Difference between revisions of "Team:Shanghai HS ID/Notebook"

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{{IGEM_TopBar}}
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{{Shanghai_HS_ID}}
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<div class="column full_size">
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<head>
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    <meta charset="UTF-8">
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    <meta http-equiv="X-UA-Compatible" content="IE=edge">
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    <meta name="viewport" content="width=device-width, initial-scale=1.0">
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    <title>Shanghai_HS_ID</title>
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    <link rel="stylesheet"
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        href="https://2021.igem.org/wiki/index.php?title=Template:Shanghai_HS_ID/Main_CSS&action=raw&ctype=text/css" />
  
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<h1>Notebook</h1>
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</head>
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<p> Document the dates you worked on your project. This should be a detailed account of the work done each day for your project.</p>
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</div>
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<body>
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<div class="clear"></div>
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    <nav class="head-nav clearfix">
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        <div class="top-block"></div>
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        <div class="top-nav-bar">
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            <ul class="clearfix">
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                <span class="small-logo"></span>
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                <li>
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                    <a href="https://2021.igem.org/Team:Shanghai_HS_ID">Home</a>
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                </li>
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                <li class="active">
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                    <a href="">Project</a>
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                    <div class="sub-nav">
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                        <ul>
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                            <li><a href="https://2021.igem.org/Team:Shanghai_HS_ID/Description"
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                                    class="sub-nav-74">Description</a></li>
 +
                            <li><a href="https://2021.igem.org/Team:Shanghai_HS_ID/Experiments"
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                                    class="sub-nav-74">Experiments</a></li>
 +
                            <li><a href="https://2021.igem.org/Team:Shanghai_HS_ID/Results"
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                                    class="sub-nav-74">Results</a></li>
 +
                            <li><a href="https://2021.igem.org/Team:Shanghai_HS_ID/Proof_Of_Concept"
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                                    class="sub-nav-52">Proof Of Concept</a></li>
 +
                            <li class="current-sub-nav"><a href="#" class="sub-nav-52">Notebook</a></li>
 +
                            <li><a href="https://2021.igem.org/Team:Shanghai_HS_ID/Safety">Safety</a></li>
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                        </ul>
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                    </div>
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                </li>
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                <li>
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                    <a href="">Parts</a>
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                    <div class="sub-nav">
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                        <ul>
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                            <li><a href="https://2021.igem.org/Team:Shanghai_HS_ID/Collection" class="sub-nav-74">Parts
 +
                                    Collection</a></li>
 +
                            <li><a href="https://2021.igem.org/Team:Shanghai_HS_ID/Engineering"
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                                    class="sub-nav-74">Engineering</a></li>
 +
                            <li><a href="https://2021.igem.org/Team:Shanghai_HS_ID/Contribution"
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                                    class="sub-nav-74">Contribution</a></li>
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                        </ul>
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                    </div>
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                </li>
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                <li>
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                    <a href="">Human Practices</a>
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                    <div class="sub-nav">
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                        <ul>
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                            <li><a href="https://2021.igem.org/Team:Shanghai_HS_ID/Human_Practices"
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                                    class="sub-nav-74">Integrated Human Practice</a></li>
 +
                            <li><a href="https://2021.igem.org/Team:Shanghai_HS_ID/Communication"
 +
                                    class="sub-nav-74">Communication</a></li>
 +
                            <li><a href="https://2021.igem.org/Team:Shanghai_HS_ID/Fundraising"
