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<title>Document</title> | <title>Document</title> | ||
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+ | href="https://2021.igem.org/Team:Shanghai_United/Template:CSS?action=raw&ctype=text/css"> | ||
+ | <link rel="stylesheet" href="//unpkg.com/layui@2.6.8/dist/css/layui.css"> | ||
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+ | <script src="https://code.jquery.com/jquery-3.1.1.min.js"></script> | ||
+ | <script src="https://stackpath.bootstrapcdn.com/bootstrap/3.4.1/js/bootstrap.min.js" | ||
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style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">on the</span><span | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">on the</span><span | ||
style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">bottom of</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">the</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">tube</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">with a</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">micropipette.</span></p> | ||
+ | <p style="margin:3pt 0pt 0pt 30pt; orphans:0; text-indent:-21pt; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">9.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">        </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">In</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">a</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">sterile</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">environment,</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">transfer</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">the</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">transformed bacteria</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">to</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">prepared agar</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">plates.</span></p> | ||
+ | <p style="margin:0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:13pt"> </span></p> | ||
+ | <p style="margin:0.5pt 0pt 0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:11pt"> </span></p> | ||
+ | <h1 style="margin:0pt 0pt 0pt 9pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:36px; font-weight:bold;">Colony PCR Identification</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-weight:bold; letter-spacing:-0.3pt;"> | ||
+ | </span> | ||
+ | </h1> | ||
+ | <p style="margin:7.2pt 0pt 0pt 30pt; orphans:0; text-indent:-21pt; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.05pt">A.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">Materials</span></p> | ||
+ | <p style="margin:0.2pt 0pt 0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:6pt; font-style:italic"> </span></p> | ||
+ | <table cellspacing="0" cellpadding="0" style="border-collapse:collapse; margin-left:6.5pt"> | ||
+ | <tr style="height:36pt"> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:8.9pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">2x PCR</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">mix</span></p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:8.9pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">10 μL</span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style="height:36pt"> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:9pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Forward</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">primer</span></p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:9pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">0.5 μL</span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style="height:36pt"> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:8.95pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Reverse</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">primer</span></p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:8.95pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">0.5 μL</span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style="height:36pt"> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:8.9pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Template</span></p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:8.9pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Desired </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">E.</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.1pt"> | ||
+ | </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">Coli</span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style="height:36pt"> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:8.85pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">ddH</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:7.5pt">2</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">O</span></p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:9pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">9 μL</span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | </table> | ||
+ | <p style="margin:2.95pt 0pt 0pt 28.2pt; orphans:0; text-indent:-19.2pt; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.05pt">B.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">      </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">Protocol</span></p> | ||
+ | <p style="margin:7.05pt 0pt 0pt 46.4pt; orphans:0; text-indent:-19.25pt; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">1.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Refer</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">to</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">PCR</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.2pt"> | ||
+ | </span><span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">of</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.05pt"> | ||
+ | </span><span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">amilGFP</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.2pt"> | ||
+ | </span><span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">Gene, Jul.</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.05pt"> | ||
+ | </span><span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">28,</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.05pt"> | ||
+ | </span><span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">2021</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">.</span></p> | ||
+ | <p style="margin:7.2pt 0pt 0pt 46.4pt; orphans:0; text-indent:-19.25pt; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">2.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Notes</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">to</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">this</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">reaction:</span></p> | ||
+ | <p | ||
+ | style="font-size:22px; line-height:130%; margin:7.25pt 50.3pt 0pt 67.4pt; orphans:0; text-indent:-19.2pt; widows:0"> | ||
+ | <span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal; letter-spacing:-0.05pt">a.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Primers</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">were</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">delivered</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">in</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">the</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">form</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">of</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">dry</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">powder.</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.3pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Centrifuge</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">primers</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">at</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-2.85pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">12000</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">rpm</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">for</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">5</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">minutes to</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">deposit powder at the</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">bottom</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">of its</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">tube.</span></p> | ||
+ | <p style="margin:2.85pt 0pt 0pt 67.4pt; orphans:0; text-indent:-19.25pt; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal; letter-spacing:-0.05pt">b.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Dilute</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">primers</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">to</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">a concentration</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">of</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">10</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">nmol/ml.</span></p> | ||
+ | <p style="margin:7.2pt 0pt 0pt 67.4pt; orphans:0; text-indent:-19.25pt; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal; letter-spacing:-0.05pt">c.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Mix</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">the</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">2x</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">PCR</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.25pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">mix,</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.25pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">R.primer,</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.55pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Templalate,</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">ddH2O</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">as</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">master</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">mix.</span></p> | ||
+ | <p style="margin:7.2pt 0pt 0pt 28.2pt; orphans:0; text-indent:-19.2pt; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.05pt">C.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">      </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">Reacting</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.1pt"> | ||
+ | </span><span style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">conditions</span></p> | ||
+ | <p style="margin:0.2pt 0pt 0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:6pt; font-style:italic"> </span></p> | ||
+ | <table cellspacing="0" cellpadding="0" style="border-collapse:collapse; margin-left:6.5pt"> | ||
+ | <tr style="height:36pt"> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:149.75pt"> | ||
+ | <p style="margin:8.95pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">98 °C</span></p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:149.75pt"> | ||
+ | <p style="margin:8.95pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">10</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">minutes</span></p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:149.75pt"> | ||
