Team:UESTC-China/Attributions
Abstract
The iGEMers of UESTC-China are responsible for all the iGEM work this year, which includes research, brainstorm, lab work, wiki, re-situ activities, interviews, surveys and so on. In addition, we have communicated and collaborated with many iGEM teams
this year, and we also have lauched a series of tailored connection with public, which we are proud of. All the detailed work done by our team members and the help received from others are shown below.
This summer, a local epidemic broke out in Chengdu. In addition, our laboratory has encountered serious contamination by bacteria. Progress has been hampered by the emergence of multidrug-resistant bacteria in the past month. Considering the progress
of the experiment and the serious biological safety problems that may be contributed to other laboratories, we have adopted the method of formaldehyde fumigation in the laboratory for thorough sterilization. After the laboratory returned
to normal, the team members had been promoting all aspects of work day and night and striving to do their best.
In addition, our team did not have seniors to guide the competition rules and team management methods at the beginning of the team's building. We worked hard in the mud and crossed the river by feeling the stones, wrote a series of easy-to-use iGEM introductory
guides about competition training and life tips for the future iGEMer.
PI
Yong Zhang
He is our primary PI. He gave us many insights and practical advices, especially on the design of the whole project and molecular cloning. In addition, he also helped us to comb the pathway and analyze the results. What’s more, he gave useful suggestions
on wiki and presentation.
Lixia Tang
She is one of our secondary PIs. At first, she gave us some guidance on background research, experiments of protein expression and detection of enzyme activity. Moreover, she came up with a series of valuable advices about the design of wiki and presentation.
Xuelian Zheng
She is one of our secondary PIs. She gave us a lot of detailed suggestions during the process of design and experiments. Also, she helped our team leader coordinate the team's work and supported our experiments by introducing teachers at Chengdu Institute
of Biology, Chinese Academy of Sciences to us. For wiki, she also raised some ideas about the style. She also helped us a lot with our patent application work.
Juan Feng
She is one of our secondary PIs. Meanwhile, she gave us much theoretical guidance on modeling and taught us the way of using various software to find models. At the same time, she had communicated with each member friendly to appease our tension. Her
dedication, trust and great desire to help us at any time inspired us to strive as much as possible.
Instructor
Ling Quan
She is our instructor. As she used to be a judge in iGEM, she helped us better understand the basic principles of the competition. She gave a lot guidance on human practice promotion and patent application. Also she gave us huge help on building the bridge
between academia and the public.
Advisor
Qiugu Meng
As an iGEMer in 2019, he expressed his interest in our project at the first time he heard our idea. He supported us a lot when we first entered our lab, and taught us about biosafty hand by hand.
Xu Tang
He helped us to check whether the construction strategy was feasible and analyse why our PCR results were not well-done.
Xingyang Qiu
He knew how to use Pichia pastoris best among all our PIs and advisers, offered us a lot of regents and taught us methods and matters which need attention in lab.
Acknowledgment
UESTC-China iGEM 2021 team gratefully acknowledges the following institutes:
(1) Office of Educational Administration, University of Electronic Science and Technology of China.
(2) Office of Students' Affairs, University of Electronic Science and Technology of China.
(3) BGI Genomics Company.
(4) School of Life Sciences, University of Electronic Science and Technology of China.
(5) Plant Genome Engineering Lab, University of Electronic Science and Technology of China.
(6) Protein Engineering Lab, University of Electronic Science and Technology of China.
(7) School of Medicine, University of Electronic Science and Technology of China.
(8) College of Life Sciences, Sichuan University.
(9) State key laboratory of pulp and paper engineering, South China University of Technology.
(10) Chengdu Yilukang Company.
(11) Sichuan Science and Technology Museum.
(12) The Chengdu Institute of Yeast, Chengdu Institute of biology, Chinese Academy of sciences.
(13) Physical Electronics Technology Association, University of Electronic Science and Technology of China.
(2) Office of Students' Affairs, University of Electronic Science and Technology of China.
(3) BGI Genomics Company.
(4) School of Life Sciences, University of Electronic Science and Technology of China.
(5) Plant Genome Engineering Lab, University of Electronic Science and Technology of China.
(6) Protein Engineering Lab, University of Electronic Science and Technology of China.
(7) School of Medicine, University of Electronic Science and Technology of China.
(8) College of Life Sciences, Sichuan University.
(9) State key laboratory of pulp and paper engineering, South China University of Technology.
(10) Chengdu Yilukang Company.