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                                    class="sub-nav-74">Fundraising</a></li>
 +
                        </ul>
 +
                    </div>
 +
                </li>
 +
                <li>
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                    <a href="https://2021.igem.org/Team:Shanghai_HS_ID/Implementation">Implementation</a>
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                </li>
 +
                <li>
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                    <a href="https://2021.igem.org/Team:Shanghai_HS_ID/Entrepreneurship">Entrepreneurship</a>
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                </li>
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                <li>
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                    <a href="https://2021.igem.org/Team:Shanghai_HS_ID/Model">Model</a>
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                </li>
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                <li>
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                    <a href="">Team</a>
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                    <div class="sub-nav">
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                        <ul>
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                            <li><a href="https://2021.igem.org/Team:Shanghai_HS_ID/Members" class="sub-nav-74">Team
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                                    Members</a></li>
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                            <li><a href="https://2021.igem.org/Team:Shanghai_HS_ID/Attributions"
 +
                                    class="sub-nav-74">Attributions</a></li>
 +
                            <li><a href="https://2021.igem.org/Team:Shanghai_HS_ID/Collaborations"
 +
                                    class="sub-nav-74">Collaborations</a></li>
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                            <li><a href="https://2021.igem.org/Team:Shanghai_HS_ID/Partnership"
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                                    class="sub-nav-74">Partnership</a></li>
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                        </ul>
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                    </div>
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                </li>
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            </ul>
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        </div>
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    </nav>
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    <div class="sub-banner">
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        <img src="https://static.igem.org/mediawiki/2021/2/23/T--Shanghai_City_United--Description01.png" alt="" />
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    </div>
 +
    <div class="sub-content">
 +
        <div class="sub-title">NOTEBOOK</div>
 +
        <div class="article-content">27<sup>th</sup> June: We had our first team meeting and discussed our topic</div>
 +
        <div class="article-content">28<sup>th</sup> June: We made the work distribution and finalized the designs of
 +
            the team name, logo, and merch. Besides, we conducted the interview with an expert.</div>
 +
        <div class="img-wrap no-margin">
 +
            <img src="https://static.igem.org/mediawiki/2021/5/5f/T--Shanghai_HS_ID--Notebook01.jpg" alt="" />
 +
        </div>
 +
        <div class="article-content"><b>Wet team agenda</b></div>
 +
        <div class="article-content">28<sup>th</sup> June:</div>
 +
        <div class="article-content">• Lab safety training</div>
 +
        <div class="img-wrap no-margin">
 +
            <img src="https://static.igem.org/mediawiki/2021/2/2a/T--Shanghai_HS_ID--Notebook03.jpg" alt="" />
 +
        </div>
 +
        <div class="article-content">30<sup>th</sup> June- 2<sup>nd</sup> July:</div>
 +
        <div class="article-content">
 +
            • Construction of sgRNA, upstream and downstream homologous arms <br />
 +
            • Overlap PCR and electrophoresis<br />
 +
            • Preparation of mediums<br />
 +
            • Bacteria culture
 +
        </div>
 +
        <div class="img-wrap no-margin">
 +
            <img src="https://static.igem.org/mediawiki/2021/a/a9/T--Shanghai_HS_ID--Notebook04.jpg" alt="" />
 +
        </div>
 +
        <div class="article-content">3<sup>rd</sup> July:</div>
 +
        <div class="article-content">
 +
            • pLCNICK enzyme digestion <br />
 +
            • Connection of sgRNA and pLCNICK<br />
 +
            • Transformation of the recombinant plasmid into E.coli
 +
        </div>
 +
        <div class="img-wrap no-margin">
 +
            <img src="https://static.igem.org/mediawiki/2021/6/66/T--Shanghai_HS_ID--Notebook05.jpg" alt="" />
 +
        </div>
 +
        <div class="article-content">4<sup>th</sup> July:</div>
 +
        <div class="article-content">
 +
            • Culture L. casei cells<br />
 +
            • Preparation of competent L. casei cells
 +
        </div>
 +
        <div class="img-wrap no-margin">
 +
            <img src="https://static.igem.org/mediawiki/2021/2/2a/T--Shanghai_HS_ID--Notebook06.jpg" alt="" />
 +
        </div>
 +
        <div class="article-content">5<sup>th</sup> July:</div>
 +
        <div class="article-content">
 +
            • Picked and shakes L. casei<br />
 +
            • L. Casei PCR and electrophoresis
 +
        </div>
 +
        <div class="img-wrap no-margin">
 +
            <img src="https://static.igem.org/mediawiki/2021/8/8d/T--Shanghai_HS_ID--Notebook08.jpg" alt="" />
 +
        </div>
 +
        <div class="article-content">6<sup>th</sup>-7<sup>th</sup> July : </div>
 +
        <div class="article-content">
 +
            • Verification of the plasmid<br />
 +
            • Electrotransformation of the plasmid into L. casei ATCC 533
 +
            August an September<br />
 +
            • Repeat the plamid construction and electrotransformation<br />
 +
            • L. casei cultivation
  