+ | <p style="margin:8.95pt 0pt 0pt 2.9pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">1</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">cycle</span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style="height:36pt"> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:149.75pt"> | ||
+ | <p style="margin:9pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">98 °C</span></p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:149.75pt"> | ||
+ | <p style="margin:9pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">15</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">seconds</span></p> | ||
+ | </td> | ||
+ | <td rowspan="3" | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:149.75pt"> | ||
+ | <p style="margin:0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:13pt; font-style:italic"> </span></p> | ||
+ | <p style="margin:0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:13pt; font-style:italic"> </span></p> | ||
+ | <p style="margin:0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:14pt; font-style:italic"> </span></p> | ||
+ | <p style="margin:0pt 0pt 0pt 2.9pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">26</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">cycles</span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style="height:36pt"> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:149.75pt"> | ||
+ | <p style="margin:9pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">55 °C</span></p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:149.75pt"> | ||
+ | <p style="margin:9pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">15</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">seconds</span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style="height:35.9pt"> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:149.75pt"> | ||
+ | <p style="margin:8.95pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">72 °C</span></p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:149.75pt"> | ||
+ | <p style="margin:8.95pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">30</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">seconds</span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | </table> | ||
+ | </div><br style="clear:both; mso-break-type:section-break; page-break-before:always" /> | ||
+ | <div> | ||
+ | <p style="margin:0.5pt 0pt 0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:3pt; font-style:italic"> </span></p> | ||
+ | <table cellspacing="0" cellpadding="0" style="border-collapse:collapse; margin-left:6.5pt"> | ||
+ | <tr style="height:45pt"> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:149.75pt"> | ||
+ | <p style="margin:0.3pt 0pt 0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:11.5pt; font-style:italic"> </span></p> | ||
+ | <p style="margin:0pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">72 °C</span></p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:149.75pt"> | ||
+ | <p style="margin:0.3pt 0pt 0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:11.5pt; font-style:italic"> </span></p> | ||
+ | <p style="margin:0pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">5</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">minutes</span></p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:149.75pt"> | ||
+ | <p style="margin:0.3pt 0pt 0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:11.5pt; font-style:italic"> </span></p> | ||
+ | <p style="margin:0pt 0pt 0pt 2.9pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">1</span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> </span><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">cycle</span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style="height:36pt"> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:149.75pt"> | ||
+ | <p style="margin:9.05pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">4 °C</span></p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:149.75pt"> | ||
+ | <p style="margin:9.05pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Infinite</span></p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:149.75pt"> | ||
+ | <p style="margin:9.05pt 0pt 0pt 2.9pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">N/A</span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | </table> | ||
+ | <p style="margin:0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:11pt"> </span></p> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div id="myModal2" class="reveal-modal"> | ||
+ | <div style="overflow:scroll;height: 700px;width:1000px"> | ||
+ | <div> | ||
+ | <h1 style="margin:7pt 0pt 0pt 9pt; orphans:0; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:36px; font-weight:bold;">LB Culture Medium Preperation</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-weight:bold; letter-spacing:-0.3pt;"> | ||
+ | </span> | ||
+ | </h1> | ||
+ | <ol type="A" style="margin:0pt; padding-left:0pt"> | ||
+ | <li | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.05pt; margin:7.05pt 0pt 0pt 24.33pt; orphans:0; padding-left:3.87pt; text-indent:0pt; widows:0"> | ||
+ | <span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">Formula</span> | ||
+ | </li> | ||
+ | </ol> | ||
+ | <p style="margin:0.3pt 0pt 0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:6pt; font-style:italic"> </span></p> | ||
+ | <table cellspacing="0" cellpadding="0" style="border-collapse:collapse; margin-left:6.5pt"> | ||
+ | <tr style="height:36pt"> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:9pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Yeast</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.7pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">extract</span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:9pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">5</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">g/L</span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style="height:36pt"> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:8.95pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Tryptone</span></p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:8.95pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">10</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">g/L</span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style="height:36pt"> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:8.9pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">NaCl</span></p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:8.9pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">10</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">g/L</span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style="height:36pt"> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:9pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Agar*</span></p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:9pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">1.5%</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">of</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">total</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">mass</span> | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style="height:36pt"> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:8.9pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">ddH</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:7.5pt">2</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">O</span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:8.95pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Desired volume</span> | ||
+ | </p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | </table> | ||
+ | <p style="margin:2.9pt 0pt 0pt 9pt; orphans:0; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">*Agar</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">is</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">only</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">necessary</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">when LB</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">culture</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">medium</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">is</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">prepared</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">as</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">solid</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">agar</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">gel.</span></p> | ||
+ | <ol start="2" type="A" style="margin:0pt; padding-left:0pt"> | ||
+ | <li | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.05pt; margin:7.2pt 0pt 0pt 24.33pt; orphans:0; padding-left:3.87pt; text-indent:0pt; widows:0"> | ||
+ | <span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">Sterilization</span> | ||
+ | </li> | ||
+ | </ol> | ||
+ | <p style="margin:7.2pt 0pt 0pt 51.2pt; orphans:0; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Sterilize</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">at</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">121</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">°C</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">for</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">30 minutes.</span></p> | ||
+ | <ol start="3" type="A" style="margin:0pt; padding-left:0pt"> | ||
+ | <li | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt; margin:7.05pt 0pt 0pt 25pt; orphans:0; padding-left:3.2pt; text-indent:0pt; widows:0"> | ||
+ | <span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">KANA</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.35pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">antibiotics</span> | ||
+ | </li> | ||
+ | </ol> | ||
+ | <p style="font-size:22px; line-height:152%; margin:7.1pt 74.45pt 0pt 51.2pt; orphans:0; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Concentration</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">of</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">KANA</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.7pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">antibiotics</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">in</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">solution</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">is</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.25pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">50</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">mg/μL.</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-2.85pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Functional</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">concentration</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">of</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">KANA</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.7pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">antibiotics</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">is</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">50</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">μg/μL.</span></p> | ||
+ | <p style="margin:0.2pt 0pt 0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:18pt"> </span></p> | ||