(11) Sichuan Science and Technology Museum.
(12) The Chengdu Institute of Yeast, Chengdu Institute of biology, Chinese Academy of sciences.
(13) Physical Electronics Technology Association, University of Electronic Science and Technology of China.
UESTC-China iGEM 2021 team gratefully acknowledges the following professionals:
(1) Special thanks go to Zhang Chunhui, PhD, professor and doctoral supervisor of South China University of Technology. He expressed his recognition of our program and said he was happy that someone was paying attention to recycling. He also gave us four
suggestions and invited us to visit his lab.
(2) Special thanks go to Deputy Director Pei. Through communication, we could understand his views on our project and gained some knowledge related to environmental protection policies and laws.
(3) Special thanks go to the teachers at Chengdu Institute of biology, Chinese Academy of sciences. They solved several key problems in our experiments, including expression, contamination of miscellaneous bacteria and protein purification. And they offered us a new strain of GS115.
(4) Special thanks go to Professor Luo, dean of the School of Mechanical and Electrical Engineering, University of Electronic Science and Technology of China, he was one of the judges of Zhongguancun Talent Maker Competition.
(5) Special thanks go to Professor Kejun Deng, she was responsible for a lab training camp, which improved our basic techniques on experiment design and practice.
(6) Special thanks go to Granny Hua, she was a well-known paper artist, focusing on making rare alpine vegetation with a variety of paper products. She was interested in our project, and her idea of protecting the planet resembles ours a lot. So she agreed to exhibit her artwork in our educational activities.
(7) Special thanks go to CPU-China, we got close from May this year. We often communicate from work to life, then back to work. They sent us a plate of strains they thought were false negative, we validated it. They also sent us a package of protein they expressed which we used as a positive control.
(8) Special thanks go to Yingying Cao、Bojun Hou、Kexin Gao、Zhangfeng Hu，they participated in our Fantacy Alf Night, and offered some help.
(9) Special thanks go to Jiayong He, PhD in Phonetics, Associate professor of University of Electronic Science and Technology of China. He gladly helped us in tailoring our presentation.
(10) Special thanks go to Jincao Yu, MA, Lecturer of School of Foreign Languages in University of Electronic Science and Technology of China. She was our language and speaking advisor.
(2) Special thanks go to Deputy Director Pei. Through communication, we could understand his views on our project and gained some knowledge related to environmental protection policies and laws.
(3) Special thanks go to the teachers at Chengdu Institute of biology, Chinese Academy of sciences. They solved several key problems in our experiments, including expression, contamination of miscellaneous bacteria and protein purification. And they offered us a new strain of GS115.
(4) Special thanks go to Professor Luo, dean of the School of Mechanical and Electrical Engineering, University of Electronic Science and Technology of China, he was one of the judges of Zhongguancun Talent Maker Competition.
(5) Special thanks go to Professor Kejun Deng, she was responsible for a lab training camp, which improved our basic techniques on experiment design and practice.
(6) Special thanks go to Granny Hua, she was a well-known paper artist, focusing on making rare alpine vegetation with a variety of paper products. She was interested in our project, and her idea of protecting the planet resembles ours a lot. So she agreed to exhibit her artwork in our educational activities.
(7) Special thanks go to CPU-China, we got close from May this year. We often communicate from work to life, then back to work. They sent us a plate of strains they thought were false negative, we validated it. They also sent us a package of protein they expressed which we used as a positive control.
(8) Special thanks go to Yingying Cao、Bojun Hou、Kexin Gao、Zhangfeng Hu，they participated in our Fantacy Alf Night, and offered some help.
(9) Special thanks go to Jiayong He, PhD in Phonetics, Associate professor of University of Electronic Science and Technology of China. He gladly helped us in tailoring our presentation.
(10) Special thanks go to Jincao Yu, MA, Lecturer of School of Foreign Languages in University of Electronic Science and Technology of China. She was our language and speaking advisor.
