Team:XMU-China/Parts

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Parts

This year, among the 99 parts designed, 39 of them are basic parts and the rest are composite parts. The parts are all designed by ourselves and most of them have been tested to insure their reliability. You can click here to learn more about our project to see their specific function, or click on the part numbers when browse website ( parts.igem.org ).

Basic Parts

Among the 38 basic parts designed this year by ourselves, BBa_K3739000 is our favorite one. In our project, the gene comes from Pseudomonas putida and is expressed in chassis bacteria to produce histidine ammonia-lyase. Besides, many other basic parts have also been designed to construct our project.


Favorite Name Type Description
  		• BBa_K3739000 Coding his-hutH
    BBa_K3739001 Coding his-CBM-GFP
    BBa_K3739002 Coding his-GFP-CBM
    BBa_K3739003 Coding his-GFP
    BBa_K3739004 Coding Aly01-his-3Spycatcher
    BBa_K3739005 Coding Aly01-his-3Spytag-elastin linker-lecA
    BBa_K3739006 Coding Aly01-his-hutH
    BBa_K3739007 Coding Aly01-his-GFP
    BBa_K3739008 Coding Aly01-his-CBM-hutH
    BBa_K3739009 Coding LMT-his-hutH
    BBa_K3739010 Coding LMT-his-GFP
    BBa_K3739011 Coding LMT-his-CBM-hutH
    BBa_K3739012 Coding his-rhlA
    BBa_K3739013 Coding his-rhlB
    BBa_K3739014 Coding tnaA-his
    BBa_K3739015 Coding PPO-his
    BBa_K3739016 Coding AKTA-his-Mfp5
    BBa_K3739017 Coding LMT-PPO-his
    BBa_K3739018 Coding Aly01-PPO-his
    BBa_K3739019 Coding LamB-GFP
    BBa_K3739020 Coding LC1KR2-GFP
    BBa_K3739021 Coding OmpA-GFP
    BBa_K3739022 Coding pelB-Ag43-160N-his-GFP
    BBa_K3739023 Coding pelB-Ag43-160N-Tevp
    BBa_K3739024 Coding LamB-LC1KR2-GFP
    BBa_K3739025 Coding OmpA-LC1KR2-GFP
    BBa_K3739026 Coding LamB1-linker-LamB2-his
    BBa_K3739027 Coding LamB1-LC1KR2-LamB2-his
    BBa_K3739028 Coding blrA
    BBa_K3739029 Coding rrnB T1
    BBa_K3739030 Coding blrA-GFP
    BBa_K3739060 Coding pDawn (LacIq-yf1-fixJ-pFixK2-cI-pR)
    BBa_K3739080 Coding Aly01-his-3*Spytag-elastin linker-hutH
    BBa_K3739081 Coding his-CBM-hutH
    BBa_K3739082 Coding Aly01-his-hutH-FT
    BBa_K3739083 Coding LMT-his-hutH-FT
    BBa_K3739086 Coding his-hutH-FT
    BBa_K3739100 Coding INPNC-his-SpyCatcher
    BBa_K3739101 Coding his-SpyTag-GFP
    Composite Parts

    We have designed a series of composite parts this year, and most of them could be useful tools for our design. And our favorite composite part is BBa_K3739104, which contains the sequence of the signal peptide-Aly01 to secrect our favorite basic part-Huth outside the cell.

    Aly01 is alginate lyase from Vibrio natriegens SK42.001, which is secreted out and able to digest alginate to unsaturated alginate oligosaccharide. Its signal peptide (named Aly01 in our parts), which is fused with heterogenous protein, is performed well in E. coli. It is implied that the heterogenous protein fused with Aly01 signal peptide may be also secreted efficiently.


