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The COVID-19 pandemic is an unprecedented challenge to XMU-China this year. Our school shut down the laboratory for more than two weeks at the beginning of October. This announcement dramatically influenced the process of our experiments. We’ve tried our best to present the best as we can and hope it can inspire the iGEMers in the future.
The iGEMers of XMU-China are responsible for all the iGEM work this year, which include idea, lab work, wiki, paperwork and so on. Also, we communicate and collaborate with many iGEM teams this year. All the work done by our team members and help received from others are shown below.
Brainstorms
Start from Mar. 2021: All team members.
Guided by PI: Baishan Fang.
instructors: Aihui Zhang, Xiaoyan Zhuang, Jiacheng Huang and Junhong Chen
student leaders: Yixian Zheng and Shichen Geng.
advisors: Ruijie Mo, Ruomeng Bi, Zhichun Yang, Yangqi Deng, Shi Zhang, Meihao Ou, Xiaoyu Wang, Shuling Xiao, Yang Shi and Yang Liang, Limin Qiu.
Project
One of the best news under the pandemic was that some of our team members were approved to get back to school in June. After receiving the related trainings, this group of members started the lab work in July, while the rest started in August.
1. Molecular cloning and circuit construction
All student members.
Gene synthesis:
Genes from BBa_K3739031-BBa_K3739058, BBa_K3739066, BBa_K3739069, BBa_K3739102 and BBa_K3739103 were synthesized by Genscript BioTech.
BBa_K525998, BBa_K081005 and BBa_J23106 were acquired from Spring 2019 DNA Distribution Kit.
BBa_K3739064, BBa_K3739065 and BBa_K3739084-BBa_K3739117 were acquired by Standard BioBrick Assembly.
Guided by instructors: Aihui Zhang, Xiaoyan Zhuang and Junhong Chen.
student leaders: Yixian Zheng and Shichen Geng.
advisors: Zhichun Yang, Shi Zhang, Meihao Ou, Xiaoyu Wang, Shuling Xiao.
2. Characterization
All student members.
Guided by instructors: Aihui Zhang, Xiaoyan Zhuang and Junhong Chen.
student leaders: Yixian Zheng and Shichen Geng.
advisors: Zhichun Yang, Shi Zhang, Meihao Ou, Xiaoyu Wang, Shuling Xiao.
3. Proof of Concept
All student members.
Guided by student leaders: Yixian Zheng and Shichen Geng.
advisors: Zhichun Yang, Shi Zhang, Meihao Ou, Xiaoyu Wang, Shuling Xiao.
4. Hardware & Software
Function Design: All student members.
Construction: Hang Jin and Jielin Liu.
Art Design: Peiqi An.
Guided by instructors: Aihui Zhang, Xiaoyan Zhuang, Jiacheng Huang and Junhong Chen.
student leaders: Yixian Zheng and Shichen Geng.
advisors: Yansong Chen, Yang Liang Xiaoyu Wang and Zhichun Yang.
Model
Ruiyao Tang, Zhiyun Shang.
Guided by student leader: Yixian Zheng and Shichen Geng.
instructors: Jiacheng Huang and Junhong Chen.
advisors: Zhichun Yang.
Human practices
1. Human practices
Planners: Ruomeng Bi, Zichun Yang, Ziyan Han, Chong Li, Ruiyao Tang, Zhiyun Shang, Yuhao Zhang and Wenkai Yang.
Art Design: Yangqi Deng an Peiqi An.
Participants: All student members.
Guided by student leader: Yixian Zheng and Shichen Geng.
2. Science Communication
Planners: Yuhao Zhang, Ruiyao Tang, Zhiyun Shang, Zhiyan Han, Chong Li, Wenkai Yang and Ziyu Zhang.
Art Design: Peiqi An.
Participants: All student members.
Guided by advisors: Ruomeng Bi and Zhichun Yang.
student leader: Yixian Zheng and Shichen Geng.
Team
1. Collaboration
Yuhao Zhang, Wenkai Yang, Boyu Liu, Zhiyun Shang, Ziyu Zhang and Ziyan Han.
Guided by student leader: Yixian Zheng.
advisors: Ruomeng Bi.
2. Partnership
Ziyu Zhang, Yuhao Zhang, Chong Li and Wenkai Yang.
Guided by student leader: Yixian Zheng.
advisors: Shichen Geng, Xiaoyu Wang and Ruomeng Bi.
3. Safety
Shuling Xiao, Xiaoyu Wang and Shi Zhang, Zhichun Yang, Meihao Ou
Guided by instructors: Xiaoyan Zhuang.
advisor: Yang Shi.
Lab officer: Lila Wu.
student leader: Yixian Zheng and Shichen Geng.
Parts
1. Contribution
Zilan Chen, Ziyan Han and Boyu Liu.
Guided by instructor: Junhong Chen.
student leader: Yixian Zheng and Shichen Geng.
2. Engineering Success
Wenkai Yang, Chong Li, Zilan Chen and Boyu Liu.
Guided by instructor: Junhong Chen.
student leader: Yixian Zheng and Shichen Geng.
3. Improve a previous project
Xiaoyu Wang and Ziyan Han.
Guided by instructor: Junhong Chen.
student leader: Yixian Zheng and Shichen Geng.
Other Work
1. Wiki
Webpage: Yan Huang.
Art Design: Peiqi An and Yangqi Deng.
Paperwork: All student members.
Guided by instructor: Junhong Chen, Aihui Zhang, Xiaoyan Zhuang.
Advisors: Limin Qiu.
student leader: Yixian Zheng and Shichen Geng.
2.Art design
PowerPoint: Peiqi An, Yangqi Deng and Ruiyao Tang.
Logo, banner, animation and souvenirs: Peiqi An.
3. Presentation coaching
Instructors: Junhong Chen, Aihui Zhang and Xiaoyan Zhuang.
Advisors: Limin Qiu.
Team training
Our university don't have an iGEM or synthetic biology course. We learnt the knowledge from the textbooks and iGEM official website before we started to work. (Feb. 2021)
Guided by student leaders: Yixian Zheng and Shichen Geng.
advisors: Xiaoyu Wang, Shuling Xiao, Zhichun Yang, Shi Zhang, Meihao Ou, Ruijie Mo and Ruomeng Bi.
Supported by PI: Baishan Fang.
Acknowledgement
We would like to thank our PI Prof. Baishan Fang for providing us laboratory, materials and instruments. We also want to thank Professor Wenjing Hong for providing the laboratory for us, and we thank prof. Sheng Yang for providing us Vibrio natriegens VnDX as a gift. In the meantime, we thank Tingting Chen for her kindly help in the teaching of using equipments and the lending of reagents. Besides, we thank Ruxue Hou for providing the apparatus for us.
Last but not least, thanks to all members in iGEM 2021 XMU-China
who striked for the wet and dry lab work, love you guys more than three thousand times!
Wish you all the best and find your GEM in the future life after iGEM 2021!