+ | <p style="margin:0.05pt 0pt 0pt 9pt; orphans:0; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; font-weight:bold;">E. coli Cultivation</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; font-weight:bold; letter-spacing:-0.05pt;"> | ||
+ | </span> | ||
+ | <p style="margin:7.05pt 0pt 0pt 28.2pt; orphans:0; text-indent:-19.2pt; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.05pt">A.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">      </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">Protocol</span> | ||
+ | </p> | ||
+ | <p | ||
+ | style="font-size:22px; line-height:130%; margin:7.2pt 44.8pt 0pt 46.4pt; orphans:0; text-indent:-19.2pt; widows:0"> | ||
+ | <span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">1.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Clean workstation with 75% ethanol | ||
+ | and work near a lit alcohol lamp in steril</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-2.85pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">conditions.</span></p> | ||
+ | <p style="margin:3pt 0pt 0pt 46.4pt; orphans:0; text-indent:-19.25pt; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">2.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Prepare</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">LB</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">culture</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">medium</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">without</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">KANA</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.75pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">antibiotics.</span></p> | ||
+ | <p | ||
+ | style="font-size:22px; line-height:130%; margin:7.1pt 61.55pt 0pt 46.4pt; orphans:0; text-indent:-19.2pt; widows:0"> | ||
+ | <span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">3.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt">Prepare</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.05pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt">three</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.05pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt">lines</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px"> </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt">of</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px"> </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.05pt">E.</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic"> </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.05pt">coli</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic"> </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">with KANA</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.65pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">resistance</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.2pt"> </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">and</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.6pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">ARSA,</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.75pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">ARSD,</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">or</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-2.85pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">ARSR</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">plasmids depending on | ||
+ | the</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">cell</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.1pt"> </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">line.</span></p> | ||
+ | <p | ||
+ | style="font-size:22px; line-height:130%; margin:3pt 76.2pt 0pt 46.4pt; orphans:0; text-indent:-19.2pt; widows:0"> | ||
+ | <span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">4.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Add</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">KANA</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.7pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">antibiotics</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">to</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">LB</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">culture</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">medium</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">in</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">a</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">ratio</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">of 1</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">μL</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.45pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">KANA</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-2.85pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">antibiotics</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">: 1 mL</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.5pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">LB</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.15pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">culture</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">medium.</span></p> | ||
+ | <p | ||
+ | style="font-size:22px; line-height:130%; margin:3pt 66.1pt 0pt 46.4pt; orphans:0; text-indent:-19.2pt; widows:0"> | ||
+ | <span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">5.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Prepare</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">three</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">marked 15</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">mL</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.55pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">centrifure</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">tubes.</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Distribute</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">4</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">mL</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.6pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">LB</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">culture</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-2.85pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">medium</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">to</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">each tube.</span></p> | ||
+ | <p style="margin:3pt 0pt 0pt 46.4pt; orphans:0; text-indent:-19.25pt; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">6.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Transfer</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">10</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">μL</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.6pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">of</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">bacterial</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">solution</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">to</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.25pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">its</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">corresponding</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">tube.</span></p> | ||
+ | <p style="margin:7.2pt 0pt 0pt 46.4pt; orphans:0; text-indent:-19.25pt; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">7.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Cultivate</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.25pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">in a shaker</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">in</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">37 °C</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">at 220</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">rpm.</span></p> | ||
+ | </div><br style="clear:both; mso-break-type:section-break; page-break-before:always" /> | ||
+ | <div> | ||
+ | <h1 style="margin:4pt 0pt 0pt 9pt; orphans:0; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-weight:bold; letter-spacing:-0.05pt;">Preparation of Agar Plates</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-weight:bold;"> | ||
+ | </span> | ||
+ | </h1> | ||
+ | <p style="margin:7.05pt 0pt 0pt 28.2pt; orphans:0; text-indent:-19.2pt; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.05pt">A.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">      </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">Protocol</span> | ||
+ | </p> | ||
+ | <p | ||
+ | style="font-size:22px; line-height:130%; margin:7.1pt 43.8pt 0pt 46.4pt; orphans:0; text-indent:-19.2pt; widows:0"> | ||
+ | <span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">1.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Heat the pre-prepared solid LB | ||
+ | culture medium in a microwave until the solid</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-2.85pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">completely</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">melts.</span></p> | ||
+ | <p | ||
+ | style="font-size:22px; line-height:130%; margin:3.1pt 47.25pt 0pt 46.4pt; orphans:0; text-indent:-19.2pt; widows:0"> | ||
+ | <span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">2.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Add</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">KANA</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.7pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">antibiotics</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">in</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">a</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">ratio of</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">1</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">μL</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.5pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">KANA</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.7pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">antibiotics</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">:</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">1000</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">μL</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.55pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">culture</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-2.85pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">medium.</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Mix</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">the</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">solutions | ||
+ | throughly.</span></p> | ||
+ | <p | ||
+ | style="font-size:22px; line-height:130%; margin:2.9pt 71pt 0pt 46.4pt; orphans:0; text-indent:-19.2pt; widows:0"> | ||
+ | <span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">3.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">In a</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">sterile</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">environment,</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">carefully pour</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">approximately</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">20-30 mL</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.6pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">culture</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-2.85pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">medium</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">to</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">each empty plate.</span> | ||