    Favorite Name Type Description
    BBa_K3739031 Composite J23100-B0030-his-hutH-B0010
    BBa_K3739032 Composite J23100-B0030-his-CBM-GFP-B0010
    BBa_K3739033 Composite J23100-B0030-his-GFP-CBM-B0010
    BBa_K3739034 Composite J23100-B0030-Aly01-his-3Spycatcher-B0010
    BBa_K3739035 Composite J23100-B0030-Aly01-his-3Spytag-elastin linker-lecA-B0010
    BBa_K3739036 Composite J23100-B0030-Aly01-his-CBM-hutH-B0010
    BBa_K3739037 Composite J23100-B0030-Aly01-his-hutH-B0010
    BBa_K3739038 Composite J23100-B0030-Aly01-his-GFP-B0010
    BBa_K3739039 Composite J23100-B0030-LMT-his-CBM-hutH-B0010
    BBa_K3739040 Composite J23100-B0030-LMT-his-hutH-B0010
    BBa_K3739041 Composite J23100-B0030-LMT-his-GFP-B0010
    BBa_K3739042 Composite J23100-B0030-AKTA-his-Mfp5-B0010
    BBa_K3739043 Composite J23100-B0030-his-GFP-B0010
    BBa_K3739044 Composite J23100-B0030-tnaA-his-B0010
    BBa_K3739045 Composite J23100-B0030-PPO-his-B0010
    BBa_K3739046 Composite J23100-B0034-his-rhlA-B0015
    BBa_K3739047 Composite J23100-B0034-his-rhlB-B0015
    BBa_K3739048 Composite J23100-B0030-LamB-GFP-B0010
    BBa_K3739049 Composite J23100-B0030-OmpA-GFP-B0010
    BBa_K3739050 Composite J23100-B0030-LC1KR-2-GFP-B0010
    BBa_K3739051 Composite J23100-B0030-LamB-LC1KR-2-GFP-B0010
    BBa_K3739052 Composite J23100-B0030-OmpA-LC1KR-2-GFP-B0010
    BBa_K3739053 Composite J23100-B0034-his-rhlA-B0015-J23100-B0034-rhlB-B0015
    BBa_K3739054 Composite J23100-B0030-Aly01-PPO-his-B0010
    BBa_K3739055 Composite J23100-B0030-LMT-PPO-his-B0010
    BBa_K3739056 Composite J23100-B0030-LamB1-linker-LamB2-his-B0010
    BBa_K3739057 Composite J23100-B0030-LamB1-LC1KR2-LamB2-his-B0010
    BBa_K3739058 Composite J23100-B0030-pelB-Ag43-160N-his-GFP-B0010
    BBa_K3739059 Composite J23100-B0030-pelB-Ag43-160N-Tevp-B0010
    BBa_K3739060 Composite pDawn
    BBa_K3739061 Composite J23100-B0030-blrA-GFP-B0010
    BBa_K3739062 Composite pDawn-B0030-GFP-rrnB T1
    BBa_K3739063 Composite pDawn-B0030-blrA-rrnB T1
    BBa_K3739064 Composite K2332002-B0034-K2332004-K2332002-B0030-GFP
    BBa_K3739065 Composite J23106-B0034-K2332004-K2332002-B0030-GFP
    BBa_K3739066 Composite J23106-B0034-K2332004-K2332002-B0030-blrA-GFP
    BBa_K3739067 Composite J23100-B0034-phnC-B0034-phnD-B0034-phnE1-B0034-phnE2
    BBa_K3739068 Composite J23100-B0030-Aly01-his-3Spytag-elastin linker-hutH-B0010
    BBa_K3739069 Composite J23100-B0030-his-CBM-hutH
    BBa_K3739070 Composite J23100-B0030-pelB-Ag43-160N-his-GFP-B0010-J23100-B0030-pelB-Ag43-160N-Tevp-B0010
    BBa_K3739071 Composite K525998(T7-RBS)-his-CBM-hutH
    BBa_K3739084 Composite K525998(T7-RBS)-Aly01-his-hutH-FT
    BBa_K3739085 Composite K525998(T7-RBS)-LMT-his-hutH-FT
    BBa_K3739087 Composite K525998(T7-RBS)-his-hutH-FT
    BBa_K3739102 Composite J23100-B0034-INPNC-his-SpyCatcher-B0010
    BBa_K3739103 Composite J23100-B0034-his-SpyTag-GFP
    BBa_K3739104 Composite K525998(T7-RBS)-Aly01-his-hutH-B0010
    BBa_K3739105 Composite K525998(T7-RBS)-Aly01-his-GFP-B0010
    BBa_K3739106 Composite K525998(T7-RBS)-Aly01-his-CBM-hutH-B0010
    BBa_K3739107 Composite K525998(T7-RBS)-LMT-his-hutH-B0010
    BBa_K3739108 Composite K525998(T7-RBS)-LMT-his-GFP-B0010
  		• BBa_K3739109 Composite K525998(T7-RBS)-LMT-his-CBM-hutH-B0010
    BBa_K3739110 Composite K525998(T7-RBS)-LMT-PPO-his-B0010
    BBa_K3739111 Composite K525998(T7-RBS)-Aly01-PPO-his-B0010
    BBa_K3739112 Composite K525998(T7-RBS)-pelB-Ag43-160N-his-GFP-B0010
    BBa_K3739113 Composite K525998(T7-RBS)-pelB-Ag43-160N-Tevp-B0010
    BBa_K3739114 Composite K525998-Aly01-his-3SpyCatcher-B0010
    BBa_K3739115 Composite K525998-Aly01-his-3SpyTag-lecA-B0010
    BBa_K3739116 Composite K525998-Aly01-his-3SpyTag-hutH-B0010
    BBa_K3739117 Composite K2332002-B0034-K2332004-K2332002-B0030-blrA-GFP
    Part Collection

