Team:UESTC-China/Contribution
Part Contribution
This year we chose Pichia pastoris as a chassis organism to express our deinking-enzymes and cellulosome components, so in order to have better expression in yeast, we have done codon optimization on the sequences we used this year (BBa_K2155004,
BBa_K2155007, BBa_K2155005 and BBa_K1830009) in the previous iGEM part library. In addition, we found that the sequence of one part (BBa_K3819012) of the iGEM16_NWPU team had a lot of terminators after importing into Snapgene, so we
corrected that as well. And you can see more details in the Parts page.
Yeast Handbook
Why we make yeast handbook:
This year is our school's first use of Pichia pastoris as a chassis organism, and also the first time to use eukaryotes as a chassis organism. Pichia pastoris is a species of methylotrophic yeast. It was found in the 1960s, with its feature
of using methanol, as a source of carbon and energy. Since only a few members in the school’s existing laboratories have used yeast as a chassis organism, there is very little guidance available. We tried to form a complete protocol
by researching public information on the internet and combining comments from some forums. However, there are still many problems, such as low colony PCR efficiency, serious contamination of bacteria, low screening efficiency, and
many false positives.
The good news is that we have produced a product of intellectual contributions! Yeast handbook was edited by UESTC-China and UM_Mucau, and seven teams participated. Without exception, when every team first started using yeast, they were once or more misled by protocols that were lack of implementation, unsystematic, incomplete, and even incorrect. Suffering from groping experience all the time, We found it difficult to obtain substantive project results. So we have summarized several teams’ experience from selection of strains, selection of plasmids, to design of plasmids, experimental protocols that have been revised many times, and some experimental problems we encountered and solutions. We have completed this handbook at the end of the competition, hoping to record iGEMer's personal experience with yeast so that the teams using yeast will avoid some detours in the future.
The good news is that we have produced a product of intellectual contributions! Yeast handbook was edited by UESTC-China and UM_Mucau, and seven teams participated. Without exception, when every team first started using yeast, they were once or more misled by protocols that were lack of implementation, unsystematic, incomplete, and even incorrect. Suffering from groping experience all the time, We found it difficult to obtain substantive project results. So we have summarized several teams’ experience from selection of strains, selection of plasmids, to design of plasmids, experimental protocols that have been revised many times, and some experimental problems we encountered and solutions. We have completed this handbook at the end of the competition, hoping to record iGEMer's personal experience with yeast so that the teams using yeast will avoid some detours in the future.
Brief Introduction of this handbook：
All practical experience from UESTC-China, UM_Macau, HUST-China, CPU_CHINA, HiZJU, NWU-CHINA-B, and Jiangnan-China in using Yeast including Pichia pastoris and Saccharomyces cerevisiae!
Figure1. Several pages from YeastHandbook
How to use this handbook：
1. If you are considering whether using yeast ,please see part 1 in Preface and The problem we met section in Chapter 1 (Pichia pastoris) & Chapter 2 (Saccharomyces cerevisiae).
2. If you are considering which strain and vector to use , please see part 2&3 in Preface.
3. If you are carrying out experiments , you can find protocols of some specific vectors and some basic molecular experiments.
4. If you are willing to join or inherit Yeast Alliance, Welcome!! Please contact 249468856@qq.com.
2. If you are considering which strain and vector to use , please see part 2&3 in Preface.
3. If you are carrying out experiments , you can find protocols of some specific vectors and some basic molecular experiments.
4. If you are willing to join or inherit Yeast Alliance, Welcome!! Please contact 249468856@qq.com.
iGEM Team Handbook
Why we make team handbook:
During our whole journey of iGEM, one obvious problem was always with us. That is, we found that a lot of experience of elder iGEMer in UESTC-China is not left. Lots of problems that the new iGEMers encountered tripped every UESTC-China
team in previous years. So that is why this year a lot of thought was put into producing a work guide/ team manual that can be passed down.
Brief Introduction of this handbook：
Our Team Handbook is mainly about three parts:
1. Starting:
We briefly introduce our laboratory address, software that we use to do team work such as FeiShu and Evernote, and give an detial introduction of our iGEMers' life in UESTC-China in this session. The introduction includes every aspect
of our life as iGEMers, including:
1. The philosophy and criteria of an iGEMer;
2. How to communicate with mentors and teammates: contact information and team work advice;
3. How to buy experimental supplies: Detailed instructions of our school's reimbursement process and our lab's receiving and sorting process;
4. Network issues in our office area;
5. Life and finance;
6. Dormitory life;
2. Learning:
This section, we list the first skills to learn as a member of the wet lab, hardware, digital, wiki design, art, and human practice groups, along with recommended learning materials and guide handbooks. For example, in the wet lab group,
we listed the learning guidance including the following contents:
1. Tips for searching literature
2. Tips for reading literature
3. Manage your reading note
4. How to design an experiment
5. How to document your experiment
6. Training for your experimental operation
7. Scientific research drawing
3. Working:
This part explains what resources you need to use once you become an iGEMer and start working, such as a record of the location of laboratory supplies in the laboratory.
How to use this handbook:
The team manual that we wrote can not only be passed down to the next year UESTC-China Team, but the other iGEM Team has the same situation. Please click hereto
download the pdf of our handbook or contact us( 2139055344@qq.com/13712485116@163.com) to get the online documents.
Figure2. Partion of our Team Handbook
3D Printer
In the process of making hardware, in order to make our hardware more stable, we used 3D printing technology to make the bottom tube. They are placed under the guide rail to enhance the stability of the entire track system. If you want
to use this module in your project,click here
to download our 3D model and CAD drawing.
Figure3. 3D Printing pieces