Modeling
Rin 14b cell screening platform
In order to analyze the relationship between the dose of the compound (C3, No. 21072900) and its stimulation n the EC cells, we changed the doses of the compound and observed the reaction of the EC cell through the Patch-clamp technique.
The Patch-clamp technique is an electrophysiological experimental technique used to study ionic currents in tissue slices, isolated cells, or small pieces of cell membrane. It can be set up as a voltage clamp and a current clamp. In the voltage clamp, the device maintains the cell's transmembrane potential at a certain value through a negative feedback circuit to observe changes in the transmembrane current. In the current clamp, the microelectrode injects constant or changing current into the cell to record changes in the corresponding membrane potential, such as the formation of an action potential. In this project, we used the latter.
Figure 1. Current clamp map of the compound (C3, No. 21072900)
Model Build
According to the raw data (please refer to the end of the page) and its current clamp map (Fig. 1) as given below, the density of AP peaks could be used to indicate the intensity of the stimulus on the EC cells. In order to quantify the intensity of the stimulus, we counted the numbers of AP peaks during the corresponding drug administration time to further determine the AP peak density and the results are shown in table 1.
After observing the scatter plots in table 1 and tested several models by MATLAB, we chose to use the double exponential equation to build the model and its fitting degree is 0.9748 (Fig. 2)
Double exponential equation：
Figure 2. The model results
Figure 3. The fitting curve of the model
Conclusion
According to the model results (Fig. 2) and the fitting curve (Fig. 3), it indicates that the intensity of EC cells stimulated by the compound (C3, No. 21072900) is dose-dependent, and the data shows a relationship in accordance with the double exponential model. In addition, we could predict that the most optimal concentration of the compound would be 4 uM when the compound performs the strongest stimulus on EC cells. This value could be also used for the development of the new drugs based on the compound (C3, No. 21072900) to target Rin 14b cell functions.