    This year, our team designed a Part Collection related to the secretion systems and surface display systems in Vibro natreigens. The secretion systems include 2 types of signal peptides: LMT and Aly01, and the surface display systems consist of 2 kinds of anchor proteins: OmpA and LamB. What's more, Ag43 autotransporter system was also utilized for surface display, and owing to a protease digestion site added between its translocation and cargo domains, we could release the cargo in certain conditions once the system was displayed, playing an effect both in secretion and display use.

    Users can also combine the signal peptides or anchor proteins with different proteins to verify their functions and treat it as a nice way to avoid the influence of components inside the cell. For our Part Collection, we've characterized two signal peptides, two anchor proteins and the Ag43 system combined with GFP and other target proteins separately to test its efficacy and uploaded complete and detailed quantitative data to the registry, which is believed to be very instructive for the design and characterization of many teams.


    SystemProteinNameTypeDescription
    Secretion systemLMTBBa_K3739009CodingLMT-his-hutH
    BBa_K3739010CodingLMT-his-GFP
    BBa_K3739011CodingLMT-his-CBM-hutH
    BBa_K3739017CodingLMT-PPO-his
    BBa_K3739083CodingLMT-his-hutH-FT
    BBa_K3739039CompositeJ23100-B0030-LMT-his-CBM-hutH-B0010
    BBa_K3739040CompositeJ23100-B0030-LMT-his-hutH-B0010
    BBa_K3739041CompositeJ23100-B0030-LMT-his-GFP-B0010
    BBa_K3739055CompositeJ23100-B0030-LMT-PPO-his-B0010
    BBa_K3739085CompositeK525998(T7-RBS)-LMT-his-hutH-FT
    BBa_K3739107CompositeK525998(T7-RBS)-LMT-his-hutH-B0010
    BBa_K3739108CompositeK525998(T7-RBS)-LMT-his-GFP-B0010
    BBa_K3739109CompositeK525998(T7-RBS)-LMT-his-CBM-hutH-B0010
    BBa_K3739110CompositeK525998(T7-RBS)-LMT-PPO-his-B0010
    Aly01BBa_K3739004CodingAly01-his-3Spycatcher
    BBa_K3739005CodingAly01-his-3Spytag-elastin linker-lecA
    BBa_K3739006CodingAly01-his-hutH
    BBa_K3739007CodingAly01-his-GFP
    BBa_K3739008CodingAly01-his-CBM-hutH
    BBa_K3739018CodingAly01-PPO-his
    BBa_K3739080CodingAly01-his-3*Spytag-elastin linker-hutH
