margin:0pt; orphans:0; text-align:center; widows:0"><img | ||
src="https://static.igem.org/mediawiki/2021/c/ca/T--Shanghai_United--model06.jpg" width="500" alt="" | src="https://static.igem.org/mediawiki/2021/c/ca/T--Shanghai_United--model06.jpg" width="500" alt="" | ||
Line 384: | Line 384: | ||
</p> | </p> | ||
<p style="font-size:22px; line-height:150%; margin:0pt; orphans:0; text-align:center; widows:0"><span | <p style="font-size:22px; line-height:150%; margin:0pt; orphans:0; text-align:center; widows:0"><span | ||
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:16px">Figure 3. </span><span | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:16px">Figure 3. </span><span |
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:16px">The f</span><span | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:16px">The f</span><span |
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:16px">itting curve of </span><img | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:16px">itting curve of </span><img |
src="https://static.igem.org/mediawiki/2021/8/81/T--Shanghai_United--model03.jpg" width="200" alt="" | src="https://static.igem.org/mediawiki/2021/8/81/T--Shanghai_United--model03.jpg" width="200" alt="" | ||
style="-aw-left-pos:0pt; -aw-rel-hpos:column; -aw-rel-vpos:paragraph; -aw-top-pos:0pt; -aw-wrap-type:inline" /> | style="-aw-left-pos:0pt; -aw-rel-hpos:column; -aw-rel-vpos:paragraph; -aw-top-pos:0pt; -aw-wrap-type:inline" /> | ||
</p> | </p> | ||
<p style="font-size:22px; line-height:150%; margin:0pt; orphans:0; text-align:justify; widows:0"><span | <p style="font-size:22px; line-height:150%; margin:0pt; orphans:0; text-align:justify; widows:0"><span | ||
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">According to the model result of the cubic polynomial | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:22px">According to the model result of the cubic polynomial |
equation, it could establish the direct quantitative relationship between arsenic co</span><span | equation, it could establish the direct quantitative relationship between arsenic co</span><span | ||
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">ncentration and fluorescence intensity, which means we | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:22px">ncentration and fluorescence intensity, which means we |
could read the fluorescence intensity to get the value of the arsenic amount. Hence, this model could be used | could read the fluorescence intensity to get the value of the arsenic amount. Hence, this model could be used | ||
for the numerical display screen set up in the future to facilitate the application and enh</span><span | for the numerical display screen set up in the future to facilitate the application and enh</span><span | ||
− | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">ance the functionality.</span></p> | + | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;line-height: 200%; font-size:22px">ance the functionality.</span></p> |
</div> | </div> | ||
</div> | </div> |
Revision as of 08:35, 13 October 2021
![](https://static.igem.org/mediawiki/2021/2/27/T--Shanghai_United--homepage01.png)
![](https://static.igem.org/mediawiki/2021/9/95/T--Shanghai_United--homepage08.jpg)
Model
In order to determine the effective concentration range of the arsenic (C2H6AsNaO5), we tested the fluorescence intensity generated by an ARSD/amilGFP transformed E. coli reacting for 1 hour in C2H6AsNaO5 solutions.
The initial data is showing below and we also used the value of the fluorescence intensity when the arsenic concentration is zero as the baseline for the primary elimination.
Table 1. Fluorescence intensity of E. coli/ArsD_amilGFP in C2H6AsNaO5 solutions
![](https://static.igem.org/mediawiki/2021/b/b6/T--Shanghai_United--model01.jpg)
Model I
According to the scatter plots, there seemed some ups and downs which would cause difficulties also less accuracy to build the model. We also thought to use the exponential equation or logarithmic equation to fit our data but the fitting degrees turn out quite low. Therefore, we chose to use the piecewise Hermite interpolation method to build the model and finally drew the fitting curve.
Coding we used is giving below:
“
clear;clc;
x0=[0 10 20 50 100 150];
y0=[0 48345.77778 45807.44444 390431.2222 258369.1111 245812.5556];
x=[0:0.5:150];
y=interp1(x0,y0,x,'pchip');
ymax=max(y);
i=find(y==max(y))
xmax=x(i)
plot(x,y,'LineWidth',2)
hold on
plot(xmax,max(y),'r*','LineWidth',2)
hold off
”
Figure 1. The fitting curve of E. coli/ArsD_amilGFP by piecewise Hermite interpolation
In figure 1, we noticed that the fluorescence intensity started to decline as the concentration of the arsenic increases after 50 ug/L. It is probably that the arsenic affects the growth of the engineered strain, the biosensor when the concentration exceeds 50ug/L. Therefore, we would indicate the effective detection range of our biosensor (E. coli/ArsD_amilGFP) to be 0~50 ug/L for the arsenic (C2H6AsNaO5).
Model II
As we have determined the effective detection range of our arsenic biosensor, we thought to further analyze the relationship between the fluorescence intensity and the concentration of the arsenic.
Therefore, we only picked the data when the concentration is not higher than 50 ug/L and adopted the following equation with the fitting degree, 1 by MATLAB:
Figure 2. Model result of
Figure 3. The fitting curve of
According to the model result of the cubic polynomial equation, it could establish the direct quantitative relationship between arsenic concentration and fluorescence intensity, which means we could read the fluorescence intensity to get the value of the arsenic amount. Hence, this model could be used for the numerical display screen set up in the future to facilitate the application and enhance the functionality.
![](https://static.igem.org/mediawiki/2021/7/71/T--Shanghai_United--homepage07.jpg)