Team members
Table1. Attributions on wet lab
| Ying Zhou | Xi Chen | Yuwei Hu | Songwen Luo | Yujiao Peng | Yuanyi Wang | Liyan Wu | Weihan Long | Yuxuan Jiang | Zirui Wang | Shuheng Wang | |||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Wet lab | Expression | Cellulase assay | ▇▇▇▇ | ▇▇▇▇▇ | ▇▇▇▇▇ | ▇▇▇▇▇ | ▇▇▇▇▇ | ▇▇▇▇▇ | |||||
| Xylanase assay | ▇▇▇▇▇ | ▇▇▇ | ▇▇▇ | ▇▇▇▇▇▇ | ▇▇▇ | ||||||||
| Laccase assay | ▇▇▇▇▇ | ▇▇▇▇▇ | ▇▇▇▇▇ | ||||||||||
| Lipase assay | ▇▇▇▇▇ | ▇▇▇▇▇ | ▇▇▇▇▇ | ||||||||||
| Scaffoldin & SdbA expression | ▇▇▇▇▇ | ▇▇▇▇▇ | ▇▇▇▇▇ | ▇▇▇▇▇ | ▇▇▇▇▇ | ▇▇▇ | |||||||
| Cellulosome assembly | ▇▇▇▇ | ▇▇▇▇▇▇ | ▇▇▇▇ | ▇▇▇▇ | ▇▇▇▇ | ||||||||
| Deinking efficiency validation | Monoenzyme pulping deinking test | ▇▇▇▇▇▇ | ▇▇▇▇▇ | ▇▇▇▇▇ | ▇▇ | ▇▇ | |||||||
| Multienzyme pulping deinking test | ▇▇▇▇▇▇ | ▇▇▇▇▇ | ▇▇▇▇ | ▇▇▇▇ | |||||||||
| Monoenzyme in-situ deinking test | ▇▇▇▇▇▇ | ▇▇▇▇▇ | ▇▇▇▇▇ | ▇▇ | |||||||||
| Multienzyme in-situ deinking test | ▇▇▇▇▇▇ | ▇▇▇▇▇ | ▇▇▇▇▇ | ||||||||||
| Labeled according to the sextile punctuation can be copied below | Provide suggestions | Offer help | Engaged | Mainly participate or design | Mainly participate and succeed (characterize, obtain results) | Design, organization and participation | |||||||
| ▇ | ▇▇ | ▇▇▇ | ▇▇▇▇ | ▇▇▇▇▇ | ▇▇▇▇▇▇ |
Table2. Attributions on dry lab
| Zirui Wang | Jianyu Li | Ying Zhou | Dubai Li | Shuheng Wang | Xi Chen | Weihan Long | Liyan wu | Other members | |||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Dry lab | Modeling | Protein Structure Prediction and Molecular Docking | ▇▇▇▇▇▇ | ▇▇ | Yuwei Hu proposed “in-situ regeneration”prototype and engaged in part of experiment | ||||||
| Computer Vision to Complete Ink Recognition | ▇▇▇▇▇▇ | ||||||||||
| Improving the drying efficiency | ▇▇▇▇▇▇ | ||||||||||
| Location Decision-making Model of Deinkers | ▇▇▇▇▇▇ | ||||||||||
| Residual ink counting statistic method | ▇▇▇▇▇ | ▇▇▇▇▇▇ | |||||||||
| Hardware | Deinker 1st iteration | ▇▇▇▇▇▇ | ▇▇▇▇▇▇ | ||||||||
| Deinker 2nd iteration | ▇▇▇▇▇▇ | ▇▇ | |||||||||
| Deinker 3rd iteration | ▇▇▇▇▇▇ | ▇▇▇▇ | ▇▇▇▇ | ▇▇▇▇ | ▇▇ | ▇▇▇ | ▇▇▇▇ | ||||
| Deinker 4th iteration | ▇▇▇ | ▇▇▇▇▇▇ | ▇▇▇ | ▇▇▇▇ | ▇▇▇ |
Table3. Attributions on Integrated Human Practice
| Dubai Li | Yuxuan Jiang | Yuwei Hu | Ying Zhou | Zhe Zhang | Jianyu LI | Xi Chen | Liyan Wu | Yuanyi Wang | Weihan Long | Zirui Wang | Sen Chang | Songwen Luo | Yujiao Peng | Shuheng Wang | Other members | |||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Integrated Human Practice | Pratical Problems | Social research | ▇▇▇ | ▇▇▇ | ▇▇▇▇▇▇ | ▇▇ | ▇▇ | ▇▇ | ▇▇ | ▇▇ | ▇▇ | ▇▇ | ▇▇ | All members engaged | ||||
