Team:Shanghai HS/Notebook

Lab Notes

4th July

Transient transfection of plasmids in the B138 cell line

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HEK293F-hACE2-EGFP cells and 293T cell recovery

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5th July

PCR-preparation of fragment of gene with purpose for linear amplification

PDF-7.5A

B.1.1.7 Pseudo Virus Packaging--The Transient Transection of Cell

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6th July

1% Agarose Gel (50ml) Electrophoresis Detection
Protein Expression

PDF-7.6

7th July

1% Agarose Gel (50ml) Electrophoresis

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1% Agarose Gel (50ml) Recovery after Electrophoresis
Preparation Before Neutralization Test

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8th July

Plasmid Maxi-Prep

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Pseudovirus Packaging and neutralization Test

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9th July

Purification, Concentration and Testing of Proteins

PDF-7.9

10th July

ELISA Detection

PDF-7.10

11th July

ELISA Detection

PDF-7.11A

Pseudovirus Packaging and neutralization Test

PDF-7.11B

 

 

 

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