+ | </p> | ||
+ | <p | ||
+ | style="font-size:22px; line-height:130%; margin:3pt 42.95pt 0pt 46.4pt; orphans:0; text-indent:-19.2pt; widows:0"> | ||
+ | <span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">4.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Tilt</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">the</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">plate</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">genly to</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">cover</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">the</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">plate</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">completely</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">with</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">culture</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">medium.</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Set</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">the</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-2.85pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">plates</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">still</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">and</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">wait for</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">culture medium | ||
+ | to</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">cool</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">and</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">solidify.</span></p> | ||
+ | <p style="margin:0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:13pt"> </span></p> | ||
+ | <h1 style="margin:9.05pt 0pt 0pt 9pt; orphans:0; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-weight:bold;">Preservation of Transformed E. coli</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-weight:bold; letter-spacing:-0.4pt;"> | ||
+ | </span> | ||
+ | </h1> | ||
+ | <p style="margin:7.1pt 0pt 0pt 30pt; orphans:0; text-indent:-21pt; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.05pt">A.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">Materials</span> | ||
+ | </p> | ||
+ | <p style="margin:0.3pt 0pt 0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:6pt; font-style:italic"> </span></p> | ||
+ | <table cellspacing="0" cellpadding="0" style="border-collapse:collapse; margin-left:6.5pt"> | ||
+ | <tr style="height:36pt"> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:8.95pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Glycerol</span></p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:8.95pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">900 µL</span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style="height:36pt"> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:9.05pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">LB</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">culture</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">medium</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">with</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">E.coli</span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:9.05pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">900 µL</span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | </table> | ||
+ | <p style="margin:3pt 0pt 0pt 30pt; orphans:0; text-indent:-21pt; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.05pt">B.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">Protocol</span> | ||
+ | </p> | ||
+ | <p | ||
+ | style="font-size:22px; line-height:130%; margin:7.05pt 47.85pt 0pt 49.15pt; orphans:0; text-indent:-19.2pt; widows:0"> | ||
+ | <span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">1.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Sterilize</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">work</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">station</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">and</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">work</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">near</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">a</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">lit</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">alcohol</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">burner.</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.25pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Prepare</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">and mark</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">2</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-2.85pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">mL</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.55pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">centrifuge</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">tubes.</span></p> | ||
+ | <p style="margin:3pt 0pt 0pt 49.2pt; orphans:0; text-indent:-19.2pt; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">2.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Add</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">900</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">µL</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.55pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">glycerol</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">to</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">a 2</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">mL</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.55pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">centrifuge tube.</span> | ||
+ | </p> | ||
+ | <p style="margin:7.2pt 0pt 0pt 49.2pt; orphans:0; text-indent:-19.2pt; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">3.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Add</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">900</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">µL</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.55pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">LB</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.1pt"> </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">culture</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">medium</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">with</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">E.coli</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">to</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">its</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">corresponding</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">tube.</span></p> | ||
+ | <p style="margin:7.1pt 0pt 0pt 49.2pt; orphans:0; text-indent:-19.2pt; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">4.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Apply</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">laboratory | ||
+ | film</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">to</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">seal every</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">centrifuge</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">tube.</span></p> | ||
+ | <p style="margin:7.2pt 0pt 0pt 49.2pt; orphans:0; text-indent:-19.2pt; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">5.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Store</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">at</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">-20˚C.</span></p> | ||
+ | <p | ||
+ | style="font-size:22px; line-height:130%; margin:7.1pt 47.75pt 0pt 49.15pt; orphans:0; text-indent:-19.2pt; widows:0"> | ||
+ | <span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">6.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">For further preservation, transfer | ||
+ | tubes to be stored at -80˚C after a period of</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-2.85pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">time.</span></p> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div id="myModal3" class="reveal-modal"> | ||
+ | <div style="overflow:scroll;height: 700px;width:1000px"> | ||
+ | <div> | ||
+ | <h1 style="margin:4.5pt 0pt 0pt 9pt; orphans:0; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:36px; font-weight:bold; ">LB Culture Medium with Phosphate Preparation</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-weight:bold; letter-spacing:-0.3pt; "> | ||
+ | </span> | ||
+ | </h1> | ||
+ | <p style="margin:7.1pt 0pt 0pt 28.2pt; orphans:0; text-indent:-19.2pt; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.05pt">A.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">      </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">Materials</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.2pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">(LB</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.2pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">culture</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.35pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">medium)</span> | ||
+ | </p> | ||
+ | <ol type="1" style="margin:0pt; padding-left:0pt"> | ||
+ | <li | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; margin:7.2pt 0pt 0pt 41.15pt; orphans:0; padding-left:5.25pt; text-indent:0pt; vertical-align:1pt; widows:0"> | ||
+ | <span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Refer</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">to</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">LB</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.3pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">Culture</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.35pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">Medium</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.15pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">Preparation</span> | ||
+ | </li> | ||
+ | </ol> | ||
+ | <p style="margin:7.1pt 0pt 0pt 28.2pt; orphans:0; text-indent:-19.2pt; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.05pt">B.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">      </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">Materials</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.05pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">(phosphate</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.1pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">solution)</span> | ||
+ | </p> | ||
+ | <p style="margin:0.25pt 0pt 0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:6pt; font-style:italic"> </span></p> | ||
+ | <table cellspacing="0" cellpadding="0" style="border-collapse:collapse; margin-left:6.5pt"> | ||