−
 
+
        </div>
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+
        <div class="img-wrap no-margin">
−
<div class="column two_thirds_size">
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            <img src="https://static.igem.org/mediawiki/2021/7/74/T--Shanghai_HS_ID--Notebook09.jpg" alt="" />
−
<h3>What should this page have?</h3>
+
        </div>
−
<ul>
+
        <div class="article-title">Dry team agenda </div>
−
<li>Chronological notes of what your team is doing.</li>
+
        <div class="article-content">28<sup>th</sup> June: </div>
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<li> Brief descriptions of daily important events.</li>
+
        <div class="article-content">• Published the first article of our Wechat official account: introducing us and
−
<li>Pictures of your progress. </li>
+
            our topic.</div>
−
<li>Mention who participated in what task.</li>
+
        <div class="img-wrap no-margin">
−
</ul>
+
            <img src="https://static.igem.org/mediawiki/2021/d/d9/T--Shanghai_HS_ID--Notebook10.jpg" alt="" />
−
 
+
        </div>
−
</div>
+
        <div class="article-content">30<sup>th</sup> June:</div>
−
 
+
        <div class="article-content">
−
<div class="column third_size">
+
            • Discussed the script of our promotion video <br />
−
<div class="highlight decoration_A_full">
+
            • Listed survey questions and interview questions
−
<h3>Inspiration</h3>
+
        </div>
−
<p>You can see what others teams have done to organize their notes:</p>
+
        <div class="img-wrap no-margin">
−
 
+
            <img src="https://static.igem.org/mediawiki/2021/f/f4/T--Shanghai_HS_ID--Notebook11.jpg" alt="" />
−
<ul>  
+
        </div>
−
<li><a href="https://2018.igem.org/Team:Munich/Notebook">2018 Munich</a></li>
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        <div class="article-content">1<sup>st</sup> July : </div>
−
 
+
        <div class="article-content">
−
<li><a href="https://2019.igem.org/Team:Georgia_State/Notebook">2019 Georgia State</a></li>
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            • Organized and analyzed the records of the 1st Expert Interview<br />
−
<li><a href="https://2019.igem.org/Team:Newcastle/Notebook">2019 Newcastle</a></li>
+
            • Gathered information for our topic background<br />
−
 
+
            • Prepared a question list for the site visit to Bright Dairy & Food company in the next day<br />
−
 
+
            • Assigned tasks for the next day's site visit
−
<li><a href="https://2020.igem.org/Team:IISER-Pune-India/Notebook">2020 IISER Pune India</a></li>
+
        </div>
−
<li><a href="https://2020.igem.org/Team:Lund/Notebook">2020 Lund</a></li>
+
        <div class="article-content">2<sup>nd</sup> July : </div>
−
<li><a href="https://2020.igem.org/Team:NOVA_LxPortugal/Notebook">2020 NOVA LxPortugal</a></li>
+
        <div class="article-content">
−
<li><a href="https://2020.igem.org/Team:RDFZ-China/NoteBook">2020 RDFZ China</a></li>
+
            • Data analysis on survey results<br />
−
</ul>
+
            • Integrated all interview records of the past few days<br />
−
</div>
+
            • Prepared questions for the 2nd Expert Interview<br />
−
</div>
+
            • Drafted a new article of our public Wechat account for the site visit<br />
 +
            • Ordered merch: notebooks<br />
 +
            • Designed post-it notes<br />
 +
            • organize stuff for the article for the merch sale
 +
        </div>
 +
        <div class="img-wrap no-margin">
 +
            <img src="https://static.igem.org/mediawiki/2021/f/f4/T--Shanghai_HS_ID--Notebook12.jpg" alt="" />
 +
        </div>
 +
        <div class="article-content">3<sup>rd</sup> July : </div>
 +
        <div class="article-content">
 +
            • Drafted self-introductions<br />
 +
            • Drafted integrated human practices (transcription and translation of interview records, data of our Wechat
 +
            official account and survey results)<br />
 +
            • Booked another interview<br />
 +
            • Listed interview questions<br />
 +
            • Publish the second article of our Wechat official account, which was focused on our site visit to Bright
 +
            Dairy & Food company.
 +
        </div>
 +
        <div class="article-content">4<sup>th</sup> July : </div>
 +
        <div class="article-content">
 +
            • Drafted inspiration<br />
 +
            • Discussed to determine the lecture location and target audience<br />
 +
            • Promotion video filming<br />
 +
            • Roll Screen design<br />
 +
            • Visit BFC for merch-sale site pre-check
 +
        </div>
 +
        <div class="img-wrap no-margin">
 +
            <img src="https://static.igem.org/mediawiki/2021/4/4a/T--Shanghai_HS_ID--Notebook13.jpg" alt="" />
 +
        </div>
 +
        <div class="article-content">5<sup>th</sup> July : </div>
 +
        <div class="article-content">
 +
            • Finalized IHP and inspiration<br />
 +
            • Finalized details and publicize charity sale<br />
 +
            • Started writing implementation and business plan<br />
 +
            • Promotion video filming
 +
        </div>
 +
        <div class="img-wrap no-margin">
 +
            <img src="https://static.igem.org/mediawiki/2021/f/f0/T--Shanghai_HS_ID--Notebook14.jpg" alt="" />
 +
        </div>
 +
        <div class="article-content">6<sup>th</sup> July : </div>
 +
        <div class="article-content">
 +
            • Drafted attributions, business plan<br />
 +
            • Initially completed the script of our promotion video
 +
        </div>
 +
        <div class="img-wrap no-margin">
 +
            <img src="https://static.igem.org/mediawiki/2021/5/5b/T--Shanghai_HS_ID--Notebook15.jpg" alt="" />
 +
        </div>
 +
        <div class="article-content">7<sup>th</sup> July : </div>
 +
        <div class="article-content">
 +
            • Planed for another charity sale<br />
 +
            • Started writing a new article for merch sale to be published in our Wechat official account<br />
 +
            • Collaboration activity<br />
 +
            • Built our cost structure<br />
 +
            • Finalized the script for promotion video<br />
 +
            • First merch sale: yielded a profit of 50 RMB around and got 13 more followers
 +
        </div>
 +
        <div class="img-wrap no-margin">
 +
            <img src="https://static.igem.org/mediawiki/2021/c/cd/T--Shanghai_HS_ID--Notebook16.jpg" alt="" />
 +
        </div>
 +
        <div class="article-content">8<sup>th</sup> July : </div>
 +
        <div class="article-content">
 +
            • Drafted future plan, safety, public engagement, and education<br />
 +
            • Organized what we have done and drafted the first version of the presentation PowerPoint<br />
 +
            • Fundraising
 +
        </div>
 +
        <div class="img-wrap no-margin">
 +
            <img src="https://static.igem.org/mediawiki/2021/c/c9/T--Shanghai_HS_ID--Notebook17.jpg" alt="" />
 +
        </div>
 +
        <div class="article-content">11<sup>th</sup> July : </div>
 +
        <div class="article-content">
 +
            • Published the article about our merch sale in Wechat official account<br />
 +
            • Determine the sale day online for our merch
 +
        </div>
 +
    </div>
 +
    <footer class="footer">
 +
        <section class="footer-wrap">
 +
            <div class="footer-contact">Follow us on WeChat!</div>
 +
            <img class="footer-qrcode" src="https://static.igem.org/mediawiki/2021/c/c3/T--Shanghai_HS_ID--qrcode.png" />
 +
            <p class="margin-bottom-10"><i>WeChat Official Account: 乳酸LAB</i></p>
 +
            <p>Contact Email：<i>jhxchen@gmail.com</i></p>
 +
        </section>
 +
    </footer>
 +
</body>
 +
<script>
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Revision as of 18:02, 11 October 2021

