Difference between revisions of "Team:Shanghai City United/Description"

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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the <a href="https://2021.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2021.igem.org/Judging/Awards"> award listed below</a>. </p>
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<h1>Project Description </h1>
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<h3>Bronze Medal Criterion #3</h3>
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<p>Describe how and why you chose your iGEM project.
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<br><br>
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Please see the <a href="https://2021.igem.org/Judging/Medals">2021 Medals Page</a> for more information.
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<h3>What should this page contain?</h3>
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<li> A clear and concise description of your project.</li>
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<li>A detailed explanation of why your team chose to work on this particular project.</li>
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<li>References and sources to document your research.</li>
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<li>Use illustrations and other visual resources to explain your project.</li>
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<h3>Inspiration</h3>
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<p>See how other teams have described and presented their projects: </p>
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<ul>
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<li><a href="https://2019.igem.org/Team:Leiden/Description">2019 Leiden</a></li>
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<li><a href="https://2019.igem.org/Team:ITESO_Guadalajara/Description">2019 ITESO Guadalajara</a></li>
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<li><a href="https://2020.igem.org/Team:Technion-Israel/Description">2020 Technion Israel</a></li>
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<li><a href="https://2020.igem.org/Team:Botchan_Lab_Tokyo/Description">2020 Botchan Lab Tokyo</a></li>
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<li><a href="https://2020.igem.org/Team:St_Andrews/Description">2020 St Andrews</a></li>
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<li><a href="https://2020.igem.org/Team:MIT/Description">2020 MIT</a></li>
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<h3>Advice on writing your Project Description</h3>
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We encourage you to put up a lot of information and content on your wiki, but we also encourage you to include summaries as much as possible. If you think of the sections in your project description as the sections in a publication, you should try to be concise, accurate, and unambiguous in your achievements. Your Project Description should include more information than your project abstract.
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<h3>References</h3>
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<p>iGEM teams are encouraged to record references you use during the course of your research. They should be posted somewhere on your wiki so that judges and other visitors can see how you thought about your project and what works inspired you.</p>
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        <div class="sub-title">PROJECT DESCRIPTION</div>
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        <div class="article-title">Inspiration</div>
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        <div class="img-wrap no-margin">
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            <img style="width: 100%;"
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                src="https://static.igem.org/mediawiki/2021/9/96/T--Shanghai_City_United--Description02.png" alt="" />
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        <div class="article-content margin-bottom-45">Feed grains are whole grains such as corn, wheat and barley used
 +
            in the feeding of
 +
            livestock and poultry<br />◇ Barley is a grain with high energy and protein used for feed of ruminate
 +
            livestock, such as cattle. It can be used in growing and finishing diets or used in blends or feed
 +
            mixtures.<br />◇ Feed wheat is wheat that didn't reach standards needed for milling due to disease, drought
 +
            or sprouting. This feed-grade wheat is a palatable, digestible energy source for cattle, pigs and
 +
            poultry.<br />◇ Yellow corn is a major source of starch and is the best suited for processing and feedstock
 +
            for cattle and poultry and is available in bulk vessel, truck, rail or barge.</div>
 +
        <div class="article-content" style="margin-bottom: 95px;">At present, corn is the main fodder in the feed
 +
            industry. Due to the increasing
 +
            shortage of raw materials and the rising price, the development of the feed industry has been greatly
 +
            limited. One of the important measures to alleviate the shortages of corn is to fully develop wheat, grain,
 +
            and bran, which are abundant in China, to replace corn. However, the cell walls of cereals such as wheat,
 +
            cereal, and bran contain anti-nutritional factors such as arabinoxylan and non-starch polysaccharide (NSP),
 +
            which will affect the digestibility of nutrients in single-stomach animals and the absorption of nutrients
 +
            in poultry. Arabinoxylan is a polysaccharide found in rice bran (hemicellulose B) edible fiber. Therefore,
 +
            we decide to aim at adding xylanase to the feed to degrade arabinoxylan, so as to improve the feed
 +
            absorption efficiency.</div>
 +
        <div class="article-title">Grain-Fed+ Project</div>
 +
        <div class="img-wrap no-margin">
 +
            <img style="width: 247px;height:452px;"
 +
                src="https://static.igem.org/mediawiki/2021/6/6e/T--Shanghai_City_United--Description03.png" alt="" />
 +
        </div>
 +
        <div class="article-content" style="margin-bottom: 95px;">Our project is to design an edible "drink" for
 +
            monogastric
 +
            animals, mainly poultry
 +
            through biosynthesis technology. <br />The core product will be a probiotic that can produce xylanase. In
 +
            this project, we designed to construct the target plasmids through PCR amplifing genes, electrophoresis
 +
            appraisal, and ligation experiements, then we transformed them into E. coli and Lactobacillus reuteri for
 +
            further performance anaylisis.<br />Compared to traditional feed, our drink contains the probiotics that
 +
            could help poultry digest xylan, thus increasing feed efficiency. In this way, not only can the time and
 +
            economic cost of feeding be saved, but also the gastrointestinal tract of the animal can be protected and
 +
            thus the disease rate can be reduced.</div>
 +
        <div class="article-title">Reference</div>
 +
        <div class="article-content">1. Beasley S S,Takala T M， Reunanen J， Apajalahti J， Saris P
 +
            E.2004.Characterization and electrotransformation of Lactobacillus crispatus isolated from chicken crop and
 +
            intestine. Poult Science，83(1)：45-48;</div>
 +
        <div class="article-content">2. De Vos W M.1999.Gene expression systems for lactic acid bacteria. Current
 +
            Opinion in Microbiology，2(3)：289-295;</div>
 +
        <div class="article-content">3. Silversides F G， Scott T A， Korver D R，Afsharmanesh M，Hruby M.2006. A study on
 +
            the interaction of xylanase and phytase enzymes in wheat-based diets fed to commercial white and brown egg
 +
            laying hens.Poultry Science，85(2)：297-305;</div>
 +
        <div class="article-content">4. 崔罗生，祝茂生，徐顺清，朱辉，梁运祥，张忠明.2009.黑曲霉木聚糖酶基因(xyn A)
 +
            在大肠杆菌中的表达及酶学分析.华中农业大学学报，28(1)：48-53；</div>
 +
        <div class="article-content">5. 李慧.2010. 蛋白酶和木聚糖酶对肉鸡生长性能，消化机能及血液指标的影响.[研究生学位论文].杨凌：西北农林科技大学；</div>
 +
        <div class="article-content">6. https://wenku.baidu.com/view/bb1a6d76590216fc700abb68a98271fe910eafb9.html</div>
 +
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Revision as of 08:39, 2 October 2021