+ | <tr style="height:36pt"> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:8.95pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">Na</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:7.5pt">2</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">HPO</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:7.5pt">4</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">.12H</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:7.5pt">2</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">O</span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:9pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">25 mg</span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style="height:36pt"> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:8.9pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">NaH</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:7.5pt">2</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">PO</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:7.5pt">4</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">.2H</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:7.5pt">2</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">O</span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:8.95pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">13 mg</span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | <tr style="height:44.25pt"> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:0.4pt 0pt 0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:11pt; font-style:italic"> </span></p> | ||
+ | <p style="margin:0pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">ddH</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:7.5pt">2</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">O</span> | ||
+ | </p> | ||
+ | </td> | ||
+ | <td | ||
+ | style="border-bottom-color:#000000; border-bottom-style:solid; border-bottom-width:1pt; border-left-color:#000000; border-left-style:solid; border-left-width:1pt; border-right-color:#000000; border-right-style:solid; border-right-width:1pt; border-top-color:#000000; border-top-style:solid; border-top-width:1pt; vertical-align:top; width:224.75pt"> | ||
+ | <p style="margin:0.45pt 0pt 0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:11pt; font-style:italic"> </span></p> | ||
+ | <p style="margin:0pt 0pt 0pt 2.95pt; orphans:0; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">200 mL</span></p> | ||
+ | </td> | ||
+ | </tr> | ||
+ | </table> | ||
+ | <p style="margin:3pt 0pt 0pt 28.2pt; orphans:0; text-indent:-19.2pt; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.05pt">C.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">      </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">Protocol</span> | ||
+ | </p> | ||
+ | <ol type="1" style="margin:0pt; padding-left:0pt"> | ||
+ | <li | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; margin:7.05pt 0pt 0pt 41.15pt; orphans:0; padding-left:5.25pt; text-indent:0pt; vertical-align:1pt; widows:0"> | ||
+ | <span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">Weigh</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt; vertical-align:1pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">25</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt; vertical-align:1pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">mg</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt; vertical-align:1pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">Na</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:7.5pt">2</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">HPO</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:7.5pt">4</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:7.5pt; letter-spacing:0.95pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">and</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt; vertical-align:1pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">dissove</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.25pt; vertical-align:1pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">in</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt; vertical-align:1pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">100</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt; vertical-align:1pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">mL</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.6pt; vertical-align:1pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">ddH</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:7.5pt">2</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">O.</span> | ||
+ | </li> | ||
+ | <li | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; margin:7.05pt 0pt 0pt 41.15pt; orphans:0; padding-left:5.25pt; text-indent:0pt; vertical-align:1pt; widows:0"> | ||
+ | <span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">Weigh</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt; vertical-align:1pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">13</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt; vertical-align:1pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">mg</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt; vertical-align:1pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">NaH</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:7.5pt">2</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">PO</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:7.5pt">4</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:7.5pt; letter-spacing:0.95pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">and</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt; vertical-align:1pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">dissolve</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.25pt; vertical-align:1pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">in</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt; vertical-align:1pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">100</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt; vertical-align:1pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">mL</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.6pt; vertical-align:1pt"> | ||
+ | </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">ddH</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:7.5pt">2</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; vertical-align:1pt">O.</span> | ||
+ | </li> | ||
+ | <li | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; margin:7.2pt 0pt 0pt 41.15pt; orphans:0; padding-left:5.25pt; text-indent:0pt; vertical-align:1pt; widows:0"> | ||
+ | <span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Sterilize</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">solutions</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">at</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">121˚C</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">for</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">30</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">minutes</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">in</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">a</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">sterilization | ||
+ | pot.</span></li> | ||
+ | </ol> | ||
+ | <p style="margin:0pt; orphans:0; widows:0"><span | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:13pt"> </span></p> | ||
+ | <h1 style="margin:10.85pt 0pt 0pt 9pt; orphans:0; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:36px; font-weight:bold; letter-spacing:-0.1pt; ">Function Test Attempt 1</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-weight:bold; letter-spacing:-0.4pt; "> | ||
+ | </span> | ||
+ | </h1> | ||
+ | <p style="margin:7.1pt 0pt 0pt 28.2pt; orphans:0; text-indent:-19.2pt; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic; letter-spacing:-0.05pt">A.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">      </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">Protocol</span> | ||
+ | </p> | ||
+ | <p style="margin:7.05pt 0pt 0pt 46.4pt; orphans:0; text-indent:-19.25pt; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">1.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Sterilize</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">work</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">station</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">and work</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">near</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">a</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">lit</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">alcohol</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">burner.</span></p> | ||
+ | <p style="margin:7.05pt 0pt 0pt 46.4pt; orphans:0; text-indent:-19.25pt; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">2.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Prepare</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">marked 15</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">mL</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.55pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">test</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">tubes</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">for</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">testing.</span></p> | ||
+ | <p | ||
+ | style="font-size:22px; line-height:130%; margin:7.2pt 52.6pt 0pt 46.4pt; orphans:0; text-indent:-19.2pt; widows:0"> | ||
+ | <span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">3.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Distribute</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">4</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">mL</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.55pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">LB</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.1pt"> </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">culture</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.15pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">medium</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">without</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">KANA</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.7pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">antibiotics</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.1pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">to</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">each teast</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-2.85pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">tube.</span></p> | ||
+ | <p style="margin:2.9pt 0pt 0pt 46.4pt; orphans:0; text-indent:-19.25pt; widows:0"><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:normal">4.</span><span | ||
+ | style="font:7.0pt 'Times New Roman'">       </span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">Add</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">40</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">µL</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.55pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; font-style:italic">E.coli | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">to</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">its</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.2pt"> | ||