| School survey | ▇▇▇▇ | ▇▇▇▇ | ▇▇▇▇▇▇ | ▇▇ | ▇▇▇ | ▇▇▇ | ▇▇ | ▇▇ | ▇▇ | ▇▇ | ▇▇ | All members engaged | ||||||
| Waste paper saving series activities | ▇▇▇▇ | ▇▇▇ | ▇▇▇▇▇▇ | ▇▇▇ | ▇▇▇ | ▇▇▇ | ▇▇ | ▇▇ | ▇▇ | ▇▇ | ||||||||
| Demand research | ▇▇▇▇▇▇ | ▇▇ | ▇▇ | ▇▇ | ▇▇▇▇ | ▇▇ | ▇▇ | ▇▇ | ||||||||||
| Waste recycling station investigation | ▇▇▇▇▇▇ | ▇▇▇▇▇ | ▇▇▇ | |||||||||||||||
| Policies & Low | Patent Consulting | ▇▇▇▇ | ▇▇ | ▇▇ | ▇▇▇▇ | ▇▇▇▇▇ | ||||||||||||
| Patent Application | ▇▇ | ▇▇▇▇▇▇ | ▇▇▇▇▇▇ | |||||||||||||||
| Interview with Deputy Director Pei | ▇▇▇▇▇▇ | ▇▇▇ | ▇▇▇ | ▇▇▇▇▇ | ▇▇▇ | ▇▇▇ | ▇▇▇ | ▇▇ | ▇▇ | ▇▇ | ▇▇ | ▇▇ | All members online | |||||
| Research about domestic Agreements & Appeal | ▇▇▇ | ▇▇▇▇▇▇ | ▇▇▇ | |||||||||||||||
| Market & industry/ entrepreneurship | Stakholder engagement | ▇▇▇▇▇▇ | ▇▇▇▇ | |||||||||||||||
| Product assessment(Interviews— Yilukang company, Dean Luo) | ▇▇▇▇▇▇ | ▇▇▇ | ▇▇▇ | ▇▇▇ | ▇▇▇ | ▇▇▇ | ||||||||||||
| Business plan writing | ▇▇▇▇▇▇ | ▇▇▇ | ▇▇▇ | ▇▇▇ | ▇▇▇ | ▇▇▇ | ▇▇▇▇▇▇ | All members engaged | ||||||||||
| Chengdu Environment Expo | ▇▇▇▇ | ▇▇▇▇▇▇ | ▇▇▇▇ | ▇▇▇ | ||||||||||||||
| China-U.S. Young Maker Competition | ▇▇▇▇▇▇ | ▇▇▇ | ▇▇▇▇▇▇ | ▇▇▇▇▇ | ▇▇▇▇▇ | |||||||||||||
| Zhongguancun Talent Maker Competition | ▇▇▇▇ | ▇▇▇ | ▇▇▇▇▇▇ | ▇▇▇ | ▇▇▇ | ▇▇▇ | All members engaged | |||||||||||
| Science & Technology | Communicate with SCUT (a pioneer institution of drenking in China) | ▇▇▇ | ▇▇▇▇▇▇ | |||||||||||||||
| Communicate with teachers in Chengdu Institute of Yeast | ▇▇▇ | ▇▇▇ | ▇▇▇▇▇▇ | ▇▇▇ | ||||||||||||||
| Ethics & philosophy | Conversation with Hewlett Packard | ▇▇▇▇▇▇ |
Table4. Attributions on Public engagement
| Yuxuan Jiang | Zhe Zhang | Yuwei Hu | Dubai Li | Ying Zhou | Sen Chang | Weihan Long | Xi Chen | Yuanyi Wang | Yujiao Peng | Liyan Wu | Songwen Luo | Zirui Wang | Jianyu Li | Other members | |||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Public engagement | Education | Make Popular Science Video with Beijing 101 Middle School | ▇▇▇▇▇ | ||||||||||||||
| SNS*—Paper Elf Fantasy Night | ▇▇▇▇▇▇ | ▇▇▇ | ▇▇▇ | ▇▇▇▇▇ | ▇▇▇ | ▇▇▇ | ▇▇▇ | ▇▇▇ | ▇▇▇ | ▇▇▇ | ▇▇▇ | ||||||
| SNS*—Memes | ▇▇▇▇▇▇ | ▇ | ▇ | ▇▇▇▇▇ | ▇ | ▇ | ▇ | ▇ | ▇ | ||||||||
| SNS*—Science Popularization Video | ▇▇▇▇▇▇ | ||||||||||||||||
| SNS*—ASMR in Laboratory | ▇▇▇▇▇▇ | ▇ | |||||||||||||||