    BBa_K3739082CodingAly01-his-hutH-FT
    BBa_K3739034CompositeJ23100-B0030-Aly01-his-3Spycatcher-B0010
    BBa_K3739035CompositeJ23100-B0030-Aly01-his-3Spytag-elastin linker-lecA-B0010
    BBa_K3739036CompositeJ23100-B0030-Aly01-his-CBM-hutH-B0010
    BBa_K3739037CompositeJ23100-B0030-Aly01-his-hutH-B0010
    BBa_K3739038CompositeJ23100-B0030-Aly01-his-GFP-B0010
    BBa_K3739054CompositeJ23100-B0030-Aly01-PPO-his-B0010
    BBa_K3739068CompositeJ23100-B0030-Aly01-his-3Spytag-elastin linker-hutH-B0010
    BBa_K3739084CompositeK525998(T7-RBS)-Aly01-his-hutH-FT
    BBa_K3739104CompositeK525998(T7-RBS)-Aly01-his-hutH-B0010
    BBa_K3739105CompositeK525998(T7-RBS)-Aly01-his-GFP-B0010
    BBa_K3739106CompositeK525998(T7-RBS)-Aly01-his-CBM-hutH-B0010
    BBa_K3739111CompositeK525998(T7-RBS)-Aly01-PPO-his-B0010
    BBa_K3739114CompositeK525998-Aly01-his-3SpyCatcher-B0010
    BBa_K3739115CompositeK525998-Aly01-his-3SpyTag-lecA-B0010
    BBa_K3739116CompositeK525998-Aly01-his-3SpyTag-hutH-B0010
    Surface display systemOmpABBa_K3739021CodingOmpA-GFP
    BBa_K3739025CodingOmpA-LC1KR2-GFP
    BBa_K3739049CompositeJ23100-B0030-OmpA-GFP-B0010
    BBa_K3739052CompositeJ23100-B0030-OmpA-LC1KR-2-GFP-B0010
    LamBBBa_K3739019CodingLamB-GFP
    BBa_K3739024CodingLamB-LC1KR2-GFP
    BBa_K3739026CodingLamB1-linker-LamB2-his
    BBa_K3739027CodingLamB1-LC1KR2-LamB2-his
    BBa_K3739048CompositeJ23100-B0030-LamB-GFP-B0010
    BBa_K3739051CompositeJ23100-B0030-LamB-LC1KR-2-GFP-B0010
    BBa_K3739056CompositeJ23100-B0030-LamB1-linker-LamB2-his-B0010
    BBa_K3739057CompositeJ23100-B0030-LamB1-LC1KR2-LamB2-his-B0010
    Autotransporter systemAg43BBa_K3739022CodingpelB-Ag43-160N-his-GFP
    BBa_K3739023CodingpelB-Ag43-160N-Tevp
    BBa_K3739058CompositeJ23100-B0030-pelB-Ag43-160N-his-GFP-B0010
    BBa_K3739059CompositeJ23100-B0030-pelB-Ag43-160N-Tevp-B0010
    BBa_K3739070CompositeJ23100-B0030-pelB-Ag43-160N-his-GFP-B0010-J23100-B0030-pelB-Ag43-160N-Tevp-B0010
    BBa_K3739112CompositeK525998(T7-RBS)-pelB-Ag43-160N-his-GFP-B0010
    BBa_K3739113CompositeK525998(T7-RBS)-pelB-Ag43-160N-Tevp-B0010

    Xiamen University, Fujian, China

    No.422, Siming South Road, Fujian, P.R.China 361005