+ | </span><span style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">corresponding</span><span | ||
+ | style="color:#333333; font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:0.05pt"> | ||
+ | </span> | ||
+ | </p> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <!--bottom--> | ||
+ | <div style="background-color: #F9F9F9;"> | ||
+ | <div style="width: 80%;height: 1080px;margin: 0 auto;padding: 10% 0;"> | ||
+ | <span | ||
+ | style="font-size: 48px;display: block;padding-bottom: 70px;">CONTACT INFO</span> | ||
+ | <span | ||
+ | style="text-align: center;line-height: 90px;letter-spacing: 2px;font-size: 36px;display: block;font-family: -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen, Ubuntu, Cantarell, 'Open Sans', 'Helvetica Neue', sans-serif;"> | ||
+ | Please scan the following QR codes to find more about us on | ||
+ | </span> | ||
+ | <span | ||
+ | style="text-align: center;font-weight: bold;line-height: 40px;letter-spacing: 2px;font-size: 36px;display: block;font-family: -apple-system, BlinkMacSystemFont, 'Segoe UI', Roboto, Oxygen, Ubuntu, Cantarell, 'Open Sans', 'Helvetica Neue', sans-serif;"> | ||
+ | Weibo、Bilibili、Wechat ! | ||
+ | </span> | ||
+ | <img style="width:810px;height: 345px;margin: 100px auto;display: block;" src="https://static.igem.org/mediawiki/2021/1/1d/T--Shanghai_United--homepage06.jpg"> | ||
+ | </div> | ||
+ | </div> | ||
+ | <script> | ||
+ | (function($) { | ||
+ | |||
+ | /*--------------------------- | ||
+ | Defaults for Reveal | ||
+ | ----------------------------*/ | ||
+ | |||
+ | /*--------------------------- | ||
+ | Listener for data-reveal-id attributes | ||
+ | ----------------------------*/ | ||
+ | |||
+ | $('a[data-reveal-id]').live('click', function(e) { | ||
+ | e.preventDefault(); | ||
+ | var modalLocation = $(this).attr('data-reveal-id'); | ||
+ | $('#'+modalLocation).reveal($(this).data()); | ||
+ | }); | ||
+ | |||
+ | /*--------------------------- | ||
+ | Extend and Execute | ||
+ | ----------------------------*/ | ||
+ | |||
+ | $.fn.reveal = function(options) { | ||
+ | |||
+ | |||
+ | var defaults = { | ||
+ | animation: 'fadeAndPop', //fade, fadeAndPop, none | ||
+ | animationspeed: 300, //how fast animtions are | ||
+ | closeonbackgroundclick: true, //if you click background will modal close? | ||
+ | dismissmodalclass: 'close-reveal-modal' //the class of a button or element that will close an open modal | ||
+ | }; | ||
+ | |||
+ | //Extend dem' options | ||
+ | var options = $.extend({}, defaults, options); | ||
+ | |||
+ | return this.each(function() { | ||
+ | |||
+ | /*--------------------------- | ||
+ | Global Variables | ||
+ | ----------------------------*/ | ||
+ | var modal = $(this), | ||
+ | topMeasure = parseInt(modal.css('top')), | ||
+ | topOffset = modal.height() + topMeasure, | ||
+ | locked = false, | ||
+ | modalBG = $('.reveal-modal-bg'); | ||
+ | |||
+ | /*--------------------------- | ||
+ | Create Modal BG | ||
+ | ----------------------------*/ | ||
+ | if(modalBG.length == 0) { | ||
+ | modalBG = $('<div class="reveal-modal-bg" />').insertAfter(modal); | ||
+ | } | ||
+ | |||
+ | /*--------------------------- | ||
+ | Open & Close Animations | ||
+ | ----------------------------*/ | ||
+ | //Entrance Animations | ||
+ | modal.bind('reveal:open', function () { | ||
+ | modalBG.unbind('click.modalEvent'); | ||
+ | $('.' + options.dismissmodalclass).unbind('click.modalEvent'); | ||
+ | if(!locked) { | ||
+ | lockModal(); | ||
+ | if(options.animation == "fadeAndPop") { | ||
+ | modal.css({'top': $(document).scrollTop()-topOffset, 'opacity' : 0, 'visibility' : 'visible'}); | ||
+ | modalBG.fadeIn(options.animationspeed/2); | ||
+ | modal.delay(options.animationspeed/2).animate({ | ||
+ | "top": $(document).scrollTop()+topMeasure + 'px', | ||
+ | "opacity" : 1 | ||
+ | }, options.animationspeed,unlockModal()); | ||
+ | } | ||
+ | if(options.animation == "fade") { | ||
+ | modal.css({'opacity' : 0, 'visibility' : 'visible', 'top': $(document).scrollTop()+topMeasure}); | ||
+ | modalBG.fadeIn(options.animationspeed/2); | ||
+ | modal.delay(options.animationspeed/2).animate({ | ||
+ | "opacity" : 1 | ||
+ | }, options.animationspeed,unlockModal()); | ||
+ | } | ||
+ | if(options.animation == "none") { | ||
+ | modal.css({'visibility' : 'visible', 'top':$(document).scrollTop()+topMeasure}); | ||
+ | modalBG.css({"display":"block"}); | ||
+ | unlockModal() | ||
+ | } | ||
+ | } | ||
+ | modal.unbind('reveal:open'); | ||
+ | }); | ||
+ | |||
+ | //Closing Animation | ||
+ | modal.bind('reveal:close', function () { | ||
+ | if(!locked) { | ||
+ | lockModal(); | ||
+ | if(options.animation == "fadeAndPop") { | ||
+ | modalBG.delay(options.animationspeed).fadeOut(options.animationspeed); | ||
+ | modal.animate({ | ||
+ | "top": $(document).scrollTop()-topOffset + 'px', | ||
+ | "opacity" : 0 | ||
+ | }, options.animationspeed/2, function() { | ||
+ | modal.css({'top':topMeasure, 'opacity' : 1, 'visibility' : 'hidden'}); | ||
+ | unlockModal(); | ||
+ | }); | ||
+ | } | ||
+ | if(options.animation == "fade") { | ||
+ | modalBG.delay(options.animationspeed).fadeOut(options.animationspeed); | ||
+ | modal.animate({ | ||
+ | "opacity" : 0 | ||
+ | }, options.animationspeed, function() { | ||
+ | modal.css({'opacity' : 1, 'visibility' : 'hidden', 'top' : topMeasure}); | ||
+ | unlockModal(); | ||
+ | }); | ||
+ | } | ||
+ | if(options.animation == "none") { | ||
+ | modal.css({'visibility' : 'hidden', 'top' : topMeasure}); | ||
+ | modalBG.css({'display' : 'none'}); | ||
+ | } | ||
+ | } | ||
+ | modal.unbind('reveal:close'); | ||
+ | }); | ||
+ | |||
+ | /*--------------------------- | ||
+ | Open and add Closing Listeners | ||
+ | ----------------------------*/ | ||
+ | //Open Modal Immediately | ||
+ | modal.trigger('reveal:open') | ||
+ | |||
+ | //Close Modal Listeners | ||
+ | var closeButton = $('.' + options.dismissmodalclass).bind('click.modalEvent', function () { | ||
+ | modal.trigger('reveal:close') | ||
+ | }); | ||
+ | |||
+ | if(options.closeonbackgroundclick) { | ||
+ | modalBG.css({"cursor":"pointer"}) | ||
+ | modalBG.bind('click.modalEvent', function () { | ||
+ | modal.trigger('reveal:close') | ||
+ | }); | ||
+ | } | ||
+ | $('body').keyup(function(e) { | ||
+ | if(e.which===27){ modal.trigger('reveal:close'); } // 27 is the keycode for the Escape key | ||
+ | }); | ||
+ | |||
+ | |||
+ | /*--------------------------- | ||
+ | Animations Locks | ||
+ | ----------------------------*/ | ||
+ | function unlockModal() { | ||
+ | locked = false; | ||
+ | } | ||
+ | function lockModal() { | ||
+ | locked = true; | ||
+ | } | ||
+ | |||
+ | });//each call | ||
+ | }//orbit plugin call | ||
+ | })(jQuery); | ||
+ | </script> | ||
+ | </body> | ||
+ | <script src="https://2021.igem.org/Team:Shanghai_United/Template:JS?action=raw&ctype=text/javascript"></script> | ||
+ | <script> | ||
+ | //由于模块都一次性加载,因此不用执行 layui.use() 来加载对应模块,直接使用即可: | ||
+ | ; | ||
+ | ! function () { | ||
+ | var layer = layui.layer, | ||
+ | form = layui.form; | ||
+ | }(); | ||
+ | |||
+ | layui.use('carousel', function () { | ||
+ | var carousel = layui.carousel; | ||
+ | //建造实例 | ||
+ | carousel.render({ | ||
+ | elem: '#test1' | ||
+ | , width: '100%' //设置容器宽度 | ||
+ | , arrow: 'always' //始终显示箭头 | ||
+ | //,anim: 'updown' //切换动画方式 | ||
+ | }); | ||
+ | }); | ||
+ | |||
+ | |||
+ | </script> | ||
+ | |||
+ | </html> | ||
+ | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | ||
style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">bottom of</span><span | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px">bottom of</span><span | ||
style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span | style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span><span |
Revision as of 09:46, 6 October 2021
![](https://static.igem.org/mediawiki/2021/2/27/T--Shanghai_United--homepage01.png)
![](https://static.igem.org/mediawiki/2021/a/a5/T--Shanghai_United--Description00.png)
PCR of the target Gene
A. Materials
ddH2O |
32 μL |
dNTP |
5 μL |
MgSO4 |
4 μL |
Forward primer |
1 μL |
Reverse primer |
1 μL |
DNA template |
1 μL |
KOD+ buffer |
5 μL |
KOD+ |
1 μL |
B. Protocol
1. Prepare and mark PCR tubes and align tubes in order on tube rack.
2. Under normal circumstances, ddH2O should be added into tubes first, and enzymes should be added last. All other materials uncluding, but are not limited to dNTP, primers, buffers, and templates should be added in between.
3. PCR tubes should then be centrifuged briefly to ensure all liquids collect at the bottom of the tube.
4. Move PCR tubes to PCR machine to initiate the process.
C. Reacting conditions
98 °C |
10 minutes |
1 cycle |
98 °C |
15 seconds |
26 cycles |
55 °C |
15 seconds |
|
68 °C |
1 minute |
68 °C |
10 minutes |
1 cycle |
4 °C |
Infinite |
N/A |
Gel Electrophoresis of the Gene PCR Product A. Materials
Loading buffer & DNA dye |
1 μL |
PCR product |
5 μL |
TAE buffer |
200 mL |
Agarose |
2 g |
B. Protocol
1. Mix TAE buffer and agarose powder in a ratio of 100 mL TAE buffer : 1 g agarose powder.
2. Heat the mixed solution in a microwave until agarose powder completely dissolves into TAE buffer.
3. Pour the liquid into gel trays of suitable sizes. Insert a comb onto the tray to
4. Mix 6x loading buffer premixed with DNA dye with PCR product in a ratio of 1 unit loading buffer : 5 units PCR product.
5. Load proper ladder into first well of gel.
6. Load samples into wells.
7. Perform electrophoresis at 185 V for 10 minutes.
8. Harvest gel, take image of gel under untraviolet light, and analyze results.
PCR Product Clean Up
A. Protocol (MN NucleoSpin Gel and PCR Clean-up Kit)
1. Combine obtained PCR products into one centrifuge tube.
2. Add buffer NTI to the product in a ratio of 2 μL buffer NTI : 1 μL PCR product to allow DNA to bind to the membrane.
3. Transfer the solution to a column placed within a collection tube. Centrifuge
at 12000 rpm for 30 seconds.
4. Transfer the liquid in the collection tube to the column. Centrifuge at 12000 rpm for 30 seconds.
5. Dispose of the liquid in the collection tube. Add 700 μL buffer NT3 to wash the membrane. Centrifuge at 12000 rpm for 30 seconds.
6. Repeat step 5.
7. Add 30 μL ddH2O preheated to 65 ˚C to the column. Centrifuge at 12000 rpm for 30 seconds.
B. Buffers
Buffer NTI: Adjust DNA binding conditions and allow DNA to bind to membrane.
Buffer NT3: Wash membrane.
Plasmid Extraction
A. Protocol (TIAN Prep Mini Plasmid Kit)
1. Harvest and transfer cultivated bacteria into marked 2 mL centrifuge tubes.
2. Centrifuge tubes at 12000 rpm for 30 seconds to deposit bacteria to the bottom of the tube. Dispose of the clear liquid on top.
3. Add 250 μL buffer P1 into the 2 mL centrifuge tubes to levitete the bacteria persent in the tubes.
4. Add 250 μL buffer P2 to the tubes to lyse bacterial cell wall. Mix the solution gently; do not vortex.
5. Add 350 μL buffer P3 to the tubes to neutralize buffer P2 and cause proteins in the solution to percipitate. Mix the solution gently; do not vortex.
6. Centrifuge the solution at 12000 rpm for 10 minutes to collect percitipation to the bottom of the tube.
7. Add 500 μL buffer BL to a column placed within a collection tube to wash the membrane. Centrifuge at 12000 rpm for 30 seconds.