| SNS*—Pen and Ink Grocery Store | ▇▇▇▇▇▇ | ▇▇▇▇▇ | ▇▇ | ▇▇ | |||||||||||||
| Collaboration | IGEM Southwest Region Online Concept Exchange Meeting | ▇▇▇▇▇ | ▇▇▇▇▇▇ | ▇▇▇ | ▇▇▇ | ▇▇▇ | ▇▇▇ | All members online | |||||||||
| Taking Part in the Online Meet-up by SZU-China | ▇▇▇▇▇ | ▇▇▇▇▇▇ | ▇▇▇ | ▇▇▇ | ▇▇▇ | ▇▇▇ | All members online | ||||||||||
| Yeast Alliance | ▇▇▇▇▇ | ▇▇▇▇▇▇ | ▇▇▇ | ▇▇▇▇ | ▇▇▇▇ | ▇▇▇▇ | ▇▇▇▇ | ▇▇▇▇ | |||||||||
| Participate in ICII | ▇▇▇▇▇ | ||||||||||||||||
| Pen and Ink Grocery Store | See above | ||||||||||||||||
| Communication | with SCUT | See above | |||||||||||||||
| With deputy director Pei | See above | ||||||||||||||||
| with teachers in Chengdu Institute of Yeast | See above | ||||||||||||||||
| with Dean Luo | See above | ||||||||||||||||
| With Dean Lou | ▇▇▇▇▇▇ | ||||||||||||||||
| with Granny Hua | ▇▇▇ | ▇▇▇▇▇▇ | ▇▇▇ | ||||||||||||||
| with The Chengdu Yilukang Company | See above | ||||||||||||||||
| with CPU-China | ▇▇▇▇▇ | ▇▇▇▇▇ | ▇▇▇▇▇ | ||||||||||||||
| Be Interviewed by Beijing 101 Middle School | ▇▇▇▇▇ | ▇▇▇▇ | ▇▇▇▇ | ||||||||||||||
Table5. Attributions on WIKI
| Shuheng Wang | Yujiao Peng | Yuanyi Wang | Songwen Luo | Xi Chen | Yuwei Hu | Ying Zhou | Dubai Li | Yuxuan Jiang | Zirui Wang | Jianyu Li | Liyan Wu | ||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Wiki | WIki-making | ▇▇▇▇▇▇ | ▇▇▇ | ▇▇▇ | ▇▇▇ | ▇▇▇ | ▇▇ | ▇▇▇ | ▇▇▇ | ||||
| Wiki content | ▇▇▇▇ | ▇▇▇ | ▇▇▇ | ▇▇▇ | ▇▇▇▇ | ▇▇▇ | ▇▇▇▇▇ | ▇▇▇▇ | ▇▇▇▇ | ▇▇▇▇ | ▇▇▇▇ | ▇▇▇▇ |
Table6. Attributions on Team and Competition
| Sen Chang | Yuwei Hu | Ying Zhou | Zhe Zhang | Shuheng Wang | Dubai Li | Yuxuan Jiang | Songwen Luo | Liyan Wu | Help from Duan | Other members | |||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Team and competition | Art design | Promotion video | ▇▇▇▇▇▇ | ▇▇▇ | ▇ | ▇▇▇ | ▇ | ▇▇ | |||||
| Presentation video | ▇▇ | ▇▇▇▇▇ | ▇▇▇▇▇▇ | ▇ | ▇▇▇ | ▇▇▇▇▇▇ | ▇▇▇▇ | ▇▇▇ | ▇▇▇▇ | ChenXi, LiJianyu, WangZirui, LongWeihan, WangYuanyi, PengYujiao, ChengTao, YangShifa. | |||
| Wiki | ▇▇▇▇▇▇ | ▇▇▇▇ | ▇▇▇▇▇ | ▇▇▇▇ | ▇▇▇▇ | ▇▇▇▇ | |||||||
| Team culture | ▇▇▇▇▇▇ | ▇▇ | ▇▇ | ▇▇▇▇ | ▇▇ | ||||||||
| Team management | Daily work | ▇▇▇▇▇▇ | ▇▇▇▇▇▇ | ▇▇▇▇ | ▇ | ||||||||
| Funds management | ▇▇▇▇ | ▇▇▇▇ | ▇▇▇ | ▇▇▇▇▇ | |||||||||
| Recruitment | ▇▇▇▇▇▇ | ▇▇▇▇▇▇ | ▇▇▇▇ | ||||||||||
| iGEM Team Guideline | ▇▇ | ▇▇▇▇▇▇ | |||||||||||
Last but not the least, thanks to all our teachers, team members and all the people who helped us during this tough time, love you all!