Shanghai_HS_ID

NOTEBOOK
27th June: We had our first team meeting and discussed our topic
28th June: We made the work distribution and finalized the designs of the team name, logo, and merch. Besides, we conducted the interview with an expert.
Wet team agenda
28th June:
• Lab safety training
30th June- 2nd July:
• Construction of sgRNA, upstream and downstream homologous arms
• Overlap PCR and electrophoresis
• Preparation of mediums
• Bacteria culture
3rd July:
• pLCNICK enzyme digestion
• Connection of sgRNA and pLCNICK
• Transformation of the recombinant plasmid into E.coli
4th July:
• Culture L. casei cells
• Preparation of competent L. casei cells
5th July:
• Picked and shakes L. casei
• L. Casei PCR and electrophoresis
6th-7th July :
• Verification of the plasmid
• Electrotransformation of the plasmid into L. casei ATCC 533 August an September
• Repeat the plamid construction and electrotransformation
• L. casei cultivation
Dry team agenda
28th June:
• Published the first article of our Wechat official account: introducing us and our topic.
30th June:
• Discussed the script of our promotion video
• Listed survey questions and interview questions
1st July :
• Organized and analyzed the records of the 1st Expert Interview
• Gathered information for our topic background
• Prepared a question list for the site visit to Bright Dairy & Food company in the next day
• Assigned tasks for the next day's site visit
2nd July :
• Data analysis on survey results
• Integrated all interview records of the past few days
• Prepared questions for the 2nd Expert Interview
• Drafted a new article of our public Wechat account for the site visit
• Ordered merch: notebooks
• Designed post-it notes
• organize stuff for the article for the merch sale
3rd July :
• Drafted self-introductions
• Drafted integrated human practices (transcription and translation of interview records, data of our Wechat official account and survey results)
• Booked another interview
• Listed interview questions
• Publish the second article of our Wechat official account, which was focused on our site visit to Bright Dairy & Food company.
4th July :
• Drafted inspiration
• Discussed to determine the lecture location and target audience
• Promotion video filming
• Roll Screen design
• Visit BFC for merch-sale site pre-check
5th July :
• Finalized IHP and inspiration
• Finalized details and publicize charity sale
• Started writing implementation and business plan
• Promotion video filming
6th July :
• Drafted attributions, business plan
• Initially completed the script of our promotion video
7th July :
• Planed for another charity sale
• Started writing a new article for merch sale to be published in our Wechat official account
• Collaboration activity
• Built our cost structure
• Finalized the script for promotion video
• First merch sale: yielded a profit of 50 RMB around and got 13 more followers
8th July :
• Drafted future plan, safety, public engagement, and education
• Organized what we have done and drafted the first version of the presentation PowerPoint
• Fundraising
11th July :
• Published the article about our merch sale in Wechat official account
• Determine the sale day online for our merch
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WeChat Official Account: 乳酸LAB

Contact Email：jhxchen@gmail.com