8. Transfer the clear liquid obtained in step 6 to the column washed in step 7 and centrifuge the column at 12000 rpm for 30 seconds.
9. Dispose the clear liquid in the collection tube. Add 500 μL buffer PD to the column and centrifuge at 12000 rpm for 30 seconds.
10. Dispose of the liquid in the collection tube. Add 600 μL buffer PW to the column. Centrifuge at 12000 rpm for 30 seconds.
11. Repeat step 10.
12. Dispose of the clear liquid in the collection tube. Centrifuge at 12000 rpm for 1 minute.
13. Transfer the column to a new 2 mL centrifiuge tube. Dispose of the collection tube.
14. Add 40 μL ddH2O preheated to 65 °C to the column. Centrifuge at 12000 rpm for 30 seconds.
B. Buffers
Buffer P1: Levitate bacteria and lyse RNA. Buffer P2: Lyse bacteria cell wall.
Buffer P3: Neutralize P2 and cause proteins to precipitate. Buffer BL: Wash memnbrane pre-use.
Buffer PD: Wash proteins off the membrane. Buffer PW: Wash salts off the membrane.
Restriction Enzyme Digestion
A. Materials
ARSA/ARSR/ARSD |
|
10x NEB buffer |
5 μL |
ARSA/D/R Plasmid (300 ng/μL) |
10 μL |
BsaI restriction enzyme |
1 μL |
ddH2O |
34 μL |
amilGFP |
|
10x NEB buffer |
5 μL |
amilGFP (400 ng/μL) |
10 μL |
BsaI |
1 μL |
DpnI |
1 μL |
ddH2O |
33 μL |
B. Protocol
1. Mix the materials in a 1.5 mL centrifuge tube.
2. Place the centrifuge tube in 37 °C metal bath for at least 15 minutes.
Gel Electrophoresis of Restriction Enzyme Digestion Product
A. Materials
Loading buffer with DNA dye |
10 μL |
Digestion product |
50 μL |
B. Protocol
1. Mix 6x loading buffer premixed with DNA dye with PCR product in a ratio of 1 unit loading buffer : 5 units PCR product.
2. Load proper ladder into first well of gel.
3. Load samples into wells.
4. Perform electrophoresis at 185 V for 10 minutes.
5. Harvest gel, take image of gel under untraviolet light, and analyze results.
Agarose Gel DNA Extraction
A. Protocol (MN NucleoSpin Gel and PCR Clean-up Kit)
1. Collect gel with desired DNA fragments into 2 mL centrifuge tubes. Weigh the mass of gels on an electronic balance.
2. Add buffer NTI to the tube in a ratio of 2 μL buffer NTI : 1 mg agarose gel.
3. Heat the mixture at 50 ˚C in a metal bath until the gel dissolves completely.
4. Transfer the solution to a new column placed within a collection tube. Centrifuge at 12000 rpm for 30 seconds.
5. Transfer the liquid in the collection tube to the column. Centrifuge at 12000 rpm for 30 seconds.
6. Dispose of the clear liquid in the collection tube. Add 700 μL buffer NT3 to
the column. Centrifuge at 12000 rpm for 30 seconds.
7. Repeat step 6.
8. Dispose of the liquid in the collection tube. Centrifuge at 12000 rpm for 1 minute.
9. Add 30 μL ddH2O preheated to 65 ˚C to the column. Centrifuge at 12000 rpm for 30 seconds.
T4 DNA Ligase Ligation
A. Materials
ARSA/R/D plasmid (30 ng/μL) |
1.5 μL |
GFP (100 ng/μL) |
4.5 μL |
T4 DNA ligase |
1 μL |
T4 DNA ligase buffer |
1 μL |
ddH2O |
2 μL |
B. Protocol
1. Add ddH2O, T4 DNA ligase buffer, and T4 DNA ligase into a PCR tube with GFP gene and ARS plasmid in a ratio of 1 units ARS plasmid : 10 unit GFP gene.
2. Transfer the solution to PCR machine at 16˚C.
Transformation of DH5-α E. coli Using Heat Shock
A. Protocol
1. Mix the tube introduced DNA material with DH5-α competent cells. The volumn of DNA material must not exceed 10% of that of the competent cells.
2. Gently fick the tube 4-5 times to mix competent cells and DNA material. Do not vortex.
3. Place the tube on ice for 30 minutes.
4. Place the tube at exactly 42 ˚C for 90 seconds to perform heat shock.
5. Immediately place the tube back on ice after heat shock. Keep the tube on ice for 2-3 minutes.
6. Add 900 μL LB culture medium without KANA antibiotics to the tube. Incubate the transformed bacteria at 37 ˚C for 30 minutes.
7. Centrifuge the tube at 12000 rpm for 30 seconds.
8. Dispose half of the clear liquid in the centrifuge tube. Mix and levitate the bacteria on the bottom of the tube with a micropipette.
9. In a sterile environment, transfer the transformed bacteria to prepared agar plates.
Colony PCR Identification
A. Materials
2x PCR mix |
10 μL |
Forward primer |
0.5 μL |
Reverse primer |
0.5 μL |
Template |
Desired E. Coli |
ddH2O |
9 μL |
B. Protocol
1. Refer to PCR of amilGFP Gene, Jul. 28, 2021.
2. Notes to this reaction:
a. Primers were delivered in the form of dry powder. Centrifuge primers at 12000 rpm for 5 minutes to deposit powder at the bottom of its tube.
b. Dilute primers to a concentration of 10 nmol/ml.
c. Mix the 2x PCR mix, R.primer, Templalate, ddH2O as master mix.
C. Reacting conditions
98 °C |
10 minutes |
1 cycle |
98 °C |
15 seconds |
26 cycles |
55 °C |
15 seconds |
|
72 °C |
30 seconds |
72 °C |
5 minutes |
1 cycle |
4 °C |
Infinite |
N/A |
LB Culture Medium Preperation
- Formula
Yeast extract |
5 g/L |
Tryptone |
10 g/L |
NaCl |
10 g/L |
Agar* |
1.5% of total mass |
ddH2O |
Desired volume |
*Agar is only necessary when LB culture medium is prepared as solid agar gel.
- Sterilization
Sterilize at 121 °C for 30 minutes.
- KANA antibiotics
Concentration of KANA antibiotics in solution is 50 mg/μL. Functional concentration of KANA antibiotics is 50 μg/μL.