Shanghai_City_United

PROJECT DESCRIPTION
Inspiration
Feed grains are whole grains such as corn, wheat and barley used in the feeding of livestock and poultry
◇ Barley is a grain with high energy and protein used for feed of ruminate livestock, such as cattle. It can be used in growing and finishing diets or used in blends or feed mixtures.
◇ Feed wheat is wheat that didn't reach standards needed for milling due to disease, drought or sprouting. This feed-grade wheat is a palatable, digestible energy source for cattle, pigs and poultry.
◇ Yellow corn is a major source of starch and is the best suited for processing and feedstock for cattle and poultry and is available in bulk vessel, truck, rail or barge.
At present, corn is the main fodder in the feed industry. Due to the increasing shortage of raw materials and the rising price, the development of the feed industry has been greatly limited. One of the important measures to alleviate the shortages of corn is to fully develop wheat, grain, and bran, which are abundant in China, to replace corn. However, the cell walls of cereals such as wheat, cereal, and bran contain anti-nutritional factors such as arabinoxylan and non-starch polysaccharide (NSP), which will affect the digestibility of nutrients in single-stomach animals and the absorption of nutrients in poultry. Arabinoxylan is a polysaccharide found in rice bran (hemicellulose B) edible fiber. Therefore, we decide to aim at adding xylanase to the feed to degrade arabinoxylan, so as to improve the feed absorption efficiency.
Grain-Fed+ Project
Our project is to design an edible "drink" for monogastric animals, mainly poultry through biosynthesis technology.
The core product will be a probiotic that can produce xylanase. In this project, we designed to construct the target plasmids through PCR amplifing genes, electrophoresis appraisal, and ligation experiements, then we transformed them into E. coli and Lactobacillus reuteri for further performance anaylisis.
Compared to traditional feed, our drink contains the probiotics that could help poultry digest xylan, thus increasing feed efficiency. In this way, not only can the time and economic cost of feeding be saved, but also the gastrointestinal tract of the animal can be protected and thus the disease rate can be reduced.
Reference
1. Beasley S S,Takala T M， Reunanen J， Apajalahti J， Saris P E.2004.Characterization and electrotransformation of Lactobacillus crispatus isolated from chicken crop and intestine. Poult Science，83(1)：45-48;
2. De Vos W M.1999.Gene expression systems for lactic acid bacteria. Current Opinion in Microbiology，2(3)：289-295;
3. Silversides F G， Scott T A， Korver D R，Afsharmanesh M，Hruby M.2006. A study on the interaction of xylanase and phytase enzymes in wheat-based diets fed to commercial white and brown egg laying hens.Poultry Science，85(2)：297-305;
4. 崔罗生，祝茂生，徐顺清，朱辉，梁运祥，张忠明.2009.黑曲霉木聚糖酶基因(xyn A) 在大肠杆菌中的表达及酶学分析.华中农业大学学报，28(1)：48-53；
5. 李慧.2010. 蛋白酶和木聚糖酶对肉鸡生长性能，消化机能及血液指标的影响.[研究生学位论文].杨凌：西北农林科技大学；
6. https://wenku.baidu.com/view/bb1a6d76590216fc700abb68a98271fe910eafb9.html
CONTACT INFO

Email: igemteamceres@gmail.com

WeChat Official Account: iGEM Ceres