E. coli Cultivation
A. Protocol
1. Clean workstation with 75% ethanol and work near a lit alcohol lamp in steril conditions.
2. Prepare LB culture medium without KANA antibiotics.
3. Prepare three lines of E. coli with KANA resistance and ARSA, ARSD, or ARSR plasmids depending on the cell line.
4. Add KANA antibiotics to LB culture medium in a ratio of 1 μL KANA antibiotics : 1 mL LB culture medium.
5. Prepare three marked 15 mL centrifure tubes. Distribute 4 mL LB culture medium to each tube.
6. Transfer 10 μL of bacterial solution to its corresponding tube.
7. Cultivate in a shaker in 37 °C at 220 rpm.
Preparation of Agar Plates
A. Protocol
1. Heat the pre-prepared solid LB culture medium in a microwave until the solid completely melts.
2. Add KANA antibiotics in a ratio of 1 μL KANA antibiotics : 1000 μL culture medium. Mix the solutions throughly.
3. In a sterile environment, carefully pour approximately 20-30 mL culture medium to each empty plate.
4. Tilt the plate genly to cover the plate completely with culture medium. Set the plates still and wait for culture medium to cool and solidify.
Preservation of Transformed E. coli
A. Materials
Glycerol |
900 µL |
LB culture medium with E.coli |
900 µL |
B. Protocol
1. Sterilize work station and work near a lit alcohol burner. Prepare and mark 2 mL centrifuge tubes.
2. Add 900 µL glycerol to a 2 mL centrifuge tube.
3. Add 900 µL LB culture medium with E.coli to its corresponding tube.
4. Apply laboratory film to seal every centrifuge tube.
5. Store at -20˚C.
6. For further preservation, transfer tubes to be stored at -80˚C after a period of time.
LB Culture Medium with Phosphate Preparation
A. Materials (LB culture medium)
- Refer to LB Culture Medium Preparation
B. Materials (phosphate solution)
Na2HPO4.12H2O |
25 mg |
NaH2PO4.2H2O |
13 mg |
ddH2O |
200 mL |
C. Protocol
- Weigh 25 mg Na2HPO4 and dissove in 100 mL ddH2O.
- Weigh 13 mg NaH2PO4 and dissolve in 100 mL ddH2O.
- Sterilize solutions at 121˚C for 30 minutes in a sterilization pot.
Function Test Attempt 1
A. Protocol
1. Sterilize work station and work near a lit alcohol burner.
2. Prepare marked 15 mL test tubes for testing.
3. Distribute 4 mL LB culture medium without KANA antibiotics to each teast tube.
4. Add 40 µL E.coli to its corresponding
![](https://static.igem.org/mediawiki/2021/1/1d/T--Shanghai_United--homepage06.jpg)
style="font-family:'Segoe UI', Tahoma, Geneva, Verdana, sans-serif; font-size:22px; letter-spacing:-0.05pt"> </span>bottom of the tube with a micropipette.</p>
9. In a sterile environment, transfer the transformed bacteria to prepared agar plates.
Contents
Colony PCR Identification
A. Materials
2x PCR mix |
10 μL |
Forward primer |
0.5 μL |
Reverse primer |
0.5 μL |
Template |
Desired E. Coli |
ddH2O |
9 μL |
B. Protocol
1. Refer to PCR of amilGFP Gene, Jul. 28, 2021.
2. Notes to this reaction:
a. Primers were delivered in the form of dry powder. Centrifuge primers at 12000 rpm for 5 minutes to deposit powder at the bottom of its tube.
b. Dilute primers to a concentration of 10 nmol/ml.
c. Mix the 2x PCR mix, R.primer, Templalate, ddH2O as master mix.
C. Reacting conditions
98 °C |
10 minutes |
1 cycle |
98 °C |
15 seconds |
26 cycles |
55 °C |
15 seconds |
|
72 °C |
30 seconds |
</div>
72 °C |
5 minutes |
1 cycle |
4 °C |
Infinite |
N/A |
</div> </div>
LB Culture Medium Preperation
- Formula
Yeast extract |
5 g/L |
Tryptone |
10 g/L |
NaCl |
10 g/L |
Agar* |
1.5% of total mass |
ddH2O |
Desired volume |
*Agar is only necessary when LB culture medium is prepared as solid agar gel.
- Sterilization
Sterilize at 121 °C for 30 minutes.
- KANA antibiotics
Concentration of KANA antibiotics in solution is 50 mg/μL. Functional concentration of KANA antibiotics is 50 μg/μL.
E. coli Cultivation <p style="margin:7.05pt 0pt 0pt 28.2pt; orphans:0; text-indent:-19.2pt; widows:0">A. Protocol
1. Clean workstation with 75% ethanol and work near a lit alcohol lamp in steril conditions.
2. Prepare LB culture medium without KANA antibiotics.
3. Prepare three lines of E. coli with KANA resistance and ARSA, ARSD, or ARSR plasmids depending on the cell line.
4. Add KANA antibiotics to LB culture medium in a ratio of 1 μL KANA antibiotics : 1 mL LB culture medium.
5. Prepare three marked 15 mL centrifure tubes. Distribute 4 mL LB culture medium to each tube.
6. Transfer 10 μL of bacterial solution to its corresponding tube.
7. Cultivate in a shaker in 37 °C at 220 rpm.
Preparation of Agar Plates
A. Protocol
1. Heat the pre-prepared solid LB culture medium in a microwave until the solid completely melts.
2. Add KANA antibiotics in a ratio of 1 μL KANA antibiotics : 1000 μL culture medium. Mix the solutions throughly.
3. In a sterile environment, carefully pour approximately 20-30 mL culture medium to each empty plate.
4. Tilt the plate genly to cover the plate completely with culture medium. Set the plates still and wait for culture medium to cool and solidify.
Preservation of Transformed E. coli
A. Materials
Glycerol |
900 µL |
LB culture medium with E.coli |
900 µL |
B. Protocol
1. Sterilize work station and work near a lit alcohol burner. Prepare and mark 2 mL centrifuge tubes.
2. Add 900 µL glycerol to a 2 mL centrifuge tube.
3. Add 900 µL LB culture medium with E.coli to its corresponding tube.
4. Apply laboratory film to seal every centrifuge tube.
5. Store at -20˚C.
6. For further preservation, transfer tubes to be stored at -80˚C after a period of time.
LB Culture Medium with Phosphate Preparation
A. Materials (LB culture medium)
- Refer to LB Culture Medium Preparation
B. Materials (phosphate solution)
Na2HPO4.12H2O |
25 mg |
NaH2PO4.2H2O |
13 mg |
ddH2O |
200 mL |
C. Protocol
- Weigh 25 mg Na2HPO4 and dissove in 100 mL ddH2O.
- Weigh 13 mg NaH2PO4 and dissolve in 100 mL ddH2O.
- Sterilize solutions at 121˚C for 30 minutes in a sterilization pot.
Function Test Attempt 1
A. Protocol
1. Sterilize work station and work near a lit alcohol burner.
2. Prepare marked 15 mL test tubes for testing.
3. Distribute 4 mL LB culture medium without KANA antibiotics to each teast tube.
4. Add 40 µL E.coli to its corresponding
</div>
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